##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1NF_14A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2719198 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.75511823706843 12.0 12.0 12.0 2.0 27.0 2 14.344748341238851 12.0 12.0 12.0 12.0 22.0 3 13.756140965093385 12.0 12.0 12.0 12.0 22.0 4 13.746728263259977 12.0 12.0 12.0 12.0 22.0 5 22.43613778768593 22.0 22.0 22.0 22.0 27.0 6 26.77238398969108 27.0 22.0 32.0 22.0 32.0 7 34.9020012518397 37.0 37.0 37.0 27.0 41.0 8 37.519329228691696 41.0 37.0 41.0 27.0 41.0 9 38.49343703547884 41.0 37.0 41.0 32.0 41.0 10-11 38.87423736704719 41.0 41.0 41.0 37.0 41.0 12-13 39.04574069266012 41.0 41.0 41.0 37.0 41.0 14-15 38.99801834952806 41.0 41.0 41.0 37.0 41.0 16-17 38.90074610234341 41.0 41.0 41.0 37.0 41.0 18-19 38.82159298440202 41.0 41.0 41.0 37.0 41.0 20-21 38.83405658580214 41.0 41.0 41.0 37.0 41.0 22-23 38.83228694637169 41.0 41.0 41.0 34.5 41.0 24-25 38.84066662302635 41.0 41.0 41.0 37.0 41.0 26-27 38.79949253419574 41.0 41.0 41.0 34.5 41.0 28-29 38.747866466509606 41.0 41.0 41.0 32.0 41.0 30-31 38.64214135932727 41.0 41.0 41.0 32.0 41.0 32-33 38.690139886834274 41.0 41.0 41.0 32.0 41.0 34-35 38.6636269223499 41.0 41.0 41.0 32.0 41.0 36-37 38.586248960171346 41.0 41.0 41.0 32.0 41.0 38-39 38.48394857601396 41.0 41.0 41.0 32.0 41.0 40-41 38.53301892690418 41.0 41.0 41.0 32.0 41.0 42-43 38.28038690084355 41.0 41.0 41.0 32.0 41.0 44-45 38.39320343718994 41.0 41.0 41.0 32.0 41.0 46-47 38.39809605626365 41.0 41.0 41.0 32.0 41.0 48-49 38.34383281394 41.0 41.0 41.0 32.0 41.0 50-51 38.23070883400179 41.0 41.0 41.0 32.0 41.0 52-53 38.208065760566164 41.0 41.0 41.0 32.0 41.0 54-55 38.08165863611256 41.0 37.0 41.0 32.0 41.0 56-57 37.854384638411766 41.0 37.0 41.0 27.0 41.0 58-59 37.8439368887444 41.0 37.0 41.0 27.0 41.0 60-61 37.86091395330535 41.0 37.0 41.0 29.5 41.0 62-63 37.85937783861271 41.0 37.0 41.0 29.5 41.0 64-65 37.83549763569994 41.0 37.0 41.0 29.5 41.0 66-67 37.706551343447586 41.0 37.0 41.0 27.0 41.0 68-69 37.72081179818461 41.0 37.0 41.0 27.0 41.0 70-71 37.69735138816665 41.0 37.0 41.0 27.0 41.0 72-73 37.55473176282124 41.0 37.0 41.0 27.0 41.0 74-75 37.449735730903015 41.0 37.0 41.0 27.0 41.0 76-77 36.376762192381726 39.0 34.5 41.0 24.5 41.0 78-79 36.90882366786089 41.0 37.0 41.0 27.0 41.0 80-81 37.595838736274445 41.0 37.0 41.0 27.0 41.0 82-83 37.782667168775504 41.0 37.0 41.0 27.0 41.0 84-85 37.70820348499815 41.0 37.0 41.0 27.0 41.0 86-87 37.57952510262217 41.0 37.0 41.0 27.0 41.0 88-89 37.481784151062186 41.0 37.0 41.0 27.0 41.0 90-91 37.31497798247865 41.0 37.0 41.0 27.0 41.0 92-93 37.20326085117745 41.0 37.0 41.0 27.0 41.0 94-95 37.034937875064635 41.0 37.0 41.0 27.0 41.0 96-97 36.961094410925575 41.0 37.0 41.0 27.0 41.0 98-99 36.849176301247645 41.0 37.0 41.0 27.0 41.0 100 36.703745001283465 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3556192974921775 1101 2 -0.6045277178282955 1101 3 -0.07431718221848449 1101 4 -0.07317750404151724 1101 5 -1.0200613756648345 1101 6 -0.8946591351370863 1101 7 -0.30124379647008226 1101 8 0.0523987118582383 1101 9 -0.014780244403837628 1101 10-11 -0.034724047762807686 1101 12-13 0.017777225487087378 1101 14-15 0.130036978646622 1101 16-17 0.07410125757682096 1101 18-19 0.015172650907189222 1101 20-21 0.2196907182310781 1101 22-23 0.15320598368496263 1101 24-25 0.17944712845663702 1101 26-27 0.08240918903030803 1101 28-29 0.0677416974033278 1101 30-31 -0.024525519995194145 1101 32-33 -0.06339070290944449 1101 34-35 0.07196537261457792 1101 36-37 0.19559076483181315 1101 38-39 0.08774660158602643 1101 40-41 -0.06734954304047847 1101 42-43 0.22236596660037122 1101 44-45 0.07841616074234992 1101 46-47 0.10646377325083023 1101 48-49 0.2031388005592305 1101 50-51 0.14079795294032493 1101 52-53 0.1176696883166315 1101 54-55 -0.020884686103471495 1101 56-57 0.15877192348449398 1101 58-59 0.14497619536929562 1101 60-61 0.05172629504513537 1101 62-63 -0.024535185914643876 1101 64-65 -0.0013022955906123457 1101 66-67 0.0011890788303929867 1101 68-69 0.12988525680101048 1101 70-71 0.13525507619861088 1101 72-73 0.04547818367113621 1101 74-75 0.05342733017283763 1101 76-77 -0.38278913602277953 1101 78-79 -0.15228070113472114 1101 80-81 0.10230868369344392 1101 82-83 0.01769800377788755 1101 84-85 0.023112190252760456 1101 86-87 -0.015383114278861854 1101 88-89 -0.158847832426801 1101 90-91 -0.12895549818882301 1101 92-93 0.0399499116513411 1101 94-95 0.10329479198631475 1101 96-97 -0.17011243291683797 1101 98-99 -0.38391287054955825 1101 100 -0.5997712877375321 1102 1 1.6891043952595162 1102 2 0.512237768180059 1102 3 -0.0902472740014737 1102 4 -0.0018258003132860523 1102 5 -0.76335183554556 1102 6 -0.1321790973339425 1102 7 0.007305714951918674 1102 8 -0.40300082526024283 1102 9 -0.27043398694927134 1102 10-11 -0.38391486739423186 1102 12-13 -0.24063104153553638 1102 14-15 -0.24961520486417754 1102 16-17 -0.31620065017109766 1102 18-19 -0.1444861205597121 1102 20-21 -0.08007697870915109 1102 22-23 -0.2609938210907856 1102 24-25 -0.2758342738467334 1102 26-27 -0.008191936096018537 1102 28-29 -0.34882528762372544 1102 30-31 -0.2115539723773736 1102 32-33 -0.2119725677494273 1102 34-35 -0.1724509034311339 1102 36-37 -0.23161438964024228 1102 38-39 -0.32008461566820046 1102 40-41 -0.28584531605606855 1102 42-43 -0.17581423986823808 1102 44-45 -0.01680256974815819 1102 46-47 -0.40682872165298534 1102 48-49 -0.2616772023104943 1102 50-51 -0.10421675786220419 1102 52-53 -0.12303991388809266 1102 54-55 -0.26725354956031566 1102 56-57 -0.1047152782897598 1102 58-59 -0.42900344750139396 1102 60-61 -0.3108492853675884 1102 62-63 -0.25275423028159594 1102 64-65 -0.23036121813211707 1102 66-67 -0.34966718300464805 1102 68-69 -0.45725270741707646 1102 70-71 -0.4568662801615204 1102 72-73 -0.3456554966508847 1102 74-75 -0.7331334118172776 1102 76-77 -0.3354282850382049 1102 78-79 -0.3544718001506624 1102 80-81 -0.3959213635949723 1102 82-83 -0.46870683474820396 1102 84-85 -0.28862713363668746 1102 86-87 -0.4065875030303516 1102 88-89 -0.4737735195920081 1102 90-91 -0.48130853675423424 1102 92-93 -0.3202041637410886 1102 94-95 -0.2418551271301439 1102 96-97 -0.31489070162956523 1102 98-99 -0.5207347163499918 1102 100 -0.6960444361252058 1103 1 1.5983819384846072 1103 2 0.5378035945615789 1103 3 0.36798081400093174 1103 4 1.180627576763559 1103 5 2.879583905108529 1103 6 0.026088465475076816 1103 7 -0.4987555952047771 1103 8 -0.2834744301416947 1103 9 -0.1962245627496415 1103 10-11 -0.2660359922334834 1103 12-13 -0.3456999671890557 1103 14-15 -0.4690286542091826 1103 16-17 -0.19204060517125043 1103 18-19 -0.21859284244828103 1103 20-21 -0.25530148721033186 1103 22-23 -0.1766827189263438 1103 24-25 -0.27380226713102473 1103 26-27 -0.12533993842453128 1103 28-29 -0.08034476073142827 1103 30-31 -0.22039574014915786 1103 32-33 -0.4910984434851571 1103 34-35 -0.3297424819843897 1103 36-37 -0.3831236763918753 1103 38-39 -0.22022834536642932 1103 40-41 -0.3857552128469379 1103 42-43 -0.22500927146771943 1103 44-45 -0.560248234961108 1103 46-47 -0.42364798735733444 1103 48-49 -0.3281655720545231 1103 50-51 -0.06346749676561814 1103 52-53 -0.022994924743656497 1103 54-55 -0.20732117425841778 1103 56-57 -0.1842902067193677 1103 58-59 -0.32857432851412227 1103 60-61 -0.12076238725578037 1103 62-63 -0.23086801980235094 1103 64-65 -0.30206197554158365 1103 66-67 -0.23324345057959306 1103 68-69 -0.2725373810914036 1103 70-71 -0.3639036459569951 1103 72-73 -0.265275189368424 1103 74-75 -0.29405836792565054 1103 76-77 -0.5182585644277538 1103 78-79 -0.5536025691919875 1103 80-81 -0.27280520594272417 1103 82-83 -0.37925276489921345 1103 84-85 -0.33333154906869567 1103 86-87 -0.21855598957126432 1103 88-89 -0.252408101735476 1103 90-91 -0.7657281767911073 1103 92-93 -0.3809911721753494 1103 94-95 -0.1410039560739591 1103 96-97 -0.07455185717066115 1103 98-99 -0.3729277096075023 1103 100 -0.195551536415131 1104 1 1.8426411981434292 1104 2 -0.546672744483006 1104 3 0.09389768186681202 1104 4 0.08875595300860262 1104 5 -1.0443338445097474 1104 6 -0.4187804331902818 1104 7 -0.15885029368645576 1104 8 -0.1504078520817984 1104 9 -0.061499664464022885 1104 10-11 -0.01801220361815581 1104 12-13 -0.08356975839497949 1104 14-15 -0.11574917897412718 1104 16-17 -0.0016379154668229035 1104 18-19 0.05683323599028256 1104 20-21 0.04671084653510604 1104 22-23 0.11222775227897586 1104 24-25 -0.043538129784778334 1104 26-27 -0.08349310946076116 1104 28-29 -0.08204092942374785 1104 30-31 -0.08302531495894527 1104 32-33 -0.05802379228440202 1104 34-35 -0.17462616043101065 1104 36-37 -0.2046210480361239 1104 38-39 -0.12229548297230508 1104 40-41 0.03918120892127064 1104 42-43 0.02445194976655074 1104 44-45 -0.045549275011794066 1104 46-47 -0.1403534734251437 1104 48-49 -0.28897182170840097 1104 50-51 -0.22489705043061292 1104 52-53 -0.12586471954563905 1104 54-55 -0.05760963171673694 1104 56-57 -0.07499111716828111 1104 58-59 -0.2216371271214399 1104 60-61 -0.1733418186908935 1104 62-63 -0.19634113822112909 1104 64-65 -0.20351616577139708 1104 66-67 -0.4970860103082515 1104 68-69 -0.40114350387651143 1104 70-71 -0.22573399780209513 1104 72-73 -0.16269685472972384 1104 74-75 -0.43853594401275586 1104 76-77 -0.3366307854872588 1104 78-79 -0.28663782562299644 1104 80-81 -0.22634634803504383 1104 82-83 -0.26552864120358777 1104 84-85 -0.2625856627744838 1104 86-87 -0.22604378276652426 1104 88-89 -0.44817430573385764 1104 90-91 -0.26984233208165875 1104 92-93 -0.2214273411409735 1104 94-95 -0.18433874221304336 1104 96-97 -0.17538916234544644 1104 98-99 -0.3887552011513904 1104 100 -0.5294122310432741 1105 1 1.5844993106045564 1105 2 0.19741498452934536 1105 3 -0.058375290757920695 1105 4 -0.19444974979509055 1105 5 -1.2582925848503983 1105 6 0.4302509329265085 1105 7 -0.060095083122199355 1105 8 -0.09317887977744732 1105 9 -0.010052024322916964 1105 10-11 -0.06271423073224724 1105 12-13 -0.05339236414723558 1105 14-15 -0.16364449408049353 1105 16-17 -0.14235736510586605 1105 18-19 -0.08848587515797846 1105 20-21 -0.2296549568407471 1105 22-23 -0.09027880336828531 1105 24-25 -0.09576443804123613 1105 26-27 -0.07917413064429724 1105 28-29 -0.1943997610036874 1105 30-31 -0.30097586117582154 1105 32-33 -0.15314536341099938 1105 34-35 -0.07391069815775353 1105 36-37 -0.16951089923251317 1105 38-39 -0.12209846531298041 1105 40-41 -0.17078032779905783 1105 42-43 -0.013196183484602386 1105 44-45 -0.0299498816485837 1105 46-47 -0.1739841179854409 1105 48-49 -0.12547206373627517 1105 50-51 -0.18358115006949305 1105 52-53 -0.36936113439882945 1105 54-55 -0.20766404445959807 1105 56-57 -0.062615918235295 1105 58-59 -0.2175991792392722 1105 60-61 -0.21041343804895263 1105 62-63 -0.30135172476438044 1105 64-65 -0.291019430535961 1105 66-67 -0.28652410514835225 1105 68-69 -0.20434236373366588 1105 70-71 -0.5052043746342818 1105 72-73 -0.28028462030744095 1105 74-75 -0.40539031287717364 1105 76-77 -0.5354137683154647 1105 78-79 -0.28189181560060206 1105 80-81 -0.43534282034809024 1105 82-83 -0.30100783899434447 1105 84-85 -0.2786782532389296 1105 86-87 -0.4243944237051096 1105 88-89 -0.3827102042144759 1105 90-91 -0.40591049340108043 1105 92-93 -0.22628226370946436 1105 94-95 0.07469255425544219 1105 96-97 -0.23499627622391728 1105 98-99 -0.4000216860359629 1105 100 -0.4187074597857361 1106 1 1.1571164955928754 1106 2 -0.6894830157707172 1106 3 -0.15061439743001692 1106 4 -0.14122729657263733 1106 5 -1.2370353244178816 1106 6 -0.8209379562005239 1106 7 -0.3527439889669779 1106 8 -0.05962507220301916 1106 9 -0.17400255693324596 1106 10-11 -0.043817173484676175 1106 12-13 -0.05150583050811974 1106 14-15 -0.1201024746621826 1106 16-17 -0.14352803617344279 1106 18-19 -0.05806600247006344 1106 20-21 -0.025451943359378504 1106 22-23 -0.15329185607205886 1106 24-25 -0.20502649946824647 1106 26-27 -0.08219961449452029 1106 28-29 -0.15726835521248717 1106 30-31 0.04582054665151247 1106 32-33 -0.07882168207700602 1106 34-35 -0.1406136580565871 1106 36-37 -0.3465912944572338 1106 38-39 -0.28440508597011416 1106 40-41 -0.3202348576659588 1106 42-43 -0.08687901778598928 1106 44-45 -0.15091951107181956 1106 46-47 -0.612533531792586 1106 48-49 -0.3078189784543284 1106 50-51 -0.3841937055274016 1106 52-53 -0.3976179876041428 1106 54-55 -0.3165081621696899 1106 56-57 -0.25120198249146597 1106 58-59 -0.26616552512520286 1106 60-61 -0.2312270351512069 1106 62-63 -0.5161923703266709 1106 64-65 -0.8036490466707349 1106 66-67 -0.475779736672024 1106 68-69 -0.41838184334014983 1106 70-71 -0.7214810319418703 1106 72-73 -0.4679739273781962 1106 74-75 -0.48064718751548696 1106 76-77 -0.3664234774695103 1106 78-79 -0.3303655708784774 1106 80-81 -0.5244312743850017 1106 82-83 -0.45943079803586784 1106 84-85 -0.6412515005544464 1106 86-87 -0.5444484348931411 1106 88-89 -0.6492944252316875 1106 90-91 -0.784530636144936 1106 92-93 -0.7526830181514583 1106 94-95 -0.598616488331956 1106 96-97 -0.5308807094279331 1106 98-99 -0.870417982411638 1106 100 -0.8259055222415341 1107 1 1.4968432785040804 1107 2 -0.5200375107009272 1107 3 -0.17652335335042757 1107 4 -0.29678285212819233 1107 5 -1.216857987860248 1107 6 -0.206854548623415 1107 7 -0.3090012542505889 1107 8 -0.33877148092560105 1107 9 -0.29316932966244735 1107 10-11 -0.175355504484628 1107 12-13 -0.3255760863309547 1107 14-15 -0.35547093590694345 1107 16-17 -0.3374905324612598 1107 18-19 -0.2785742541352434 1107 20-21 -0.3080025351883222 1107 22-23 -0.20736041489433887 1107 24-25 -0.1855589333912846 1107 26-27 -0.29683709275170855 1107 28-29 -0.1819287429771137 1107 30-31 -0.3089072968850388 1107 32-33 -0.015473075738711373 1107 34-35 -0.22954582045954197 1107 36-37 -0.1258997667324948 1107 38-39 -0.08294905949690445 1107 40-41 -0.3733149136669809 1107 42-43 -0.2843797602237359 1107 44-45 -0.17652979489515985 1107 46-47 -0.3760840811965167 1107 48-49 -0.3107556379087484 1107 50-51 -0.2705078203294917 1107 52-53 -0.2711218694929016 1107 54-55 -0.35964294643849115 1107 56-57 -0.340507485015749 1107 58-59 -0.4280793174971933 1107 60-61 -0.26358883566879143 1107 62-63 -0.4103479004272188 1107 64-65 -0.3743151193872123 1107 66-67 -0.29230852204388214 1107 68-69 -0.4053946981193306 1107 70-71 -0.39099187153288995 1107 72-73 -0.2761933495495015 1107 74-75 -0.25837278252630824 1107 76-77 -0.19441507731677632 1107 78-79 -0.5132441080640078 1107 80-81 -0.6912273373437259 1107 82-83 -0.6442566433542609 1107 84-85 -0.43482029271430633 1107 86-87 -0.2004906053568476 1107 88-89 -0.3734338338270007 1107 90-91 -0.21936411796581723 1107 92-93 -0.36451365749640985 1107 94-95 -0.3349136331285223 1107 96-97 -0.32744470756124144 1107 98-99 -0.3528565722041819 1107 100 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0.002601529625259502 2223 20-21 0.12675110643367304 2223 22-23 0.21701733798345657 2223 24-25 0.15212230649309078 2223 26-27 0.2947463776997594 2223 28-29 0.06379300311711944 2223 30-31 0.15839112263924449 2223 32-33 0.23378497217019856 2223 34-35 0.18279531598897591 2223 36-37 0.09469150683415961 2223 38-39 0.13340366553666172 2223 40-41 0.2997017524949612 2223 42-43 0.2876538614782831 2223 44-45 0.21266243631124127 2223 46-47 0.1765110686912692 2223 48-49 0.13839052833563414 2223 50-51 0.24772704031475 2223 52-53 0.2627333539507646 2223 54-55 0.4095614992172969 2223 56-57 0.36279966969051713 2223 58-59 0.45771339353855467 2223 60-61 0.3621153458453321 2223 62-63 0.3472371716986018 2223 64-65 0.3090173727306009 2223 66-67 0.4911488064575096 2223 68-69 0.4922668909704413 2223 70-71 0.48594839207039797 2223 72-73 0.47663464879498463 2223 74-75 0.28428845325294105 2223 76-77 0.5260204031350568 2223 78-79 0.502562331609063 2223 80-81 0.48218335416908786 2223 82-83 0.3149630787754276 2223 84-85 0.34686661359783955 2223 86-87 0.3902341522889685 2223 88-89 0.4929922452001563 2223 90-91 0.5864380819810222 2223 92-93 0.6073155488335615 2223 94-95 0.638608098833295 2223 96-97 0.5535296892031099 2223 98-99 0.5992188747336229 2223 100 0.8207923897713485 2224 1 1.504922188474243 2224 2 -0.7554978300855968 2224 3 -0.003414437924726954 2224 4 -0.25186559444497725 2224 5 -1.0159561403862938 2224 6 0.6847274225093827 2224 7 0.4036882583207202 2224 8 0.3876589138263 2224 9 0.3034990881643651 2224 10-11 0.38039520439846797 2224 12-13 0.326168170200539 2224 14-15 0.30854221799983605 2224 16-17 0.3961406664772724 2224 18-19 0.4322886637016694 2224 20-21 0.3268098549432139 2224 22-23 0.36108250065259284 2224 24-25 0.43812550755084345 2224 26-27 0.48569626348142236 2224 28-29 0.4132660062365474 2224 30-31 0.4010754945989419 2224 32-33 0.43544224219787964 2224 34-35 0.5128644157450211 2224 36-37 0.48457301283011844 2224 38-39 0.44384571126949623 2224 40-41 0.48347167379336753 2224 42-43 0.6479729234291867 2224 44-45 0.6679489992290186 2224 46-47 0.5245672156722705 2224 48-49 0.4382869489589041 2224 50-51 0.5470155677612709 2224 52-53 0.4920567892531267 2224 54-55 0.6834891381829493 2224 56-57 0.6628949854670978 2224 58-59 0.5958590416657614 2224 60-61 0.572811890389147 2224 62-63 0.736740459072486 2224 64-65 0.5890144085404287 2224 66-67 0.337875417123513 2224 68-69 0.5779452020645408 2224 70-71 0.8193687664282194 2224 72-73 0.6616094832887924 2224 74-75 0.36713589581924566 2224 76-77 0.7139470491304607 2224 78-79 0.6025311507372564 2224 80-81 0.6753662520631067 2224 82-83 0.5912312904788237 2224 84-85 0.4641436603276645 2224 86-87 0.6672154758835234 2224 88-89 0.6674800023587011 2224 90-91 0.6299323871226505 2224 92-93 0.5551958183005326 2224 94-95 0.5245963451166773 2224 96-97 0.5437215987705528 2224 98-99 0.5965059381512319 2224 100 0.8118007898714481 2225 1 1.5526212787265248 2225 2 1.3711061582205186 2225 3 0.9930957607131319 2225 4 1.0597555044101643 2225 5 3.285366458808646 2225 6 -0.04093913826356044 2225 7 0.24873122570411255 2225 8 0.3929107063121364 2225 9 0.22839061411972494 2225 10-11 0.07224765422963486 2225 12-13 0.25911314835827426 2225 14-15 0.04303666260066308 2225 16-17 0.09274626999776814 2225 18-19 0.0903618536897497 2225 20-21 0.006926029740242257 2225 22-23 0.2232378601483802 2225 24-25 0.12201732974052959 2225 26-27 0.1939531924830291 2225 28-29 0.09582577922185465 2225 30-31 0.18060292040296133 2225 32-33 0.22444866418302922 2225 34-35 0.19769202344496506 2225 36-37 0.2667802870586726 2225 38-39 0.2242884810739625 2225 40-41 0.14010608308735328 2225 42-43 0.2848854645970462 2225 44-45 0.16697886528016426 2225 46-47 0.21860707460574957 2225 48-49 0.2229088346446204 2225 50-51 0.2831170166286796 2225 52-53 0.2592251441874396 2225 54-55 0.34074835395280445 2225 56-57 0.3367414811221039 2225 58-59 0.3636331019930097 2225 60-61 0.1933539657184511 2225 62-63 0.15738953397687538 2225 64-65 0.32626778675125223 2225 66-67 0.42984646122221193 2225 68-69 0.3861225767312817 2225 70-71 0.3801985107686008 2225 72-73 0.5244057343829098 2225 74-75 0.2668676093807534 2225 76-77 0.6470627641610633 2225 78-79 0.4117136780048938 2225 80-81 0.41370587870060405 2225 82-83 0.21459412660762212 2225 84-85 0.34574057117989554 2225 86-87 0.34575614644546704 2225 88-89 0.5093297378254675 2225 90-91 0.377429139938549 2225 92-93 0.4655214280942275 2225 94-95 0.4710039005667852 2225 96-97 0.4333650921374499 2225 98-99 0.4760571900429156 2225 100 0.46618512510684695 2226 1 1.3182868619374126 2226 2 1.384261443204469 2226 3 0.7596923333228833 2226 4 0.8184926375295039 2226 5 3.078211198111376 2226 6 -0.3521844107938428 2226 7 0.13546725782729396 2226 8 0.1963899756068912 2226 9 0.415805863726078 2226 10-11 0.14482249430859895 2226 12-13 0.1914948148832778 2226 14-15 0.19531465198738118 2226 16-17 0.10964440221714966 2226 18-19 0.17924823520380784 2226 20-21 0.2237413173957279 2226 22-23 0.21016285613433183 2226 24-25 0.2888300538020232 2226 26-27 0.17659715515170404 2226 28-29 0.23101218623692432 2226 30-31 0.2854856224849982 2226 32-33 0.20975421085596224 2226 34-35 0.15425327242795817 2226 36-37 0.3100265998684151 2226 38-39 0.31070318692854215 2226 40-41 0.2526272833350731 2226 42-43 0.3791193007995801 2226 44-45 0.33971484073442326 2226 46-47 0.3335716160529998 2226 48-49 0.09350653658498942 2226 50-51 0.141322777429167 2226 52-53 0.34436361874107035 2226 54-55 0.3390558759423712 2226 56-57 0.42103548757426523 2226 58-59 0.27264406742901315 2226 60-61 0.3635491159383193 2226 62-63 0.460710939851829 2226 64-65 0.3871948025794225 2226 66-67 0.403874724171402 2226 68-69 0.5687002232710299 2226 70-71 0.5145375825047083 2226 72-73 0.19150551350905687 2226 74-75 0.3816164378857536 2226 76-77 0.5598484530999741 2226 78-79 0.47905085942773695 2226 80-81 0.2827397883028837 2226 82-83 0.3138492338940324 2226 84-85 0.3331211110727921 2226 86-87 0.4062890096966143 2226 88-89 0.24156420008463897 2226 90-91 0.31654546908782777 2226 92-93 0.3182193409239389 2226 94-95 0.41092995437257684 2226 96-97 0.5913226228297361 2226 98-99 0.37035818566186407 2226 100 0.6944418002333705 2227 1 1.3259796598631421 2227 2 1.8832086996983186 2227 3 0.7317745229748471 2227 4 -0.09455787166572804 2227 5 3.2638130477227563 2227 6 0.37130189127163504 2227 7 0.3619139536985898 2227 8 0.4983895066606152 2227 9 0.4157866083009836 2227 10-11 0.2801882178797612 2227 12-13 0.34629522643816557 2227 14-15 0.2672010327104175 2227 16-17 0.273363228024742 2227 18-19 0.1991272463815008 2227 20-21 0.14172087460956106 2227 22-23 0.34936373213689365 2227 24-25 0.304948719022768 2227 26-27 0.3448630729129789 2227 28-29 0.4907165796774038 2227 30-31 0.40911465325892493 2227 32-33 0.21240736987032705 2227 34-35 0.23161151671489222 2227 36-37 0.33625990132521366 2227 38-39 0.25967392481688023 2227 40-41 0.35170360229761144 2227 42-43 0.4004949526724033 2227 44-45 0.2661151531835202 2227 46-47 0.39751385591220156 2227 48-49 0.34845645884760046 2227 50-51 0.40223131654563105 2227 52-53 0.4036162253803468 2227 54-55 0.4921836215444628 2227 56-57 0.3724305346018042 2227 58-59 0.47368587112728733 2227 60-61 0.41876166175089224 2227 62-63 0.4506577335338662 2227 64-65 0.32578814436015335 2227 66-67 0.34956735373995684 2227 68-69 0.3512937790766628 2227 70-71 0.6106903144916842 2227 72-73 0.5031709271530715 2227 74-75 0.458449673635549 2227 76-77 0.7908091829385171 2227 78-79 0.705270239041802 2227 80-81 0.4557106754633651 2227 82-83 0.3898049845863696 2227 84-85 0.4891148477771736 2227 86-87 0.3524452178842239 2227 88-89 0.4759244496622159 2227 90-91 0.5343492055263894 2227 92-93 0.45856957836206647 2227 94-95 0.657909493864608 2227 96-97 0.5259835126416732 2227 98-99 0.5487881562368528 2227 100 0.7458464579332613 2228 1 1.1101102242203655 2228 2 1.585961048718005 2228 3 -0.13281985112659278 2228 4 -0.043960874704012554 2228 5 3.232960932106913 2228 6 0.46921216136142263 2228 7 0.6851724516190245 2228 8 0.7685699418244951 2228 9 0.464026272032001 2228 10-11 0.40823572308185163 2228 12-13 0.32427884816290486 2228 14-15 0.44145775533019815 2228 16-17 0.4052642514263951 2228 18-19 0.35648223578321137 2228 20-21 0.3530428431153183 2228 22-23 0.4500903106850416 2228 24-25 0.4083525967979469 2228 26-27 0.3351569031481816 2228 28-29 0.3577423915056599 2228 30-31 0.408268106452482 2228 32-33 0.30900899507139457 2228 34-35 0.48360225213365027 2228 36-37 0.3684875077989389 2228 38-39 0.370836999103517 2228 40-41 0.3780027646804811 2228 42-43 0.5100938988018129 2228 44-45 0.4492797764060441 2228 46-47 0.4399247536957702 2228 48-49 0.44668627292633545 2228 50-51 0.35037783480748885 2228 52-53 0.4306582051553889 2228 54-55 0.4038708121181074 2228 56-57 0.640203839664025 2228 58-59 0.5211283629154408 2228 60-61 0.41470372317592563 2228 62-63 0.4622452966049835 2228 64-65 0.45108922552618225 2228 66-67 0.6276238814598543 2228 68-69 0.5693805259011029 2228 70-71 0.6114284054011847 2228 72-73 0.6638250858585693 2228 74-75 0.6828205323716148 2228 76-77 0.6680106899437561 2228 78-79 0.7448881231744409 2228 80-81 0.6295323928747294 2228 82-83 0.7207163331582223 2228 84-85 0.5972490062868587 2228 86-87 0.5153439494482015 2228 88-89 0.6352320672161653 2228 90-91 0.70230907161163 2228 92-93 0.6864471496319453 2228 94-95 0.840612103406329 2228 96-97 0.7138427991757439 2228 98-99 0.8610818051776832 2228 100 0.8564670839322588 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 25.0 13 45.0 14 145.0 15 481.0 16 1720.0 17 4559.0 18 7362.0 19 9651.0 20 11324.0 21 12826.0 22 15017.0 23 16977.0 24 19236.0 25 21787.0 26 25179.0 27 28056.0 28 31642.0 29 35541.0 30 41270.0 31 46920.0 32 55528.0 33 66493.0 34 81523.0 35 105504.0 36 148029.0 37 243538.0 38 597385.0 39 1090375.0 40 1060.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.648476664753442 20.765914316332186 19.261531596391638 37.324077422522734 2 25.380641958054817 48.07649800939675 10.834913250154008 15.707946782394425 3 36.11204480144513 16.009426308786633 24.64763507475366 23.230893815014575 4 19.65965700180715 17.783442029598433 21.6831948243563 40.87370614423812 5 17.311722059224817 35.41632496052145 26.13027811876884 21.141674861484894 6 17.240267167010273 27.944452739373887 30.909702051855 23.90557804176084 7 20.378913194258015 26.65197606058845 36.42191557952014 16.547195165633397 8 20.610783032349982 27.757596173577646 34.416728756052336 17.214892038020032 9 20.686320010532516 28.181765358756515 27.67650608745667 23.455408543254297 10-11 19.35337183978511 30.336242524450224 28.788469982693428 21.521915653071236 12-13 24.25005461169065 26.49071527707802 27.351336680889 21.90789343034233 14-15 21.804204769200332 27.39107266186574 28.538010104449913 22.266712464484012 16-17 20.908021409253756 29.738290481237485 28.193459983421583 21.160228126087176 18-19 21.517061280568754 29.632873369280205 29.729556288287945 19.120509061863096 20-21 22.690716159691206 29.083005356726506 29.93807365259904 18.288204830983254 22-23 22.121320330479797 30.31125353872723 28.58890746462744 18.978518666165538 24-25 22.274085962110888 28.37092039638158 26.493712484342808 22.861281157164722 26-27 20.131266645532982 26.383863918699557 30.137654558439657 23.3472148773278 28-29 19.4395308007617 28.148515769057358 30.845094818817103 21.56685861136384 30-31 20.037941542587046 30.152198327313478 28.149159988002587 21.66070014209689 32-33 20.524967288148932 30.147731058937232 28.75539037613296 20.571911276780874 34-35 21.35322620861004 29.29965011742433 29.95088993151657 19.39623374244906 36-37 21.9910797227712 28.09988459832642 28.81180406869967 21.097231610202712 38-39 21.57807093934615 29.26047599854073 28.9448463647704 20.21660669734272 40-41 20.78521863513774 29.869019899023723 29.837134976013672 19.508626489824866 42-43 20.414317548818225 30.080286643710114 29.45138716630973 20.054008641161932 44-45 20.59892291771324 30.032384548679424 28.737719724712953 20.630972808894388 46-47 19.850261507266726 28.090052720703472 28.94852252025962 23.111163251770183 48-49 20.876817355705615 28.000038982082216 30.38451043285557 20.738633229356598 50-51 21.860489747344623 30.797573402157546 28.153356246952228 19.188580603545606 52-53 20.439609379941775 30.367145931617216 28.187137664370372 21.006107024070634 54-55 22.29235028655482 28.415310093843438 28.15893539394345 21.13340422565829 56-57 21.725733850381523 28.721313489245908 29.365931864611934 20.18702079576063 58-59 21.200621807128346 28.062237354439812 28.916330234534865 21.82081060389698 60-61 19.605441906682643 30.35169612760252 29.8025444304452 20.240317535269643 62-63 20.464315158037483 31.262554643675696 27.166653579095247 21.10647661919158 64-65 19.897238026012467 30.252962593913313 28.77092552892003 21.078873851154192 66-67 20.1277913561278 30.295053909277662 29.546303726319305 20.03085100827523 68-69 20.709580545440236 29.94870178633553 29.385741678244838 19.9559759899794 70-71 19.609292887093915 31.077159515415943 29.23374833314823 20.079799264341915 72-73 20.11120558341099 30.836206116656456 28.523925069082868 20.528663230849684 74-75 20.041460754237093 29.13910645712449 30.586996607087823 20.23243618155059 76-77 19.463532997597085 30.43325642340131 28.77861413549142 21.32459644351018 78-79 19.571928929044518 29.608950874485785 28.420824816729052 22.39829537974064 80-81 20.605697753507517 29.597459835991874 28.417165451018672 21.379676959481937 82-83 20.096201159312415 28.48665304990663 30.094719104677186 21.32242668610377 84-85 20.320973316396966 29.184358770490416 28.392029561657516 22.1026383514551 86-87 21.56349041151104 28.791044271141715 29.1361092498597 20.50935606748755 88-89 21.14643729511422 29.32679047277911 28.826643002826568 20.700129229280105 90-91 21.4690875765575 29.68207905419171 27.928878294261768 20.919955074989023 92-93 21.98381655179211 28.900727346813287 28.854279828096374 20.26117627329823 94-95 21.967616922342543 29.641920154398466 28.121710886812952 20.268752036446042 96-97 21.35898158206942 29.440463695545528 28.487131132047022 20.713423590338035 98-99 22.38816371591918 29.747962450693183 27.922295470944007 19.941578362443632 100 22.472765866994607 31.278560810944995 26.434375135609837 19.814298186450564 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3.0 1 9.5 2 18.0 3 22.5 4 28.5 5 30.5 6 33.0 7 41.0 8 45.0 9 51.5 10 69.0 11 83.0 12 102.5 13 121.5 14 126.0 15 166.5 16 233.5 17 318.0 18 464.0 19 732.5 20 1199.0 21 1894.0 22 2975.5 23 4693.0 24 7133.5 25 10461.0 26 15187.0 27 20643.0 28 26233.0 29 32565.5 30 39989.0 31 48600.0 32 57995.0 33 68136.0 34 80515.5 35 94348.0 36 107144.5 37 116723.0 38 123224.5 39 128104.5 40 130984.0 41 131114.5 42 129320.5 43 127313.0 44 127102.0 45 124818.0 46 115527.0 47 104541.0 48 97429.5 49 93178.5 50 89144.0 51 83173.5 52 73849.5 53 62844.5 54 52401.5 55 42690.0 56 32818.0 57 24032.5 58 17310.0 59 12461.0 60 9606.5 61 7960.0 62 6639.5 63 5171.0 64 3748.5 65 3074.0 66 3329.0 67 3262.0 68 2416.0 69 2148.5 70 2593.5 71 2581.0 72 1733.5 73 787.0 74 353.0 75 208.5 76 150.0 77 121.0 78 87.5 79 67.5 80 52.0 81 39.5 82 34.0 83 31.0 84 22.0 85 19.0 86 21.0 87 12.0 88 12.0 89 17.5 90 14.0 91 13.0 92 12.5 93 12.5 94 15.5 95 14.0 96 13.0 97 17.5 98 46.0 99 94.5 100 96.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.323559373021014 2 0.006362170022190366 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 4.413065911345919E-4 30-31 0.010241990469248653 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0011032664778364797 40-41 0.0016365119421241116 42-43 1.2871442241425596E-4 44-45 0.0 46-47 0.0021145940825199197 48-49 0.0 50-51 0.0 52-53 0.0012503686748813437 54-55 0.019160061165093528 56-57 0.011124603651517836 58-59 0.012816278917533773 60-61 1.4710219704486396E-4 62-63 0.012264645678615533 64-65 5.5163323891823986E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.2871442241425596E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2719198.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.21245260066237 #Duplication Level Percentage of deduplicated Percentage of total 1 86.86877289834909 65.33613464094785 2 8.266376008733332 12.434688274722165 3 2.2014325099076895 4.967254149149679 4 0.9131987792496463 2.7473567959718705 5 0.48885168585615596 1.8383867125605007 6 0.3122390224487334 1.409055760560151 7 0.19634277342178322 1.0337195077627923 8 0.13928423313802643 0.8380727026330735 9 0.10404908743275802 0.7043208351010638 >10 0.47749114588228214 6.108550426809138 >50 0.022994184018737783 1.1718816554655909 >100 0.008521289645283333 1.172430662465802 >500 4.463819165400174E-4 0.23814787585033037 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 1.8387774630607995E-5 0.0 0.0 0.0 0.0 12-13 3.677554926121599E-5 0.0 0.0 0.0 0.0 14-15 3.677554926121599E-5 0.0 0.0 0.0 0.0 16-17 3.677554926121599E-5 0.0 0.0 0.0 0.0 18-19 3.677554926121599E-5 0.0 0.0 0.0 0.0 20-21 3.677554926121599E-5 0.0 0.0 0.0 0.0 22-23 3.677554926121599E-5 1.8387774630607995E-5 0.0 0.0 1.8387774630607995E-5 24-25 3.677554926121599E-5 3.677554926121599E-5 0.0 0.0 3.677554926121599E-5 26-27 3.677554926121599E-5 3.677554926121599E-5 0.0 0.0 3.677554926121599E-5 28-29 3.677554926121599E-5 3.677554926121599E-5 0.0 0.0 3.677554926121599E-5 30-31 3.677554926121599E-5 3.677554926121599E-5 0.0 0.0 3.677554926121599E-5 32-33 3.677554926121599E-5 3.677554926121599E-5 0.0 0.0 3.677554926121599E-5 34-35 3.677554926121599E-5 5.5163323891823986E-5 0.0 0.0 3.677554926121599E-5 36-37 3.677554926121599E-5 7.355109852243198E-5 0.0 0.0 3.677554926121599E-5 38-39 3.677554926121599E-5 7.355109852243198E-5 0.0 0.0 3.677554926121599E-5 40-41 3.677554926121599E-5 7.355109852243198E-5 0.0 0.0 3.677554926121599E-5 42-43 3.677554926121599E-5 7.355109852243198E-5 0.0 0.0 7.355109852243198E-5 44-45 3.677554926121599E-5 7.355109852243198E-5 0.0 0.0 7.355109852243198E-5 46-47 3.677554926121599E-5 7.355109852243198E-5 0.0 0.0 7.355109852243198E-5 48-49 3.677554926121599E-5 7.355109852243198E-5 0.0 0.0 7.355109852243198E-5 50-51 3.677554926121599E-5 7.355109852243198E-5 0.0 0.0 7.355109852243198E-5 52-53 5.5163323891823986E-5 7.355109852243198E-5 0.0 0.0 7.355109852243198E-5 54-55 7.355109852243198E-5 7.355109852243198E-5 0.0 0.0 7.355109852243198E-5 56-57 9.193887315303998E-5 7.355109852243198E-5 1.2871442241425596E-4 0.0 7.355109852243198E-5 58-59 1.2871442241425596E-4 7.355109852243198E-5 2.574288448285119E-4 0.0 7.355109852243198E-5 60-61 1.6548997167547196E-4 7.355109852243198E-5 2.574288448285119E-4 0.0 7.355109852243198E-5 62-63 2.942043940897279E-4 7.355109852243198E-5 2.574288448285119E-4 0.0 7.355109852243198E-5 64-65 4.229188165039839E-4 7.355109852243198E-5 2.574288448285119E-4 0.0 7.355109852243198E-5 66-67 4.964699150264159E-4 7.355109852243198E-5 2.574288448285119E-4 0.0 7.355109852243198E-5 68-69 5.884087881794558E-4 7.355109852243198E-5 2.574288448285119E-4 0.0 7.355109852243198E-5 70-71 6.987354359631039E-4 7.355109852243198E-5 2.574288448285119E-4 0.0 7.355109852243198E-5 72-73 7.538987598549278E-4 7.355109852243198E-5 2.574288448285119E-4 0.0 7.355109852243198E-5 74-75 8.458376330079678E-4 7.355109852243198E-5 2.574288448285119E-4 0.0 7.355109852243198E-5 76-77 9.745520554222237E-4 7.355109852243198E-5 2.574288448285119E-4 0.0 7.355109852243198E-5 78-79 0.0013239197734037757 7.355109852243198E-5 2.574288448285119E-4 0.0 7.355109852243198E-5 80-81 0.0016181241674935035 7.355109852243198E-5 2.574288448285119E-4 0.0 7.355109852243198E-5 82-83 0.0018020019137995834 7.355109852243198E-5 2.574288448285119E-4 0.0 7.355109852243198E-5 84-85 0.0018755530123220154 7.355109852243198E-5 2.574288448285119E-4 0.0 7.355109852243198E-5 86-87 0.0018755530123220154 7.355109852243198E-5 2.574288448285119E-4 0.0 7.355109852243198E-5 88 0.0018755530123220154 7.355109852243198E-5 2.574288448285119E-4 0.0 7.355109852243198E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 11515 0.0 80.58697 8 CACCGGC 11510 0.0 78.33829 9 CGCTGCA 85 0.0 74.77158 1 CTTCACC 13105 0.0 73.88976 6 CCTTCAC 14360 0.0 71.51794 5 GCTGCCG 30 7.5927645E-5 70.6176 1 GTGTGGC 4860 0.0 70.02027 7 CGGGGCA 1220 0.0 69.637115 94 CGTAGTG 2180 0.0 69.54535 4 ACGCAGC 35 3.6670572E-6 67.05883 2 TTCACCG 14295 0.0 66.983536 7 ATGCAGC 85 0.0 66.269905 2 ACCTTCA 5615 0.0 64.11619 4 CAGCTGC 75 1.2732926E-11 63.555843 1 TGTGGCA 5505 0.0 61.731003 8 GCCTTCA 4375 0.0 61.36773 4 GCGTAGT 1475 0.0 60.14394 3 CAGCGTA 1020 0.0 59.71341 1 CGGGCCC 735 0.0 59.390594 7 TGTGTGG 5375 0.0 58.945053 6 >>END_MODULE