FastQCFastQC Report
Mon 6 Mar 2017
1NF_15A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_15A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2554324
Sequences flagged as poor quality0
Sequence length95
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA147470.5773347468841071No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA61500.2407682032506448No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT58400.22863191983475864No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG57770.22616551385023984No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA57120.2236208092630379No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG51950.2033806204694471No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG48920.19151838216295192No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG44560.17444928677802815No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT37410.14645753631880684No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA37140.1454005051825845No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT36760.1439128317316049No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA36560.14312984570477355No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA35090.137374898407563No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC33800.13232463853450072No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA32500.12723522936009685No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC31450.12312455271923217No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA30190.11819174075019458No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC29990.11740875472336319No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG27860.10906995353760916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTT301.9388608E-888.9997253
TGCTGCA301.9388608E-888.9997252
GCAGCTT158.5779617E-488.9997253
GCAGCTA202.4113197E-588.9997253
GCAGCAC301.9388608E-888.9997253
GCTGCAC450.088.999723
AGCTGCG500.088.999722
AGCAGCC450.088.999722
CAGCTGC4450.086.999731
CAGCAGC3350.086.343021
CTGCTGC1500.086.033071
AGCTGCT1000.084.5497362
AGCTGCC2000.084.5497362
AGCAGCG900.084.055292
AGCAGCA1650.083.6058042
CAGCCCT152250.083.446371
GCTGCCC1300.082.1535953
CAGCGTA50300.082.099151
AGCTGCA1100.080.908842
GCAGCAT550.080.908843
Produced by FastQC (version 0.11.5)