##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1NF_15A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2554324 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.829427277040814 12.0 12.0 12.0 2.0 32.0 2 14.598190362694787 12.0 12.0 12.0 12.0 22.0 3 13.71385462455037 12.0 12.0 12.0 12.0 22.0 4 13.760508846959118 12.0 12.0 12.0 12.0 22.0 5 22.422164533551733 22.0 22.0 22.0 22.0 27.0 6 26.77631929230591 27.0 22.0 32.0 22.0 32.0 7 34.91007679526951 37.0 37.0 37.0 27.0 41.0 8 37.53144158689344 41.0 37.0 41.0 27.0 41.0 9 38.49213686282555 41.0 37.0 41.0 32.0 41.0 10-11 38.86471175935394 41.0 41.0 41.0 37.0 41.0 12-13 39.040626795974205 41.0 41.0 41.0 37.0 41.0 14-15 38.98794553862392 41.0 41.0 41.0 37.0 41.0 16-17 38.889853244928986 41.0 41.0 41.0 37.0 41.0 18-19 38.82206975309319 41.0 41.0 41.0 37.0 41.0 20-21 38.8424681050642 41.0 41.0 41.0 37.0 41.0 22-23 38.81937451944233 41.0 41.0 41.0 34.5 41.0 24-25 38.83305641727517 41.0 41.0 41.0 34.5 41.0 26-27 38.804109815356234 41.0 41.0 41.0 37.0 41.0 28-29 38.75484081111088 41.0 41.0 41.0 32.0 41.0 30-31 38.62293702756581 41.0 41.0 41.0 32.0 41.0 32-33 38.66750380922702 41.0 41.0 41.0 32.0 41.0 34-35 38.64592588880659 41.0 41.0 41.0 32.0 41.0 36-37 38.56281329228399 41.0 41.0 41.0 32.0 41.0 38-39 38.46995173674131 41.0 41.0 41.0 32.0 41.0 40-41 38.5071408325647 41.0 41.0 41.0 32.0 41.0 42-43 38.24652315054786 41.0 41.0 41.0 32.0 41.0 44-45 38.36965063946469 41.0 41.0 41.0 32.0 41.0 46-47 38.35155230894749 41.0 41.0 41.0 32.0 41.0 48-49 38.3081040228256 41.0 41.0 41.0 32.0 41.0 50-51 38.204056728903616 41.0 41.0 41.0 32.0 41.0 52-53 38.164549798694296 41.0 39.0 41.0 32.0 41.0 54-55 38.03264405768415 41.0 37.0 41.0 32.0 41.0 56-57 37.80863469160529 41.0 37.0 41.0 27.0 41.0 58-59 37.801947990936156 41.0 37.0 41.0 27.0 41.0 60-61 37.80437113694269 41.0 37.0 41.0 27.0 41.0 62-63 37.79677773845448 41.0 37.0 41.0 27.0 41.0 64-65 37.78307841918253 41.0 37.0 41.0 27.0 41.0 66-67 37.65743578340101 41.0 37.0 41.0 27.0 41.0 68-69 37.688426174596486 41.0 37.0 41.0 27.0 41.0 70-71 37.661985127963405 41.0 37.0 41.0 27.0 41.0 72-73 37.49989429688638 41.0 37.0 41.0 27.0 41.0 74-75 37.415066373725494 41.0 37.0 41.0 27.0 41.0 76-77 36.37112872133684 39.0 34.5 41.0 24.5 41.0 78-79 36.87280313695521 41.0 37.0 41.0 27.0 41.0 80-81 37.548217454011315 41.0 37.0 41.0 27.0 41.0 82-83 37.72973142796294 41.0 37.0 41.0 27.0 41.0 84-85 37.650314916979994 41.0 37.0 41.0 27.0 41.0 86-87 37.513457768082674 41.0 37.0 41.0 27.0 41.0 88-89 37.40763192140073 41.0 37.0 41.0 27.0 41.0 90-91 37.24740988222324 41.0 37.0 41.0 27.0 41.0 92-93 37.128056190209236 41.0 37.0 41.0 27.0 41.0 94-95 36.96230529094977 41.0 37.0 41.0 27.0 41.0 96-97 36.90866565870265 41.0 37.0 41.0 27.0 41.0 98-99 36.78577306559387 41.0 37.0 41.0 27.0 41.0 100 36.63667060247643 41.0 37.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.5277273354487306 1101 2 -0.6966472252574931 1101 3 -0.018442927339357595 1101 4 0.07326230680946644 1101 5 -1.0687777202598028 1101 6 -0.5232216854085046 1101 7 -0.10805111086140329 1101 8 0.04305132220940777 1101 9 0.17773866410340133 1101 10-11 0.043739413232536606 1101 12-13 0.09500633862756303 1101 14-15 0.13182611696372248 1101 16-17 0.0040062670125209365 1101 18-19 0.09932937228150251 1101 20-21 0.14160143914373435 1101 22-23 0.03826713198780851 1101 24-25 0.16414606824653788 1101 26-27 0.13104744781846023 1101 28-29 0.10337465921614353 1101 30-31 0.21413002055438568 1101 32-33 0.048076141338704304 1101 34-35 0.17661531514013973 1101 36-37 0.2216920896258756 1101 38-39 0.42911699251719426 1101 40-41 0.20420954560844962 1101 42-43 0.3451708134145193 1101 44-45 0.19676201784842107 1101 46-47 0.2655192199825436 1101 48-49 0.15581659555364524 1101 50-51 0.2460764377910465 1101 52-53 0.32308968951711137 1101 54-55 0.11482006244982301 1101 56-57 0.36037043157446647 1101 58-59 0.13907541377161436 1101 60-61 0.12143041743404837 1101 62-63 0.08769016715155686 1101 64-65 0.16405558251337027 1101 66-67 0.15265038393937402 1101 68-69 0.10825531866021265 1101 70-71 -0.009855479982412874 1101 72-73 0.17173252263246752 1101 74-75 0.307212605954831 1101 76-77 -0.0725613872969646 1101 78-79 0.16187365027450085 1101 80-81 0.12467402339544975 1101 82-83 0.1709705609281329 1101 84-85 0.2817529297071104 1101 86-87 0.2245777443857051 1101 88-89 -0.1357620607317358 1101 90-91 -0.11985233858547417 1101 92-93 0.22898640894801048 1101 94-95 0.32121428855742806 1101 96-97 0.183734866709095 1101 98-99 0.011329999361372245 1101 100 -0.2674776522783304 1102 1 1.60268716474382 1102 2 0.6555567329194911 1102 3 0.06246408757536592 1102 4 0.15988222724441492 1102 5 -0.7252280101397659 1102 6 0.10451292209489083 1102 7 0.19264788685108414 1102 8 -0.08691472972196124 1102 9 0.07939905323970464 1102 10-11 0.07256498179798854 1102 12-13 -0.10163759404720452 1102 14-15 -0.05314468722673382 1102 16-17 -0.1966500309462802 1102 18-19 -0.09439282288577999 1102 20-21 -0.025582114118826382 1102 22-23 -0.09416199779199275 1102 24-25 0.011630115634247318 1102 26-27 -0.028248512055526476 1102 28-29 -0.13341057082534746 1102 30-31 -0.1271025711599023 1102 32-33 -0.05215702127403432 1102 34-35 0.06843544997568785 1102 36-37 -0.2065001776800699 1102 38-39 -0.2079256907682634 1102 40-41 -0.18718594429377333 1102 42-43 0.014707754386911631 1102 44-45 -0.00802937287745209 1102 46-47 -0.36879963856560494 1102 48-49 -0.14993442214341712 1102 50-51 -0.0337331071919067 1102 52-53 -0.09553336301730297 1102 54-55 -0.1383592851418598 1102 56-57 -3.725108756214013E-5 1102 58-59 -0.0849855641429258 1102 60-61 -0.0053936344863458885 1102 62-63 -0.18218060825871163 1102 64-65 -0.06858837917683758 1102 66-67 0.0055308136502461025 1102 68-69 -0.2769172408861422 1102 70-71 -0.0803297766127784 1102 72-73 -0.07066925421053583 1102 74-75 -0.09835928059007415 1102 76-77 0.006638813772219976 1102 78-79 -0.08276280582630591 1102 80-81 -0.188072460834654 1102 82-83 -0.19340527767699456 1102 84-85 -0.24272894858015803 1102 86-87 -0.2826756263069754 1102 88-89 -0.12855221320396737 1102 90-91 -0.28311949318211305 1102 92-93 -0.2693638278415307 1102 94-95 -0.23532612564194721 1102 96-97 -0.07714239561325087 1102 98-99 -0.29800873868642697 1102 100 -0.477164614855468 1103 1 1.7490056848677842 1103 2 0.7629817678723381 1103 3 0.23184752604992376 1103 4 1.0050708202762717 1103 5 2.8593274117816776 1103 6 -0.3405563143009225 1103 7 -0.42349215285185693 1103 8 0.07983335647887913 1103 9 -0.09756361287391968 1103 10-11 -0.22393946939609322 1103 12-13 -0.3211996713807963 1103 14-15 -0.3371563011393022 1103 16-17 -0.2444562636945662 1103 18-19 -0.19185901312798137 1103 20-21 -0.08612016148406809 1103 22-23 -0.19914759516728253 1103 24-25 -0.2687158926723754 1103 26-27 -0.20299771788499044 1103 28-29 -0.2346881093323816 1103 30-31 -0.04635027091418209 1103 32-33 -0.2575675372111732 1103 34-35 -0.25997973724218326 1103 36-37 -0.26421467685040767 1103 38-39 -0.24840964983356884 1103 40-41 -0.278806685942115 1103 42-43 -0.0018201740706089709 1103 44-45 -0.4855488470420042 1103 46-47 -0.3685378095937466 1103 48-49 -0.31017578417407776 1103 50-51 -0.14007781833808508 1103 52-53 -0.149925538942 1103 54-55 -0.27422150582123095 1103 56-57 -0.34528688337933744 1103 58-59 -0.4099409120453785 1103 60-61 -0.17509902712721725 1103 62-63 -0.333079002570706 1103 64-65 -0.44808611442731916 1103 66-67 -0.2927676433463233 1103 68-69 -0.34839380800566744 1103 70-71 -0.42237835167613724 1103 72-73 -0.33174818457521127 1103 74-75 -0.4389431165409263 1103 76-77 -0.5178000465222183 1103 78-79 -0.4430241351090416 1103 80-81 -0.36480640745850934 1103 82-83 -0.48834322380441364 1103 84-85 -0.3792367025974315 1103 86-87 -0.1941117226706055 1103 88-89 -0.3218364321076166 1103 90-91 -0.49280174168563917 1103 92-93 -0.3658510539799025 1103 94-95 -0.43497315418780147 1103 96-97 -0.2977593044835629 1103 98-99 -0.315484045208116 1103 100 -0.2680136415077854 1104 1 1.4792730498742337 1104 2 -0.4966036577546564 1104 3 0.02777182550243218 1104 4 0.08845559974384187 1104 5 -1.1581872058603935 1104 6 -0.43152374100250057 1104 7 -0.26190904818477634 1104 8 -0.17742004555390167 1104 9 -0.06621838346438835 1104 10-11 0.0264623938088846 1104 12-13 -0.026937237961547567 1104 14-15 -0.16359213106350978 1104 16-17 -0.03616087377108812 1104 18-19 -0.09719792497234181 1104 20-21 0.11914408054590098 1104 22-23 0.12077192557803329 1104 24-25 -0.04457197545609404 1104 26-27 -0.03465118155255453 1104 28-29 0.0978262095902025 1104 30-31 -3.2926479878625514E-4 1104 32-33 8.971371473691647E-4 1104 34-35 -0.16507534860028272 1104 36-37 -0.2099977936271955 1104 38-39 -0.19163604963611647 1104 40-41 -0.23025601859070832 1104 42-43 -0.2412591326037159 1104 44-45 -0.16463158308722825 1104 46-47 -0.2916721555157409 1104 48-49 -0.37323617238067186 1104 50-51 -0.18077296775716434 1104 52-53 -0.04393040977951301 1104 54-55 -0.1396887081311604 1104 56-57 -0.1403092913641828 1104 58-59 -0.16843485487911636 1104 60-61 -0.3095718797615561 1104 62-63 -0.4038086364894724 1104 64-65 -0.36847938215501586 1104 66-67 -0.6298100267134643 1104 68-69 -0.5282076524570982 1104 70-71 -0.26743989493142806 1104 72-73 -0.2384511481742706 1104 74-75 -0.31836635257216983 1104 76-77 -0.29510104653851243 1104 78-79 -0.42536910924918914 1104 80-81 -0.49905768466033607 1104 82-83 -0.16993087866330825 1104 84-85 -0.21046753350631064 1104 86-87 -0.41203171372310265 1104 88-89 -0.2164857122942223 1104 90-91 -0.30671498912052186 1104 92-93 -0.4713920691715501 1104 94-95 -0.27008613150785266 1104 96-97 -0.24641093298755834 1104 98-99 -0.33165341935960413 1104 100 -0.29749554524206445 1105 1 1.6698424470760376 1105 2 0.3559617135303075 1105 3 -0.05637792970525268 1105 4 -0.19951482572376378 1105 5 -1.1893309099186844 1105 6 0.4214485946276305 1105 7 0.0319723330238304 1105 8 -0.26695642072516534 1105 9 -0.12535958513306156 1105 10-11 -0.23632898268952118 1105 12-13 -0.22622597091775987 1105 14-15 -0.0328886849462009 1105 16-17 -0.10460989851421232 1105 18-19 -0.15840549673384885 1105 20-21 -0.07573574414873718 1105 22-23 -0.18497403891596065 1105 24-25 -0.22347921673647875 1105 26-27 -0.11161289545163555 1105 28-29 -0.3061513011228243 1105 30-31 -0.2027597931850309 1105 32-33 -0.3027540733126486 1105 34-35 -0.10916080650996207 1105 36-37 -0.3288735747609266 1105 38-39 -0.21482093495731647 1105 40-41 -0.16655066000947727 1105 42-43 -0.0377280552567143 1105 44-45 -0.08616427371607926 1105 46-47 -0.3109449474369086 1105 48-49 -0.3447506819445252 1105 50-51 -0.08987013596039617 1105 52-53 -0.2132886603774935 1105 54-55 -0.27763111404607344 1105 56-57 -0.27772803274351077 1105 58-59 -0.23054754833101754 1105 60-61 -0.2861006618449906 1105 62-63 -0.3744796827215495 1105 64-65 -0.3060857260425749 1105 66-67 -0.24936654297592753 1105 68-69 -0.1593706115673399 1105 70-71 -0.20058976827767339 1105 72-73 -0.27792764857242247 1105 74-75 -0.33104448098112016 1105 76-77 -0.16088192328009399 1105 78-79 -0.239998096617569 1105 80-81 -0.43199726752380485 1105 82-83 -0.24761839678684083 1105 84-85 -0.31186130008597956 1105 86-87 -0.3280822762621298 1105 88-89 -0.21184445846047595 1105 90-91 -0.5629740317071708 1105 92-93 -0.3012352325541201 1105 94-95 -0.2590863954017948 1105 96-97 -0.2818010088877543 1105 98-99 -0.45428115476604347 1105 100 -0.47606908343073684 1106 1 1.4268957924358467 1106 2 -0.6040082813263883 1106 3 -0.21565338790943223 1106 4 -0.14245392566103732 1106 5 -1.1674394412386526 1106 6 -0.5568484227146087 1106 7 -0.1177124679822441 1106 8 -0.17747096467293488 1106 9 -0.008039818214719219 1106 10-11 -0.043505683375244075 1106 12-13 -7.752260545714762E-4 1106 14-15 -0.11454891866887351 1106 16-17 -0.09196302059057615 1106 18-19 -0.004729962066917892 1106 20-21 0.016983258294708037 1106 22-23 0.1680708688327215 1106 24-25 -0.043034565237206834 1106 26-27 -0.06080885376188405 1106 28-29 0.031100175859755552 1106 30-31 0.04103094688588271 1106 32-33 -0.11671474459760844 1106 34-35 -0.17485095151447183 1106 36-37 -0.13938483737307905 1106 38-39 0.05870490594216449 1106 40-41 -0.07474642525509267 1106 42-43 0.11700084537375943 1106 44-45 -0.2509490371775698 1106 46-47 -0.289631051056908 1106 48-49 -0.37457945345826005 1106 50-51 -0.24401335633947951 1106 52-53 -0.2625490435153992 1106 54-55 -0.1243157631949856 1106 56-57 -0.030220130829505365 1106 58-59 -0.30454223867214125 1106 60-61 -0.2771815337950656 1106 62-63 -0.2578941146460423 1106 64-65 -0.48571862852890746 1106 66-67 -0.42305612633787604 1106 68-69 -0.28532200811903863 1106 70-71 -0.29735921705908197 1106 72-73 -0.1373500800878915 1106 74-75 -0.6312232565392861 1106 76-77 -0.44747940336696956 1106 78-79 -0.31628733719441016 1106 80-81 -0.21215188329467338 1106 82-83 -0.3822207866675029 1106 84-85 -0.40403916831670017 1106 86-87 -0.5503676769132042 1106 88-89 -0.49674193961568847 1106 90-91 -0.5691845316758872 1106 92-93 -0.3237063720627731 1106 94-95 -0.33941482657234445 1106 96-97 -0.16462499117769624 1106 98-99 -0.5053483710182718 1106 100 -0.5503743560028909 1107 1 1.4528814109368824 1107 2 -0.5524565290994623 1107 3 -0.17938356359550944 1107 4 -0.21977277408753082 1107 5 -1.2981577259946704 1107 6 -0.30149321178693356 1107 7 -0.44458241892203887 1107 8 -0.19215566333165413 1107 9 -0.0732474583006848 1107 10-11 -0.3070504077688625 1107 12-13 -0.28458106508753644 1107 14-15 -0.3258455461088374 1107 16-17 -0.23258612132448775 1107 18-19 -0.3044255917184415 1107 20-21 -0.30182614635884164 1107 22-23 -0.21867019297104662 1107 24-25 -0.1935421905924244 1107 26-27 -0.20615574328228092 1107 28-29 -0.10578463264544524 1107 30-31 -0.35270712096724566 1107 32-33 -0.14794450811550774 1107 34-35 -0.3726009522461311 1107 36-37 -0.20117982248137167 1107 38-39 -0.2876174320123326 1107 40-41 -0.4770169109891924 1107 42-43 -0.2447333632194315 1107 44-45 -0.09022061649871915 1107 46-47 -0.35856732546628933 1107 48-49 -0.3012712697005142 1107 50-51 -0.3501188109097626 1107 52-53 -0.2695516101768973 1107 54-55 -0.1552234989820107 1107 56-57 -0.29612489607899306 1107 58-59 -0.46876662595609986 1107 60-61 -0.29393068287507873 1107 62-63 -0.24787672066106126 1107 64-65 -0.49713241904211714 1107 66-67 -0.41175130431160767 1107 68-69 -0.6207171578994917 1107 70-71 -0.5207082274164563 1107 72-73 -0.3048638133365458 1107 74-75 -0.42517555429152765 1107 76-77 -0.400933470271859 1107 78-79 -0.6579554427914047 1107 80-81 -0.655819175286311 1107 82-83 -0.41718016005480507 1107 84-85 -0.3246091408160581 1107 86-87 -0.34817328376622214 1107 88-89 -0.42114610051443435 1107 90-91 -0.20635615070176527 1107 92-93 -0.4722950984582184 1107 94-95 -0.4750151336742192 1107 96-97 -0.3352992007659523 1107 98-99 -0.4815351958794807 1107 100 -0.5213863971670278 1108 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0.3052343255909946 2223 24-25 0.3775580633558775 2223 26-27 0.3652393522557702 2223 28-29 0.33973057399742146 2223 30-31 0.3513607365172007 2223 32-33 0.37980134448517333 2223 34-35 0.30103644237312466 2223 36-37 0.2838965669632785 2223 38-39 0.497591689826244 2223 40-41 0.5130909145614027 2223 42-43 0.5718062642319737 2223 44-45 0.6454695626704918 2223 46-47 0.5508913215611884 2223 48-49 0.3350886224739327 2223 50-51 0.33222163206508526 2223 52-53 0.4168616677471704 2223 54-55 0.5213564556937769 2223 56-57 0.6022472777457892 2223 58-59 0.7189057553606446 2223 60-61 0.5852359919246979 2223 62-63 0.5769228631838459 2223 64-65 0.4269950118638448 2223 66-67 0.45829655742808484 2223 68-69 0.5971972258025318 2223 70-71 0.6151607500951641 2223 72-73 0.6147825329115193 2223 74-75 0.7790710039379718 2223 76-77 0.7566927865379895 2223 78-79 0.6979828678765614 2223 80-81 0.6609103766052229 2223 82-83 0.47989250507502845 2223 84-85 0.4755657536324165 2223 86-87 0.6349836586259698 2223 88-89 0.45129201788675744 2223 90-91 0.5161232513778415 2223 92-93 0.6884761353089033 2223 94-95 0.7500332258119471 2223 96-97 0.5416729976533645 2223 98-99 0.6753214082403005 2223 100 0.6968242920849619 2224 1 1.1534254056646347 2224 2 -0.8973081932015958 2224 3 -0.04928673900659497 2224 4 -0.3562824011368271 2224 5 -1.1097503938832922 2224 6 0.6424524809261669 2224 7 0.3470737787637077 2224 8 0.28801368408061734 2224 9 0.17772701503847088 2224 10-11 0.24376754572433867 2224 12-13 0.2612617933022179 2224 14-15 0.22478303406198563 2224 16-17 0.26656920108580806 2224 18-19 0.2475841093737614 2224 20-21 0.08788032008795454 2224 22-23 0.19893132672289937 2224 24-25 0.2865596846055496 2224 26-27 0.22558659916078483 2224 28-29 0.27307940201644243 2224 30-31 0.2603488505688958 2224 32-33 0.2824911485960726 2224 34-35 0.21713513136448626 2224 36-37 0.07666006634638478 2224 38-39 0.20620631055763283 2224 40-41 0.2644599655572506 2224 42-43 0.40471180530909834 2224 44-45 0.41484882202065165 2224 46-47 0.24619662475726045 2224 48-49 0.18070482277565247 2224 50-51 0.2626440291604908 2224 52-53 0.32859270778983785 2224 54-55 0.43582868508768513 2224 56-57 0.4105493612117783 2224 58-59 0.4508958364462856 2224 60-61 0.31306132147138754 2224 62-63 0.48016318209264597 2224 64-65 0.320644351882855 2224 66-67 0.22531987200956394 2224 68-69 0.3220785650682245 2224 70-71 0.5828463225938947 2224 72-73 0.5616369187565979 2224 74-75 0.33553060911872024 2224 76-77 0.5659733700180709 2224 78-79 0.379562638879392 2224 80-81 0.28605900402305195 2224 82-83 0.3309497906453629 2224 84-85 0.35998601118766516 2224 86-87 0.36097577196602515 2224 88-89 0.3183007253984229 2224 90-91 0.45550429753733823 2224 92-93 0.37096812457569683 2224 94-95 0.5266994255154813 2224 96-97 0.2573319115743047 2224 98-99 0.4116314771617411 2224 100 0.6184005357795144 2225 1 1.2651559902002205 2225 2 1.8649422982742898 2225 3 0.8869473710955607 2225 4 1.0343400887533907 2225 5 3.1292565532337875 2225 6 -0.13368704390535768 2225 7 0.17783329247706803 2225 8 0.07798445556773714 2225 9 0.1291127978922333 2225 10-11 -0.16226087839767445 2225 12-13 0.06368386504787082 2225 14-15 -0.023847132213461464 2225 16-17 0.06795313476516185 2225 18-19 -0.10140595246956252 2225 20-21 -0.10140117164103657 2225 22-23 -0.006260288195782948 2225 24-25 -0.006235411846233774 2225 26-27 -0.08103020914108328 2225 28-29 -0.019373886104574467 2225 30-31 -0.03203018164256832 2225 32-33 -0.05663125634769273 2225 34-35 0.03187472715808326 2225 36-37 -0.029273067507261885 2225 38-39 -0.029054902662579707 2225 40-41 0.08324376355343333 2225 42-43 0.09880403981677688 2225 44-45 0.07674924166766317 2225 46-47 0.02895330461085166 2225 48-49 -0.006420069307694121 2225 50-51 -0.10945724549853963 2225 52-53 0.13213786405085415 2225 54-55 -0.07456598651627644 2225 56-57 0.06498027201106993 2225 58-59 0.18013625338888772 2225 60-61 0.15430998804328055 2225 62-63 0.015003072482834057 2225 64-65 -0.06880593479696984 2225 66-67 0.15973456786082352 2225 68-69 0.11615363692598635 2225 70-71 0.2004046958289436 2225 72-73 0.013524868033734094 2225 74-75 -0.11520160615754094 2225 76-77 0.26762872782753533 2225 78-79 0.21796733300860893 2225 80-81 0.14043594204451182 2225 82-83 0.08977064147362057 2225 84-85 0.11290077233789475 2225 86-87 0.137333441590215 2225 88-89 0.17594376770637865 2225 90-91 0.24554990280101663 2225 92-93 0.20652046719072814 2225 94-95 0.3356239456836221 2225 96-97 0.12963746221799255 2225 98-99 0.3173264720245115 2225 100 0.3602459656027719 2226 1 1.4702911573228974 2226 2 1.4945119773959625 2226 3 0.8967173700048487 2226 4 1.1943905400066654 2226 5 3.2066855350885106 2226 6 -0.3050484155110951 2226 7 0.1297131470177959 2226 8 0.28150443997287056 2226 9 0.1446055562576518 2226 10-11 0.2148146662043402 2226 12-13 0.31201240620833914 2226 14-15 0.18898335946579436 2226 16-17 0.20247627295379544 2226 18-19 0.21521221230227638 2226 20-21 0.2731228019869292 2226 22-23 0.14249490719259228 2226 24-25 0.2956656767776451 2226 26-27 0.22083664967662742 2226 28-29 0.3271593887480009 2226 30-31 0.257487259313919 2226 32-33 0.05571850961324998 2226 34-35 0.1998067363200704 2226 36-37 0.28280413637011037 2226 38-39 0.28652650509911837 2226 40-41 0.29476772093674697 2226 42-43 0.45660530272257205 2226 44-45 0.3112402386484874 2226 46-47 0.2744773223314496 2226 48-49 0.36866260437666654 2226 50-51 0.21811445724286216 2226 52-53 0.24970564486870472 2226 54-55 0.4572068157561091 2226 56-57 0.456341829269995 2226 58-59 0.3478338754036443 2226 60-61 0.3904898488498034 2226 62-63 0.39466963936092014 2226 64-65 0.46789015789966726 2226 66-67 0.4740187239858713 2226 68-69 0.5445129733086702 2226 70-71 0.5533401047648923 2226 72-73 0.5103948713522826 2226 74-75 0.6570709744913614 2226 76-77 0.7810085208706496 2226 78-79 0.6146838243490507 2226 80-81 0.4540238987223404 2226 82-83 0.4476612499751411 2226 84-85 0.4694918085379456 2226 86-87 0.48584218237427734 2226 88-89 0.35877676570364514 2226 90-91 0.4554041418434167 2226 92-93 0.3930531850837582 2226 94-95 0.5531711886177746 2226 96-97 0.6283512337392594 2226 98-99 0.5707158030000485 2226 100 0.5235662335931437 2227 1 1.2191691402356533 2227 2 1.5921295152058015 2227 3 0.5751984751472357 2227 4 -0.02432494334075308 2227 5 3.2072930867802185 2227 6 0.05753687126697926 2227 7 0.0616435243288862 2227 8 0.22370419906201988 2227 9 0.1880689447238595 2227 10-11 0.21787117867008732 2227 12-13 0.07411508986769633 2227 14-15 0.1559790417463347 2227 16-17 0.2346879408375102 2227 18-19 0.2565053128202379 2227 20-21 0.05543312376271814 2227 22-23 0.17365644835002314 2227 24-25 0.22821933179689324 2227 26-27 0.19254515536124472 2227 28-29 0.14881620370026383 2227 30-31 0.14617968695372952 2227 32-33 0.06467911564899964 2227 34-35 0.2703548182735176 2227 36-37 0.2097372545012064 2227 38-39 0.14920106876564887 2227 40-41 0.16802696812745666 2227 42-43 0.3011896728985377 2227 44-45 0.324192322550779 2227 46-47 0.16571189471859782 2227 48-49 0.15226587820119164 2227 50-51 0.21947089942015907 2227 52-53 0.27072751946456464 2227 54-55 0.40528214669596707 2227 56-57 0.3945755065440082 2227 58-59 0.5262869472123484 2227 60-61 0.40188867117629457 2227 62-63 0.2036820533415593 2227 64-65 0.2861552325074044 2227 66-67 0.48478147290136064 2227 68-69 0.4562501687172187 2227 70-71 0.3881959313809702 2227 72-73 0.3484353628166801 2227 74-75 0.3424091111947902 2227 76-77 0.4150564990909942 2227 78-79 0.4282076515105615 2227 80-81 0.21628892175655778 2227 82-83 0.13317940561231012 2227 84-85 0.23169597872587389 2227 86-87 0.23661197576372217 2227 88-89 0.22277631951455135 2227 90-91 0.30308556049028823 2227 92-93 0.2218126895560033 2227 94-95 0.5010222528881485 2227 96-97 0.2918222957200811 2227 98-99 0.4373245080631989 2227 100 0.10324751705488211 2228 1 1.58445510853306 2228 2 1.5245924253056842 2228 3 -0.1471016235373579 2228 4 -0.049531485932401864 2228 5 3.1363132219939835 2228 6 0.26310160394377746 2228 7 0.189411870035876 2228 8 0.10569513063426683 2228 9 -0.11566066751892379 2228 10-11 0.12694253197221883 2228 12-13 0.1336395299363602 2228 14-15 0.14041140838111232 2228 16-17 0.14832448438713897 2228 18-19 0.1528444222266785 2228 20-21 0.11368734147266935 2228 22-23 0.1372633253334925 2228 24-25 0.2773087508236074 2228 26-27 0.09744464182275436 2228 28-29 0.12640613890420127 2228 30-31 0.1683211942753431 2228 32-33 -0.03250532358214997 2228 34-35 0.17335289104618568 2228 36-37 0.2219355384485837 2228 38-39 0.2215738185092846 2228 40-41 0.1284319975425774 2228 42-43 0.26985803128290087 2228 44-45 0.22890260458297718 2228 46-47 0.09872138476916348 2228 48-49 0.17693444538922165 2228 50-51 0.1520875440942433 2228 52-53 0.13181408637074554 2228 54-55 0.06937869889219428 2228 56-57 0.3561331628413029 2228 58-59 0.40046294232343627 2228 60-61 0.23621949459511882 2228 62-63 0.37193469737545826 2228 64-65 0.2952200272325527 2228 66-67 0.301127741290955 2228 68-69 0.349922920200882 2228 70-71 0.2650052047116773 2228 72-73 0.3908777692369725 2228 74-75 0.42322846676010784 2228 76-77 0.37866242001367567 2228 78-79 0.3705110084503076 2228 80-81 0.4186632795944334 2228 82-83 0.26204970697988017 2228 84-85 0.26721306324527205 2228 86-87 0.2453013254186729 2228 88-89 0.25030360405526153 2228 90-91 0.3056202688429863 2228 92-93 0.41324148989284026 2228 94-95 0.5712849764796957 2228 96-97 0.42684350975283536 2228 98-99 0.5645018169930154 2228 100 0.5526092795567621 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 23.0 13 25.0 14 137.0 15 447.0 16 1744.0 17 4366.0 18 7112.0 19 9095.0 20 10795.0 21 12411.0 22 14313.0 23 16330.0 24 18593.0 25 20741.0 26 23468.0 27 26424.0 28 30133.0 29 34186.0 30 39330.0 31 45217.0 32 53191.0 33 63025.0 34 76848.0 35 99214.0 36 138926.0 37 229649.0 38 560075.0 39 1017308.0 40 1196.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.926797625744825 18.600775580603596 20.756804876390188 34.715621917261394 2 18.98442767171493 52.6783120463748 10.533031392880439 17.804228889029837 3 41.440122709570126 15.34312013667804 23.56455171701006 19.652205436741777 4 17.433927724125837 15.995934736548692 22.226115402744522 44.34402213658095 5 17.159960913337542 35.50438393876423 26.404833529340838 20.930821618557395 6 17.117053279067182 27.861265837849857 31.25183022983772 23.769850653245243 7 20.15398203203666 26.667133848329343 36.73743033381826 16.44145378581574 8 20.198377339757993 27.878961322056245 34.79609477889258 17.12656655929318 9 20.661865918340823 27.96297572273525 27.8292808586538 23.54587750027013 10-11 19.231585343127968 30.294708110639057 28.928143023359603 21.54556352287337 12-13 23.973094251159992 26.423507746080766 27.549108883602862 22.05428911915638 14-15 21.839927119660622 27.46865706934594 28.511614031735988 22.179801779257446 16-17 20.82862236740523 29.81295638298039 28.30320272604415 21.05521852357023 18-19 21.41697764261699 29.534702723695194 29.82797014004488 19.220349493642935 20-21 22.646559324502295 29.026857986692367 29.97399703404893 18.352585654756407 22-23 22.042524754103237 30.261548652402748 28.69281657299544 19.003110020498575 24-25 22.291142392272867 28.424174067189593 26.38878231579079 22.895901224746744 26-27 20.06468247567654 26.40622332953846 30.170585250735616 23.358508944049383 28-29 19.356066864031806 28.178558816874016 30.86945321608351 21.595921103010667 30-31 19.945400923427194 30.11230104078878 28.297963350687972 21.644334685096055 32-33 20.444274101484385 30.162285598851202 28.925343848313684 20.46809645135073 34-35 21.312566455939027 29.270836432653024 30.072888169237732 19.343708942170217 36-37 22.002240123022766 28.18326884138426 28.71638445240306 21.098106583189917 38-39 21.601848957318907 29.220556906978008 29.032932447952074 20.144661687751007 40-41 20.73020768613707 30.042356242661842 29.780815546569357 19.446620524631726 42-43 20.41442316482087 30.05692509733015 29.388998213419885 20.13965352442909 44-45 20.543909073398677 29.96119521251024 28.978939241850288 20.515956472240795 46-47 19.825148104590106 28.149972829874297 28.920386833685946 23.10449223184965 48-49 20.759293018288403 28.042571741598117 30.31538487587809 20.882750364235385 50-51 21.900373640932003 30.557028004278237 28.261489145464708 19.281109209325052 52-53 20.47189685419062 30.322923774814655 28.37678381481799 20.828395556176734 54-55 22.468435036839903 28.32391704898602 28.240571332245164 20.967076581928918 56-57 21.646556689228316 28.88168504866214 29.363705879197617 20.10805238291193 58-59 21.207487831011846 28.034690059106932 29.017684702070007 21.740137407811215 60-61 19.48896710940733 30.50835115610161 29.67938200309515 20.32329973139591 62-63 20.43360799675807 31.43306571758022 27.31201804203936 20.821308243622347 64-65 19.942552270230614 30.138604294994913 28.95930896731109 20.95953446746338 66-67 20.08641033792111 30.296626426404792 29.53671891271428 20.08024432295981 68-69 20.800571892993997 29.975445558198565 29.309183173317088 19.914799375490343 70-71 19.598492595301146 31.036978864075195 29.250165601544676 20.11436293907899 72-73 20.02322336555582 30.66435581390615 28.800085658671335 20.5123351618667 74-75 19.911647856732348 29.17486192041417 30.67917382446393 20.234316398389556 76-77 19.44434222126872 30.459879012999135 28.757863137174454 21.337915628557695 78-79 19.49703718087447 29.519767265233384 28.557144669196234 22.42605088469591 80-81 20.49492624260077 29.62451846283943 28.597219612264713 21.283335682295093 82-83 20.068440808605327 28.559297880770018 30.05069051537706 21.321570795247588 84-85 20.33708331441117 29.336803005413564 28.323756109248478 22.00235757092679 86-87 21.711478261958934 28.542639853049184 29.22787007443065 20.51801181056123 88-89 22.384161132260434 27.894112101675432 29.05326419044726 20.668462575616875 90-91 23.10705298153249 28.131709211517414 28.564857081560525 20.196380725389577 92-93 22.39107098394722 28.565091977368574 29.677362777783866 19.36647426090034 94-95 22.310051504820844 29.28770978153124 28.725897732629065 19.676340981018853 96-97 22.167587197238877 28.628513845541914 29.030068229402378 20.17383072781683 98-99 23.125159533402968 29.01587660766606 28.51637067184899 19.342593187081984 100 23.35909618356951 30.495778922329354 27.10247407924758 19.04265081485356 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2.0 1 5.5 2 9.0 3 14.0 4 25.5 5 32.0 6 31.5 7 37.5 8 53.5 9 65.5 10 66.5 11 82.5 12 108.0 13 111.5 14 118.0 15 166.5 16 247.0 17 334.5 18 460.5 19 686.5 20 1131.5 21 1869.0 22 2890.0 23 4304.5 24 6589.0 25 9843.0 26 14268.0 27 19381.0 28 24593.0 29 30530.5 30 37616.0 31 45738.0 32 54572.5 33 64639.5 34 75856.5 35 87905.0 36 100578.0 37 110352.5 38 116287.5 39 120872.5 40 123158.0 41 123247.0 42 121948.5 43 120101.5 44 119374.5 45 116691.5 46 107897.5 47 97574.0 48 92231.0 49 89417.5 50 85173.0 51 77932.0 52 67895.0 53 57596.5 54 48516.5 55 39776.5 56 30920.5 57 22793.5 58 16072.5 59 11461.0 60 8816.5 61 7232.0 62 6062.0 63 4938.0 64 4001.0 65 3611.5 66 3179.5 67 2350.0 68 1760.0 69 1884.5 70 2410.5 71 2306.0 72 1415.0 73 633.0 74 298.0 75 203.5 76 147.0 77 105.5 78 83.5 79 60.5 80 48.0 81 40.0 82 31.5 83 24.0 84 18.0 85 17.5 86 19.0 87 16.0 88 15.0 89 15.5 90 12.0 91 10.5 92 13.5 93 14.0 94 10.0 95 12.0 96 14.0 97 14.5 98 34.5 99 80.5 100 84.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.34147430004964 2 0.00696857563879915 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.3489580804940952E-4 30-31 0.009846049287404417 32-33 0.0 34-35 0.0 36-37 0.0 38-39 7.63411376160581E-4 40-41 0.0014485241496380256 42-43 9.787325335392064E-5 44-45 0.0 46-47 0.0018791664643952762 48-49 1.9574650670784128E-5 50-51 0.0 52-53 0.0014485241496380256 54-55 0.01971167322547962 56-57 0.011157550882346955 58-59 0.01540525007790711 60-61 2.7404510939097777E-4 62-63 0.011314148087713227 64-65 7.829860268313651E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.5659720536627302E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2554324.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.49395691363002 #Duplication Level Percentage of deduplicated Percentage of total 1 86.15443970380787 64.17985119213398 2 8.639734035571058 12.872159499821064 3 2.360628241187789 5.2755761546442415 4 1.0016081536178083 2.9845501855978207 5 0.5461429108762473 2.0342173235749823 6 0.32041478970275894 1.432137932316429 7 0.21395046617050212 1.1156611765989508 8 0.14577120740997412 0.868725923523715 9 0.10850849329513768 0.7274904321861808 >10 0.47718153479090664 6.0372227130588385 >50 0.02232948296929871 1.1213947556281318 >100 0.008969982724363198 1.1809955329527075 >500 3.209978762656885E-4 0.17001717796302898 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.9149301341568256E-5 0.0 3 0.0 0.0 0.0 3.9149301341568256E-5 0.0 4 0.0 0.0 0.0 3.9149301341568256E-5 0.0 5 0.0 0.0 0.0 3.9149301341568256E-5 0.0 6 0.0 0.0 0.0 3.9149301341568256E-5 0.0 7 0.0 0.0 0.0 3.9149301341568256E-5 0.0 8 0.0 0.0 0.0 3.9149301341568256E-5 0.0 9 0.0 0.0 0.0 3.9149301341568256E-5 0.0 10-11 0.0 0.0 0.0 3.9149301341568256E-5 0.0 12-13 0.0 0.0 0.0 3.9149301341568256E-5 0.0 14-15 0.0 0.0 0.0 3.9149301341568256E-5 0.0 16-17 0.0 0.0 0.0 3.9149301341568256E-5 0.0 18-19 0.0 0.0 0.0 3.9149301341568256E-5 0.0 20-21 0.0 0.0 0.0 3.9149301341568256E-5 0.0 22-23 0.0 0.0 0.0 3.9149301341568256E-5 0.0 24-25 0.0 0.0 0.0 3.9149301341568256E-5 0.0 26-27 0.0 0.0 0.0 3.9149301341568256E-5 0.0 28-29 0.0 0.0 0.0 3.9149301341568256E-5 0.0 30-31 0.0 0.0 0.0 3.9149301341568256E-5 0.0 32-33 0.0 0.0 0.0 3.9149301341568256E-5 0.0 34-35 0.0 0.0 0.0 3.9149301341568256E-5 0.0 36-37 0.0 0.0 1.9574650670784128E-5 3.9149301341568256E-5 0.0 38-39 0.0 0.0 3.9149301341568256E-5 3.9149301341568256E-5 0.0 40-41 0.0 0.0 3.9149301341568256E-5 3.9149301341568256E-5 0.0 42-43 0.0 0.0 3.9149301341568256E-5 3.9149301341568256E-5 0.0 44-45 0.0 0.0 3.9149301341568256E-5 3.9149301341568256E-5 0.0 46-47 0.0 0.0 3.9149301341568256E-5 3.9149301341568256E-5 0.0 48-49 1.9574650670784128E-5 0.0 3.9149301341568256E-5 3.9149301341568256E-5 0.0 50-51 7.829860268313651E-5 0.0 3.9149301341568256E-5 1.1744790402470477E-4 0.0 52-53 1.7617185603705716E-4 0.0 3.9149301341568256E-5 1.1744790402470477E-4 0.0 54-55 1.957465067078413E-4 0.0 3.9149301341568256E-5 1.1744790402470477E-4 0.0 56-57 2.3489580804940955E-4 0.0 3.9149301341568256E-5 1.1744790402470477E-4 0.0 58-59 3.327690614033302E-4 0.0 3.9149301341568256E-5 1.1744790402470477E-4 0.0 60-61 5.480902187819557E-4 0.0 3.9149301341568256E-5 1.1744790402470477E-4 0.0 62-63 6.459634721358762E-4 0.0 3.9149301341568256E-5 1.1744790402470477E-4 0.0 64-65 6.851127734774445E-4 0.0 3.9149301341568256E-5 1.1744790402470477E-4 0.0 66-67 7.046874241482286E-4 0.0 3.9149301341568256E-5 1.1744790402470477E-4 0.0 68-69 7.829860268313652E-4 0.0 3.9149301341568256E-5 1.1744790402470477E-4 0.0 70-71 8.808592801852858E-4 0.0 3.9149301341568256E-5 1.1744790402470477E-4 0.0 72-73 9.591578828684223E-4 0.0 3.9149301341568256E-5 1.1744790402470477E-4 0.0 74-75 0.001076605786893127 0.0 3.9149301341568256E-5 1.1744790402470477E-4 0.0 76-77 0.0011744790402470479 0.0 3.9149301341568256E-5 1.1744790402470477E-4 0.0 78-79 0.0014093748482964573 0.0 3.9149301341568256E-5 1.1744790402470477E-4 0.0 80-81 0.0017812932110413556 0.0 3.9149301341568256E-5 1.1744790402470477E-4 0.0 82-83 0.001996614368419981 0.0 3.9149301341568256E-5 1.1744790402470477E-4 0.0 84-85 0.002094487621773902 0.0 3.9149301341568256E-5 1.1744790402470477E-4 0.0 86-87 0.002153211573786254 0.0 3.9149301341568256E-5 1.1744790402470477E-4 0.0 88 0.002153211573786254 0.0 3.9149301341568256E-5 1.1744790402470477E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 10290 0.0 80.1019 8 GCTGCCG 20 0.00134617 79.452354 1 CACCGGC 10075 0.0 78.223885 9 GAGCTGC 35 1.7892835E-6 75.66891 1 CTTCACC 11360 0.0 75.57342 6 GTGTGGC 4265 0.0 74.287964 7 ATGCAGC 45 2.6611815E-9 73.01906 2 CCTTCAC 12735 0.0 71.72615 5 CGGGGCA 1210 0.0 71.37829 94 CGTAGTG 2120 0.0 68.6372 4 TTCACCG 12795 0.0 66.21717 7 TGTGGCA 4970 0.0 64.03346 8 GGCTGCA 50 1.9981417E-7 63.56188 1 CGGGCCC 720 0.0 61.281452 7 GCCTTCA 3805 0.0 61.18722 4 CAGCGTA 1005 0.0 61.137466 1 ATGTGTG 4690 0.0 58.94896 5 ACCTTCA 5270 0.0 58.16159 4 AGGCGCG 350 0.0 57.667896 5 GCGTAGT 1485 0.0 57.211826 3 >>END_MODULE