FastQCFastQC Report
Tue 7 Mar 2017
1NF_15A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_15A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2554324
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG102900.080.10198
GCTGCCG200.0013461779.4523541
CACCGGC100750.078.2238859
GAGCTGC351.7892835E-675.668911
CTTCACC113600.075.573426
GTGTGGC42650.074.2879647
ATGCAGC452.6611815E-973.019062
CCTTCAC127350.071.726155
CGGGGCA12100.071.3782994
CGTAGTG21200.068.63724
TTCACCG127950.066.217177
TGTGGCA49700.064.033468
GGCTGCA501.9981417E-763.561881
CGGGCCC7200.061.2814527
GCCTTCA38050.061.187224
CAGCGTA10050.061.1374661
ATGTGTG46900.058.948965
ACCTTCA52700.058.161594
AGGCGCG3500.057.6678965
GCGTAGT14850.057.2118263
Produced by FastQC (version 0.11.5)