FastQCFastQC Report
Tue 7 Mar 2017
1NF_16A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_16A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2682948
Sequences flagged as poor quality0
Sequence length95
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA157410.5867053703612594No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA69400.25867068612585853No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC68680.2559870709383857No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA66200.24674350751486798No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG65590.2444698890921479No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG61910.2307536336895087No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT60850.22680275577461806No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG58250.21711192315318822No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA55970.20861380839285742No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG51500.19195303077062992No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG49690.18520672036878835No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG48510.18080857325598557No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA47250.17611224667790804No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA43480.16206053937683473No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT42530.15851965822669692No Hit
CAGCAGGCGCGCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACGAAA39970.14897791533790442No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA38470.14338705036400257No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC36830.13727437132586992No Hit
CAGCAGAAGAGCGGCCGGGAGAGTTGAGGGTGGACGTCTCGAGCTCGAGG34580.12888807386501716No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC33950.12653991057597838No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT33430.1246017440516924No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA32690.12184358399790081No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA32290.120352686671527No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA32170.11990541747361484No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC30530.11379273843548215No Hit
CAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTTGGCACTCTCATGCGA30510.11371819356916348No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA30260.11278638274017984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTA401.6370905E-1188.999743
GCTGCCA450.088.999743
GCTGCAT650.088.999743
TGCTGCG202.4113406E-588.999742
AGCTGCA1300.088.999742
GCAGCCG256.816372E-788.999743
GCAGCTC158.578006E-488.999733
GCAGCCC158.578006E-488.999733
CAGCTGC6500.087.6305161
GCTGCCC2750.087.381563
CAGCAGC4100.086.829021
AGCAGCA1550.086.128782
AGCTGCC3000.086.033082
AGCAGCG1000.084.549752
AGCAGCC950.084.3155442
AGCTGCG850.083.764462
CAGCCCT156800.082.7845151
GCTGCTT700.082.642623
GCTGCAA600.081.583093
CTGCAGC1200.081.583091
Produced by FastQC (version 0.11.5)