##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1NF_16A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2682948
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.756528266667859	12.0	12.0	12.0	2.0	27.0
2	14.616355218215187	12.0	12.0	12.0	12.0	22.0
3	13.69188519494228	12.0	12.0	12.0	12.0	22.0
4	13.769083858501917	12.0	12.0	12.0	12.0	22.0
5	22.41668343926159	22.0	22.0	22.0	22.0	27.0
6	26.85825591848966	27.0	22.0	32.0	22.0	32.0
7	34.91908751120037	37.0	37.0	37.0	27.0	41.0
8	37.53472411690424	41.0	37.0	41.0	27.0	41.0
9	38.49810208770353	41.0	37.0	41.0	32.0	41.0
10-11	38.86060184543271	41.0	41.0	41.0	37.0	41.0
12-13	39.03872941257154	41.0	41.0	41.0	37.0	41.0
14-15	38.99543468602448	41.0	41.0	41.0	37.0	41.0
16-17	38.89854816418358	41.0	41.0	41.0	37.0	41.0
18-19	38.82589506020989	41.0	41.0	41.0	37.0	41.0
20-21	38.83546606195871	41.0	41.0	41.0	37.0	41.0
22-23	38.838272676175606	41.0	41.0	41.0	34.5	41.0
24-25	38.84124049366592	41.0	41.0	41.0	37.0	41.0
26-27	38.80744874667716	41.0	41.0	41.0	37.0	41.0
28-29	38.75253955723331	41.0	41.0	41.0	32.0	41.0
30-31	38.638071442308984	41.0	41.0	41.0	32.0	41.0
32-33	38.683837144812344	41.0	41.0	41.0	32.0	41.0
34-35	38.65519495718888	41.0	41.0	41.0	32.0	41.0
36-37	38.577117036931014	41.0	41.0	41.0	32.0	41.0
38-39	38.47992450841388	41.0	41.0	41.0	32.0	41.0
40-41	38.51987030684158	41.0	41.0	41.0	32.0	41.0
42-43	38.26841258198072	41.0	41.0	41.0	32.0	41.0
44-45	38.37334454488123	41.0	41.0	41.0	32.0	41.0
46-47	38.368801408003435	41.0	41.0	41.0	32.0	41.0
48-49	38.33262981615745	41.0	41.0	41.0	32.0	41.0
50-51	38.222577925476	41.0	41.0	41.0	32.0	41.0
52-53	38.19610107985693	41.0	41.0	41.0	32.0	41.0
54-55	38.05864090545177	41.0	37.0	41.0	32.0	41.0
56-57	37.84513378567158	41.0	37.0	41.0	27.0	41.0
58-59	37.835180555120715	41.0	37.0	41.0	27.0	41.0
60-61	37.833665057988455	41.0	37.0	41.0	27.0	41.0
62-63	37.8187724845953	41.0	37.0	41.0	27.0	41.0
64-65	37.81572434501153	41.0	37.0	41.0	27.0	41.0
66-67	37.6804902666768	41.0	37.0	41.0	27.0	41.0
68-69	37.70775747424102	41.0	37.0	41.0	27.0	41.0
70-71	37.68247670100203	41.0	37.0	41.0	27.0	41.0
72-73	37.52186400929127	41.0	37.0	41.0	27.0	41.0
74-75	37.44832531230572	41.0	37.0	41.0	27.0	41.0
76-77	36.38499180751919	39.0	34.5	41.0	24.5	41.0
78-79	36.9025980004085	41.0	37.0	41.0	27.0	41.0
80-81	37.57795380305544	41.0	37.0	41.0	27.0	41.0
82-83	37.748653346989954	41.0	37.0	41.0	27.0	41.0
84-85	37.67480901605249	41.0	37.0	41.0	27.0	41.0
86-87	37.54113050271567	41.0	37.0	41.0	27.0	41.0
88-89	37.44692480062976	41.0	37.0	41.0	27.0	41.0
90-91	37.281299712107725	41.0	37.0	41.0	27.0	41.0
92-93	37.176222759442226	41.0	37.0	41.0	27.0	41.0
94-95	37.01401797574907	41.0	37.0	41.0	27.0	41.0
96-97	36.938265296233844	41.0	37.0	41.0	27.0	41.0
98-99	36.825655771189005	41.0	37.0	41.0	27.0	41.0
100	36.683512688281695	41.0	37.0	41.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.6027226078693655
1101	2	-0.48543300133261624
1101	3	0.011470998674726118
1101	4	0.16206199043264746
1101	5	-1.1072998943239973
1101	6	-0.8219991090579519
1101	7	-0.3342346139114767
1101	8	-0.2634154857187667
1101	9	-0.19964793206018072
1101	10-11	-0.03265796759058048
1101	12-13	0.01433590652575134
1101	14-15	-0.07073503432044959
1101	16-17	-0.12931218504539288
1101	18-19	0.0033136091991465833
1101	20-21	-0.01666362748315464
1101	22-23	0.11264607695770934
1101	24-25	0.13375939862157793
1101	26-27	0.05181057619068952
1101	28-29	-0.13599360719921094
1101	30-31	0.009158864588997062
1101	32-33	-0.09469824041650554
1101	34-35	-0.104032958059328
1101	36-37	0.14703580300120223
1101	38-39	0.04217594156571636
1101	40-41	0.013694504196777757
1101	42-43	0.06278970371637627
1101	44-45	-0.05613694881129305
1101	46-47	0.01909999933425155
1101	48-49	0.004093530159998693
1101	50-51	-0.01945758128243824
1101	52-53	0.15898527521396488
1101	54-55	0.06219011576423128
1101	56-57	0.09836251270417762
1101	58-59	0.21878979805568122
1101	60-61	-0.0370522454907487
1101	62-63	0.006018871417730054
1101	64-65	-0.08273663212793281
1101	66-67	-0.06006479568199552
1101	68-69	-0.039835655425441985
1101	70-71	-0.0854385042218695
1101	72-73	0.061927008811920814
1101	74-75	0.1881165337921189
1101	76-77	-0.35629305753158036
1101	78-79	-0.07607943032903819
1101	80-81	-0.1661152359305902
1101	82-83	-0.2038278988715163
1101	84-85	-0.053021151668687594
1101	86-87	-0.1153537023558826
1101	88-89	-0.3732285264843682
1101	90-91	-0.07319575148066804
1101	92-93	-0.013528426659192405
1101	94-95	0.2164384421526293
1101	96-97	-0.03512970832755968
1101	98-99	-0.26387727310016373
1101	100	-0.48819090235306817
1102	1	1.6883228044268392
1102	2	0.8270875493491019
1102	3	0.007412808622021316
1102	4	0.1960749635175425
1102	5	-0.7921964282487508
1102	6	-0.18295274088742985
1102	7	-0.16975989844956274
1102	8	-0.4505586511543953
1102	9	-0.3506703808775953
1102	10-11	-0.5140605575415051
1102	12-13	-0.49323011629853397
1102	14-15	-0.3372083878782064
1102	16-17	-0.3833344683637421
1102	18-19	-0.19083310097845896
1102	20-21	-0.29469803816433426
1102	22-23	-0.5069320972887468
1102	24-25	-0.3691550616947339
1102	26-27	-0.3634646589528572
1102	28-29	-0.4660437526852803
1102	30-31	-0.5130100807882982
1102	32-33	-0.46916957299014683
1102	34-35	-0.3684513345791629
1102	36-37	-0.4319697663702371
1102	38-39	-0.5035562969949794
1102	40-41	-0.3409138426425855
1102	42-43	-0.39771006334552084
1102	44-45	-0.4839252252078481
1102	46-47	-0.5797171562837136
1102	48-49	-0.519699784844498
1102	50-51	-0.5200398354087952
1102	52-53	-0.5166709249130363
1102	54-55	-0.5862465013766567
1102	56-57	-0.3960309332414269
1102	58-59	-0.5154197142761916
1102	60-61	-0.3861321399796296
1102	62-63	-0.5054032977281437
1102	64-65	-0.48037710401168
1102	66-67	-0.3049871824554842
1102	68-69	-0.4675729880034467
1102	70-71	-0.6233425278904221
1102	72-73	-0.5347202499914232
1102	74-75	-0.6241604161258252
1102	76-77	-0.4399635860860016
1102	78-79	-0.5035891105659047
1102	80-81	-0.5207071786781228
1102	82-83	-0.5082687963867656
1102	84-85	-0.6105633338795045
1102	86-87	-0.46821847650069515
1102	88-89	-0.4791108333225367
1102	90-91	-0.6101428697920852
1102	92-93	-0.42860854424372974
1102	94-95	-0.33186677821264965
1102	96-97	-0.46098549157191115
1102	98-99	-0.48304742290233094
1102	100	-0.7393479376306118
1103	1	1.6131987997265007
1103	2	0.5971672402360433
1103	3	0.38913409014456946
1103	4	1.1158993216354585
1103	5	2.8148431502821047
1103	6	0.09229512910902571
1103	7	-0.6076378465235379
1103	8	-0.38836745473533796
1103	9	-0.35451106006982513
1103	10-11	-0.17998131869597955
1103	12-13	-0.44269376320957576
1103	14-15	-0.32305393395055404
1103	16-17	-0.34597469506846323
1103	18-19	-0.22619818576038853
1103	20-21	-0.12872263055569277
1103	22-23	-0.31917022646180726
1103	24-25	-0.4831182902072513
1103	26-27	-0.3472228591737405
1103	28-29	-0.31365858365859367
1103	30-31	-0.30188090289029645
1103	32-33	-0.4794061177877822
1103	34-35	-0.5183186775235811
1103	36-37	-0.35234142545517244
1103	38-39	-0.14653326109820597
1103	40-41	-0.2767628087651275
1103	42-43	-0.3157152893309103
1103	44-45	-0.46081302785905365
1103	46-47	-0.6217029192095467
1103	48-49	-0.4747168311925023
1103	50-51	-0.41924881869106656
1103	52-53	-0.20509701037934747
1103	54-55	-0.3654980605663809
1103	56-57	-0.2805084220621339
1103	58-59	-0.40044394644704084
1103	60-61	-0.3431892276804902
1103	62-63	-0.3669447961339216
1103	64-65	-0.5587312903575778
1103	66-67	-0.3706092096604934
1103	68-69	-0.5072737298941234
1103	70-71	-0.5504538233868459
1103	72-73	-0.35398723286402856
1103	74-75	-0.3562831260397772
1103	76-77	-0.4698973895275387
1103	78-79	-0.44789089802539905
1103	80-81	-0.2954882304104771
1103	82-83	-0.32595800229943706
1103	84-85	-0.3204357362677399
1103	86-87	-0.3211343852730906
1103	88-89	-0.2662628013941344
1103	90-91	-0.38188091327801743
1103	92-93	-0.19836741955278114
1103	94-95	-0.4147541109786985
1103	96-97	-0.2223916377738533
1103	98-99	-0.23039260894005054
1103	100	-0.29521836085066866
1104	1	1.9116056593645538
1104	2	-0.6430852514679355
1104	3	0.02477739128380918
1104	4	0.019326822629677665
1104	5	-1.0730136909203445
1104	6	-0.4134362065631052
1104	7	-0.3561946901164603
1104	8	-0.19457847828437735
1104	9	0.11138850682026913
1104	10-11	0.1667335220008468
1104	12-13	-0.08405699095411023
1104	14-15	-0.040976165404664755
1104	16-17	0.03179834201063869
1104	18-19	-0.11385527774499593
1104	20-21	0.03551668168402955
1104	22-23	0.04023855931648512
1104	24-25	-0.015752386583805844
1104	26-27	-0.09381317805535616
1104	28-29	0.0884349619968674
1104	30-31	0.0031513648482714984
1104	32-33	0.056236350308481065
1104	34-35	-0.08279511725726962
1104	36-37	-0.004789195471566643
1104	38-39	-0.11347884021157029
1104	40-41	-0.018133932308302292
1104	42-43	-0.117773231453981
1104	44-45	0.019405642300107218
1104	46-47	-0.18235768374452022
1104	48-49	-0.39002732804106444
1104	50-51	-0.19717241923319762
1104	52-53	-0.1256406469813669
1104	54-55	-0.15420667010175748
1104	56-57	-0.06670921004835151
1104	58-59	-0.11886564149391177
1104	60-61	-0.03286605046907454
1104	62-63	0.02807875000688398
1104	64-65	-0.05006999766321485
1104	66-67	-0.37186058508688546
1104	68-69	-0.23288241266484278
1104	70-71	-0.21839836590500283
1104	72-73	-0.18707638802082727
1104	74-75	-0.49500361549235805
1104	76-77	-0.3504096892259767
1104	78-79	-0.21850400316773033
1104	80-81	-0.31409659952239366
1104	82-83	-0.243449622973678
1104	84-85	-0.043845547775468674
1104	86-87	-0.1306618253465146
1104	88-89	-0.19488894691878045
1104	90-91	-0.07230747474464039
1104	92-93	-0.13138243388473825
1104	94-95	-0.2517375609501258
1104	96-97	-0.16351197916210936
1104	98-99	-0.42293528066099384
1104	100	-0.11428251312803184
1105	1	1.5493478666097094
1105	2	0.3636544283972718
1105	3	-0.021557907789484076
1105	4	-0.3176571706306426
1105	5	-1.157259689235424
1105	6	0.364996817868235
1105	7	0.16823664939491323
1105	8	0.1537787247484772
1105	9	0.10254267699718156
1105	10-11	-0.008498154494517962
1105	12-13	-0.0566770415017146
1105	14-15	0.07928499719011484
1105	16-17	-0.0143263420668589
1105	18-19	0.08601835325753626
1105	20-21	0.005398737286384403
1105	22-23	0.11795549276214246
1105	24-25	0.04553657719041837
1105	26-27	0.0011741850449027424
1105	28-29	-0.010764701019134293
1105	30-31	-0.10931704290236866
1105	32-33	-0.028009648006595
1105	34-35	0.0010296510662328728
1105	36-37	-0.07134353414048178
1105	38-39	0.07438973603105126
1105	40-41	0.05852992615842112
1105	42-43	0.22805659162941794
1105	44-45	0.025513477177959487
1105	46-47	-0.10674690025673073
1105	48-49	-0.01955555045049806
1105	50-51	-0.12830131561061364
1105	52-53	-0.07109136307235531
1105	54-55	0.10042685980556598
1105	56-57	0.05313072255484741
1105	58-59	0.03635761040162322
1105	60-61	0.04758887792183231
1105	62-63	-0.06037954822394909
1105	64-65	0.05060397032330144
1105	66-67	-0.060571621312668356
1105	68-69	-0.10988325512482078
1105	70-71	-0.04485160937593946
1105	72-73	-0.1784411731935336
1105	74-75	-0.15801962223203958
1105	76-77	-0.07197666479464004
1105	78-79	-0.10919482035391326
1105	80-81	-0.23364167113149392
1105	82-83	-0.28346647217333754
1105	84-85	-0.12893400607369898
1105	86-87	-0.26566603764642593
1105	88-89	-0.16645592248743668
1105	90-91	-0.29471691029644376
1105	92-93	-0.11032093430597456
1105	94-95	-0.28269796533091807
1105	96-97	-0.05020111142832917
1105	98-99	-0.2043169150333668
1105	100	-0.22169616097975364
1106	1	1.3958232018765422
1106	2	-0.6427803678633843
1106	3	-0.18385761327893135
1106	4	-0.2795572332894807
1106	5	-1.1812070698296893
1106	6	-0.5895310190561851
1106	7	0.07378055473661504
1106	8	-0.04348832665559854
1106	9	0.02514744832046034
1106	10-11	-0.019893266824851707
1106	12-13	-0.0467522040712538
1106	14-15	0.04609561330730827
1106	16-17	-0.044194772526061854
1106	18-19	-0.010223413132941062
1106	20-21	0.04241160687890755
1106	22-23	0.050737944643096
1106	24-25	-0.18094761021417582
1106	26-27	-0.1299168286685557
1106	28-29	-0.15317548226409627
1106	30-31	0.03091890196346725
1106	32-33	-0.08758530609961213
1106	34-35	-0.08751958658457681
1106	36-37	-0.13392124441555353
1106	38-39	-0.017886966516087455
1106	40-41	-0.1524749351580823
1106	42-43	0.028678834535440956
1106	44-45	-0.043487289586785494
1106	46-47	-0.22764236092535128
1106	48-49	-0.2686962693029571
1106	50-51	-0.13460750005188515
1106	52-53	-0.22838204120358085
1106	54-55	-0.2587813960219165
1106	56-57	-0.05766257757537119
1106	58-59	-0.2073766810839217
1106	60-61	-0.1067242788920737
1106	62-63	-0.12650091713030065
1106	64-65	-0.29537829456237574
1106	66-67	-0.3721493484089038
1106	68-69	-0.22668591623303058
1106	70-71	-0.5202751873217082
1106	72-73	-0.31126143765026626
1106	74-75	-0.49018452711536753
1106	76-77	-0.07219411338846271
1106	78-79	-0.250935908991643
1106	80-81	-0.24158283770727706
1106	82-83	-0.31280941646584637
1106	84-85	-0.44090545354890764
1106	86-87	-0.12844178208808898
1106	88-89	-0.20390631757453548
1106	90-91	-0.43166484318561515
1106	92-93	-0.43992884698170087
1106	94-95	-0.23684662517473498
1106	96-97	-0.26078369143048263
1106	98-99	-0.5777954757584709
1106	100	-0.6036084330004101
1107	1	1.3885537812843651
1107	2	-0.5552289648106843
1107	3	-0.24140968470323187
1107	4	-0.12187606061391776
1107	5	-1.1875891391785203
1107	6	-0.20075139524386287
1107	7	-0.6150099740860284
1107	8	-0.4828517202948106
1107	9	-0.3793104143678647
1107	10-11	-0.2674333955039714
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2224	42-43	0.27953180397190636
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2224	46-47	0.25730574406950524
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2224	52-53	0.29828074947806726
2224	54-55	0.22385318015394517
2224	56-57	0.4529666278224269
2224	58-59	0.43690318525318617
2224	60-61	0.38660084755418467
2224	62-63	0.45517926378882123
2224	64-65	0.3721359422729478
2224	66-67	0.4406529818691354
2224	68-69	0.4493304442377095
2224	70-71	0.4917856281929005
2224	72-73	0.33561576370014734
2224	74-75	0.45022427098005124
2224	76-77	0.6521201336480331
2224	78-79	0.3645027344181955
2224	80-81	0.27031272574881626
2224	82-83	0.2668425926821172
2224	84-85	0.1625714566546037
2224	86-87	0.3675911346055756
2224	88-89	0.4707819953311798
2224	90-91	0.3772119321970351
2224	92-93	0.3951320222958188
2224	94-95	0.5847290557878182
2224	96-97	0.41210735456162695
2224	98-99	0.48563477787845244
2224	100	0.5726329735479112
2225	1	1.661083837136184
2225	2	1.5853261875459257
2225	3	0.9098074502001232
2225	4	1.1292818215748568
2225	5	3.0522809255787386
2225	6	-0.05766801019588286
2225	7	0.20446173011320212
2225	8	0.3996778447061189
2225	9	0.22697317823991625
2225	10-11	0.3233154676503034
2225	12-13	0.18320208714598607
2225	14-15	0.06688979823403685
2225	16-17	0.21294644871106527
2225	18-19	0.11289953286915733
2225	20-21	0.17903333738334481
2225	22-23	0.3031896351644221
2225	24-25	0.2708574245123643
2225	26-27	0.169051102060493
2225	28-29	0.13447424745668002
2225	30-31	0.1481571698585995
2225	32-33	0.2402413813174391
2225	34-35	0.22385787165942617
2225	36-37	0.21123525263330833
2225	38-39	0.12468463195301638
2225	40-41	0.10087768609504622
2225	42-43	0.34839162664808754
2225	44-45	0.30404639528888566
2225	46-47	0.22548026775046282
2225	48-49	0.26285872610271355
2225	50-51	0.13812927365032124
2225	52-53	0.25017980844998533
2225	54-55	0.13929650344360311
2225	56-57	0.21751450429204766
2225	58-59	0.2835160543514945
2225	60-61	0.28270751389109705
2225	62-63	0.1600002293833782
2225	64-65	0.2271770512818918
2225	66-67	0.26163334654671644
2225	68-69	0.3758525561306101
2225	70-71	0.221180802281026
2225	72-73	0.3392150346747371
2225	74-75	0.1978409583248748
2225	76-77	0.3828621361458957
2225	78-79	0.18187300815308305
2225	80-81	0.23141579916934774
2225	82-83	0.3130711639929231
2225	84-85	0.12013708748965257
2225	86-87	0.28479983015022015
2225	88-89	0.2043267130571067
2225	90-91	0.1561472748156234
2225	92-93	0.33402204470517916
2225	94-95	0.6009754955030715
2225	96-97	0.46255262201062664
2225	98-99	0.2914402231733817
2225	100	0.2515148689590063
2226	1	1.383135405454226
2226	2	1.59781077671874
2226	3	0.7883988919328164
2226	4	0.9266004242710846
2226	5	3.1967754054013824
2226	6	-0.3843695768853479
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2226	10-11	0.06397039055632092
2226	12-13	0.28412706595717907
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2226	18-19	0.14730907729080656
2226	20-21	0.005462810715947342
2226	22-23	0.21694938149476428
2226	24-25	0.29636689138609995
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2226	32-33	0.12703036092143094
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2226	74-75	0.4823555805057964
2226	76-77	0.5190327768828737
2226	78-79	0.4795129945977905
2226	80-81	0.40263468038660477
2226	82-83	0.1285370335585867
2226	84-85	0.2774093866707261
2226	86-87	0.28797451252606265
2226	88-89	0.3259861412261955
2226	90-91	0.2552171503993961
2226	92-93	0.3347804583757892
2226	94-95	0.40209526231880943
2226	96-97	0.311220118211196
2226	98-99	0.31694639824591775
2226	100	0.3281126572844073
2227	1	1.2019234748493268
2227	2	1.5379475041200745
2227	3	0.7509736862142784
2227	4	0.07839989910335987
2227	5	3.184759239271969
2227	6	0.38623112525279524
2227	7	0.37695584706143137
2227	8	0.41612483369564046
2227	9	0.25154212857363234
2227	10-11	0.22813055644346036
2227	12-13	0.22915110438106012
2227	14-15	0.297711256678312
2227	16-17	0.2098830139758192
2227	18-19	0.2496465557721379
2227	20-21	0.2400847200645586
2227	22-23	0.15471855519015065
2227	24-25	0.2855110928636506
2227	26-27	0.23327392795600588
2227	28-29	0.26488295982984766
2227	30-31	0.21769857155482697
2227	32-33	0.0995422495985494
2227	34-35	0.21034446548468821
2227	36-37	0.2370933113609155
2227	38-39	0.2629778769594111
2227	40-41	0.21116211360348558
2227	42-43	0.43783430289617087
2227	44-45	0.39884123270807237
2227	46-47	0.28939245055433815
2227	48-49	0.17451322951236392
2227	50-51	0.31010182447387535
2227	52-53	0.21680363434342098
2227	54-55	0.3792344473489493
2227	56-57	0.4745541360651586
2227	58-59	0.56870100354692
2227	60-61	0.4166551227854072
2227	62-63	0.47181112492648936
2227	64-65	0.3722752621572596
2227	66-67	0.4828562960778342
2227	68-69	0.2307813689594269
2227	70-71	0.44595369956056174
2227	72-73	0.5159278712968529
2227	74-75	0.336265263979719
2227	76-77	0.603733259496245
2227	78-79	0.47059650851880974
2227	80-81	0.18219276951682417
2227	82-83	0.30912561684320394
2227	84-85	0.21014078619728593
2227	86-87	0.29605757723321346
2227	88-89	0.2934659122437395
2227	90-91	0.4485981469301237
2227	92-93	0.3300207233928134
2227	94-95	0.43238073502806884
2227	96-97	0.3110581679999669
2227	98-99	0.6267917005497949
2227	100	0.6376102148037148
2228	1	1.5933288748660956
2228	2	1.668241499370433
2228	3	-0.13121178872993333
2228	4	-0.08206875638829736
2228	5	3.075334666108553
2228	6	0.02724263020827067
2228	7	0.4410317393751413
2228	8	0.5052276758178849
2228	9	0.37948058745264035
2228	10-11	0.17976527044835677
2228	12-13	0.23364497742107915
2228	14-15	0.2328894129481185
2228	16-17	0.1665518885094528
2228	18-19	0.15358850224304632
2228	20-21	0.13023065450450844
2228	22-23	0.17195033421841543
2228	24-25	0.2236258786451728
2228	26-27	0.1724374362021024
2228	28-29	0.20428975240403702
2228	30-31	0.17920747846439156
2228	32-33	0.07737281454677003
2228	34-35	0.2788061905582495
2228	36-37	0.2579639386780812
2228	38-39	0.16347622243658577
2228	40-41	0.16006629246378168
2228	42-43	0.34888908766874493
2228	44-45	0.024038998073500295
2228	46-47	0.14957842790737885
2228	48-49	0.1904893308302107
2228	50-51	0.14226912626817523
2228	52-53	0.19433228912227207
2228	54-55	0.3053441932020533
2228	56-57	0.32296510028549363
2228	58-59	0.27389136191180086
2228	60-61	0.2617462803728685
2228	62-63	0.28844903537410005
2228	64-65	0.19214508351976178
2228	66-67	0.3470144539626432
2228	68-69	0.37877305080482415
2228	70-71	0.3784111347032493
2228	72-73	0.18567628293256178
2228	74-75	0.2437841615590841
2228	76-77	0.4323498669832375
2228	78-79	0.4398467812882316
2228	80-81	0.2518333819184093
2228	82-83	0.26704586469363534
2228	84-85	0.23073154184046984
2228	86-87	0.34505354298332946
2228	88-89	0.3091347659441084
2228	90-91	0.08133342207299421
2228	92-93	0.20155747284215408
2228	94-95	0.409050663277263
2228	96-97	0.2892528846494784
2228	98-99	0.6613243871680723
2228	100	0.6399509440609705
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	13.0
13	43.0
14	159.0
15	523.0
16	1808.0
17	4336.0
18	7554.0
19	9308.0
20	10922.0
21	12734.0
22	14679.0
23	16861.0
24	19242.0
25	21817.0
26	24498.0
27	27596.0
28	31772.0
29	35483.0
30	40794.0
31	47237.0
32	55356.0
33	65464.0
34	80684.0
35	104267.0
36	145835.0
37	243036.0
38	590415.0
39	1069270.0
40	1242.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.133647231397205	20.00725868245241	19.13352527207443	36.725568814075956
2	19.794743378902844	51.45691720702493	12.05149270087685	16.696846713195377
3	38.91499201624482	17.061679913289414	23.453939472550346	20.569388597915427
4	19.832736228954122	16.00914367330265	21.36783866105493	42.790281436688296
5	17.93497302221288	36.2083051926463	26.43413141067214	19.422590374468683
6	17.8836116093193	27.518572853443303	31.150324195623618	23.447491341613777
7	20.41884524038483	27.021656774562906	35.851235282979765	16.708262702072496
8	20.493129199671404	27.943254956860887	34.279270414484365	17.284345428983343
9	21.117479727523605	28.150526957660006	27.663599890866315	23.068393423950074
10-11	19.635378695375387	29.936566046006106	28.912822760634942	21.51523249798356
12-13	23.974262639454807	26.692243010300608	27.29452825772248	22.038966092522106
14-15	21.93219920773716	27.392964753696308	28.37714335126883	22.297692687297705
16-17	21.124188765492285	29.352507018399166	28.23349539387271	21.28980882223584
18-19	21.50261577935912	29.64925149499729	29.677094002567323	19.171038723076258
20-21	22.696321359936906	29.06946016098709	29.877824691346984	18.35639378772902
22-23	21.893640875633817	30.02150992117626	28.676944167386026	19.4079050358039
24-25	22.10870281496324	28.474331220731823	26.51680166741957	22.900164296885368
26-27	20.2623755659819	26.402598932219334	29.946480513226497	23.38854498857227
28-29	19.754332750141824	27.894867492278983	30.712077580622438	21.638722176956755
30-31	20.098926897201615	30.13192365269461	28.098981320228074	21.670168129875705
32-33	20.810019426392163	30.06027325166198	28.601467490238345	20.52823983170751
34-35	21.40477564231584	29.040834932320713	29.91889891268858	19.635490512674863
36-37	22.13632168793432	27.930060515522477	28.753818560777173	21.17979923576603
38-39	21.49405826284907	29.314880853097968	28.797125527991348	20.393935356061615
40-41	20.748018758707882	29.63568716701318	29.836122216366235	19.780171857912702
42-43	20.75364557349376	29.797213547572994	29.207488560613697	20.24165231831955
44-45	20.81868526710171	29.904008575641424	28.638497652582164	20.638808504674707
46-47	20.079239311554666	27.89934568582968	28.85072806871557	23.170686933900086
48-49	21.082089552238806	28.26245607443752	30.116852805197862	20.53860156812581
50-51	22.12702221586106	30.53922401775957	28.179841726339834	19.15391204003954
52-53	20.497209377564843	30.14955007875765	28.32474317104462	21.02849737263289
54-55	22.22797152900204	28.339793226264508	28.119994080036566	21.31224116469689
56-57	21.58957539615161	28.771494343194153	29.139152708316594	20.49977755233764
58-59	21.137492346418124	27.924795927781876	29.025439248033834	21.91227247776617
60-61	19.773569293387105	30.137554324733312	29.740042639790676	20.348833742088903
62-63	20.40258244116697	31.11976174354105	27.13980606156847	21.337849753723503
64-65	20.066620809107004	30.0844165489012	28.780029262614544	21.068933379377256
66-67	20.22115225490766	30.109342409916255	29.605847746583237	20.063657588592847
68-69	20.885440194890098	29.80736115645924	29.230700706834423	20.07649794181624
70-71	19.701388174500586	30.62083201016196	29.478077845712995	20.19970196962446
72-73	20.270314594244837	30.611215722406843	28.381112865400297	20.73735681794802
74-75	20.045915910408997	28.92502948249463	30.608233927754096	20.420820679342274
76-77	19.76769583309106	30.0591550786672	28.73410144363588	21.439047644605857
78-79	19.657555793105196	29.247119213641113	28.42086391536474	22.67446107788895
80-81	20.874065136967882	29.347808463567947	28.402075852052956	21.376050547411214
82-83	20.196216997124058	28.24928026931569	30.111336485090277	21.443166248469968
84-85	20.243049809388776	29.079821897405388	28.572245902641423	22.10488239056441
86-87	22.13798031120991	28.302113943319064	28.924209488965126	20.635696256505902
88-89	21.84447853629664	27.579326919493035	29.034666344632843	21.54152819957748
90-91	21.792390310956456	28.287670875469818	28.554373770941517	21.36556504263221
92-93	21.43664357266708	28.702419875450435	29.835352753761907	20.025583798120575
94-95	22.055869140959867	28.601150674556497	28.588627137014953	20.75435304746868
96-97	21.406322448291952	28.803111353630413	29.112752092101672	20.677814105975965
98-99	22.474643563721695	28.809298577534857	28.549248811382107	20.166809047361337
100	22.986207708833717	30.15690203462758	27.148681226769956	19.708209029768746
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	11.5
2	16.5
3	16.5
4	26.0
5	38.0
6	45.0
7	49.5
8	55.0
9	54.5
10	64.5
11	83.5
12	104.0
13	122.0
14	139.5
15	180.5
16	250.0
17	337.5
18	471.0
19	770.5
20	1293.0
21	1975.0
22	2975.5
23	4650.0
24	7077.0
25	10348.0
26	14831.5
27	19897.0
28	25210.0
29	31459.5
30	38709.5
31	47207.0
32	57120.0
33	67898.5
34	79466.5
35	91763.0
36	103735.0
37	112804.0
38	118483.5
39	123132.5
40	126243.0
41	126489.5
42	124871.5
43	123775.5
44	123835.5
45	121132.0
46	113411.5
47	105138.5
48	99344.5
49	94694.0
50	90171.0
51	83688.5
52	74677.5
53	64599.0
54	53414.5
55	42406.0
56	32817.0
57	24102.0
58	16861.0
59	11986.0
60	9144.5
61	7536.0
62	6552.5
63	5517.5
64	4750.5
65	5125.5
66	5629.5
67	4583.0
68	2876.0
69	2694.0
70	3675.5
71	3559.5
72	2110.5
73	888.0
74	396.0
75	229.0
76	157.0
77	120.5
78	90.0
79	65.0
80	53.0
81	50.5
82	40.5
83	26.5
84	18.5
85	21.5
86	20.0
87	18.5
88	22.5
89	18.0
90	10.5
91	14.5
92	14.0
93	15.5
94	18.5
95	17.5
96	17.0
97	19.0
98	49.0
99	111.0
100	107.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.372042991515304
2	0.005963589305495298
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	3.7272433159345615E-4
30-31	0.00969083262142986
32-33	0.0
34-35	0.0
36-37	0.0
38-39	7.64084879766585E-4
40-41	0.001267262727417751
42-43	9.318108289836404E-5
44-45	0.0
46-47	0.002329527072459101
48-49	0.0
50-51	0.0
52-53	0.001267262727417751
54-55	0.01859894414651346
56-57	0.010976731565427285
58-59	0.014182160817131006
60-61	2.9817946527476493E-4
62-63	0.010902186699108592
64-65	9.318108289836404E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	5.590864973901843E-5
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2682948.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.60449027594353
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.87054902710611	64.80933030158613
2	8.181599062136224	12.20768055345621
3	2.213290575041932	4.953642455506597
4	0.9692766275055263	2.8924955492574154
5	0.4813443569069988	1.7955225197124243
6	0.3095787046502559	1.3857576876431525
7	0.20540049299753393	1.072665935775597
8	0.14853373793061195	0.8865027045675107
9	0.10841013084667703	0.7279094297308214
>10	0.47460389894541066	6.101435972463349
>50	0.02540238987681134	1.298996511567414
>100	0.011247874227818288	1.4562879217373643
>500	6.104974625260875E-4	0.2928218735191253
>1k	1.5262436561422167E-4	0.11895058347686693
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	1.8636216579672808E-5	0.0	0.0	0.0	0.0
36-37	3.7272433159345616E-5	0.0	0.0	0.0	0.0
38-39	3.7272433159345616E-5	0.0	0.0	0.0	0.0
40-41	3.7272433159345616E-5	0.0	0.0	0.0	0.0
42-43	3.7272433159345616E-5	0.0	0.0	0.0	0.0
44-45	3.7272433159345616E-5	0.0	0.0	0.0	0.0
46-47	9.318108289836404E-5	0.0	0.0	0.0	0.0
48-49	1.1181729947803684E-4	0.0	0.0	0.0	0.0
50-51	1.1181729947803684E-4	0.0	0.0	0.0	0.0
52-53	1.3045351605770966E-4	0.0	0.0	0.0	0.0
54-55	1.6772594921705527E-4	0.0	0.0	0.0	0.0
56-57	2.0499838237640088E-4	0.0	3.1681568185443773E-4	0.0	0.0
58-59	2.2363459895607368E-4	0.0	6.336313637088755E-4	0.0	0.0
60-61	2.609070321154193E-4	0.0	6.336313637088755E-4	0.0	0.0
62-63	2.609070321154193E-4	0.0	6.336313637088755E-4	0.0	0.0
64-65	2.609070321154193E-4	0.0	6.336313637088755E-4	0.0	0.0
66-67	2.9817946527476493E-4	0.0	6.336313637088755E-4	0.0	0.0
68-69	3.5408811501378334E-4	0.0	6.336313637088755E-4	0.0	0.0
70-71	3.7272433159345615E-4	0.0	6.336313637088755E-4	0.0	0.0
72-73	3.9136054817312895E-4	0.0	6.336313637088755E-4	0.0	0.0
74-75	6.149951471292026E-4	0.0	6.336313637088755E-4	0.0	0.0
76-77	7.454486631869123E-4	0.0	6.336313637088755E-4	0.0	0.0
78-79	9.69083262142986E-4	0.0	6.336313637088755E-4	0.0	0.0
80-81	0.0013604438103161148	0.0	6.336313637088755E-4	0.0	0.0
82-83	0.001453624893214479	0.0	6.336313637088755E-4	0.0	0.0
84-85	0.0015840784092721887	0.0	6.336313637088755E-4	0.0	0.0
86-87	0.0016772594921705527	3.7272433159345616E-5	6.336313637088755E-4	0.0	0.0
88	0.0016772594921705527	3.7272433159345616E-5	6.336313637088755E-4	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	9940	0.0	79.09647	8
AGGCGCG	560	0.0	77.11316	5
CACCGGC	10055	0.0	74.41062	9
CTTCACC	11300	0.0	73.23228	6
CCTTCAC	12585	0.0	70.3051	5
GGCGCGC	620	0.0	69.6506	6
GTGTGGC	4470	0.0	68.46502	7
CGGGGCA	1290	0.0	67.67869	94
CGTAGTG	2165	0.0	67.42659	4
TTCACCG	12650	0.0	64.82329	7
AGGCTGC	80	0.0	64.54517	2
GCCTTCA	4020	0.0	64.21926	4
CGGGCCC	830	0.0	63.338623	7
AACGGGC	850	0.0	62.952732	5
GGCAGCC	60	9.955329E-9	61.79587	1
GCGCGCA	695	0.0	60.783592	7
TGTGGCA	4985	0.0	60.732788	8
GGGCCCG	865	0.0	60.233147	8
ACGGGCC	880	0.0	59.739838	6
ACCTTCA	4960	0.0	57.82135	4
>>END_MODULE