FastQCFastQC Report
Tue 7 Mar 2017
1NF_16A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_16A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2682948
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG99400.079.096478
AGGCGCG5600.077.113165
CACCGGC100550.074.410629
CTTCACC113000.073.232286
CCTTCAC125850.070.30515
GGCGCGC6200.069.65066
GTGTGGC44700.068.465027
CGGGGCA12900.067.6786994
CGTAGTG21650.067.426594
TTCACCG126500.064.823297
AGGCTGC800.064.545172
GCCTTCA40200.064.219264
CGGGCCC8300.063.3386237
AACGGGC8500.062.9527325
GGCAGCC609.955329E-961.795871
GCGCGCA6950.060.7835927
TGTGGCA49850.060.7327888
GGGCCCG8650.060.2331478
ACGGGCC8800.059.7398386
ACCTTCA49600.057.821354
Produced by FastQC (version 0.11.5)