FastQCFastQC Report
Tue 7 Mar 2017
1NF_17A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_17A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3119328
Sequences flagged as poor quality0
Sequence length95
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA151330.48513654222960845No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG78310.2510476615476154No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG73360.23517885903630528No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC62670.20090865724925366No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA61990.19872870054062924No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT59450.19058592107017922No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG57320.18375752726228214No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG55090.1766085515854697No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT52710.16897870310528423No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA50620.16227854204495326No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA50090.1605794581397019No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA43440.1392607638568307No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA41210.13211178818001826No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC40030.12832892212681707No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG38710.12409724145713436No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT37810.12121200463689616No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA36910.11832676781665795No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA36740.11778177863950184No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTTAAAAAAAAGCCATGAGTGCTC36330.11646739297694889No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA34390.11024810471999098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCAGC3400.088.999781
GCTGCTT600.088.999783
GCTGCGT301.9390427E-888.999783
GCTGCAT301.9390427E-888.999783
GCTGCAG301.9390427E-888.999783
GCTGCAC158.578123E-488.999783
GCTGCAA301.9390427E-888.999783
AGCTGCC1300.088.999782
GCAGCCT158.578123E-488.999783
GCAGCCG301.9390427E-888.999783
AGCAGCT550.088.999782
AGCAGCC800.088.999782
AGCTGCA750.088.999772
CAGCTGC3650.086.5614241
GCTGCCC1000.084.549783
AGCAGCG750.083.066452
CAGCGTA72800.082.764911
CTGCAGC1350.082.40721
CAGCCCT168550.081.8713461
GCAGCCA501.8189894E-1280.099793
Produced by FastQC (version 0.11.5)