FastQCFastQC Report
Tue 7 Mar 2017
1NF_17A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_17A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3119328
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG127000.082.233318
CAGCTGC850.081.033971
CACCGGC126100.080.698539
CTTCACC142300.076.228356
CCTTCAC159050.071.594325
TTCACCG154200.069.4324347
AACGGGC10550.066.736015
CGGGCCC10550.066.736017
GTGTGGC44700.066.048757
CGCTGCA652.5465852E-1065.210781
CGTAGTG25000.064.961734
ACGGGCC10950.064.2981646
GGGCCCG11350.062.0321548
GCCTTCA45600.061.863014
GGCCCGA11400.061.760089
ACCTTCA63000.061.3914834
CAGCGTA12150.061.0512351
AGGCTGC950.059.290632
CGGGGCA11750.059.12148394
GCAGCCT1000.058.2821271
Produced by FastQC (version 0.11.5)