##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1NF_17A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3119328
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.790597205551965	12.0	12.0	12.0	2.0	27.0
2	14.645932072548959	12.0	12.0	12.0	12.0	22.0
3	13.70841444054617	12.0	12.0	12.0	12.0	22.0
4	13.790121141476625	12.0	12.0	12.0	12.0	22.0
5	22.442863014085084	22.0	22.0	22.0	22.0	27.0
6	26.8012328296351	27.0	22.0	32.0	22.0	32.0
7	34.97325449584013	37.0	37.0	37.0	27.0	41.0
8	37.59664004554827	41.0	37.0	41.0	27.0	41.0
9	38.52247503308405	41.0	41.0	41.0	32.0	41.0
10-11	38.8754093830466	41.0	41.0	41.0	37.0	41.0
12-13	39.05456271350752	41.0	41.0	41.0	37.0	41.0
14-15	39.01387927143282	41.0	41.0	41.0	37.0	41.0
16-17	38.91560505980775	41.0	41.0	41.0	37.0	41.0
18-19	38.858937726330794	41.0	41.0	41.0	37.0	41.0
20-21	38.87844849916392	41.0	41.0	41.0	37.0	41.0
22-23	38.85744269278511	41.0	41.0	41.0	37.0	41.0
24-25	38.88207812708379	41.0	41.0	41.0	37.0	41.0
26-27	38.84602757388771	41.0	41.0	41.0	37.0	41.0
28-29	38.79463060633572	41.0	41.0	41.0	34.5	41.0
30-31	38.671992332964024	41.0	41.0	41.0	32.0	41.0
32-33	38.71278092589173	41.0	41.0	41.0	32.0	41.0
34-35	38.68811856271607	41.0	41.0	41.0	32.0	41.0
36-37	38.61175195426708	41.0	41.0	41.0	32.0	41.0
38-39	38.52010849772772	41.0	41.0	41.0	32.0	41.0
40-41	38.55747215425887	41.0	41.0	41.0	32.0	41.0
42-43	38.30026467239098	41.0	41.0	41.0	32.0	41.0
44-45	38.41497671934468	41.0	41.0	41.0	32.0	41.0
46-47	38.426531451646	41.0	41.0	41.0	32.0	41.0
48-49	38.37150549733789	41.0	41.0	41.0	32.0	41.0
50-51	38.26670552118918	41.0	41.0	41.0	32.0	41.0
52-53	38.23311495296423	41.0	41.0	41.0	32.0	41.0
54-55	38.10371079924907	41.0	37.0	41.0	32.0	41.0
56-57	37.880135240667215	41.0	37.0	41.0	29.5	41.0
58-59	37.87986002113276	41.0	37.0	41.0	29.5	41.0
60-61	37.875245886293456	41.0	37.0	41.0	29.5	41.0
62-63	37.86538029985945	41.0	37.0	41.0	29.5	41.0
64-65	37.85085265159675	41.0	37.0	41.0	29.5	41.0
66-67	37.71731074769951	41.0	37.0	41.0	27.0	41.0
68-69	37.74455860364797	41.0	37.0	41.0	27.0	41.0
70-71	37.721831112342144	41.0	37.0	41.0	27.0	41.0
72-73	37.55849785594846	41.0	37.0	41.0	27.0	41.0
74-75	37.48875446891125	41.0	37.0	41.0	27.0	41.0
76-77	36.438729912340094	39.0	34.5	41.0	24.5	41.0
78-79	36.941594471629784	41.0	37.0	41.0	27.0	41.0
80-81	37.59785601257707	41.0	37.0	41.0	27.0	41.0
82-83	37.78060867597124	41.0	37.0	41.0	27.0	41.0
84-85	37.68972099118785	41.0	37.0	41.0	27.0	41.0
86-87	37.5459246350496	41.0	37.0	41.0	27.0	41.0
88-89	37.4439106756327	41.0	37.0	41.0	27.0	41.0
90-91	37.27847616537921	41.0	37.0	41.0	27.0	41.0
92-93	37.189487447296344	41.0	37.0	41.0	27.0	41.0
94-95	37.02598732803989	41.0	37.0	41.0	27.0	41.0
96-97	36.96565478205562	41.0	37.0	41.0	27.0	41.0
98-99	36.8504453523323	41.0	37.0	41.0	27.0	41.0
100	36.69727838816566	41.0	37.0	41.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.238053498405515
1101	2	-0.4863610297570027
1101	3	-0.042382532382468696
1101	4	-0.055467818130717106
1101	5	-1.0677381310486282
1101	6	-0.8470050297316298
1101	7	-0.33950320662273015
1101	8	-5.4120115251521383E-5
1101	9	-0.111689491174495
1101	10-11	-0.03597369125120764
1101	12-13	-0.043977262418096075
1101	14-15	0.021800119802144025
1101	16-17	-0.08914206035166927
1101	18-19	0.08771831246984618
1101	20-21	0.04501453895312579
1101	22-23	0.0982071818610919
1101	24-25	0.03435532850249956
1101	26-27	-0.016691786938757502
1101	28-29	0.031003377306710433
1101	30-31	-0.08884429039769515
1101	32-33	-0.045126961276167776
1101	34-35	0.059666934947834704
1101	36-37	0.059859266864130234
1101	38-39	-0.01047012419993365
1101	40-41	0.023438797605841444
1101	42-43	0.2610975777959652
1101	44-45	0.08940328708229828
1101	46-47	0.19330471543741368
1101	48-49	0.18295394021174616
1101	50-51	0.16457154361342674
1101	52-53	0.06376451718492859
1101	54-55	0.024788142983588557
1101	56-57	0.31994628448317997
1101	58-59	0.06420803135358
1101	60-61	0.175887484013856
1101	62-63	0.10941767842982131
1101	64-65	0.1411677643738969
1101	66-67	0.003867825654403134
1101	68-69	0.20300957449914847
1101	70-71	0.08383617056830417
1101	72-73	0.09094126653974399
1101	74-75	0.11704656325834861
1101	76-77	-0.1364663970823088
1101	78-79	0.18114234892226477
1101	80-81	0.2178500139452808
1101	82-83	0.04570925260458836
1101	84-85	0.2557442849813256
1101	86-87	0.14132282873351443
1101	88-89	-0.14760735253342716
1101	90-91	0.04186667980819436
1101	92-93	0.1952029332867653
1101	94-95	0.14508483894190505
1101	96-97	0.08575792794863446
1101	98-99	-0.22409543005645105
1101	100	-0.47149996807331007
1102	1	1.4910006568519485
1102	2	0.6834411892492689
1102	3	-0.023747182126280464
1102	4	0.031377855328560855
1102	5	-0.7499274115012966
1102	6	-0.04101266978847562
1102	7	0.12221867444791457
1102	8	-0.09565052694176046
1102	9	-0.13168271160186862
1102	10-11	-0.24270639356277712
1102	12-13	-0.1791489681324947
1102	14-15	-0.15958447753035188
1102	16-17	-0.2626893413519156
1102	18-19	-0.08559728967354374
1102	20-21	-0.11595057232312911
1102	22-23	-0.07027008701415838
1102	24-25	-0.04220729779962795
1102	26-27	-0.12766348222303492
1102	28-29	-0.18590924443224566
1102	30-31	-0.12342056710856042
1102	32-33	-0.02434952682290259
1102	34-35	0.11997090751663819
1102	36-37	-0.13048786029197146
1102	38-39	-0.1971862687091317
1102	40-41	-0.09092718197721439
1102	42-43	0.0909108787959454
1102	44-45	0.061805602567098106
1102	46-47	-0.23053891111243985
1102	48-49	-0.19918221185928076
1102	50-51	-0.25384738182430766
1102	52-53	-0.27825573026947126
1102	54-55	-0.12867960841469994
1102	56-57	-0.15950952443716915
1102	58-59	-0.053229485758322426
1102	60-61	-0.12773456784987047
1102	62-63	-0.28522157008062266
1102	64-65	-0.36217176183827604
1102	66-67	-0.3823851567056664
1102	68-69	-0.23241154126205288
1102	70-71	-0.42112360831332296
1102	72-73	-0.19447430822460632
1102	74-75	-0.4034828301002804
1102	76-77	-0.1812660737488443
1102	78-79	-0.32739600129480806
1102	80-81	-0.22730428652015888
1102	82-83	-0.2888717828447511
1102	84-85	-0.33032610523522976
1102	86-87	-0.2040609993782425
1102	88-89	-0.36750057426456095
1102	90-91	-0.3862601373128314
1102	92-93	-0.23203784584257647
1102	94-95	-0.212909450571928
1102	96-97	-0.12072979711361853
1102	98-99	-0.3712016607444468
1102	100	-0.35521895478795074
1103	1	1.4006616201935476
1103	2	0.7230787883432654
1103	3	0.34688936608671206
1103	4	1.0784205945570307
1103	5	2.8554800344497515
1103	6	-0.2473726424451641
1103	7	-0.5859082066800951
1103	8	-0.17458985131709426
1103	9	-0.18007425149963296
1103	10-11	-0.32400438464559755
1103	12-13	-0.3355737281133173
1103	14-15	-0.4468378010690728
1103	16-17	-0.39036118080178994
1103	18-19	-0.42086669271191113
1103	20-21	-0.3391272937784464
1103	22-23	-0.21699552139531164
1103	24-25	-0.3374299723428038
1103	26-27	-0.34265210618108455
1103	28-29	-0.473947361824834
1103	30-31	-0.2704512671177426
1103	32-33	-0.41271331529994626
1103	34-35	-0.48326539787142764
1103	36-37	-0.4156365650556282
1103	38-39	-0.36515663998640946
1103	40-41	-0.5037226274793412
1103	42-43	-0.29740373253112296
1103	44-45	-0.7058501165721935
1103	46-47	-0.6777067357887532
1103	48-49	-0.5458219433234746
1103	50-51	-0.4253787093012775
1103	52-53	-0.36055644548071797
1103	54-55	-0.2868939269234758
1103	56-57	-0.34396342340976105
1103	58-59	-0.43348414585443606
1103	60-61	-0.4266936855179324
1103	62-63	-0.4896505743963786
1103	64-65	-0.3982561588060278
1103	66-67	-0.5072347174782195
1103	68-69	-0.556101459946305
1103	70-71	-0.7477541573221274
1103	72-73	-0.48890892404087083
1103	74-75	-0.5541006052933639
1103	76-77	-0.6586635618371588
1103	78-79	-0.7090117053340705
1103	80-81	-0.5675281343770777
1103	82-83	-0.6172965836364881
1103	84-85	-0.5080700779735423
1103	86-87	-0.3974528963714974
1103	88-89	-0.1846050399987007
1103	90-91	-0.5956819487051987
1103	92-93	-0.47418470391242806
1103	94-95	-0.5490560841817498
1103	96-97	-0.3148599635201137
1103	98-99	-0.5065596234636729
1103	100	-0.47064813255008886
1104	1	1.759037579447483
1104	2	-0.5297763698438338
1104	3	-0.05142739490055881
1104	4	0.04221669706465292
1104	5	-1.1057265536246064
1104	6	-0.41248983436259934
1104	7	-0.3659142051755566
1104	8	-0.319880458754902
1104	9	-0.07044491809200082
1104	10-11	-0.2045699285883913
1104	12-13	-0.09806554027628067
1104	14-15	-0.22277296268700297
1104	16-17	-0.22481499812301564
1104	18-19	-0.11470571068500135
1104	20-21	-0.05900138000490074
1104	22-23	-0.00942668210418418
1104	24-25	-0.1648125441458319
1104	26-27	-0.21983939909505068
1104	28-29	-0.10014712920558821
1104	30-31	-0.25870680848741756
1104	32-33	-0.30869425505331094
1104	34-35	-0.17785115477720126
1104	36-37	-0.15739833082039212
1104	38-39	-0.07306057282511347
1104	40-41	-0.17536728054177075
1104	42-43	-0.05728158284080109
1104	44-45	-0.04988759569628343
1104	46-47	-0.330754618860702
1104	48-49	-0.2785149454611897
1104	50-51	-0.16556593829684374
1104	52-53	-0.09578685763417383
1104	54-55	-0.20440867322481182
1104	56-57	-0.18077730451826568
1104	58-59	-0.12935202653353883
1104	60-61	-0.1830126607039375
1104	62-63	-0.24242747352386118
1104	64-65	-0.16527217773897718
1104	66-67	-0.47840424381013946
1104	68-69	-0.40299621421286247
1104	70-71	-0.2895357541784307
1104	72-73	-0.09248854532711448
1104	74-75	-0.32723708363021586
1104	76-77	-0.45032312646002026
1104	78-79	-0.2445088811790299
1104	80-81	-0.26478385001998106
1104	82-83	-0.312829097612493
1104	84-85	-0.31751910141520057
1104	86-87	-0.34782333624477957
1104	88-89	-0.2521659632425397
1104	90-91	-0.31504359515562186
1104	92-93	-0.5604048814744473
1104	94-95	-0.2597632073677971
1104	96-97	-0.2796545177821912
1104	98-99	-0.4402676442387943
1104	100	-0.3191844832686783
1105	1	1.4525217309180842
1105	2	0.35659668071766504
1105	3	-0.012326460756026947
1105	4	-0.16443526132089303
1105	5	-1.2006526139929008
1105	6	0.44988452877995755
1105	7	0.0631245117921111
1105	8	-0.15171566201193798
1105	9	-0.12000737368283865
1105	10-11	-0.11811082467016121
1105	12-13	-0.09772357013176247
1105	14-15	0.04151492035347104
1105	16-17	-0.03264134093621607
1105	18-19	-0.04872751434666611
1105	20-21	-0.013476050216304714
1105	22-23	-0.11018058150288113
1105	24-25	-0.10120909700330571
1105	26-27	-0.12723004951130434
1105	28-29	-0.10260169751979475
1105	30-31	-0.19135434082459568
1105	32-33	-0.049876459667785866
1105	34-35	-0.140915249007584
1105	36-37	-0.16114200398144618
1105	38-39	-0.18823682178563672
1105	40-41	-0.11094725251638948
1105	42-43	-0.01889458636640029
1105	44-45	-0.14061689202554817
1105	46-47	-0.29518124630993015
1105	48-49	-0.34206009334567256
1105	50-51	-0.06837481480884122
1105	52-53	-0.1818460834200195
1105	54-55	-0.04379447815105664
1105	56-57	-0.09004253040637877
1105	58-59	-0.09330626044216217
1105	60-61	-0.15864787481220333
1105	62-63	-0.16046628126621698
1105	64-65	-0.23745096317371406
1105	66-67	-0.14163981872168563
1105	68-69	-0.21745106528499036
1105	70-71	-0.3345007850574788
1105	72-73	-0.27671889599911026
1105	74-75	-0.29765457935494055
1105	76-77	-0.26805160477374557
1105	78-79	-0.1440506516192528
1105	80-81	-0.28918058964376314
1105	82-83	-0.23552636821599293
1105	84-85	-0.1367953141701932
1105	86-87	-0.19078378561076192
1105	88-89	-0.27086346130445094
1105	90-91	-0.30122236702632676
1105	92-93	-0.2897361642042
1105	94-95	-0.2036023713225248
1105	96-97	-0.2768800208773996
1105	98-99	-0.21350022462916485
1105	100	-0.2920152030910472
1106	1	1.3248696698678817
1106	2	-0.684890321262337
1106	3	-0.06975717315996732
1106	4	-0.20265012087951106
1106	5	-1.1126597391625346
1106	6	-0.33121955184369867
1106	7	0.12379700815895234
1106	8	0.06811545559199317
1106	9	-0.05680250875958137
1106	10-11	0.07577682169768707
1106	12-13	-0.019480820217907535
1106	14-15	-0.027724706713236458
1106	16-17	0.019811898495291302
1106	18-19	0.10100723683619606
1106	20-21	0.0016062553758757758
1106	22-23	-0.04065826593313915
1106	24-25	-0.08904350330882238
1106	26-27	-0.0746285004676821
1106	28-29	-0.11316249841535608
1106	30-31	-0.036962391835217545
1106	32-33	-0.03801149356544187
1106	34-35	-0.062253788926064146
1106	36-37	-0.2761703346000388
1106	38-39	-0.006364356749784861
1106	40-41	-0.04941847677182665
1106	42-43	0.13840940904329102
1106	44-45	-0.10813242149414748
1106	46-47	-0.26468794895815506
1106	48-49	-0.1847760083535519
1106	50-51	-0.2731587821783634
1106	52-53	-0.14260442457077005
1106	54-55	-0.2301804311477511
1106	56-57	-0.27947083463544686
1106	58-59	-0.22987372022246433
1106	60-61	-0.14199356618470205
1106	62-63	-0.2637933974676159
1106	64-65	-0.4807587629845145
1106	66-67	-0.2908860089979868
1106	68-69	-0.3279231958258464
1106	70-71	-0.4129811457632755
1106	72-73	-0.34820656034548136
1106	74-75	-0.4289432157502375
1106	76-77	-0.3472442064568355
1106	78-79	-0.3306041675361513
1106	80-81	-0.3059173655123857
1106	82-83	-0.21418502901108383
1106	84-85	-0.40858302785234457
1106	86-87	-0.4625036855934397
1106	88-89	-0.44340897993600237
1106	90-91	-0.6521614159155291
1106	92-93	-0.5434721602152806
1106	94-95	-0.43523142986072827
1106	96-97	-0.27007105233165163
1106	98-99	-0.4206580657458687
1106	100	-0.45931278248367846
1107	1	1.3649717478846526
1107	2	-0.5645840872120278
1107	3	-0.1171505380888771
1107	4	-0.23546091198414132
1107	5	-1.2311431514848081
1107	6	-0.4055906783475258
1107	7	-0.32498170128326365
1107	8	-0.2629089702191578
1107	9	-0.23215795899654523
1107	10-11	-0.11871825668364266
1107	12-13	-0.26027557049133065
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2223	3	4.8746993570070174E-4
2223	4	7.665090554223042E-4
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2224	84-85	0.01578858087945889
2224	86-87	0.3836428949923132
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2224	100	0.43185955229055395
2225	1	1.170815361963097
2225	2	1.83447724147109
2225	3	0.7457646221681333
2225	4	0.7412178426389033
2225	5	3.0741809944252907
2225	6	-0.19551882524324782
2225	7	0.09973130745462555
2225	8	0.24577867804475773
2225	9	0.22041966026335302
2225	10-11	0.12056171104919855
2225	12-13	0.13454401338657362
2225	14-15	0.15094450635832146
2225	16-17	0.052177627665244586
2225	18-19	0.15651048824451408
2225	20-21	0.1845831701868974
2225	22-23	0.19048941485206683
2225	24-25	0.20136723613062912
2225	26-27	0.1674789041446374
2225	28-29	0.11852443106862154
2225	30-31	0.10046958966697161
2225	32-33	0.17920551941988805
2225	34-35	0.1643782325563805
2225	36-37	0.11898276736047109
2225	38-39	0.20090711033286368
2225	40-41	0.13231586703658849
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2225	44-45	0.1321110283665945
2225	46-47	0.16365537659964247
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2225	54-55	0.16892340602625922
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2225	60-61	0.3579025532813205
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2225	64-65	0.3506187649443504
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2225	74-75	0.38054859622364035
2225	76-77	0.4672946537051601
2225	78-79	0.36024323693730764
2225	80-81	0.22083043102166755
2225	82-83	0.1594424674457784
2225	84-85	0.18320773762668097
2225	86-87	0.33972343183702236
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2225	90-91	0.33844047418619994
2225	92-93	0.4375421329864082
2225	94-95	0.5865721510440665
2225	96-97	0.616886360186335
2225	98-99	0.5856038476025631
2225	100	0.4756766048032617
2226	1	1.335976104402505
2226	2	1.497103564070441
2226	3	0.7468884749900635
2226	4	1.0442166597982663
2226	5	3.104345101354909
2226	6	-0.325249223939192
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2226	9	0.2541773974342121
2226	10-11	0.09413542679835274
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2226	16-17	0.046961511217389784
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2226	74-75	0.38088093280425994
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2226	80-81	0.22693110767231417
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2226	84-85	0.23675447319727994
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2226	90-91	0.3600595085132525
2226	92-93	0.2842918529549294
2226	94-95	0.35426701774188274
2226	96-97	0.40837432847119004
2226	98-99	0.43861970568636366
2226	100	0.561136069764494
2227	1	1.2722991523442246
2227	2	1.5653384601347415
2227	3	0.6155149616764426
2227	4	0.0850363762019537
2227	5	3.0977156552336567
2227	6	0.0410487409250635
2227	7	0.2617962785088963
2227	8	0.3314295814111432
2227	9	0.11267820276262341
2227	10-11	0.17155231992368414
2227	12-13	0.11197395314415814
2227	14-15	0.20180484887148253
2227	16-17	0.18980641504087004
2227	18-19	0.011618066547896433
2227	20-21	4.902088226970136E-4
2227	22-23	0.17987566116520526
2227	24-25	0.25516910613379906
2227	26-27	0.09705888762625392
2227	28-29	0.27115440484448783
2227	30-31	0.14288702757944094
2227	32-33	0.10234252096317675
2227	34-35	0.12313871601158866
2227	36-37	0.024414206594364884
2227	38-39	0.11487420792767722
2227	40-41	0.059156788299823404
2227	42-43	0.2611675566393217
2227	44-45	0.23947453356460358
2227	46-47	0.2682070425637946
2227	48-49	0.20895198597816744
2227	50-51	0.062198445912621025
2227	52-53	0.2940760795525037
2227	54-55	0.19993328764188334
2227	56-57	0.46922422182249335
2227	58-59	0.4238192827098217
2227	60-61	0.22445609975731173
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2227	64-65	0.3115998145289396
2227	66-67	0.28808088316330327
2227	68-69	0.18052806262499388
2227	70-71	0.2548085841580132
2227	72-73	0.2873714918121095
2227	74-75	0.22094102171006114
2227	76-77	0.41020599896741317
2227	78-79	0.3262686393916425
2227	80-81	0.2968955801323361
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2227	84-85	0.23070460012153404
2227	86-87	0.21342852446809246
2227	88-89	0.2701219749918806
2227	90-91	0.277420575935281
2227	92-93	0.22728034256391538
2227	94-95	0.39810505338501656
2227	96-97	0.29204669765071856
2227	98-99	0.4240420849676596
2227	100	0.4899013317481362
2228	1	1.4253424086500601
2228	2	1.5075713400627127
2228	3	-0.16581864439724292
2228	4	-0.0017754262037730228
2228	5	3.2329634950803836
2228	6	-0.03743679608791339
2228	7	0.3996218203797426
2228	8	0.3613462346934071
2228	9	0.26219103389282594
2228	10-11	0.13720117342708704
2228	12-13	0.22859248903539253
2228	14-15	0.25662807987921354
2228	16-17	0.2125864331757299
2228	18-19	0.20497762439264022
2228	20-21	0.12542513897777496
2228	22-23	0.37781497703100797
2228	24-25	0.32917557064059366
2228	26-27	0.29343557194334124
2228	28-29	0.18564603724772155
2228	30-31	-0.015531281314039802
2228	32-33	0.19669599322510578
2228	34-35	0.21182781608177237
2228	36-37	0.24076508841733357
2228	38-39	0.3096094190341887
2228	40-41	0.26875989376971887
2228	42-43	0.4468822117248834
2228	44-45	0.28237560612962653
2228	46-47	0.26560316612690116
2228	48-49	0.3877375543622321
2228	50-51	0.258076797431535
2228	52-53	0.3271623204852361
2228	54-55	0.12707983467641526
2228	56-57	0.5360921406408039
2228	58-59	0.6544780793058464
2228	60-61	0.5365377629062493
2228	62-63	0.26580177246391656
2228	64-65	0.3887310326555422
2228	66-67	0.44840139043998306
2228	68-69	0.5673705992682159
2228	70-71	0.5010941420341055
2228	72-73	0.3926961825369091
2228	74-75	0.5004494242060389
2228	76-77	0.369777039189529
2228	78-79	0.5672602153065469
2228	80-81	0.4889891497732748
2228	82-83	0.3599113429957299
2228	84-85	0.45918537988722363
2228	86-87	0.5711111731505412
2228	88-89	0.5570511737784543
2228	90-91	0.4081350202244778
2228	92-93	0.5254485539879568
2228	94-95	0.5980378914976043
2228	96-97	0.5978819104257624
2228	98-99	0.6764146267222202
2228	100	0.7009012338010621
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	16.0
13	50.0
14	157.0
15	565.0
16	2120.0
17	5265.0
18	8642.0
19	11106.0
20	12805.0
21	14987.0
22	17231.0
23	19523.0
24	22127.0
25	25111.0
26	28118.0
27	31970.0
28	35992.0
29	40678.0
30	46825.0
31	53959.0
32	62759.0
33	74921.0
34	92153.0
35	119019.0
36	166530.0
37	276305.0
38	676504.0
39	1272346.0
40	1544.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.631402316959246	19.596067440722454	18.83984220864496	35.932688033673344
2	18.253834414834955	52.01255482315524	12.348931749980764	17.384679012029032
3	42.1808799844069	15.476025605515034	23.216442772289415	19.12665163778865
4	17.110287856871736	16.891875429579702	21.983388729880282	44.014447983668276
5	16.525290062474994	33.8682562398055	27.181495501595215	22.424958196124294
6	17.374767898726905	28.86073538916074	28.954537644005374	24.809959068106977
7	19.954714605197015	25.919428800049243	36.428679510458664	17.697177084295078
8	21.734777490536423	28.61722140153264	32.62455887934837	17.023442228582567
9	20.968682998389397	28.38277988079484	26.588803742344503	24.05973337847126
10-11	19.480878573846674	30.654214625714253	28.374060053960342	21.490846746478727
12-13	23.92922449963582	26.601979657156928	27.529038305686353	21.9397575375209
14-15	21.851725756316746	27.642299879974146	28.4899023122929	22.016072051416202
16-17	20.673683562613483	30.13714812934068	28.216494065388442	20.972674242657394
18-19	21.379765128899557	29.646561695339507	29.640935483540044	19.33273769222089
20-21	22.578116183998606	29.111638788867346	29.976200002051723	18.334045025082325
22-23	21.67532558294607	30.5856902512336	28.583287810707947	19.155696355112383
24-25	22.364961299356782	28.33438804768206	26.423848341694107	22.876802311267042
26-27	19.945177294596785	26.717132664471322	30.15452366663589	23.183166374296
28-29	19.179051844632806	28.37007745277817	30.659887411358888	21.79098329123014
30-31	19.87850311477039	30.210435362502846	28.47945341630838	21.43160810641838
32-33	20.429720760368898	30.16096736220109	28.870048292452733	20.539263584977277
34-35	21.163901327465403	29.223217308343337	30.265108382318246	19.347772981873018
36-37	21.711214723171143	28.266104109603095	28.782930169574982	21.239750997650773
38-39	21.636192647925583	29.186981744120832	29.133764616476824	20.043060991476764
40-41	20.85852752133431	29.996191417802432	29.8007290806089	19.34455198025436
42-43	20.420078129094936	29.99085218610632	29.532259308064933	20.056810376733807
44-45	20.35779821807774	30.20362077986028	28.93070238205152	20.507878620010462
46-47	19.764520959067674	28.42510487668784	28.829800415707773	22.980573748536713
48-49	20.663511712622626	28.083141010865486	30.511629591895943	20.741717684615946
50-51	21.70783258445409	31.079466474830475	28.177286902820093	19.035414037895343
52-53	20.298369180166	30.586319322666377	28.271481651273206	20.84382984589442
54-55	22.14909165648881	28.778843357438472	28.13379874107761	20.938266244995106
56-57	21.34722523023089	28.81127968249963	29.612741438450318	20.22875364881916
58-59	21.278211182800803	28.225787575713174	28.86745180825069	21.62854943323534
60-61	19.47224783318437	30.479183951394596	29.800591410190158	20.247976805230877
62-63	20.480163132346245	31.4289951073121	27.309406400892613	20.781435359449045
64-65	19.75031773706134	30.341601202439822	28.742873657421665	21.165207403077172
66-67	20.07874773028037	30.547605125206456	29.410629468911253	19.963017675601925
68-69	20.796706854809756	29.951931954574835	29.332663958391038	19.91869723222438
70-71	19.35113909149663	31.27999043383703	29.345182680372183	20.02368779429416
72-73	20.092132023307585	30.82918820976826	28.817232429548927	20.26144733737523
74-75	19.94827090963182	29.39367068804563	30.38128725161317	20.276771150709383
76-77	19.431508966033707	30.72700273905149	28.781134911109056	21.060353383805744
78-79	19.23449537849178	29.695803070404907	28.696918054144994	22.37278349695832
80-81	20.477039408871732	29.820893828564728	28.49861973255094	21.2034470300126
82-83	19.808913971214313	28.817056109521026	30.108039295643163	21.265990623621498
84-85	20.13127506950215	29.52743347285056	28.19700268775839	22.1442887698889
86-87	21.375902117379127	28.800433939617765	29.52736935647678	20.29629458652633
88-89	20.778401630094688	28.024497584095037	30.58442395285138	20.612676832958893
90-91	20.974245093815078	28.022045132797835	30.897985078837493	20.105724694549597
92-93	21.06298215513085	28.15943049272151	31.595731516531767	19.181855835615877
94-95	20.87573028549739	28.559019763231053	31.02568886631992	19.539561084951632
96-97	20.778353542814347	28.344165794683985	31.142380666605114	19.73509999589655
98-99	21.777446937289056	28.29603042706634	30.524923957980693	19.401598677663905
100	21.758244083341026	30.202819325187992	29.204559443572464	18.834377147898522
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	12.5
2	23.0
3	22.0
4	25.5
5	40.0
6	47.5
7	55.5
8	73.0
9	80.0
10	86.5
11	111.0
12	133.0
13	150.5
14	168.0
15	230.5
16	316.5
17	408.0
18	571.0
19	846.0
20	1386.5
21	2217.5
22	3443.5
23	5262.0
24	7985.0
25	11901.5
26	17188.5
27	23391.0
28	30256.0
29	38058.5
30	47325.0
31	57873.5
32	69616.0
33	83040.0
34	97746.0
35	113597.5
36	129432.5
37	141023.5
38	147512.0
39	151488.5
40	154090.5
41	154385.5
42	151688.5
43	147971.5
44	144906.0
45	140238.5
46	130076.5
47	117108.5
48	107475.0
49	102164.5
50	97740.5
51	90851.5
52	79571.5
53	66206.5
54	54552.0
55	44483.0
56	34890.0
57	26157.0
58	18919.5
59	13652.5
60	10578.5
61	8832.5
62	7206.0
63	5474.5
64	4114.5
65	3493.5
66	3278.0
67	2805.0
68	2153.5
69	2054.0
70	2527.5
71	2438.5
72	1522.0
73	735.0
74	396.5
75	255.0
76	167.0
77	132.5
78	100.0
79	68.0
80	51.0
81	42.5
82	41.0
83	36.0
84	26.5
85	23.0
86	20.5
87	24.0
88	24.5
89	20.5
90	18.0
91	13.0
92	12.5
93	13.0
94	13.0
95	15.5
96	18.0
97	18.0
98	45.0
99	106.0
100	104.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.361774074416028
2	0.006668102873439408
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	2.564654951322849E-4
30-31	0.010194503431508324
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0010098328870833718
40-41	0.00182731665281753
42-43	1.1220365412037464E-4
44-45	0.0
46-47	0.0022280439889617253
48-49	3.205818689153561E-5
50-51	0.0
52-53	0.0012182111018783533
54-55	0.0201645995547759
56-57	0.01088375444967634
58-59	0.015548220642394773
60-61	1.2823274756614244E-4
62-63	0.01243857651391582
64-65	1.1220365412037464E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.6029093445767806E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3119328.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.67914377420152
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.33117303371954	62.017965933389696
2	8.937631934318205	12.991588727104153
3	2.6192139366885194	5.7108667883993185
4	1.1054904686822413	3.2138440285746417
5	0.5871591876195135	2.133711350767099
6	0.3486726171134368	1.5204736361588753
7	0.22568389894245705	1.1481758777132842
8	0.15726610927361634	0.9143972933365158
9	0.1132457519410262	0.7407543858425464
>10	0.5376135154274742	6.615081559699668
>50	0.02584429218969867	1.2903546853736942
>100	0.010152237155606664	1.2763800576767645
>500	7.632256732564292E-4	0.340785191456208
>1k	8.979125543016483E-5	0.08562048450754405
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	3.205818689153561E-5	0.0	0.0	0.0	0.0
10-11	6.411637378307122E-5	0.0	0.0	0.0	0.0
12-13	9.617456067460684E-5	0.0	0.0	0.0	0.0
14-15	9.617456067460684E-5	0.0	0.0	0.0	0.0
16-17	9.617456067460684E-5	0.0	0.0	0.0	0.0
18-19	9.617456067460684E-5	0.0	0.0	0.0	0.0
20-21	9.617456067460684E-5	0.0	0.0	0.0	0.0
22-23	9.617456067460684E-5	1.6029093445767805E-5	0.0	0.0	0.0
24-25	9.617456067460684E-5	3.205818689153561E-5	0.0	0.0	0.0
26-27	9.617456067460684E-5	3.205818689153561E-5	0.0	0.0	0.0
28-29	9.617456067460684E-5	3.205818689153561E-5	0.0	0.0	0.0
30-31	9.617456067460684E-5	3.205818689153561E-5	0.0	0.0	0.0
32-33	9.617456067460684E-5	6.411637378307122E-5	0.0	0.0	0.0
34-35	9.617456067460684E-5	6.411637378307122E-5	0.0	0.0	0.0
36-37	9.617456067460684E-5	6.411637378307122E-5	0.0	0.0	0.0
38-39	9.617456067460684E-5	6.411637378307122E-5	0.0	0.0	0.0
40-41	9.617456067460684E-5	6.411637378307122E-5	0.0	0.0	0.0
42-43	9.617456067460684E-5	6.411637378307122E-5	0.0	0.0	0.0
44-45	9.617456067460684E-5	6.411637378307122E-5	0.0	0.0	0.0
46-47	9.617456067460684E-5	6.411637378307122E-5	0.0	0.0	0.0
48-49	9.617456067460684E-5	6.411637378307122E-5	0.0	0.0	0.0
50-51	9.617456067460684E-5	6.411637378307122E-5	0.0	0.0	0.0
52-53	1.1220365412037464E-4	6.411637378307122E-5	0.0	0.0	0.0
54-55	1.4426184101191025E-4	9.617456067460684E-5	0.0	3.5264005580689175E-4	0.0
56-57	2.083782147949815E-4	9.617456067460684E-5	0.0	7.052801116137835E-4	0.0
58-59	2.885236820238205E-4	9.617456067460684E-5	0.0	7.052801116137835E-4	0.0
60-61	3.3661096236112394E-4	9.617456067460684E-5	0.0	7.052801116137835E-4	0.0
62-63	4.4881461648149857E-4	9.617456067460684E-5	0.0	7.052801116137835E-4	0.0
64-65	5.289600837103376E-4	9.617456067460684E-5	0.0	7.052801116137835E-4	0.0
66-67	6.091055509391766E-4	9.617456067460684E-5	0.0	7.052801116137835E-4	0.0
68-69	8.014546722883904E-4	9.617456067460684E-5	0.0	7.052801116137835E-4	0.0
70-71	8.335128591799259E-4	9.617456067460684E-5	0.0	7.052801116137835E-4	0.0
72-73	8.495419526256937E-4	9.617456067460684E-5	0.0	7.052801116137835E-4	0.0
74-75	9.777747001918362E-4	9.617456067460684E-5	0.0	7.052801116137835E-4	0.0
76-77	0.001138065634649514	9.617456067460684E-5	0.0	7.052801116137835E-4	0.0
78-79	0.0013304147559987278	9.617456067460684E-5	0.0	7.052801116137835E-4	0.0
80-81	0.0015548220642394772	9.617456067460684E-5	0.0	7.052801116137835E-4	0.0
82-83	0.0016830548118056195	9.617456067460684E-5	0.0	7.052801116137835E-4	0.0
84-85	0.0017952584659259943	9.617456067460684E-5	0.0	7.052801116137835E-4	0.0
86-87	0.0019234912134921368	1.2823274756614244E-4	0.0	7.052801116137835E-4	0.0
88	0.0019234912134921368	1.2823274756614244E-4	0.0	7.052801116137835E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	12700	0.0	82.23331	8
CAGCTGC	85	0.0	81.03397	1
CACCGGC	12610	0.0	80.69853	9
CTTCACC	14230	0.0	76.22835	6
CCTTCAC	15905	0.0	71.59432	5
TTCACCG	15420	0.0	69.432434	7
AACGGGC	1055	0.0	66.73601	5
CGGGCCC	1055	0.0	66.73601	7
GTGTGGC	4470	0.0	66.04875	7
CGCTGCA	65	2.5465852E-10	65.21078	1
CGTAGTG	2500	0.0	64.96173	4
ACGGGCC	1095	0.0	64.298164	6
GGGCCCG	1135	0.0	62.032154	8
GCCTTCA	4560	0.0	61.86301	4
GGCCCGA	1140	0.0	61.76008	9
ACCTTCA	6300	0.0	61.391483	4
CAGCGTA	1215	0.0	61.051235	1
AGGCTGC	95	0.0	59.29063	2
CGGGGCA	1175	0.0	59.121483	94
GCAGCCT	100	0.0	58.282127	1
>>END_MODULE