FastQCFastQC Report
Mon 6 Mar 2017
1NF_18A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_18A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1750070
Sequences flagged as poor quality0
Sequence length95
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA112740.6442028033164388No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA40910.23376207808830504No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA40320.23039078436862523No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG39220.22410532150142567No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG37430.21387715919934633No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT36520.2086773671910266No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG35040.2002205626060672No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG29990.17136457398846905No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT29890.17079316827326907No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT27060.1546223865331101No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA26200.14970829738239042No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA25940.1482226425228705No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC25820.14753695566463057No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA24750.14142291451199096No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA22020.12582353848703196No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA21470.12268080705343215No Hit
CAGCACTTTGAACTTTGTTCACCATTTCTTCGTAGTCGTTGATACGTTGT20430.11673818761535253No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC19830.11330975332415276No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC19130.10930991331775301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTGC450.089.01
GCTGCCC202.4110705E-589.03
GCTGCAT202.4110705E-589.03
GCAGCTA256.815262E-789.03
AGCAGCC202.4110705E-589.02
GCTGCAA158.577405E-488.999993
TGCTGCA158.577405E-488.999992
GCAGCTC158.577405E-488.999993
GCAGCCA158.577405E-488.999993
GCAGCAC158.577405E-488.999993
CAGCAGC2050.086.829271
CAGCTGC2050.086.829271
CAGCCCT111100.083.232221
AGCAGCG750.083.0666662
AGCTGCC700.082.642862
AGCAGCA700.082.642862
CAGCGTA35650.081.884991
AGCCCTT95550.080.989532
AGCTGCT501.8189894E-1280.12
AGCTGCA501.8189894E-1280.12
Produced by FastQC (version 0.11.5)