##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1NF_18A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1750070
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.859737039089865	12.0	12.0	12.0	2.0	32.0
2	14.588607884256058	12.0	12.0	12.0	12.0	22.0
3	13.72917369019525	12.0	12.0	12.0	12.0	22.0
4	13.77521184866891	12.0	12.0	12.0	12.0	22.0
5	22.422637380219076	22.0	22.0	22.0	22.0	27.0
6	26.82880970475467	27.0	22.0	32.0	22.0	32.0
7	34.93294439650985	37.0	37.0	37.0	27.0	41.0
8	37.53985954847521	41.0	37.0	41.0	27.0	41.0
9	38.500237133371805	41.0	37.0	41.0	32.0	41.0
10-11	38.87414274857577	41.0	41.0	41.0	37.0	41.0
12-13	39.04922717377019	41.0	41.0	41.0	37.0	41.0
14-15	38.9958330238219	41.0	41.0	41.0	37.0	41.0
16-17	38.89145891307205	41.0	41.0	41.0	37.0	41.0
18-19	38.83005679772809	41.0	41.0	41.0	37.0	41.0
20-21	38.85982674978715	41.0	41.0	41.0	37.0	41.0
22-23	38.83848588913587	41.0	41.0	41.0	34.5	41.0
24-25	38.84511562394647	41.0	41.0	41.0	37.0	41.0
26-27	38.813895158479376	41.0	41.0	41.0	37.0	41.0
28-29	38.7666536195695	41.0	41.0	41.0	34.5	41.0
30-31	38.65609518476404	41.0	41.0	41.0	32.0	41.0
32-33	38.69780380213363	41.0	41.0	41.0	32.0	41.0
34-35	38.66981349317456	41.0	41.0	41.0	32.0	41.0
36-37	38.59261915237676	41.0	41.0	41.0	32.0	41.0
38-39	38.49703240441811	41.0	41.0	41.0	32.0	41.0
40-41	38.53808076248379	41.0	41.0	41.0	32.0	41.0
42-43	38.28457318850103	41.0	41.0	41.0	32.0	41.0
44-45	38.40517807859114	41.0	41.0	41.0	32.0	41.0
46-47	38.39372425102996	41.0	41.0	41.0	32.0	41.0
48-49	38.355312930339934	41.0	41.0	41.0	32.0	41.0
50-51	38.239763552315054	41.0	41.0	41.0	32.0	41.0
52-53	38.21905866622478	41.0	41.0	41.0	32.0	41.0
54-55	38.07775460410156	41.0	37.0	41.0	32.0	41.0
56-57	37.867245881593306	41.0	37.0	41.0	29.5	41.0
58-59	37.85390927220054	41.0	37.0	41.0	29.5	41.0
60-61	37.85039227002349	41.0	37.0	41.0	29.5	41.0
62-63	37.856310319015805	41.0	37.0	41.0	29.5	41.0
64-65	37.84033581513882	41.0	37.0	41.0	29.5	41.0
66-67	37.72506585450868	41.0	37.0	41.0	27.0	41.0
68-69	37.73732364991115	41.0	37.0	41.0	27.0	41.0
70-71	37.73203986126269	41.0	37.0	41.0	27.0	41.0
72-73	37.56987806202038	41.0	37.0	41.0	27.0	41.0
74-75	37.4839689269572	41.0	37.0	41.0	27.0	41.0
76-77	36.4241364631129	39.0	34.5	41.0	24.5	41.0
78-79	36.94481706446028	41.0	37.0	41.0	27.0	41.0
80-81	37.616019644928485	41.0	37.0	41.0	27.0	41.0
82-83	37.79115549663727	41.0	37.0	41.0	27.0	41.0
84-85	37.716884753181304	41.0	37.0	41.0	27.0	41.0
86-87	37.58030850194564	41.0	37.0	41.0	27.0	41.0
88-89	37.48097704663242	41.0	37.0	41.0	27.0	41.0
90-91	37.308348237499075	41.0	37.0	41.0	27.0	41.0
92-93	37.21395601318804	41.0	37.0	41.0	27.0	41.0
94-95	37.051768214985685	41.0	37.0	41.0	27.0	41.0
96-97	37.00527950310559	41.0	37.0	41.0	27.0	41.0
98-99	36.89110464152863	41.0	37.0	41.0	27.0	41.0
100	36.742554297828086	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.3780418576638507
1101	2	-0.6823915911125695
1101	3	-0.025084377887818476
1101	4	-0.035174505946127255
1101	5	-1.1619692475091306
1101	6	-0.9092018012987744
1101	7	-0.4457617357406676
1101	8	-0.18478410577663595
1101	9	0.14007341563240772
1101	10-11	0.04985690998799441
1101	12-13	0.2485357511830344
1101	14-15	0.23219087360779156
1101	16-17	0.01814120094646654
1101	18-19	0.10332816884962881
1101	20-21	0.1478566893891582
1101	22-23	0.011527532558417874
1101	24-25	0.09780765679301595
1101	26-27	-0.04512807577884104
1101	28-29	0.04129905997862693
1101	30-31	-0.11727612841986002
1101	32-33	0.05740797088352423
1101	34-35	0.27311998739600085
1101	36-37	0.08962892123700783
1101	38-39	0.1811389679143076
1101	40-41	0.15298044700605118
1101	42-43	0.285773908926501
1101	44-45	0.1692161652522728
1101	46-47	0.2303926350014862
1101	48-49	0.2947097960346312
1101	50-51	0.19557411962173177
1101	52-53	0.24028003736034975
1101	54-55	0.3647884774716701
1101	56-57	0.24188512016034736
1101	58-59	0.3729789096437841
1101	60-61	0.16352486510901088
1101	62-63	0.03222055120465228
1101	64-65	0.00830691614842749
1101	66-67	0.1289591329132449
1101	68-69	0.13161023536049044
1101	70-71	0.11461821978453912
1101	72-73	0.19319223775782035
1101	74-75	0.21691526952901086
1101	76-77	-0.11149792803959713
1101	78-79	0.09183639120277576
1101	80-81	0.11753769677652315
1101	82-83	-0.08099901700690992
1101	84-85	-0.005370739144993308
1101	86-87	-0.1414619955469547
1101	88-89	-0.18638914499209847
1101	90-91	-0.004320303363449796
1101	92-93	0.04433300945593999
1101	94-95	0.12296024587743659
1101	96-97	0.05095816835309819
1101	98-99	-0.11658629646750995
1101	100	-0.43061785148548637
1102	1	1.6895842945353081
1102	2	0.8952445531774451
1102	3	-0.017538115748992666
1102	4	-0.01794252391923301
1102	5	-0.7917184282837546
1102	6	-0.12073435096742813
1102	7	-0.1269501276660634
1102	8	-0.15747797803729924
1102	9	-0.29923494545156615
1102	10-11	-0.38185429894272716
1102	12-13	-0.21839487887272924
1102	14-15	-0.11066761642524625
1102	16-17	-0.15502858642050654
1102	18-19	-0.06241437669797989
1102	20-21	-0.0639328029237447
1102	22-23	-0.0794816766647628
1102	24-25	-0.10274989005072399
1102	26-27	-0.08912135081921946
1102	28-29	-0.0639203029864035
1102	30-31	-0.05858290921308651
1102	32-33	-0.1481023167925315
1102	34-35	0.020338484607648866
1102	36-37	-0.2632530322083966
1102	38-39	-0.13210236458152735
1102	40-41	0.08121160980756059
1102	42-43	0.17363495800026385
1102	44-45	0.10446786518646434
1102	46-47	-0.3323956725088024
1102	48-49	-0.15555549672627222
1102	50-51	-0.3399383864435279
1102	52-53	-0.22041646207134136
1102	54-55	-0.090438695552713
1102	56-57	-0.13134806162460677
1102	58-59	-0.0575717992437248
1102	60-61	-0.11435621593614087
1102	62-63	-0.09326072293175258
1102	64-65	-0.21648154756304194
1102	66-67	-0.05553316337714165
1102	68-69	-0.5399811307019746
1102	70-71	-0.4225514313218284
1102	72-73	-0.23280931926890958
1102	74-75	-0.22846271241812133
1102	76-77	0.010874277787038977
1102	78-79	-0.18183224293416345
1102	80-81	-0.29108107760913526
1102	82-83	-0.5054213218717436
1102	84-85	-0.4617122996280827
1102	86-87	-0.3088130809790286
1102	88-89	-0.2567955838044327
1102	90-91	-0.2778200657382399
1102	92-93	-0.4816181976136562
1102	94-95	-0.35210364705496033
1102	96-97	-0.347465986000266
1102	98-99	-0.29339856258116015
1102	100	-0.512941277675445
1103	1	1.8379739256641248
1103	2	0.5529552375895523
1103	3	0.3401994686336529
1103	4	0.9598756749782957
1103	5	2.9962286769805253
1103	6	-0.3003629605805358
1103	7	-0.22985062766392872
1103	8	-0.20811140829592034
1103	9	-0.11120158948809689
1103	10-11	-0.1875812172735607
1103	12-13	-0.37914312503735204
1103	14-15	-0.41912552224118826
1103	16-17	-0.45180998359644065
1103	18-19	-0.4025002431118665
1103	20-21	-0.14677318362237202
1103	22-23	-0.13385121394257027
1103	24-25	-0.22915904287244615
1103	26-27	0.039766015161283974
1103	28-29	-0.08399878258298799
1103	30-31	-0.03522733003110545
1103	32-33	-0.21600339321833673
1103	34-35	-0.0975373997822544
1103	36-37	-0.19642154984921945
1103	38-39	-0.16963380125343974
1103	40-41	-0.1216325283472699
1103	42-43	0.07355169618669066
1103	44-45	-0.2985545386461368
1103	46-47	-0.5124258602535221
1103	48-49	-0.4431588801447859
1103	50-51	-0.3543302976650864
1103	52-53	-0.22143609904983919
1103	54-55	-0.24011781733272386
1103	56-57	-0.20987377244564698
1103	58-59	-0.618037168734034
1103	60-61	-0.273713135846819
1103	62-63	-0.36685306455283495
1103	64-65	-0.26299778788652617
1103	66-67	-0.2453436253307686
1103	68-69	-0.3811743792363558
1103	70-71	-0.34055092748111093
1103	72-73	-0.2466047343183746
1103	74-75	-0.2796493000074065
1103	76-77	-0.3922461187953772
1103	78-79	-0.3140061021405671
1103	80-81	-0.06805448933667435
1103	82-83	-0.2351167544275441
1103	84-85	-0.3848422179461082
1103	86-87	-0.39383577516052526
1103	88-89	-0.13567142122613518
1103	90-91	-0.46877164152142825
1103	92-93	-0.26163621632407796
1103	94-95	-0.30459744511730236
1103	96-97	-0.25145657528041454
1103	98-99	-0.28506618996105715
1103	100	-0.2026129699397785
1104	1	1.486179786374855
1104	2	-0.5045481169531474
1104	3	-0.1378336872335506
1104	4	0.08442659910070383
1104	5	-1.03863692488936
1104	6	-0.5294068076999601
1104	7	-0.22321945422324774
1104	8	-0.2385490138017019
1104	9	0.2256421104642925
1104	10-11	0.00714457591347184
1104	12-13	0.07395603957330366
1104	14-15	-0.056382588423069535
1104	16-17	0.08520036138040155
1104	18-19	0.003349646512852189
1104	20-21	-0.14343811891216518
1104	22-23	-0.01979026947648066
1104	24-25	-0.006064639548206685
1104	26-27	-0.06515325939180627
1104	28-29	-0.09680231459181243
1104	30-31	-0.17692970634587368
1104	32-33	0.03458311069890385
1104	34-35	-0.13139663884211927
1104	36-37	-0.04961161174594508
1104	38-39	-0.16390727223124202
1104	40-41	0.05729661505402106
1104	42-43	-0.009262224083400383
1104	44-45	0.02422172417686852
1104	46-47	-0.02089012896320952
1104	48-49	-0.3117949880095736
1104	50-51	-0.23596688165850566
1104	52-53	-0.08742320029209338
1104	54-55	-0.14093805712843022
1104	56-57	-0.2590708464187301
1104	58-59	-0.3278389260815615
1104	60-61	-0.21056549268359248
1104	62-63	-0.05906836126755621
1104	64-65	-0.12942639023254543
1104	66-67	-0.6659729808257211
1104	68-69	-0.573318256654872
1104	70-71	-0.09767121488967234
1104	72-73	-0.13852353274283047
1104	74-75	-0.24693276968667988
1104	76-77	-0.11307674685234304
1104	78-79	-0.1690270950852124
1104	80-81	-0.21572362321538918
1104	82-83	-0.23533750497941952
1104	84-85	-0.3395450703895122
1104	86-87	-0.31032064725506103
1104	88-89	-0.3013620241781325
1104	90-91	-0.3241627162736336
1104	92-93	-0.47887423736030854
1104	94-95	-0.3643797244749578
1104	96-97	-0.22945293938897038
1104	98-99	-0.24677462609488998
1104	100	-0.42737598772967544
1105	1	1.7907608304462954
1105	2	0.5016349903943329
1105	3	0.047655332710331066
1105	4	-0.19064186873201727
1105	5	-1.1857713570684005
1105	6	0.39564905869115563
1105	7	-0.10251444557025735
1105	8	-0.11499439915378673
1105	9	-0.15718296472431348
1105	10-11	-0.2156724343524985
1105	12-13	-0.24674854903162924
1105	14-15	-0.19319854016795546
1105	16-17	-0.0844646082921301
1105	18-19	-0.2907806019875707
1105	20-21	-0.3505738952449775
1105	22-23	-0.1593457746262743
1105	24-25	-0.13531636594570529
1105	26-27	-0.19266388660756206
1105	28-29	-0.22780060079576003
1105	30-31	-0.21633315410029752
1105	32-33	-0.3137450980481802
1105	34-35	-0.24406738837586772
1105	36-37	-0.05607825152954149
1105	38-39	-0.024613457018773488
1105	40-41	-0.2947998447809823
1105	42-43	-0.04153325588944057
1105	44-45	-0.1532784018375466
1105	46-47	-0.3351352186014651
1105	48-49	-0.5890010803865309
1105	50-51	-0.25055725079673863
1105	52-53	-0.2702205523091692
1105	54-55	-0.2732389123462653
1105	56-57	-0.33039080542204147
1105	58-59	-0.29049371378928157
1105	60-61	-0.3962603361736825
1105	62-63	-0.5024929290381053
1105	64-65	-0.35410463342356735
1105	66-67	-0.34816798393407566
1105	68-69	-0.39759745419506487
1105	70-71	-0.5562683384005993
1105	72-73	-0.36470386777315156
1105	74-75	-0.4700223911393664
1105	76-77	-0.5251850520424526
1105	78-79	-0.6743331420969838
1105	80-81	-0.43575865522305435
1105	82-83	-0.37944633102911496
1105	84-85	-0.4056644144424766
1105	86-87	-0.17472283486531381
1105	88-89	-0.3536118015526668
1105	90-91	-0.38588037677399
1105	92-93	-0.29660446569842946
1105	94-95	-0.18565389824043876
1105	96-97	-0.18469513909661828
1105	98-99	-0.49451476756707535
1105	100	-0.5252614658283719
1106	1	1.288625495841437
1106	2	-0.5766965462173257
1106	3	-0.08740147133643816
1106	4	-0.11359153288327306
1106	5	-1.1080866402593799
1106	6	-0.5931850071517815
1106	7	-0.08837231106490862
1106	8	0.0137343511571828
1106	9	0.03572855364371463
1106	10-11	0.06652260669568477
1106	12-13	0.009878451548843259
1106	14-15	0.006354076135224318
1106	16-17	-0.00713149609376984
1106	18-19	0.01356470027629797
1106	20-21	0.12077862154745844
1106	22-23	-0.11125347841597488
1106	24-25	-0.16259847889039492
1106	26-27	-0.3841391333112725
1106	28-29	-0.30669478771010006
1106	30-31	-0.17045911811057124
1106	32-33	-0.163555198388174
1106	34-35	-0.24793047818430836
1106	36-37	-0.29110644623888504
1106	38-39	0.0016714753968471996
1106	40-41	0.006446941851407928
1106	42-43	0.10231905358895688
1106	44-45	0.030281180217350823
1106	46-47	-0.32881338428684614
1106	48-49	-0.08640177696170781
1106	50-51	-0.2096902634843545
1106	52-53	-0.2249332150506902
1106	54-55	-0.3298016455745554
1106	56-57	-0.11151444751507711
1106	58-59	-0.1709361219491612
1106	60-61	-0.06614424031167232
1106	62-63	-0.10212971484123301
1106	64-65	-0.2973665125180176
1106	66-67	-0.22413448198532393
1106	68-69	-0.22179390497871765
1106	70-71	-0.4146077529457557
1106	72-73	-0.22452184604663472
1106	74-75	-0.5728024724064724
1106	76-77	-0.3611117457981621
1106	78-79	-0.26811206240547136
1106	80-81	-0.29409806976820363
1106	82-83	-0.38555255874285166
1106	84-85	-0.5209007524472895
1106	86-87	-0.5570252645858602
1106	88-89	-0.3401960954410441
1106	90-91	-0.6229630536128639
1106	92-93	-0.6224415997891555
1106	94-95	-0.2951998804943585
1106	96-97	-0.08963004248827389
1106	98-99	-0.7165680058123698
1106	100	-0.503112863124997
1107	1	1.294211853368319
1107	2	-0.6754784682603372
1107	3	-0.01058695520740649
1107	4	-0.26783800465746666
1107	5	-1.208750282732499
1107	6	-0.0878422652162989
1107	7	-0.289753359452007
1107	8	-0.30807260448797535
1107	9	-0.24135645979714582
1107	10-11	-0.07048512179893862
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2222	88-89	0.5127112793126116
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2223	1	1.346377181873775
2223	2	-0.7887465990327964
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2223	4	0.0484747708463118
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2223	7	0.2953182715303626
2223	8	0.10258053625509689
2223	9	0.33416765334307286
2223	10-11	0.19565480788199352
2223	12-13	0.24698578883656808
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2223	28-29	0.37853353763320285
2223	30-31	0.1688104033000002
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2223	34-35	0.17381771390149936
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2223	40-41	0.34742930960960194
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2223	46-47	0.25391148773720573
2223	48-49	0.17727458608371904
2223	50-51	0.290269515503212
2223	52-53	0.3495053158666792
2223	54-55	0.29839328414023925
2223	56-57	0.35356518196407194
2223	58-59	0.5787278904015309
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2223	62-63	0.4354777443283453
2223	64-65	0.5368262233233736
2223	66-67	0.5683872536123076
2223	68-69	0.5768089226803426
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2223	74-75	0.6464650176398408
2223	76-77	0.48404710688731
2223	78-79	0.7417493985517041
2223	80-81	0.568506139218556
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2223	84-85	0.5455029202593025
2223	86-87	0.555461937819274
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2223	90-91	0.5634584950992476
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2223	94-95	0.7893125920822257
2223	96-97	0.6100961296089054
2223	98-99	0.5669714432816448
2223	100	0.7026383353799233
2224	1	1.1206646515970853
2224	2	-0.7714675180896151
2224	3	-0.23125756980407708
2224	4	-0.39514124169331843
2224	5	-1.0259834640171768
2224	6	0.4773986118643663
2224	7	0.2067555233905054
2224	8	0.3622594017540237
2224	9	0.3114387680090829
2224	10-11	0.3061570703692169
2224	12-13	0.2517970009685371
2224	14-15	0.1888075063941983
2224	16-17	0.4063380012421476
2224	18-19	0.3147026127954007
2224	20-21	0.1759328902682853
2224	22-23	0.31181743976171816
2224	24-25	0.37575103166265933
2224	26-27	0.32000361172197955
2224	28-29	0.23621091630873536
2224	30-31	0.21317724552578454
2224	32-33	0.36340320894733935
2224	34-35	0.1912641027912798
2224	36-37	0.2814977419400435
2224	38-39	0.23899624512961282
2224	40-41	0.20812427859916482
2224	42-43	0.5628386521185504
2224	44-45	0.5298090807584259
2224	46-47	0.5110242928020838
2224	48-49	0.3242326454082942
2224	50-51	0.4620907451480818
2224	52-53	0.4370807455457353
2224	54-55	0.1903796769647812
2224	56-57	0.40055775107234126
2224	58-59	0.5508543864974129
2224	60-61	0.4944695302884625
2224	62-63	0.6111031132121312
2224	64-65	0.5636648368686252
2224	66-67	0.4080727791769547
2224	68-69	0.5646333388360745
2224	70-71	0.6661555364681462
2224	72-73	0.6005444461345988
2224	74-75	0.5285750006429666
2224	76-77	0.7646269680096154
2224	78-79	0.7903642500145054
2224	80-81	0.5312234782884673
2224	82-83	0.5245003097879746
2224	84-85	0.5249478287761278
2224	86-87	0.511300702949562
2224	88-89	0.4887093607565518
2224	90-91	0.47646158807891936
2224	92-93	0.5542582473877502
2224	94-95	0.8526279663521166
2224	96-97	0.6947216984559219
2224	98-99	0.628201313918332
2224	100	0.7453337107463867
2225	1	1.3893145030446945
2225	2	1.8926217542315715
2225	3	0.8823135302294869
2225	4	1.0567915968797497
2225	5	3.0579745352286665
2225	6	-0.2396929933716372
2225	7	0.16157653537000272
2225	8	0.17538588054438975
2225	9	-0.024050128890998224
2225	10-11	-0.028841209986644856
2225	12-13	-0.21195994494348014
2225	14-15	-0.11921017900623099
2225	16-17	-0.12496843105567024
2225	18-19	-0.0756147547816397
2225	20-21	-0.002239438792869919
2225	22-23	-0.04063232798273475
2225	24-25	-0.04099705911797713
2225	26-27	0.0334253306202541
2225	28-29	0.13426564657323325
2225	30-31	0.03271240292502853
2225	32-33	0.012925645123679885
2225	34-35	0.03884893511562382
2225	36-37	0.05743515518089026
2225	38-39	0.02653628383195894
2225	40-41	-0.0885692393783657
2225	42-43	0.08933857545972046
2225	44-45	0.032017269579490915
2225	46-47	0.24903816597936412
2225	48-49	0.09501381965589673
2225	50-51	0.084208890915896
2225	52-53	0.13718496012417347
2225	54-55	0.20045099039996472
2225	56-57	0.26797401683401745
2225	58-59	0.43253292492421025
2225	60-61	0.30012363733735015
2225	62-63	0.26176735165839915
2225	64-65	0.06492287050735257
2225	66-67	0.2632420708907617
2225	68-69	0.13972191086844532
2225	70-71	0.136268121362626
2225	72-73	0.3044550826515504
2225	74-75	0.15318145075877965
2225	76-77	0.4710878575007271
2225	78-79	0.28554489552332996
2225	80-81	0.09043476246898052
2225	82-83	0.09378870861281996
2225	84-85	0.1373474989359451
2225	86-87	0.20097765847500426
2225	88-89	0.21949907106903055
2225	90-91	0.002872628608656669
2225	92-93	-0.02735129808750969
2225	94-95	0.30369248822889006
2225	96-97	0.4825497257547582
2225	98-99	0.5030572160270737
2225	100	0.44201293288711696
2226	1	1.5523456466583614
2226	2	1.046805757930139
2226	3	0.8420916162211647
2226	4	1.082177214498378
2226	5	3.166672471596506
2226	6	-0.260528953527988
2226	7	0.019641932755781966
2226	8	0.12769748479773568
2226	9	0.1430557425838046
2226	10-11	0.01738351997595089
2226	12-13	0.04820270793761949
2226	14-15	0.06869592783191081
2226	16-17	0.036948939599568575
2226	18-19	-0.014074595911388599
2226	20-21	0.08595666930615664
2226	22-23	0.1557720766740971
2226	24-25	0.1859394348088088
2226	26-27	-0.05961043620192186
2226	28-29	0.06997257718307992
2226	30-31	0.16902140136994603
2226	32-33	0.05646155904793915
2226	34-35	0.05638665629411577
2226	36-37	-0.03266907715635625
2226	38-39	0.10383527933484515
2226	40-41	0.030083305487771383
2226	42-43	0.13536471760235713
2226	44-45	0.21720619069075298
2226	46-47	0.15207057046807648
2226	48-49	0.2428671798878952
2226	50-51	0.07514635151354554
2226	52-53	0.38704416241056094
2226	54-55	0.2211598123223837
2226	56-57	0.33271889978278324
2226	58-59	0.1581024201538952
2226	60-61	0.3366270206853912
2226	62-63	0.3007546588495913
2226	64-65	0.30651283394197293
2226	66-67	0.2716405494348706
2226	68-69	0.2009878294159222
2226	70-71	0.3952665662834036
2226	72-73	0.2560227706270055
2226	74-75	0.19556786693161143
2226	76-77	0.4034965491327185
2226	78-79	0.5376022233088094
2226	80-81	0.3329840416687446
2226	82-83	0.26974907510716406
2226	84-85	0.21448047922427094
2226	86-87	0.36844813298430523
2226	88-89	0.184451998400597
2226	90-91	0.3626423087666879
2226	92-93	0.3651097198589426
2226	94-95	0.5762789632425509
2226	96-97	0.43247092106430074
2226	98-99	0.6133942899563749
2226	100	0.6004187665356682
2227	1	1.0818935546697457
2227	2	1.8875758191032244
2227	3	0.531368334465137
2227	4	-0.02394336517383877
2227	5	3.130647413531147
2227	6	0.29176258365020047
2227	7	0.3375097072986222
2227	8	0.21404149343393897
2227	9	0.2031852855848868
2227	10-11	0.23564221342356007
2227	12-13	0.10844674601541016
2227	14-15	0.38921017429074567
2227	16-17	0.18854941720760365
2227	18-19	0.2433790335825492
2227	20-21	0.11730133219993633
2227	22-23	0.13973295207074443
2227	24-25	0.21262578372438412
2227	26-27	0.3021064992555651
2227	28-29	0.28407509934605457
2227	30-31	0.2846794209322496
2227	32-33	0.2141286400777389
2227	34-35	0.25379252498461824
2227	36-37	0.23825408029073714
2227	38-39	0.2124003288948586
2227	40-41	0.37135878064233907
2227	42-43	0.32915581315668163
2227	44-45	0.34319984439220974
2227	46-47	0.2831936036062288
2227	48-49	0.253471059635352
2227	50-51	0.3242745532865925
2227	52-53	0.32903932090445664
2227	54-55	0.23038320857349248
2227	56-57	0.41307399808498246
2227	58-59	0.35454653020001814
2227	60-61	0.24870625127847745
2227	62-63	0.3791175428170064
2227	64-65	0.2858816389405021
2227	66-67	0.34515047779365915
2227	68-69	0.5182446909423035
2227	70-71	0.5229334837488082
2227	72-73	0.29219912575802454
2227	74-75	0.275873078093241
2227	76-77	0.38927380713919746
2227	78-79	0.3192053179471728
2227	80-81	0.3399167185636429
2227	82-83	0.2625424278501427
2227	84-85	0.5335381847429304
2227	86-87	0.5120110562071858
2227	88-89	0.3726246569762779
2227	90-91	0.3055869585753186
2227	92-93	0.3223629829686274
2227	94-95	0.48484155933662976
2227	96-97	0.4211792530346017
2227	98-99	0.47314272791640377
2227	100	0.36384740338999677
2228	1	1.108292244589693
2228	2	1.8568748237243948
2228	3	-0.027683011187050965
2228	4	-0.06668600879232756
2228	5	3.0650152229926917
2228	6	0.31645957956741455
2228	7	0.20402447133679402
2228	8	0.13135070180973685
2228	9	0.05505138053364078
2228	10-11	0.10340761038935398
2228	12-13	0.09375721704174822
2228	14-15	0.017795428839818328
2228	16-17	0.06790379898954058
2228	18-19	0.03833692386654519
2228	20-21	0.11713789266295294
2228	22-23	0.24537337867998588
2228	24-25	0.06491650099962243
2228	26-27	-0.07332693598713291
2228	28-29	0.10739615869062646
2228	30-31	0.23090220504464298
2228	32-33	0.09616695543272868
2228	34-35	0.1915565118838174
2228	36-37	0.0838389695043702
2228	38-39	0.03339477835999105
2228	40-41	0.08063269248754068
2228	42-43	0.3688211466694753
2228	44-45	0.36609419835738066
2228	46-47	0.24514664197926805
2228	48-49	0.16890151451001856
2228	50-51	0.24271021258886094
2228	52-53	0.31808246253282846
2228	54-55	0.1333297670495952
2228	56-57	0.4665627912602588
2228	58-59	0.3008942974877584
2228	60-61	0.1796642633409533
2228	62-63	0.29776070623731243
2228	64-65	0.285939323482971
2228	66-67	0.13210864368158326
2228	68-69	0.3808240873056121
2228	70-71	0.32486158958071343
2228	72-73	0.2889735984251658
2228	74-75	0.32354694891832025
2228	76-77	0.501106509147256
2228	78-79	0.3686818307242987
2228	80-81	0.3553585501119585
2228	82-83	0.2485683474378888
2228	84-85	0.26544046071860095
2228	86-87	0.2021961915415318
2228	88-89	0.29039694404019656
2228	90-91	0.29272971499913325
2228	92-93	0.40947705735604956
2228	94-95	0.5482032221897128
2228	96-97	0.41998950934624446
2228	98-99	0.5212740692102074
2228	100	0.4444919961050786
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	12.0
13	25.0
14	93.0
15	297.0
16	1159.0
17	3055.0
18	4896.0
19	6173.0
20	7119.0
21	8430.0
22	9742.0
23	10817.0
24	12261.0
25	14121.0
26	15763.0
27	18158.0
28	20148.0
29	23085.0
30	26447.0
31	30131.0
32	35591.0
33	42163.0
34	51919.0
35	66982.0
36	94190.0
37	155350.0
38	379863.0
39	711266.0
40	814.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.449694568757643	19.33044263519772	20.840678787660025	33.37918400838461
2	19.58721059140043	53.56833460194246	9.55536964526946	17.289085161387653
3	40.56940579519676	15.038712737204799	23.60848423205929	20.78339723553915
4	17.609809893318555	16.41025787539927	23.955841766329346	42.02409046495283
5	17.02137628780563	34.81523596199009	26.89395281331604	21.26943493688824
6	16.678304296399574	27.521699132034716	31.557137714491418	24.24285885707429
7	20.037655636631676	26.951550509408197	36.94343654825236	16.067357305707773
8	20.046798128074876	27.685863994011665	35.36909952173342	16.89823835618004
9	20.245018770677746	28.184529761666678	28.366751044243944	23.203700423411636
10-11	18.960213020050627	30.6275177564326	29.07552269337798	21.336746530138797
12-13	23.885958847360392	26.5936505396927	27.69257801116527	21.827812601781645
14-15	21.770386327404047	27.445416469055523	28.71250864251144	22.07168856102899
16-17	20.75114138291611	30.12568068705823	28.17647294108236	20.9467049889433
18-19	21.25800682258424	29.670784597187538	30.02819887204512	19.0430097081831
20-21	22.60815281674447	29.115263960870134	30.054797808087674	18.221785414297713
22-23	22.144914203431863	30.409983600655977	28.702280480209364	18.7428217157028
24-25	22.143314267429304	28.471975406698018	26.49716868468118	22.887541641191493
26-27	19.87240510379585	26.379544818207272	30.399326884067495	23.348723193929384
28-29	19.14268898583767	28.466399819435278	30.960592892264	21.43031830246305
30-31	19.930018278338782	30.290038976835916	28.39434351561859	21.38559922920671
32-33	20.176792928282868	30.410926420086053	29.20228905129509	20.209991600335986
34-35	21.2843200557692	29.414594844777636	30.043798248070075	19.25728685138309
36-37	21.777871742273167	28.265555092082028	28.989269000668543	20.96730416497626
38-39	21.490598015149228	29.148038691333305	29.10392581723099	20.257437476286473
40-41	20.731148793413638	30.234100296535992	29.823338374169055	19.211412535881312
42-43	20.314559295570028	30.12575229241158	29.77262305596784	19.78706535605055
44-45	20.49549446593565	30.129451964778553	29.006268320695742	20.368785248590058
46-47	19.697189172911536	28.302438263430506	29.0959074282165	22.90446513544146
48-49	20.652191614213958	27.93799214145046	30.71091217286638	20.6989040714692
50-51	21.614421137440218	30.889792979709384	28.186129697669237	19.309656185181165
52-53	20.4013313905317	30.491500242850204	28.41415959544013	20.69300877117797
54-55	22.358067646544463	28.307801950230377	28.543722445937743	20.790407957287417
56-57	21.72724760861695	28.70531148912509	29.567486619770023	19.999954282487938
58-59	21.191174558635065	28.205723135566217	29.087695787156164	21.515406518642553
60-61	19.398812103640513	30.622344211098024	29.793119526464253	20.185724158797214
62-63	20.473721581776903	31.451867069935297	27.427762387723643	20.646648960564157
64-65	19.607975344965066	30.24284478007575	29.12934550847581	21.01983436648337
66-67	20.05159793608256	30.581148182644124	29.457021719131234	19.910232162142087
68-69	20.709057351991635	30.183792648294066	29.52036204266115	19.586787957053147
70-71	19.54913232042147	31.206951721931127	29.382396132726118	19.861519824921288
72-73	19.88586170838881	30.930477066631624	28.73819332940968	20.44546789556989
74-75	19.83423520202049	29.22945939305285	30.842309164776267	20.093996240150393
76-77	19.308599084608044	30.6706588879302	28.78144874205032	21.23929328541144
78-79	19.442736576251235	29.628272011976662	28.66505339786409	22.263938013908017
80-81	20.25405313053729	29.72440483707195	28.769612820537727	21.25192921185303
82-83	19.947602095916164	28.743393121418	30.176135811710385	21.13286897095545
84-85	20.383813218899817	29.169318941528054	28.450033427234338	21.996834412337794
86-87	21.57682264138006	28.883958927357188	29.227716605621488	20.31150182564126
88-89	21.83915500522836	28.74356454313256	28.855988617598154	20.561291834040922
90-91	22.085602290194107	29.84277771746273	27.95902449616301	20.112595496180152
92-93	21.621278006022614	30.280817338735023	28.79076265520808	19.307142000034283
94-95	21.5476523796191	30.62868913814876	28.325781254464104	19.497877227768033
96-97	21.477483757792545	29.99374310741856	28.59962744347369	19.929145691315203
98-99	22.265252247052974	30.46243864531133	28.01173667339021	19.26057243424549
100	22.45110195592176	32.15322815658802	26.55625203563286	18.839417851857355
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	11.0
2	13.5
3	11.0
4	17.0
5	20.5
6	21.0
7	35.5
8	41.5
9	35.5
10	43.0
11	56.0
12	67.5
13	77.0
14	98.5
15	129.5
16	167.5
17	233.0
18	354.5
19	574.0
20	906.5
21	1372.5
22	2048.0
23	3185.0
24	4922.0
25	7220.0
26	10231.5
27	13764.5
28	17476.0
29	21931.5
30	27337.5
31	33289.0
32	39884.5
33	46834.0
34	54779.0
35	63870.5
36	72325.0
37	78545.5
38	82346.0
39	85120.0
40	86573.5
41	85791.0
42	84002.5
43	82493.5
44	81207.5
45	78373.5
46	71705.0
47	63856.0
48	58950.0
49	56889.5
50	55409.0
51	51904.5
52	45705.5
53	38180.0
54	30953.5
55	24931.5
56	19797.5
57	14728.0
58	10272.5
59	7355.5
60	5696.0
61	4757.5
62	3958.0
63	3122.5
64	2390.5
65	1860.0
66	1634.0
67	1465.0
68	1251.0
69	1113.5
70	1118.5
71	994.0
72	686.5
73	421.5
74	235.5
75	143.0
76	119.5
77	88.5
78	62.5
79	51.0
80	37.5
81	27.0
82	26.0
83	19.5
84	15.0
85	14.5
86	11.5
87	10.5
88	10.0
89	5.5
90	5.0
91	7.5
92	8.5
93	11.5
94	9.5
95	8.0
96	11.0
97	12.5
98	26.0
99	59.0
100	64.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.351374516447914
2	0.006971149725439553
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	2.8570285759998174E-4
30-31	0.010542435445439325
32-33	0.0
34-35	0.0
36-37	0.0
38-39	7.999680012799489E-4
40-41	0.001685646859839892
42-43	1.4285142879999087E-4
44-45	0.0
46-47	0.0019142091459198776
48-49	8.571085727999452E-5
50-51	0.0
52-53	0.001142811430399927
54-55	0.0187421074585588
56-57	0.011056700589119292
58-59	0.013856588593599112
60-61	8.571085727999452E-5
62-63	0.01157096573279926
64-65	8.571085727999452E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.7142171455998903E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1750070.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.88853378957225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.3078543296129	69.66477150166291
2	7.539838696026228	11.896136394787789
3	2.006751592123555	4.74929072347551
4	0.7819773120210729	2.467561744082132
5	0.4298452642819428	1.6954931327796827
6	0.23836020911355482	1.1282332446446506
7	0.15036843891230764	0.8303641971813168
8	0.10850514816291748	0.6847849637754286
9	0.07291231414388882	0.5176751002214472
>10	0.34174929402481896	4.566868411325686
>50	0.016101112501011674	0.869970232586679
>100	0.005515720521366099	0.8083820697888161
>500	2.2056855436344743E-4	0.12046828368793276
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.7140571519996346E-5	0.0
3	0.0	0.0	0.0	5.7140571519996346E-5	0.0
4	0.0	0.0	0.0	5.7140571519996346E-5	0.0
5	0.0	0.0	0.0	5.7140571519996346E-5	0.0
6	0.0	0.0	0.0	5.7140571519996346E-5	0.0
7	0.0	0.0	0.0	5.7140571519996346E-5	0.0
8	0.0	0.0	0.0	5.7140571519996346E-5	0.0
9	0.0	0.0	0.0	5.7140571519996346E-5	0.0
10-11	0.0	0.0	0.0	5.7140571519996346E-5	0.0
12-13	0.0	2.8570285759998173E-5	0.0	5.7140571519996346E-5	0.0
14-15	0.0	5.7140571519996346E-5	0.0	8.571085727999452E-5	0.0
16-17	0.0	5.7140571519996346E-5	0.0	1.1428114303999269E-4	0.0
18-19	0.0	5.7140571519996346E-5	0.0	1.1428114303999269E-4	0.0
20-21	0.0	5.7140571519996346E-5	0.0	1.1428114303999269E-4	0.0
22-23	0.0	5.7140571519996346E-5	0.0	1.1428114303999269E-4	0.0
24-25	0.0	5.7140571519996346E-5	0.0	1.1428114303999269E-4	0.0
26-27	0.0	5.7140571519996346E-5	0.0	1.1428114303999269E-4	0.0
28-29	0.0	5.7140571519996346E-5	0.0	1.1428114303999269E-4	0.0
30-31	0.0	5.7140571519996346E-5	0.0	1.1428114303999269E-4	0.0
32-33	0.0	5.7140571519996346E-5	0.0	1.1428114303999269E-4	0.0
34-35	0.0	5.7140571519996346E-5	0.0	1.1428114303999269E-4	0.0
36-37	5.7140571519996346E-5	5.7140571519996346E-5	0.0	1.1428114303999269E-4	0.0
38-39	5.7140571519996346E-5	5.7140571519996346E-5	0.0	1.1428114303999269E-4	0.0
40-41	5.7140571519996346E-5	5.7140571519996346E-5	0.0	1.1428114303999269E-4	0.0
42-43	5.7140571519996346E-5	5.7140571519996346E-5	0.0	1.1428114303999269E-4	0.0
44-45	5.7140571519996346E-5	5.7140571519996346E-5	0.0	1.1428114303999269E-4	0.0
46-47	5.7140571519996346E-5	5.7140571519996346E-5	0.0	1.1428114303999269E-4	0.0
48-49	5.7140571519996346E-5	5.7140571519996346E-5	0.0	1.1428114303999269E-4	0.0
50-51	1.1428114303999269E-4	5.7140571519996346E-5	0.0	1.1428114303999269E-4	0.0
52-53	1.4285142879999087E-4	5.7140571519996346E-5	0.0	1.1428114303999269E-4	0.0
54-55	1.7142171455998903E-4	5.7140571519996346E-5	0.0	1.1428114303999269E-4	0.0
56-57	2.8570285759998174E-4	5.7140571519996346E-5	1.999920003199872E-4	1.1428114303999269E-4	0.0
58-59	2.8570285759998174E-4	5.7140571519996346E-5	3.999840006399744E-4	1.1428114303999269E-4	0.0
60-61	4.5712457215997077E-4	5.7140571519996346E-5	3.999840006399744E-4	1.1428114303999269E-4	0.0
62-63	5.714057151999635E-4	5.7140571519996346E-5	3.999840006399744E-4	1.1428114303999269E-4	0.0
64-65	5.714057151999635E-4	5.7140571519996346E-5	3.999840006399744E-4	1.1428114303999269E-4	0.0
66-67	7.142571439999543E-4	5.7140571519996346E-5	3.999840006399744E-4	1.1428114303999269E-4	0.0
68-69	8.285382870399469E-4	5.7140571519996346E-5	3.999840006399744E-4	1.7142171455998903E-4	0.0
70-71	9.142491443199414E-4	5.7140571519996346E-5	3.999840006399744E-4	2.2856228607998538E-4	0.0
72-73	0.0011713817161599252	5.7140571519996346E-5	3.999840006399744E-4	2.2856228607998538E-4	0.0
74-75	0.001314233144959916	5.7140571519996346E-5	3.999840006399744E-4	2.2856228607998538E-4	0.0
76-77	0.0013999440022399103	5.7140571519996346E-5	3.999840006399744E-4	2.2856228607998538E-4	0.0
78-79	0.0015427954310399013	5.7140571519996346E-5	3.999840006399744E-4	2.2856228607998538E-4	0.0
80-81	0.0017713577171198868	5.7140571519996346E-5	3.999840006399744E-4	2.2856228607998538E-4	0.0
82-83	0.001828498288639883	5.7140571519996346E-5	3.999840006399744E-4	2.2856228607998538E-4	0.0
84-85	0.0018570685743998813	5.7140571519996346E-5	3.999840006399744E-4	2.2856228607998538E-4	0.0
86-87	0.0019427794316798756	1.1428114303999269E-4	3.999840006399744E-4	2.2856228607998538E-4	0.0
88	0.0019427794316798756	1.1428114303999269E-4	3.999840006399744E-4	2.2856228607998538E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	7125	0.0	80.90037	8
CACCGGC	6730	0.0	80.347885	9
CGCTGCA	40	4.2868123E-8	79.37854	1
CTTCACC	8085	0.0	75.88092	6
ATGCAGC	25	5.5958873E-5	75.10295	2
CCTTCAC	8830	0.0	72.88091	5
GTGTGGC	3000	0.0	68.21851	7
TTCACCG	8920	0.0	67.0938	7
GGGCCCG	400	0.0	65.71509	8
CGTAGTG	1295	0.0	65.60634	4
TGTGGCA	3325	0.0	64.51513	8
ACCTTCA	3505	0.0	64.41605	4
GAGCTGC	25	0.0032737008	63.50283	1
CGGGGCA	725	0.0	63.449047	94
CGGGCCC	415	0.0	62.208767	7
TAGCAGC	35	1.6348061E-4	60.47888	1
GCCTTCA	2335	0.0	60.106483	4
GCTGCCA	95	0.0	59.2918	2
CAGCGTA	680	0.0	59.144794	1
GCGTAGT	890	0.0	58.542328	3
>>END_MODULE