FastQCFastQC Report
Mon 6 Mar 2017
1NF_18A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_18A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1750070
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG71250.080.900378
CACCGGC67300.080.3478859
CGCTGCA404.2868123E-879.378541
CTTCACC80850.075.880926
ATGCAGC255.5958873E-575.102952
CCTTCAC88300.072.880915
GTGTGGC30000.068.218517
TTCACCG89200.067.09387
GGGCCCG4000.065.715098
CGTAGTG12950.065.606344
TGTGGCA33250.064.515138
ACCTTCA35050.064.416054
GAGCTGC250.003273700863.502831
CGGGGCA7250.063.44904794
CGGGCCC4150.062.2087677
TAGCAGC351.6348061E-460.478881
GCCTTCA23350.060.1064834
GCTGCCA950.059.29182
CAGCGTA6800.059.1447941
GCGTAGT8900.058.5423283
Produced by FastQC (version 0.11.5)