FastQCFastQC Report
Mon 6 Mar 2017
1NF_19A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_19A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2036640
Sequences flagged as poor quality0
Sequence length95
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA140620.6904509388011627No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA57710.2833588655825281No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG54570.26794131510723546No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT45350.22267067326577106No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG44710.21952824259564774No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA43400.21309607981773904No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG42570.20902074004242283No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG39880.19581271113206064No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT36690.18014965826066462No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT35630.17494500746327285No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC35040.17204807918925288No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA33210.16306269149186897No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA30870.1515731793542305No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA29170.14322609788671536No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC27530.13517361929452432No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA26860.13188388718673894No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA25780.1265810354309058No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTTAAAAAAAAGCCATGAGTGCTC22530.11062337968418572No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC21820.10713724565951763No Hit
CAGCACTTTGAACTTTGTTCACCATTTCTTCGTAGTCGTTGATACGTTGT21120.10370021211407023No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTT158.5776724E-488.999833
GCTGCAG202.4111883E-588.999833
CAGCTGC3600.088.999831
GCAGCTT158.5776724E-488.999833
GCAGCTA158.5776724E-488.999833
CAGCAGC3650.088.9998251
GCTGCCC850.088.9998253
AGCTGCC1400.088.9998252
GCAGCAT355.5479177E-1088.9998253
AGCAGCG750.088.9998252
AGCAGCA1650.088.9998252
AGCTGCT1050.084.7617342
AGCTGCA900.084.05542
CAGCCCT135450.083.480461
GCAGCAA800.083.437343
AGCAGCC750.083.0665052
CAGCGTA45300.081.3375851
AGCCCTT118100.080.898672
GCCCTTC106800.079.999853
CAGCTCT49900.079.903661
Produced by FastQC (version 0.11.5)