FastQCFastQC Report
Mon 6 Mar 2017
1NF_19A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_19A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2036640
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG85850.080.477428
CACCGGC83050.079.799759
CTTCACC96250.074.707566
GTGTGGC38450.074.3408367
CCTTCAC107100.070.951875
CGGGGCA9250.070.5309294
CGTAGTG17050.066.894194
TGTGGCA43500.066.5736548
AACGGGC6350.065.045235
TTCACCG111000.064.399647
GCAGCCT501.9821528E-763.6347161
ATGCTGC301.380348E-462.584472
TGTGTGG44100.062.3685346
CGGGCCC6750.061.8860477
ACCTTCA42600.061.2592544
GGCCCGA6850.060.9825979
GGGCCCG6900.060.5406958
ATGTGTG43000.059.925325
GCCTTCA32100.059.0721554
GGCTGCC457.898501E-658.9210321
Produced by FastQC (version 0.11.5)