##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1NF_19A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2036640 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.817348672323043 12.0 12.0 12.0 2.0 32.0 2 14.53343742634928 12.0 12.0 12.0 12.0 22.0 3 13.745782268834944 12.0 12.0 12.0 12.0 22.0 4 13.71304943436248 12.0 12.0 12.0 12.0 22.0 5 22.3983178175819 22.0 22.0 22.0 22.0 27.0 6 26.707144119726607 27.0 22.0 32.0 22.0 32.0 7 34.824784448896224 37.0 37.0 37.0 27.0 41.0 8 37.44782730379448 41.0 37.0 41.0 27.0 41.0 9 38.43212791656847 41.0 37.0 41.0 32.0 41.0 10-11 38.807191992693845 41.0 41.0 41.0 34.5 41.0 12-13 38.98782725469401 41.0 41.0 41.0 37.0 41.0 14-15 38.932174562023725 41.0 41.0 41.0 37.0 41.0 16-17 38.82814316717731 41.0 41.0 41.0 37.0 41.0 18-19 38.75539172362322 41.0 41.0 41.0 34.5 41.0 20-21 38.78362449917511 41.0 41.0 41.0 37.0 41.0 22-23 38.77347125657946 41.0 41.0 41.0 34.5 41.0 24-25 38.788350174797706 41.0 41.0 41.0 34.5 41.0 26-27 38.752345284389975 41.0 41.0 41.0 32.0 41.0 28-29 38.70112464647655 41.0 41.0 41.0 32.0 41.0 30-31 38.57458240042423 41.0 41.0 41.0 32.0 41.0 32-33 38.62203506756226 41.0 41.0 41.0 32.0 41.0 34-35 38.588623909969364 41.0 41.0 41.0 32.0 41.0 36-37 38.51130366682379 41.0 41.0 41.0 32.0 41.0 38-39 38.412573405216435 41.0 41.0 41.0 32.0 41.0 40-41 38.45377950938801 41.0 41.0 41.0 32.0 41.0 42-43 38.188359258386356 41.0 41.0 41.0 32.0 41.0 44-45 38.31191644080447 41.0 41.0 41.0 32.0 41.0 46-47 38.30870551496582 41.0 41.0 41.0 32.0 41.0 48-49 38.248641880744756 41.0 41.0 41.0 32.0 41.0 50-51 38.16111880351952 41.0 41.0 41.0 32.0 41.0 52-53 38.12258990297745 41.0 37.0 41.0 32.0 41.0 54-55 37.99280727079896 41.0 37.0 41.0 32.0 41.0 56-57 37.76043802537513 41.0 37.0 41.0 27.0 41.0 58-59 37.75625908358866 41.0 37.0 41.0 27.0 41.0 60-61 37.7462447953492 41.0 37.0 41.0 27.0 41.0 62-63 37.75079002671066 41.0 37.0 41.0 27.0 41.0 64-65 37.73070105664231 41.0 37.0 41.0 27.0 41.0 66-67 37.59911545486684 41.0 37.0 41.0 27.0 41.0 68-69 37.631026101814754 41.0 37.0 41.0 27.0 41.0 70-71 37.62352133906827 41.0 37.0 41.0 27.0 41.0 72-73 37.45185108806662 41.0 37.0 41.0 27.0 41.0 74-75 37.36792658496347 41.0 37.0 41.0 27.0 41.0 76-77 36.32405800730615 39.0 34.5 41.0 24.5 41.0 78-79 36.83217750805248 41.0 37.0 41.0 27.0 41.0 80-81 37.50903424267421 41.0 37.0 41.0 27.0 41.0 82-83 37.7067353582371 41.0 37.0 41.0 27.0 41.0 84-85 37.62081492065363 41.0 37.0 41.0 27.0 41.0 86-87 37.47533805679943 41.0 37.0 41.0 27.0 41.0 88-89 37.37726721462801 41.0 37.0 41.0 27.0 41.0 90-91 37.20951690038495 41.0 37.0 41.0 27.0 41.0 92-93 37.10908187013905 41.0 37.0 41.0 27.0 41.0 94-95 36.939328256343785 41.0 37.0 41.0 27.0 41.0 96-97 36.87756304501532 41.0 37.0 41.0 27.0 41.0 98-99 36.761897537119964 41.0 37.0 41.0 27.0 41.0 100 36.63437917354074 41.0 37.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3755211784472259 1101 2 -0.521521272109176 1101 3 -0.01870370148129119 1101 4 -7.571620326469741E-4 1101 5 -1.0808363161382921 1101 6 -0.786627958192863 1101 7 -0.26545058166539093 1101 8 -0.18469422030479166 1101 9 -0.018856237575633372 1101 10-11 -0.15948818374032925 1101 12-13 -0.007184284256545936 1101 14-15 0.024392636340444085 1101 16-17 0.032825466105457224 1101 18-19 0.14010834394755278 1101 20-21 -0.04532552323011885 1101 22-23 -0.04853326725679352 1101 24-25 -0.0249044086273571 1101 26-27 0.12890681587192176 1101 28-29 0.06942333458358974 1101 30-31 -0.057603670905052695 1101 32-33 -0.1431104360004909 1101 34-35 -0.06329286354068131 1101 36-37 -0.0242555858331599 1101 38-39 0.07195193952976098 1101 40-41 0.15915341942186245 1101 42-43 0.2071354803231884 1101 44-45 0.07517614723803234 1101 46-47 -6.810860106796213E-4 1101 48-49 0.1818105522511857 1101 50-51 0.06673510544364092 1101 52-53 0.015387418559598132 1101 54-55 -0.022857228559288956 1101 56-57 0.06238186911267718 1101 58-59 0.1281282564538273 1101 60-61 0.08238734365161093 1101 62-63 -0.17530657682298312 1101 64-65 -0.17262082394218226 1101 66-67 -0.18170091462486226 1101 68-69 -0.19769052301985823 1101 70-71 -0.16977993887391563 1101 72-73 -0.06307943806509542 1101 74-75 0.1604824060918375 1101 76-77 -0.3967143408166862 1101 78-79 -0.17442846823150404 1101 80-81 -0.15386970473788608 1101 82-83 -0.1347974810820034 1101 84-85 -0.3318835890282017 1101 86-87 -7.486578440136782E-4 1101 88-89 -0.19530134684239187 1101 90-91 -0.24658957296369977 1101 92-93 -0.10891121136688753 1101 94-95 -0.14913239580744886 1101 96-97 -0.33219016922953415 1101 98-99 -0.4695544339357056 1101 100 -0.9062115648579194 1102 1 1.467648481629805 1102 2 0.6928840880248259 1102 3 0.02317234541988178 1102 4 0.07182798592938511 1102 5 -0.729077522168442 1102 6 -0.1339199738265897 1102 7 -0.062212167371697547 1102 8 -0.33712303102506525 1102 9 -0.42030794053487597 1102 10-11 -0.2281649006582498 1102 12-13 -0.2765868526540842 1102 14-15 -0.2277011660046142 1102 16-17 -0.3074872083780704 1102 18-19 -0.08518478838075083 1102 20-21 -0.05956337917651666 1102 22-23 -0.14540652242150998 1102 24-25 -0.12010262191601839 1102 26-27 -0.25216521092875155 1102 28-29 -0.28093959115621203 1102 30-31 -0.1821151170245372 1102 32-33 -0.3801288614611238 1102 34-35 -0.16463289704152118 1102 36-37 -0.29114559141663676 1102 38-39 -0.21587608950430592 1102 40-41 -0.2495567983335789 1102 42-43 -0.1641654688672176 1102 44-45 -0.11717449486135223 1102 46-47 -0.4906308347544055 1102 48-49 -0.3701157459062685 1102 50-51 -0.31712865781710775 1102 52-53 -0.45837025871567505 1102 54-55 -0.47735192426002016 1102 56-57 -0.13359803038481033 1102 58-59 -0.09074388201630512 1102 60-61 -0.26155458823001965 1102 62-63 -0.34783589005581206 1102 64-65 -0.3225370718483731 1102 66-67 -0.19426372869521202 1102 68-69 -0.5017106235223707 1102 70-71 -0.5162344335696218 1102 72-73 -0.19959535096292313 1102 74-75 -0.46654160284171553 1102 76-77 -0.4961001587954641 1102 78-79 -0.25455130463574704 1102 80-81 -0.14298193254358438 1102 82-83 -0.34529441017190265 1102 84-85 -0.2830282009768297 1102 86-87 -0.31621487783284863 1102 88-89 -0.1414208331628899 1102 90-91 -0.2731110693344476 1102 92-93 -0.33113343358911607 1102 94-95 -0.27559805744899535 1102 96-97 -0.17389871194310302 1102 98-99 -0.22527749367886685 1102 100 -0.5639446748523369 1103 1 1.4518167803139033 1103 2 0.5935218849662736 1103 3 0.35902665236079123 1103 4 1.0542102849615613 1103 5 2.9111559614593006 1103 6 -0.471348069629407 1103 7 -0.7207533605268637 1103 8 -0.3415661944409578 1103 9 -0.46669993656497866 1103 10-11 -0.21429129676909753 1103 12-13 -0.46457342514574407 1103 14-15 -0.3432227678337654 1103 16-17 -0.4696605703598564 1103 18-19 -0.36191154046226615 1103 20-21 -0.11655766565979064 1103 22-23 -0.12876204731001906 1103 24-25 -0.3540981758411732 1103 26-27 -0.24980565985808312 1103 28-29 -0.15261773208617768 1103 30-31 -0.2343618340653819 1103 32-33 -0.3579251753338255 1103 34-35 -0.36456392853476416 1103 36-37 -0.3575733915477173 1103 38-39 -0.026941200044774405 1103 40-41 -0.205466610041789 1103 42-43 -0.16506833520093522 1103 44-45 -0.5067985194519267 1103 46-47 -0.426554336646511 1103 48-49 -0.4356343716084936 1103 50-51 -0.4604552107569049 1103 52-53 -0.16526025135946298 1103 54-55 -0.17299700947752683 1103 56-57 -0.3007192033928945 1103 58-59 -0.4870635452222487 1103 60-61 -0.10443789235202416 1103 62-63 -0.24525300006833817 1103 64-65 -0.28809487243881904 1103 66-67 -0.34287581836979797 1103 68-69 -0.47143451658106983 1103 70-71 -0.5040533401276406 1103 72-73 -0.5178324421740967 1103 74-75 -0.4398252025060643 1103 76-77 -0.6754460966733404 1103 78-79 -0.5325304562195967 1103 80-81 -0.29852322209938364 1103 82-83 -0.5100110667132611 1103 84-85 -0.4871505573188628 1103 86-87 -0.5950114938471316 1103 88-89 -0.32645327618137543 1103 90-91 -0.6899535539346076 1103 92-93 -0.6065295010902645 1103 94-95 -0.5035849081438215 1103 96-97 -0.31873597279351173 1103 98-99 -0.3389737915710569 1103 100 -0.11126865150138343 1104 1 1.4077856528627617 1104 2 -0.4852588533258526 1104 3 -0.0015803105388325633 1104 4 -0.07774179462647979 1104 5 -1.0336878709878974 1104 6 -0.644715693312147 1104 7 -0.18199654818550215 1104 8 -0.09971020732091773 1104 9 0.16693723614125844 1104 10-11 0.0061663792648332105 1104 12-13 -0.13636185044006055 1104 14-15 -0.014113805285916214 1104 16-17 -0.01923478461437611 1104 18-19 -0.09635522587553425 1104 20-21 0.0963258198347745 1104 22-23 -0.0434752883343279 1104 24-25 -0.1104515302502449 1104 26-27 -0.228836197724263 1104 28-29 -0.23956142244010437 1104 30-31 0.05478792512703734 1104 32-33 -0.08121467077425137 1104 34-35 -0.14047223522698005 1104 36-37 -0.2756161407395865 1104 38-39 -0.215620246458613 1104 40-41 -0.026736233504500717 1104 42-43 -0.08289435427714409 1104 44-45 -0.15219626532697106 1104 46-47 -0.3876521043263139 1104 48-49 -0.35859455584885325 1104 50-51 -0.2501125400226556 1104 52-53 -0.2569048113347492 1104 54-55 -0.21362138983690215 1104 56-57 -0.10760805905129445 1104 58-59 -0.25467467869440696 1104 60-61 -0.10942383783221032 1104 62-63 -0.11171958114088198 1104 64-65 -0.31869037960375124 1104 66-67 -0.781784111228184 1104 68-69 -0.42407289283114835 1104 70-71 -0.3670212779043638 1104 72-73 -0.37384970021533803 1104 74-75 -0.5800405294753901 1104 76-77 -0.5463790308894971 1104 78-79 -0.5595178022111114 1104 80-81 -0.48829925886313674 1104 82-83 -0.21728575467788858 1104 84-85 -0.22266685917845308 1104 86-87 -0.2478149538874348 1104 88-89 -0.3239389762756488 1104 90-91 -0.3864931842549524 1104 92-93 -0.46305432920483725 1104 94-95 -0.286169358779091 1104 96-97 -0.2387620660454317 1104 98-99 -0.3836819536008633 1104 100 -0.42117822431907825 1105 1 1.3512046396922965 1105 2 0.3995884295228542 1105 3 -0.05918992941886003 1105 4 -0.3034745530082308 1105 5 -1.0982113216582476 1105 6 0.5991165220027455 1105 7 0.3257811276213829 1105 8 0.27435950504520434 1105 9 0.300743748632037 1105 10-11 0.08182325487806708 1105 12-13 0.10515800380589724 1105 14-15 -0.029984975808417857 1105 16-17 0.17847093190804486 1105 18-19 0.11405823666381565 1105 20-21 0.23890288981137076 1105 22-23 0.09571723770572049 1105 24-25 0.17057325052431338 1105 26-27 0.030033370760250477 1105 28-29 0.06909712789105527 1105 30-31 0.040339094565496225 1105 32-33 0.09690842561225566 1105 34-35 0.2412713684777188 1105 36-37 -0.07647360986447893 1105 38-39 0.1957783062254066 1105 40-41 0.19847652465551135 1105 42-43 0.27562708968817873 1105 44-45 0.11556997263079438 1105 46-47 -0.09730874095810549 1105 48-49 -0.015338821679982573 1105 50-51 0.004885590625050895 1105 52-53 -0.08533861439802592 1105 54-55 -0.08661029629352868 1105 56-57 0.07122997989652191 1105 58-59 -0.05192710038333104 1105 60-61 -0.00793001901340773 1105 62-63 -0.23952014496463647 1105 64-65 -0.17372965447984257 1105 66-67 0.005588306247773289 1105 68-69 -0.11410841360435597 1105 70-71 -0.13871423355666934 1105 72-73 -0.023391108370397262 1105 74-75 -0.1337112660125115 1105 76-77 -0.13567170740162737 1105 78-79 0.016896823207027012 1105 80-81 -0.20496180905385586 1105 82-83 -0.12295044646266007 1105 84-85 -0.1746038715531384 1105 86-87 -0.007241940436102823 1105 88-89 0.033928194387286226 1105 90-91 -0.030667056807629933 1105 92-93 -0.06137471463016908 1105 94-95 0.19750629905121997 1105 96-97 -0.05174768958682563 1105 98-99 -0.14022299281291595 1105 100 -0.21489504485571587 1106 1 1.3715575262423663 1106 2 -0.641194730978615 1106 3 -0.05988641603084055 1106 4 -0.2010136336459034 1106 5 -1.2209477401693114 1106 6 -0.5695106254976423 1106 7 0.13228166933318874 1106 8 -0.14276632686936352 1106 9 -0.10105937781897012 1106 10-11 -0.01771629008401021 1106 12-13 -0.0010291189305249304 1106 14-15 -0.012081400510481899 1106 16-17 0.0911469882460807 1106 18-19 0.04951327458363863 1106 20-21 0.010996992891392665 1106 22-23 0.042838426376199834 1106 24-25 0.01947821879547007 1106 26-27 -0.05630388353475979 1106 28-29 -0.06612574782478475 1106 30-31 0.028587817673376037 1106 32-33 -0.09554630275795972 1106 34-35 -0.21118955088461178 1106 36-37 -0.3437155228810411 1106 38-39 -0.14903286851534858 1106 40-41 -0.08295823776516187 1106 42-43 0.14332558837258347 1106 44-45 0.04257817569533273 1106 46-47 0.003770813290465469 1106 48-49 -0.0311684146222575 1106 50-51 -0.17774093777612165 1106 52-53 0.05073939210252121 1106 54-55 -0.10584880426443277 1106 56-57 0.008232765842969059 1106 58-59 -0.12050394607285142 1106 60-61 -0.291574191742086 1106 62-63 -0.10285012484391842 1106 64-65 -0.3939780680381233 1106 66-67 -0.32276643759993107 1106 68-69 -0.3545588082801032 1106 70-71 -0.6100189851431139 1106 72-73 -0.2895612058785204 1106 74-75 -0.43145929263377525 1106 76-77 -0.39229219958549777 1106 78-79 -0.29593787730372156 1106 80-81 -0.4479272942728656 1106 82-83 -0.3326973896603107 1106 84-85 -0.45784949916496487 1106 86-87 -0.4158396807239839 1106 88-89 -0.43688901657076684 1106 90-91 -0.5606585441934797 1106 92-93 -0.45264928513009295 1106 94-95 -0.420571397899721 1106 96-97 -0.337253029317516 1106 98-99 -0.6792804142647029 1106 100 -0.7166517388659344 1107 1 1.277065228147448 1107 2 -0.6643844233822822 1107 3 -0.18552824145432467 1107 4 -0.3555256063893175 1107 5 -1.3110372358264932 1107 6 -0.48911449324561573 1107 7 -0.6965644845129617 1107 8 -0.5896471752785928 1107 9 -0.4366584948591381 1107 10-11 -0.2646301295196807 1107 12-13 -0.3391787422329813 1107 14-15 -0.49603336557598254 1107 16-17 -0.3212354437324265 1107 18-19 -0.37113250976301515 1107 20-21 -0.2714061934419263 1107 22-23 -0.21418686274515863 1107 24-25 -0.4195111760891166 1107 26-27 -0.31496312822019945 1107 28-29 -0.3903939468746387 1107 30-31 -0.6244119663853596 1107 32-33 -0.37138487466640413 1107 34-35 -0.21064549886675366 1107 36-37 -0.33030137153020434 1107 38-39 -0.17944819554639935 1107 40-41 -0.30817777805020796 1107 42-43 -0.22787984374986792 1107 44-45 -0.2554585583130162 1107 46-47 -0.42730733345361926 1107 48-49 -0.37077924047762423 1107 50-51 -0.34446618850792277 1107 52-53 -0.38590823974162447 1107 54-55 -0.4339649566709767 1107 56-57 -0.38565129769174433 1107 58-59 -0.538938731811129 1107 60-61 -0.34096775669242163 1107 62-63 -0.4902685470330752 1107 64-65 -0.5769025125892 1107 66-67 -0.4650507362706904 1107 68-69 -0.7755686935912962 1107 70-71 -0.6151973766206353 1107 72-73 -0.5640783372187954 1107 74-75 -0.5729668025910755 1107 76-77 -0.6009196529181864 1107 78-79 -0.8092920449701211 1107 80-81 -0.7769042125510879 1107 82-83 -0.43424017002405435 1107 84-85 -0.6379289175631584 1107 86-87 -0.4613079133646991 1107 88-89 -0.4059110425796959 1107 90-91 -0.24048783062757195 1107 92-93 -0.3119347594810691 1107 94-95 -0.3815292173728224 1107 96-97 -0.7520683995368174 1107 98-99 -0.7324337565556007 1107 100 -0.7968727187594027 1108 1 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18-19 0.01045193399496469 2223 20-21 -0.15105558475603686 2223 22-23 0.07668823144787495 2223 24-25 0.193222668343779 2223 26-27 0.1770503560091896 2223 28-29 0.07616928297507286 2223 30-31 0.04361113840408848 2223 32-33 0.26871467741941757 2223 34-35 0.104586098974643 2223 36-37 0.12258661014135441 2223 38-39 0.1444576441873835 2223 40-41 0.12087790465618298 2223 42-43 0.18163283311070444 2223 44-45 0.11853603348598796 2223 46-47 0.1962584167909256 2223 48-49 0.015335536946004424 2223 50-51 -0.030780877472011525 2223 52-53 0.19520307418677874 2223 54-55 0.17139287153030125 2223 56-57 0.2612517993282424 2223 58-59 0.32321094032692343 2223 60-61 0.3656920414887779 2223 62-63 0.23117429125445454 2223 64-65 0.20929949576999718 2223 66-67 0.20315431902822212 2223 68-69 0.31821070516464545 2223 70-71 0.39349149151848906 2223 72-73 0.346643345197414 2223 74-75 0.37610051090673124 2223 76-77 0.40328422292019184 2223 78-79 0.46623689861703355 2223 80-81 0.246805518466374 2223 82-83 0.2737596848835864 2223 84-85 0.13014385796022765 2223 86-87 0.31778398392862073 2223 88-89 0.41915670619520995 2223 90-91 0.23111648142398877 2223 92-93 0.42781224155329767 2223 94-95 0.5945305419610563 2223 96-97 0.5088383926581486 2223 98-99 0.4928195115329643 2223 100 0.5566430799272766 2224 1 1.3660630207478608 2224 2 -0.7950408848247701 2224 3 0.10220149605707185 2224 4 -0.39332132595359504 2224 5 -1.0231455423671143 2224 6 0.8403794843924679 2224 7 0.6663476033991245 2224 8 0.4929479588449226 2224 9 0.5115779848183877 2224 10-11 0.4493166769489605 2224 12-13 0.3794653865639859 2224 14-15 0.42967684124776184 2224 16-17 0.4458173179164078 2224 18-19 0.3712165712869364 2224 20-21 0.4593486412430394 2224 22-23 0.46289337143114295 2224 24-25 0.41563206494932103 2224 26-27 0.36787194064106643 2224 28-29 0.3344237027246635 2224 30-31 0.31979660888217154 2224 32-33 0.3756075433958799 2224 34-35 0.40392033922528015 2224 36-37 0.2958449166793997 2224 38-39 0.2056374611549785 2224 40-41 0.3321516523447272 2224 42-43 0.5122816323286301 2224 44-45 0.4815891647063282 2224 46-47 0.4194301539498042 2224 48-49 0.3737766403227951 2224 50-51 0.2511052160700302 2224 52-53 0.42244268880581615 2224 54-55 0.5936017451861133 2224 56-57 0.6477932054034099 2224 58-59 0.6492762737073718 2224 60-61 0.4076016324337388 2224 62-63 0.6301169081231208 2224 64-65 0.4964065473464956 2224 66-67 0.5019588371253434 2224 68-69 0.632529103807812 2224 70-71 0.7638821137579868 2224 72-73 0.5530212117038928 2224 74-75 0.4608483996739139 2224 76-77 0.6796858223925284 2224 78-79 0.4982928919270435 2224 80-81 0.6108639145183474 2224 82-83 0.6170278850649709 2224 84-85 0.29737028105481755 2224 86-87 0.44569878081447456 2224 88-89 0.4867373570556097 2224 90-91 0.6275282689933306 2224 92-93 0.3696034621226545 2224 94-95 0.6154176130892921 2224 96-97 0.5185162014517104 2224 98-99 0.4957195857352943 2224 100 0.5212603490461589 2225 1 1.2787480964586067 2225 2 1.7314923852912418 2225 3 0.7519680320879445 2225 4 1.0597398127984228 2225 5 3.035811611893152 2225 6 -0.17550266731710096 2225 7 0.09705921838163079 2225 8 0.2841159388714516 2225 9 0.13296604514692234 2225 10-11 0.09311532777928733 2225 12-13 0.050313899056405376 2225 14-15 0.0855838097571393 2225 16-17 0.006455982910615887 2225 18-19 0.08483760344974911 2225 20-21 0.05874007449574492 2225 22-23 0.113811773073202 2225 24-25 0.13284913242580387 2225 26-27 -0.011610687599073799 2225 28-29 -0.022941365303971395 2225 30-31 0.1418113909324461 2225 32-33 0.08342243467609478 2225 34-35 -0.055879896039179755 2225 36-37 0.10534792050245301 2225 38-39 -0.06708446465269446 2225 40-41 0.061105835790989715 2225 42-43 0.2715555233747793 2225 44-45 0.17849951814316256 2225 46-47 0.13652673841394147 2225 48-49 -0.009565345381282953 2225 50-51 0.007706574842330838 2225 52-53 0.06779186447995045 2225 54-55 0.11713059209016308 2225 56-57 0.25736567826097456 2225 58-59 0.4032855222414682 2225 60-61 0.26575426625540643 2225 62-63 0.13494087989138137 2225 64-65 0.27108230850505777 2225 66-67 0.32665950510837405 2225 68-69 0.3791845373966112 2225 70-71 0.48119460738124076 2225 72-73 0.44186655532804764 2225 74-75 0.30485695801890245 2225 76-77 0.4715681481497853 2225 78-79 0.36849352690102677 2225 80-81 0.16805928605013776 2225 82-83 0.0803833444608415 2225 84-85 0.032731219952076174 2225 86-87 0.3644818273692323 2225 88-89 0.212039989977022 2225 90-91 0.3378522977772178 2225 92-93 0.2753308314218401 2225 94-95 0.4743302051395162 2225 96-97 0.4203084425257657 2225 98-99 0.603186081555144 2225 100 0.35158905911271177 2226 1 1.4860027291977396 2226 2 1.20700407406461 2226 3 0.7464254688956693 2226 4 0.8635853188035156 2226 5 3.0804103300177843 2226 6 -0.34128475571047545 2226 7 0.14490060619621659 2226 8 0.2994036252236256 2226 9 0.4329253016027579 2226 10-11 0.31476245986312534 2226 12-13 0.1685691085704022 2226 14-15 0.2157498254378396 2226 16-17 0.13975357681967893 2226 18-19 0.1365401701729283 2226 20-21 0.2461702857887076 2226 22-23 0.26978868886796903 2226 24-25 0.34702671482939706 2226 26-27 0.2895987981705659 2226 28-29 0.1813343649047141 2226 30-31 0.1834025329196436 2226 32-33 0.1867670683129674 2226 34-35 0.2585054874470387 2226 36-37 0.1217382646728069 2226 38-39 0.2554577343397426 2226 40-41 0.28888536667876963 2226 42-43 0.2990910433683922 2226 44-45 0.2071605147416662 2226 46-47 0.2422596883516306 2226 48-49 0.439267128394377 2226 50-51 0.1364399610391871 2226 52-53 0.28227404766136033 2226 54-55 0.37622050081201763 2226 56-57 0.4036467114355915 2226 58-59 0.35345484664625104 2226 60-61 0.5011456639931637 2226 62-63 0.37826360749301813 2226 64-65 0.3233628510757569 2226 66-67 0.3640195636445682 2226 68-69 0.46637256930327453 2226 70-71 0.6013602897875643 2226 72-73 0.40403946228234133 2226 74-75 0.5083080593105933 2226 76-77 0.7412612865105928 2226 78-79 0.6683494983222928 2226 80-81 0.49657910641825254 2226 82-83 0.3984060725728966 2226 84-85 0.30309541079039093 2226 86-87 0.5058571216490151 2226 88-89 0.22360431088242905 2226 90-91 0.4682697704825003 2226 92-93 0.38470232697413564 2226 94-95 0.41507431976908293 2226 96-97 0.3796446139957297 2226 98-99 0.520594834181189 2226 100 0.18654437782661404 2227 1 1.1683360901442192 2227 2 1.6879186214224067 2227 3 0.686192162924451 2227 4 0.05239597044863231 2227 5 3.1128321785421136 2227 6 0.23996099876850252 2227 7 0.28144921411717405 2227 8 0.5281730077686007 2227 9 0.26957587733682686 2227 10-11 0.29302659716572776 2227 12-13 0.3362334130580322 2227 14-15 0.23278161318815194 2227 16-17 0.2319267564231069 2227 18-19 0.15060389629069704 2227 20-21 0.14776351404147192 2227 22-23 0.2129136935747553 2227 24-25 0.35122968650584596 2227 26-27 0.18123864243243304 2227 28-29 0.2814634438055066 2227 30-31 0.27240160662415036 2227 32-33 0.1569796644255419 2227 34-35 0.2765494224708007 2227 36-37 0.22625587558351157 2227 38-39 0.21708108602325638 2227 40-41 0.25407322392383236 2227 42-43 0.4029492523798197 2227 44-45 0.33360482028362526 2227 46-47 0.4075755701774071 2227 48-49 0.2838391120976027 2227 50-51 0.2721356240182473 2227 52-53 0.32755709494762186 2227 54-55 0.3232359466011374 2227 56-57 0.337389020547171 2227 58-59 0.35694524856812393 2227 60-61 0.11621370529238817 2227 62-63 0.2537365077016247 2227 64-65 0.13905444739165773 2227 66-67 0.23925072332134079 2227 68-69 0.30222427165365673 2227 70-71 0.322038820065373 2227 72-73 0.29630286156088914 2227 74-75 0.2517275205530396 2227 76-77 0.5065193313493239 2227 78-79 0.38176421512439873 2227 80-81 0.20369697187194902 2227 82-83 0.039145904032295675 2227 84-85 0.17842778519450775 2227 86-87 0.3743891315596244 2227 88-89 0.35177574332686135 2227 90-91 0.37298363554458547 2227 92-93 0.32148062605460836 2227 94-95 0.5365804913069638 2227 96-97 0.284837893458338 2227 98-99 0.3298344434802871 2227 100 0.21070788780876626 2228 1 1.626730064547436 2228 2 1.34333880777179 2228 3 -0.1092226219935526 2228 4 -0.11012259697173477 2228 5 3.1109131700048493 2228 6 0.11183814621257682 2228 7 0.3848977279733461 2228 8 0.5177797535562334 2228 9 0.40446702349056096 2228 10-11 0.3408964826853875 2228 12-13 0.25883120147196337 2228 14-15 0.23487916904045392 2228 16-17 0.27762436628972154 2228 18-19 0.13340618889824185 2228 20-21 0.36084293008672574 2228 22-23 0.2589199352287963 2228 24-25 0.40673764611513974 2228 26-27 0.2883391240600588 2228 28-29 0.236842669300259 2228 30-31 0.3622965787917778 2228 32-33 0.3747887838421846 2228 34-35 0.44557296370923183 2228 36-37 0.5736105245654954 2228 38-39 0.3681574712653486 2228 40-41 0.39634017466577376 2228 42-43 0.5617132248325163 2228 44-45 0.4879496716692415 2228 46-47 0.5377973289434834 2228 48-49 0.451398115233431 2228 50-51 0.3904571292171255 2228 52-53 0.44095339496712427 2228 54-55 0.5916843854374321 2228 56-57 0.451525316665645 2228 58-59 0.6066120102599726 2228 60-61 0.5056632094109759 2228 62-63 0.6951371288662287 2228 64-65 0.4447082456482008 2228 66-67 0.38091241366928585 2228 68-69 0.5681437903261042 2228 70-71 0.4967754252657528 2228 72-73 0.6474827742377443 2228 74-75 0.4167768086143724 2228 76-77 0.6205211918423572 2228 78-79 0.7663663846993316 2228 80-81 0.5576352155065081 2228 82-83 0.4847926504200899 2228 84-85 0.5191752832815055 2228 86-87 0.5001521587618285 2228 88-89 0.5496256735583742 2228 90-91 0.7543887579020137 2228 92-93 0.6681856028734714 2228 94-95 0.8219054024084045 2228 96-97 0.6513992822263575 2228 98-99 0.7780369671490561 2228 100 0.8466109021075496 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 12.0 13 20.0 14 126.0 15 351.0 16 1405.0 17 3567.0 18 5863.0 19 7442.0 20 8750.0 21 10283.0 22 11788.0 23 13388.0 24 14993.0 25 17029.0 26 18970.0 27 21565.0 28 24468.0 29 27838.0 30 31666.0 31 36620.0 32 42357.0 33 50880.0 34 61957.0 35 79931.0 36 111888.0 37 186111.0 38 451020.0 39 795605.0 40 746.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.110136630922597 20.00342283473856 19.258208572484065 34.62823196185478 2 20.934987594026865 51.793624316787614 10.090551573542895 17.18083651564262 3 39.6640054206929 15.092358001414095 23.904519208107473 21.33911736978553 4 18.021005185010605 16.41649972503732 22.383337261371672 43.17915782858041 5 16.96210425013748 35.52895946264435 26.758631864246997 20.75030442297117 6 17.20564262707204 27.98491633278341 31.000520465079738 23.808920575064814 7 20.23141055856705 26.61255793856548 36.619873909969364 16.536157592898107 8 20.310020425799355 27.704061591641132 35.0539123261843 16.932005656375207 9 20.692120355094666 28.178126718516772 27.79298255950978 23.33677036687878 10-11 19.370212703275985 30.126482834472462 28.90591857176526 21.59738589048629 12-13 24.414550436012256 26.34361006363422 27.41738844371121 21.824451056642314 14-15 21.735235485898343 27.333328423285412 28.597101107706813 22.334334983109436 16-17 21.00074141723623 29.856773902113286 28.073051692984524 21.06943298766596 18-19 21.44573906041323 29.66496288003771 29.864286275434047 19.025011784115012 20-21 22.712040419514494 29.2819054913976 29.864752729986645 18.141301359101263 22-23 22.109798491633278 30.48366427056328 28.64777280226255 18.758764435540893 24-25 22.25496405844921 28.39323591798256 26.435599811454157 22.91620021211407 26-27 20.043674876266792 26.248134181789613 30.17423305051457 23.53395789142902 28-29 19.233730377539793 28.342956241993527 30.858824343904868 21.564489036561817 30-31 19.9588647617046 30.33565460738485 28.296472273980232 21.409008356930322 32-33 20.344218909576558 30.271255597454633 28.91389739963862 20.47062809333019 34-35 21.24621926310001 29.599290989080053 29.890481381098276 19.26400836672166 36-37 21.75752710346453 28.037110142194987 28.903414447325005 21.301948307015476 38-39 21.555286918871992 29.311025468165163 28.945493540526197 20.188194072436648 40-41 20.82848970310705 29.886804515053083 29.97877108989229 19.305934691947574 42-43 20.38929362736373 30.162117706268297 29.520692808612235 19.927895857755736 44-45 20.62198032052793 30.034198483777203 28.759697344646085 20.584123851048787 46-47 19.728248394077884 28.115206575657254 28.953293536662162 23.203251493602703 48-49 20.720279479927726 27.99576753869118 30.485652839971717 20.798300141409378 50-51 21.670987508838085 30.821966572393745 28.284822059863302 19.22222385890486 52-53 20.298141403577254 30.591041273091292 28.12022876165996 20.990588561671498 54-55 22.29644981166175 28.48158115733488 28.30957210138344 20.912396929619934 56-57 21.822927689940784 28.631990300582743 29.564464147954418 19.98061786152206 58-59 21.26088481007934 28.060244833336935 29.05080429244069 21.628066064143034 60-61 19.344006489132077 30.48330678628876 29.842396972262105 20.330289752317057 62-63 20.511628112537757 31.436416501465086 27.23827477986899 20.813680606128166 64-65 19.789786015096443 30.25160570921013 29.079797647005677 20.878810628687756 66-67 20.223505381412522 30.27611654489748 29.701469086338285 19.798908987351716 68-69 20.76714588734386 29.907641998585905 29.471605192866683 19.85360692120355 70-71 19.618685678372223 30.925863186424703 29.316619530206616 20.138831604996465 72-73 20.014779244245425 30.815534409615836 28.653370256893705 20.51631608924503 74-75 19.809932044936758 29.155668159321234 30.83242497446775 20.201974821274256 76-77 19.323960051850104 30.568730850813104 28.703477295938406 21.403831801398383 78-79 19.287748448424857 29.647556760153982 28.584555935265925 22.480138856155236 80-81 20.54419203450399 29.503214617844936 28.542045355228286 21.410547992422792 82-83 19.92458166391704 28.410494736428625 30.3493008091759 21.315622790478436 84-85 20.198120433655433 29.17918728886794 28.435634181789617 22.187058095687014 86-87 21.683017126247154 28.575472346610102 29.36999175111949 20.371518776023255 88-89 22.628643255558174 27.709830897949566 28.97492929530992 20.68659655118234 90-91 22.897100125697225 27.834276062534368 28.412630607274725 20.855993204493675 92-93 22.102580721187838 27.939277437347787 29.648391468300733 20.30975037316364 94-95 22.237312436169375 28.224624872338754 29.33363284625658 20.20442984523529 96-97 21.87141566501689 28.170049689684973 29.256986998193103 20.701547647105038 98-99 22.891920025139445 28.46644473250059 28.68175033388326 19.959884908476706 100 22.946225155157514 30.015810354309057 27.124725037316367 19.913239453217063 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 6.0 2 10.0 3 14.0 4 21.0 5 25.0 6 27.0 7 36.0 8 47.0 9 47.0 10 51.5 11 68.5 12 80.5 13 92.0 14 107.0 15 126.5 16 167.5 17 240.0 18 364.0 19 584.5 20 957.0 21 1488.0 22 2252.5 23 3463.5 24 5361.0 25 8129.0 26 11689.0 27 15658.5 28 19873.0 29 24762.0 30 30454.0 31 36774.5 32 43869.5 33 51749.0 34 60565.0 35 70465.0 36 80111.0 37 86841.5 38 90965.5 39 94413.0 40 96965.0 41 97310.0 42 95520.5 43 93983.5 44 94759.0 45 93781.5 46 86668.5 47 78286.0 48 73238.5 49 70645.0 50 68073.0 51 63219.5 52 55498.5 53 46908.5 54 39402.0 55 32921.0 56 26193.5 57 19147.0 58 13353.5 59 9468.0 60 7290.0 61 5935.0 62 4791.0 63 3752.5 64 2867.0 65 2429.5 66 2223.0 67 1791.5 68 1330.5 69 1331.0 70 1603.0 71 1487.0 72 919.0 73 428.0 74 223.5 75 144.0 76 103.0 77 75.0 78 71.5 79 72.5 80 47.0 81 26.0 82 19.0 83 16.0 84 14.5 85 13.5 86 14.0 87 9.5 88 12.0 89 12.5 90 9.0 91 10.5 92 8.0 93 7.5 94 9.5 95 10.0 96 10.0 97 14.0 98 36.0 99 70.0 100 74.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.348053657003693 2 0.005941157985701941 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.4370335454474035E-4 30-31 0.009157239374656297 32-33 0.0 34-35 0.0 36-37 0.0 38-39 9.329091051928667E-4 40-41 0.0014975646162306545 42-43 1.2275119805169298E-4 44-45 0.0 46-47 0.0018412679707753947 48-49 0.0 50-51 0.0 52-53 0.001104760782465237 54-55 0.020131196480477648 56-57 0.011244009741535077 58-59 0.015515751433733993 60-61 1.9640191688270877E-4 62-63 0.01173501453374185 64-65 4.910047922067719E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.9460287532406316E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2036640.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.2536863042966 #Duplication Level Percentage of deduplicated Percentage of total 1 87.10086903810648 66.41762344463392 2 8.134000887714771 12.404951041813444 3 2.1753768766134907 4.97641517828717 4 0.9210709606804159 2.809402243988862 5 0.5025049860351029 1.9158928785732838 6 0.29501141088752963 1.349742454920338 7 0.18930879135461653 1.0104845233420323 8 0.13936377678275425 0.8501601373579325 9 0.09903379388545697 0.6796522667219401 >10 0.41678107215611104 5.394917679935554 >50 0.018890578151948865 0.9888498912223185 >100 0.007526262958366586 1.061842597025873 >500 2.615646728657016E-4 0.14006566217745242 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.910047922067719E-5 0.0 0.0 0.0 0.0 8 4.910047922067719E-5 0.0 0.0 0.0 0.0 9 4.910047922067719E-5 0.0 0.0 0.0 0.0 10-11 4.910047922067719E-5 0.0 0.0 0.0 0.0 12-13 4.910047922067719E-5 0.0 0.0 0.0 0.0 14-15 4.910047922067719E-5 0.0 0.0 0.0 0.0 16-17 4.910047922067719E-5 0.0 0.0 0.0 0.0 18-19 4.910047922067719E-5 0.0 0.0 0.0 0.0 20-21 4.910047922067719E-5 0.0 0.0 4.910047922067719E-5 0.0 22-23 4.910047922067719E-5 0.0 0.0 4.910047922067719E-5 0.0 24-25 4.910047922067719E-5 0.0 0.0 4.910047922067719E-5 0.0 26-27 4.910047922067719E-5 0.0 0.0 4.910047922067719E-5 0.0 28-29 4.910047922067719E-5 0.0 0.0 4.910047922067719E-5 0.0 30-31 4.910047922067719E-5 0.0 0.0 4.910047922067719E-5 0.0 32-33 4.910047922067719E-5 0.0 0.0 4.910047922067719E-5 0.0 34-35 4.910047922067719E-5 0.0 0.0 4.910047922067719E-5 0.0 36-37 4.910047922067719E-5 0.0 0.0 4.910047922067719E-5 0.0 38-39 4.910047922067719E-5 0.0 0.0 4.910047922067719E-5 0.0 40-41 7.365071883101579E-5 0.0 0.0 4.910047922067719E-5 0.0 42-43 9.820095844135439E-5 0.0 0.0 4.910047922067719E-5 0.0 44-45 9.820095844135439E-5 0.0 0.0 4.910047922067719E-5 0.0 46-47 1.4730143766203158E-4 0.0 0.0 4.910047922067719E-5 0.0 48-49 1.4730143766203158E-4 0.0 0.0 4.910047922067719E-5 0.0 50-51 1.4730143766203158E-4 0.0 0.0 9.820095844135439E-5 0.0 52-53 1.4730143766203158E-4 0.0 0.0 9.820095844135439E-5 0.0 54-55 1.4730143766203158E-4 0.0 0.0 9.820095844135439E-5 0.0 56-57 2.2095215649304737E-4 0.0 0.0 9.820095844135439E-5 0.0 58-59 2.4550239610338597E-4 0.0 0.0 9.820095844135439E-5 0.0 60-61 2.7005263571372456E-4 0.0 0.0 9.820095844135439E-5 0.0 62-63 3.4370335454474035E-4 0.0 0.0 9.820095844135439E-5 0.0 64-65 3.9280383376541755E-4 0.0 0.0 9.820095844135439E-5 0.0 66-67 5.155550318171106E-4 0.0 0.0 9.820095844135439E-5 0.0 68-69 7.365071883101579E-4 0.0 0.0 9.820095844135439E-5 0.0 70-71 8.101579071411737E-4 0.0 0.0 9.820095844135439E-5 0.0 72-73 8.838086259721895E-4 0.0 0.0 9.820095844135439E-5 0.0 74-75 9.820095844135439E-4 0.0 0.0 9.820095844135439E-5 0.0 76-77 0.0010311100636342212 0.0 0.0 9.820095844135439E-5 0.0 78-79 0.001104760782465237 0.0 0.0 9.820095844135439E-5 0.0 80-81 0.0013748134181789614 0.0 0.0 9.820095844135439E-5 0.0 82-83 0.0017185167727237017 0.0 0.0 9.820095844135439E-5 0.0 84-85 0.0018658182103857336 0.0 0.0 9.820095844135439E-5 0.0 86-87 0.0019149186896064106 4.910047922067719E-5 0.0 9.820095844135439E-5 0.0 88 0.0019149186896064106 4.910047922067719E-5 0.0 9.820095844135439E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 8585 0.0 80.47742 8 CACCGGC 8305 0.0 79.79975 9 CTTCACC 9625 0.0 74.70756 6 GTGTGGC 3845 0.0 74.340836 7 CCTTCAC 10710 0.0 70.95187 5 CGGGGCA 925 0.0 70.53092 94 CGTAGTG 1705 0.0 66.89419 4 TGTGGCA 4350 0.0 66.573654 8 AACGGGC 635 0.0 65.04523 5 TTCACCG 11100 0.0 64.39964 7 GCAGCCT 50 1.9821528E-7 63.634716 1 ATGCTGC 30 1.380348E-4 62.58447 2 TGTGTGG 4410 0.0 62.368534 6 CGGGCCC 675 0.0 61.886047 7 ACCTTCA 4260 0.0 61.259254 4 GGCCCGA 685 0.0 60.982597 9 GGGCCCG 690 0.0 60.540695 8 ATGTGTG 4300 0.0 59.92532 5 GCCTTCA 3210 0.0 59.072155 4 GGCTGCC 45 7.898501E-6 58.921032 1 >>END_MODULE