FastQCFastQC Report
Mon 6 Mar 2017
1NF_1A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_1A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1355760
Sequences flagged as poor quality0
Sequence length96
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA92500.6822741488168997No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG35250.2600017702248185No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA34540.25476485513660235No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA33550.24746267776007552No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG30580.22555614563049506No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT28100.20726382250545822No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG27810.2051248008497079No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT27660.20401841033811297No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG26800.19767510473830177No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT24370.1797515784504632No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC24370.1797515784504632No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA23070.1701628606833068No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA21990.16219684899982298No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA21370.15762376821856375No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA20760.1531244468047442No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA19420.14324069156782912No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC17550.1294476898566118No Hit
CAGCACTTTGAACTTTGTTCACCATTTCTTCGTAGTCGTTGATACGTTGT17210.1269398713636632No Hit
CAGCTCTGTTATAAAACAAGTTCTTGGATTTTTTTCATCATTTGAACATC15720.11594972561515313No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC15270.11263055408036819No Hit
CAGCCTTGACCTTGTCATAATCTGAACTCTCGCCTATGGCTAGCGCTGAG15240.11240927597804921No Hit
CAGCTAAAAATATCAGATTAGTTCCGAAATTTCAGGAAAAAGCTATTGAT15040.11093408862925593No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTTAAAAAAAAGCCATGAGTGCTC14710.10850002950374697No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTT158.2045444E-490.000013
GCTGCCG158.2045444E-490.000013
GCTGCCA158.2045444E-490.000013
GCTGCAG301.7935236E-890.000013
GCAGCAG158.2045444E-490.000013
GCAGCAA600.090.000013
GCTGCCC850.090.03
GCTGCAT256.375976E-790.03
TGCTGCA256.375976E-790.02
CTGCAGC450.090.01
AGCTGCC1400.090.02
CAGCTGC3450.090.01
GCAGCCA401.4551915E-1190.03
TGCAGCA202.2808279E-590.02
AGCAGCC700.090.02
AGCTGCT550.089.999992
AGCTGCG550.089.999992
AGCAGCA1200.086.250012
AGCTGCA1000.085.52
CAGCCCT89550.084.3718641
Produced by FastQC (version 0.11.5)