##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1NF_1A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1355760 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.807694577211306 12.0 12.0 12.0 2.0 32.0 2 14.637133415943826 12.0 12.0 12.0 12.0 22.0 3 13.76385200920517 12.0 12.0 12.0 12.0 22.0 4 13.678903345724907 12.0 12.0 12.0 12.0 22.0 5 22.41600283235971 22.0 22.0 22.0 22.0 27.0 6 26.69697365315395 27.0 22.0 32.0 22.0 32.0 7 34.85263468460494 37.0 37.0 37.0 27.0 41.0 8 37.45248126512067 41.0 37.0 41.0 27.0 41.0 9 38.44348262229303 41.0 37.0 41.0 32.0 41.0 10-11 38.82741045612793 41.0 41.0 41.0 34.5 41.0 12-13 39.00059523809524 41.0 41.0 41.0 37.0 41.0 14-15 38.949467826163925 41.0 41.0 41.0 37.0 41.0 16-17 38.85523212072933 41.0 41.0 41.0 37.0 41.0 18-19 38.787797250250776 41.0 41.0 41.0 34.5 41.0 20-21 38.80621275151944 41.0 41.0 41.0 37.0 41.0 22-23 38.79458458724258 41.0 41.0 41.0 34.5 41.0 24-25 38.808165161975566 41.0 41.0 41.0 34.5 41.0 26-27 38.774283427745324 41.0 41.0 41.0 34.5 41.0 28-29 38.72478536024075 41.0 41.0 41.0 32.0 41.0 30-31 38.59867380657343 41.0 41.0 41.0 32.0 41.0 32-33 38.635369091874665 41.0 41.0 41.0 32.0 41.0 34-35 38.61341240337523 41.0 41.0 41.0 32.0 41.0 36-37 38.5207418717177 41.0 41.0 41.0 32.0 41.0 38-39 38.42980579158553 41.0 41.0 41.0 32.0 41.0 40-41 38.47751482563285 41.0 41.0 41.0 32.0 41.0 42-43 38.216480055467045 41.0 41.0 41.0 32.0 41.0 44-45 38.32877869239394 41.0 41.0 41.0 32.0 41.0 46-47 38.32300296512657 41.0 41.0 41.0 32.0 41.0 48-49 38.29663841682894 41.0 41.0 41.0 32.0 41.0 50-51 38.16091675517791 41.0 41.0 41.0 32.0 41.0 52-53 38.15470990440786 41.0 39.0 41.0 32.0 41.0 54-55 38.00339551248008 41.0 37.0 41.0 32.0 41.0 56-57 37.77832138431581 41.0 37.0 41.0 27.0 41.0 58-59 37.773069717354105 41.0 37.0 41.0 27.0 41.0 60-61 37.76978152475364 41.0 37.0 41.0 27.0 41.0 62-63 37.77147983418894 41.0 37.0 41.0 27.0 41.0 64-65 37.77587257331681 41.0 37.0 41.0 27.0 41.0 66-67 37.63932296276627 41.0 37.0 41.0 27.0 41.0 68-69 37.65609621171889 41.0 37.0 41.0 27.0 41.0 70-71 37.65665309494305 41.0 37.0 41.0 27.0 41.0 72-73 37.48860934088629 41.0 37.0 41.0 27.0 41.0 74-75 37.41741384905883 41.0 37.0 41.0 27.0 41.0 76-77 36.34507766861391 39.0 34.5 41.0 24.5 41.0 78-79 36.87180105623414 41.0 37.0 41.0 27.0 41.0 80-81 37.5349103086092 41.0 37.0 41.0 27.0 41.0 82-83 37.72236752817608 41.0 37.0 41.0 27.0 41.0 84-85 37.65238021478728 41.0 37.0 41.0 27.0 41.0 86-87 37.519303564052635 41.0 37.0 41.0 27.0 41.0 88-89 37.40927118369032 41.0 37.0 41.0 27.0 41.0 90-91 37.23829844515254 41.0 37.0 41.0 27.0 41.0 92-93 37.10673091107571 41.0 37.0 41.0 27.0 41.0 94-95 36.932442688971506 41.0 37.0 41.0 27.0 41.0 96-97 36.87961733640172 41.0 37.0 41.0 27.0 41.0 98-99 36.76888866761078 41.0 37.0 41.0 27.0 41.0 100 36.64114371275152 41.0 37.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.5541845736681932 1101 2 -0.5518581622591725 1101 3 0.11791077194826016 1101 4 0.049602152297737234 1101 5 -1.1204786230351402 1101 6 -0.8461188081863469 1101 7 -0.42943290974747583 1101 8 -0.1878365055184048 1101 9 -0.12935462000094589 1101 10-11 -0.13432712651363232 1101 12-13 -0.09928038863893107 1101 14-15 -0.10429210094891772 1101 16-17 -0.22482683919933066 1101 18-19 0.013110496190343213 1101 20-21 -0.08823106158447303 1101 22-23 -0.056841547633389666 1101 24-25 -0.005299416594297668 1101 26-27 0.09867657466320878 1101 28-29 -0.06976150272939918 1101 30-31 0.09326112146812449 1101 32-33 -0.15547574830377897 1101 34-35 0.018775035808424434 1101 36-37 -0.014767805310619053 1101 38-39 -0.07492791810890509 1101 40-41 -0.06806474460709211 1101 42-43 0.0390871863294322 1101 44-45 -0.12812993808648088 1101 46-47 -0.09617321884066143 1101 48-49 0.020056073141631714 1101 50-51 -0.08928906890733401 1101 52-53 -0.11792167616039251 1101 54-55 -0.08297754553498038 1101 56-57 0.1819342400196362 1101 58-59 -0.08875252915493803 1101 60-61 0.04820711079833018 1101 62-63 -0.06270423792820878 1101 64-65 0.02231272788642258 1101 66-67 -0.07335202894904569 1101 68-69 -0.15860773837419373 1101 70-71 -0.36830089795518006 1101 72-73 -0.10597868643554165 1101 74-75 0.23501409949856367 1101 76-77 -0.419969289210826 1101 78-79 -0.2886482376232209 1101 80-81 -0.10187238573543311 1101 82-83 -0.024242292295731716 1101 84-85 -0.23452478086333173 1101 86-87 -0.13930579601876047 1101 88-89 -0.19869630837271757 1101 90-91 0.02118560591590324 1101 92-93 0.03412083089982332 1101 94-95 0.035833510330142815 1101 96-97 -0.1715917950092063 1101 98-99 -0.4161644133989242 1101 100 -0.39186062458473714 1102 1 1.6343678857984507 1102 2 1.0353284625891526 1102 3 -0.05935533294188211 1102 4 0.13985167680303334 1102 5 -0.8361054992973536 1102 6 -0.01316125212415642 1102 7 -0.13286276955525977 1102 8 -0.26998477589329894 1102 9 -0.3009126891355649 1102 10-11 -0.36024904041655503 1102 12-13 -0.2610892418674666 1102 14-15 -0.3102327407359624 1102 16-17 -0.3307540433744478 1102 18-19 -0.2853511262437678 1102 20-21 -0.1310623228945289 1102 22-23 -0.13741323292098429 1102 24-25 -0.09115612579307708 1102 26-27 -0.33895699421319136 1102 28-29 -0.474801116791447 1102 30-31 -0.19737215770289396 1102 32-33 -0.24087165799966925 1102 34-35 -0.09208660677909819 1102 36-37 -0.2227290351729394 1102 38-39 -0.39877549948882063 1102 40-41 -0.10620508797094175 1102 42-43 -0.1008929961429672 1102 44-45 -0.27716761238205123 1102 46-47 -0.5508500713806157 1102 48-49 -0.48318106960753937 1102 50-51 -0.22034821499022428 1102 52-53 -0.3770180052992913 1102 54-55 -0.4482152089141138 1102 56-57 -0.1705805286569202 1102 58-59 -0.23557542679657217 1102 60-61 -0.3436267662574224 1102 62-63 -0.5222514997924819 1102 64-65 -0.8241192774206496 1102 66-67 -0.448209800400889 1102 68-69 -0.4658182534199895 1102 70-71 -0.47691732383049157 1102 72-73 -0.30625747538307024 1102 74-75 -0.571220234367992 1102 76-77 -0.1732977521348431 1102 78-79 -0.45597513865733674 1102 80-81 -0.2849598893567489 1102 82-83 -0.5916218861203859 1102 84-85 -0.33720644503181063 1102 86-87 -0.18217694430173736 1102 88-89 -0.639538679254386 1102 90-91 -0.4959416503222087 1102 92-93 -0.29692949806273816 1102 94-95 -0.29950361856356267 1102 96-97 -0.44383717966657343 1102 98-99 -0.4566507405644771 1102 100 -0.6031902043439885 1103 1 1.2299823841565978 1103 2 0.84582061122744 1103 3 0.2520366706429886 1103 4 0.9133341081718171 1103 5 2.94044094907629 1103 6 -0.15787422240202886 1103 7 -0.24140986390399632 1103 8 0.055290600088937936 1103 9 -0.01155440137694086 1103 10-11 -0.02142710112367041 1103 12-13 -0.33160964464166653 1103 14-15 -0.2762899502690459 1103 16-17 -0.3734836955226797 1103 18-19 -0.09350888965683879 1103 20-21 0.09449173896485519 1103 22-23 0.1298931527756153 1103 24-25 -0.011181426484299095 1103 26-27 -0.06305433858629783 1103 28-29 -0.18392391462015922 1103 30-31 -0.04403745676463444 1103 32-33 -0.10611892533588474 1103 34-35 -0.1769279427836281 1103 36-37 -0.12712944952813388 1103 38-39 -0.06745174520960973 1103 40-41 -0.2256319160149971 1103 42-43 -0.04934956668447654 1103 44-45 -0.354188866999543 1103 46-47 -0.17528502503481747 1103 48-49 -0.4395952094786111 1103 50-51 -0.21864634704557062 1103 52-53 0.05299372118553691 1103 54-55 -0.05526193881068764 1103 56-57 0.009537405444895342 1103 58-59 -0.2851492869196832 1103 60-61 -0.22459603442874254 1103 62-63 -0.2621406801461106 1103 64-65 -0.17875414872756323 1103 66-67 -0.11770624535552088 1103 68-69 -0.05216686464627429 1103 70-71 -0.2543934525774034 1103 72-73 -0.2670741392126885 1103 74-75 -0.19115300349154296 1103 76-77 -0.24339768913772275 1103 78-79 -0.4353123012844833 1103 80-81 -0.1360539005565613 1103 82-83 -0.31420322587855054 1103 84-85 -0.23313971173077874 1103 86-87 0.017393351319164196 1103 88-89 0.0032723744852205527 1103 90-91 -0.3506892762909857 1103 92-93 -0.2946383960786889 1103 94-95 -0.4191344961731076 1103 96-97 -0.05489251965461506 1103 98-99 -0.22692912091286388 1103 100 -0.16522490950146107 1104 1 1.2925616040372532 1104 2 -0.3408088672527434 1104 3 0.10168577053288885 1104 4 -0.055560021769601065 1104 5 -1.0684654110510081 1104 6 -0.4644915855337608 1104 7 -0.14170011549844475 1104 8 0.004827446394834567 1104 9 -0.07981520291346555 1104 10-11 0.059933779794228315 1104 12-13 -0.16115322329167014 1104 14-15 -0.1700696375380275 1104 16-17 0.0655254297462946 1104 18-19 0.03719081478033104 1104 20-21 0.07820707421118556 1104 22-23 -0.03175631459404116 1104 24-25 -0.37861633160705566 1104 26-27 -0.09609593700843533 1104 28-29 -0.14177427284115396 1104 30-31 -0.1285843585226516 1104 32-33 -0.13617869821616324 1104 34-35 -0.3907322626805083 1104 36-37 -0.39032929392342197 1104 38-39 -0.2475046278443216 1104 40-41 -0.3291295051467742 1104 42-43 -0.09266086080177871 1104 44-45 -0.1455134736454653 1104 46-47 -0.3839277614958476 1104 48-49 -0.3753278794344084 1104 50-51 -0.418712522112358 1104 52-53 -0.11641516751833336 1104 54-55 -0.29992307509831306 1104 56-57 -0.14859803668637284 1104 58-59 -0.22747111306443202 1104 60-61 -0.26531307931725934 1104 62-63 -0.2612305935443331 1104 64-65 -0.3109374091630599 1104 66-67 -0.5941552424659235 1104 68-69 -0.38652930878863145 1104 70-71 -0.46337388284452885 1104 72-73 -0.32218856753044633 1104 74-75 -0.294917230772036 1104 76-77 -0.4233012831185192 1104 78-79 -0.358597670852852 1104 80-81 -0.5541077076325536 1104 82-83 -0.4224235041583455 1104 84-85 -0.546852951194083 1104 86-87 -0.575446606937426 1104 88-89 -0.3510283588952916 1104 90-91 -0.5662498910731486 1104 92-93 -0.5045181469388993 1104 94-95 -0.492255074035036 1104 96-97 -0.2719051101429173 1104 98-99 -0.5850076467448275 1104 100 -0.3605339441924116 1105 1 1.2207191775842006 1105 2 0.531709756223357 1105 3 -0.20938590354575837 1105 4 -0.3219563966801662 1105 5 -1.043402631348144 1105 6 0.3445304539999192 1105 7 0.27487372605821037 1105 8 0.020445914149213706 1105 9 0.2561775581308581 1105 10-11 0.012599286731564519 1105 12-13 0.11799619941564998 1105 14-15 0.21074011541156779 1105 16-17 0.05527234687708216 1105 18-19 0.12469309449021182 1105 20-21 -0.009127559141312247 1105 22-23 0.3724930646234199 1105 24-25 0.17002987144846315 1105 26-27 0.22152516819491552 1105 28-29 0.02602500045748002 1105 30-31 0.15574035633824934 1105 32-33 -0.051828731184279775 1105 34-35 0.11746066390582399 1105 36-37 0.054672179119883424 1105 38-39 0.10614992245660204 1105 40-41 0.18305396590441347 1105 42-43 0.39715830559600107 1105 44-45 0.030068378460988754 1105 46-47 0.16468477199835263 1105 48-49 -0.14401799320947362 1105 50-51 -0.16578537462564213 1105 52-53 -0.18848766930702965 1105 54-55 0.023278058443587213 1105 56-57 0.11695519629296314 1105 58-59 0.09119304709044229 1105 60-61 -0.012820128881656956 1105 62-63 -0.1822502200927829 1105 64-65 -0.05655966474309082 1105 66-67 0.008579006492425378 1105 68-69 -0.27002491070218326 1105 70-71 -0.026402600174534996 1105 72-73 -0.18129337806902157 1105 74-75 -0.05419880650750741 1105 76-77 -0.1693079328568743 1105 78-79 -0.08216916956919817 1105 80-81 -0.06350572393423448 1105 82-83 -0.17389093587226512 1105 84-85 -0.05512935023450183 1105 86-87 -0.23055385851919397 1105 88-89 -0.19998600539296518 1105 90-91 -0.14666823537247353 1105 92-93 -0.13650563308527097 1105 94-95 0.16544753956465996 1105 96-97 0.10279081163147197 1105 98-99 -0.28596652779307163 1105 100 -0.44953381793155955 1106 1 1.107412012612949 1106 2 -0.8022962145860184 1106 3 0.05369597394207837 1106 4 -0.18238872750723445 1106 5 -1.1246788918523443 1106 6 -0.6708291766944647 1106 7 -0.005085362840503649 1106 8 -0.2534525268061145 1106 9 -0.3251857766767543 1106 10-11 -0.1634518245024097 1106 12-13 -0.009876561530838046 1106 14-15 -0.06305328352695483 1106 16-17 -0.13177915135378981 1106 18-19 0.01917078688295959 1106 20-21 -0.09195042512981644 1106 22-23 -0.14987121003381532 1106 24-25 -0.3195087729566737 1106 26-27 -0.3130412570593961 1106 28-29 -0.2992845970657356 1106 30-31 -0.15091529934507264 1106 32-33 -0.16678892578843119 1106 34-35 -0.3544958818703847 1106 36-37 -0.36184775122067236 1106 38-39 -0.25493477458010716 1106 40-41 -0.24339450822208164 1106 42-43 -0.13541646773438742 1106 44-45 -0.13132384290124577 1106 46-47 -0.49004211875794823 1106 48-49 -0.40150569223645505 1106 50-51 -0.34425728970747116 1106 52-53 -0.37178184223935773 1106 54-55 -0.2880982684029405 1106 56-57 -0.24462499499407642 1106 58-59 -0.2772024361028329 1106 60-61 -0.19534998292854056 1106 62-63 -0.25627167077832524 1106 64-65 -0.5928834705812136 1106 66-67 -0.551450432914919 1106 68-69 -0.5270026030949353 1106 70-71 -0.5195956010590947 1106 72-73 -0.5969598420230184 1106 74-75 -0.714858498015694 1106 76-77 -0.3770983187480681 1106 78-79 -0.29888328942766407 1106 80-81 -0.6096889669682923 1106 82-83 -0.45290948408102594 1106 84-85 -0.723633330774085 1106 86-87 -0.6430455657283929 1106 88-89 -0.554186994999327 1106 90-91 -0.7434126239173651 1106 92-93 -0.28256379319142155 1106 94-95 -0.46079845225311544 1106 96-97 -0.45200242145124037 1106 98-99 -0.5624398802919686 1106 100 -0.4959803570735275 1107 1 1.3057253699029268 1107 2 -0.5380290468749003 1107 3 -0.14024534140916423 1107 4 -0.23030658105033375 1107 5 -1.1626030175809028 1107 6 -0.32515027424475207 1107 7 -0.3366593801068163 1107 8 -0.4126119717861769 1107 9 -0.3180015223273074 1107 10-11 -0.24080683841624761 1107 12-13 -0.4492530816456579 1107 14-15 -0.31104495165438806 1107 16-17 -0.3718076774723684 1107 18-19 -0.31292191820929105 1107 20-21 -0.17753973666844303 1107 22-23 -0.20184064887863684 1107 24-25 -0.24936642995303515 1107 26-27 -0.2551724717269934 1107 28-29 -0.1723782909173508 1107 30-31 -0.30339068991872153 1107 32-33 -0.3517937463327172 1107 34-35 -0.35131185759237127 1107 36-37 -0.3536052367590443 1107 38-39 -0.1875594513845229 1107 40-41 -0.5055444709724171 1107 42-43 -0.16787468511902404 1107 44-45 -0.27627192618709984 1107 46-47 -0.4715679305814291 1107 48-49 -0.3355460615558741 1107 50-51 -0.5767006483121406 1107 52-53 -0.4062466796555455 1107 54-55 -0.4186603822116055 1107 56-57 -0.3071143815798152 1107 58-59 -0.668419390723983 1107 60-61 -0.25281306194164443 1107 62-63 -0.47396580724355175 1107 64-65 -0.5335777659052781 1107 66-67 -0.5180479640293356 1107 68-69 -0.6503116465455676 1107 70-71 -0.4505187078998887 1107 72-73 -0.2659329291434034 1107 74-75 -0.4913019908286955 1107 76-77 -0.7885964809155652 1107 78-79 -0.9955512251391383 1107 80-81 -0.5944333822581811 1107 82-83 -0.7503545271372474 1107 84-85 -0.38344259060142605 1107 86-87 -0.7114028009271323 1107 88-89 -0.29614921672245487 1107 90-91 -0.21736422400938693 1107 92-93 -0.10930631039200023 1107 94-95 -0.15201759803075987 1107 96-97 -0.525721142692305 1107 98-99 -0.5296451149648291 1107 100 -0.35535809601914536 1108 1 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0.20603226247931872 2223 26-27 0.39437549939438554 2223 28-29 0.2631582656742353 2223 30-31 0.2934265474399993 2223 32-33 0.10548372233311198 2223 34-35 0.22142185135847114 2223 36-37 0.4244381500243719 2223 38-39 0.2708123631152475 2223 40-41 0.3281304580397304 2223 42-43 0.4584420250391119 2223 44-45 0.3859312695231196 2223 46-47 0.2144140433595041 2223 48-49 0.22311645362137256 2223 50-51 0.42932135292889484 2223 52-53 0.4639641799190173 2223 54-55 0.2718611641424431 2223 56-57 0.5024470605324538 2223 58-59 0.6444980911924532 2223 60-61 0.5076777476883194 2223 62-63 0.3922171847351521 2223 64-65 0.3523375417822052 2223 66-67 0.44773978246534796 2223 68-69 0.5998703426845253 2223 70-71 0.4323608059323263 2223 72-73 0.6305804533494026 2223 74-75 0.5249231152140368 2223 76-77 0.8939264924268855 2223 78-79 0.8399704555116045 2223 80-81 0.7273252983009613 2223 82-83 0.630844556339504 2223 84-85 0.47432550863211986 2223 86-87 0.7800490426909121 2223 88-89 0.6114773888977538 2223 90-91 0.6307803122848128 2223 92-93 0.6470240567062788 2223 94-95 0.7922437667403983 2223 96-97 0.7179863687625101 2223 98-99 0.8017843045497912 2223 100 0.9340285400141042 2224 1 1.3813859134143343 2224 2 -0.8007936154957012 2224 3 0.1004491415834039 2224 4 -0.3087288834526536 2224 5 -1.0429530688070763 2224 6 0.6057228321857586 2224 7 0.1437639982164569 2224 8 -0.03683526992935526 2224 9 0.07490356705294232 2224 10-11 0.08525032245449182 2224 12-13 0.06994833829588742 2224 14-15 -0.08002656162961586 2224 16-17 -0.0978573000434082 2224 18-19 0.006669094766870387 2224 20-21 -0.05185674958292452 2224 22-23 0.08645716878360332 2224 24-25 0.1006416223216533 2224 26-27 0.035491001441798176 2224 28-29 -0.06683387148635234 2224 30-31 0.02600525242014129 2224 32-33 0.03564230661356049 2224 34-35 0.2502406894963016 2224 36-37 0.022646035329032088 2224 38-39 0.0023995334120954226 2224 40-41 0.11603802372507488 2224 42-43 0.20455706756992242 2224 44-45 0.25114075803009683 2224 46-47 0.04848668782641852 2224 48-49 0.13831419892663632 2224 50-51 0.16670762257322735 2224 52-53 0.22268223075175797 2224 54-55 0.12524575910409652 2224 56-57 0.2965519732753634 2224 58-59 0.1935601667778215 2224 60-61 0.287831736802886 2224 62-63 0.29064461685434395 2224 64-65 0.17928257338672893 2224 66-67 0.07165608917735256 2224 68-69 0.06898674334214405 2224 70-71 0.39285769653674407 2224 72-73 0.2638392428806 2224 74-75 0.261848847121847 2224 76-77 0.2870602934459896 2224 78-79 0.07605782493230606 2224 80-81 0.09271552999943111 2224 82-83 0.05511622254665838 2224 84-85 0.03678198219969886 2224 86-87 0.1506665308711277 2224 88-89 0.06912731894912127 2224 90-91 0.15822162180496946 2224 92-93 0.031706837087980944 2224 94-95 0.3638844451911112 2224 96-97 0.38868146418879235 2224 98-99 0.2862781725562016 2224 100 0.4456869864395756 2225 1 1.2390727876849574 2225 2 1.580171927161384 2225 3 0.8107863444579202 2225 4 0.974300414502018 2225 5 3.0942006686783365 2225 6 0.01333907197447104 2225 7 0.22917644216953192 2225 8 0.44961519380137815 2225 9 0.3034091871008542 2225 10-11 0.28450098273334135 2225 12-13 0.14466119377159714 2225 14-15 0.17141990971735055 2225 16-17 0.17016984816616088 2225 18-19 0.13202687377306432 2225 20-21 0.2937362729586894 2225 22-23 0.3044321088978492 2225 24-25 0.2980926388439755 2225 26-27 0.1170308331474672 2225 28-29 0.14290140275969776 2225 30-31 0.33257118817347475 2225 32-33 0.17196685670261758 2225 34-35 0.33086841506598574 2225 36-37 0.23649346484810252 2225 38-39 0.14814303811082397 2225 40-41 0.2517176502836378 2225 42-43 0.3050300114922848 2225 44-45 0.16174821457453703 2225 46-47 0.27208474691369844 2225 48-49 0.30936634560067944 2225 50-51 0.1314671582282898 2225 52-53 0.40992502910567197 2225 54-55 0.20330300374509847 2225 56-57 0.5114282093787921 2225 58-59 0.2018735381923591 2225 60-61 0.42335473907547794 2225 62-63 0.31050519052917736 2225 64-65 0.22164176508257327 2225 66-67 0.3608582266382001 2225 68-69 0.2932880761841119 2225 70-71 0.36540238052421614 2225 72-73 0.5151536584570309 2225 74-75 0.4907752029967938 2225 76-77 0.37333877756859124 2225 78-79 0.3603493303680949 2225 80-81 0.34488370148768155 2225 82-83 0.1768685719109513 2225 84-85 0.089008503912126 2225 86-87 0.04858127812252633 2225 88-89 0.15333283458083713 2225 90-91 0.4890551086915238 2225 92-93 0.4258840989269359 2225 94-95 0.4698791629602894 2225 96-97 0.4338261113234836 2225 98-99 0.7322735045382629 2225 100 0.5456931285684377 2226 1 1.5500489243712536 2226 2 1.3836257087085713 2226 3 0.7093086214106261 2226 4 0.9346311018428164 2226 5 3.180596645782064 2226 6 -0.6004612399481317 2226 7 -0.10933365416434526 2226 8 0.07932643906389814 2226 9 0.20728723276994288 2226 10-11 -0.0813908155128118 2226 12-13 0.10998346578621465 2226 14-15 0.11324305207010354 2226 16-17 0.11595066286849232 2226 18-19 0.2562866748503936 2226 20-21 -0.04976952312294003 2226 22-23 0.22190121498034188 2226 24-25 0.18080480176798375 2226 26-27 0.10942926283524912 2226 28-29 0.19492005227473896 2226 30-31 0.1701841983912047 2226 32-33 0.1236805792396396 2226 34-35 0.24168653291346942 2226 36-37 0.23295758757606677 2226 38-39 0.02556835980672645 2226 40-41 0.23507834885858614 2226 42-43 0.31162647499775176 2226 44-45 0.13531092212857487 2226 46-47 0.2284752509691046 2226 48-49 0.14908833120615128 2226 50-51 0.3610831395293985 2226 52-53 0.28778551986938794 2226 54-55 0.4232259284104387 2226 56-57 0.35149586119415943 2226 58-59 0.46087526240832943 2226 60-61 0.5246339098057717 2226 62-63 0.31074489358544355 2226 64-65 0.44869817040089544 2226 66-67 0.3406264656746103 2226 68-69 0.5651308885902395 2226 70-71 0.5601523692077564 2226 72-73 0.5888103954503165 2226 74-75 0.5493234460659764 2226 76-77 0.7582771954872669 2226 78-79 0.6046520105157356 2226 80-81 0.3981276142645669 2226 82-83 0.37153934542138956 2226 84-85 0.30517745238728367 2226 86-87 0.6671458168844637 2226 88-89 0.24878094555614894 2226 90-91 0.6014172022765365 2226 92-93 0.3095550740760018 2226 94-95 0.3497292919678543 2226 96-97 0.3040078741388612 2226 98-99 0.5424790936316768 2226 100 0.5055752728511607 2227 1 1.3273073574105858 2227 2 1.6789765837464472 2227 3 0.6578382138239611 2227 4 0.008194475549913705 2227 5 3.324320695606403 2227 6 0.19884307013548153 2227 7 0.8987165305067251 2227 8 0.8569104009340265 2227 9 0.6429942650116303 2227 10-11 0.5945452334270769 2227 12-13 0.36260612259283675 2227 14-15 0.49471063431219875 2227 16-17 0.48815500489078545 2227 18-19 0.5041062458893819 2227 20-21 0.5114780098189442 2227 22-23 0.457023114267038 2227 24-25 0.4939655843474853 2227 26-27 0.28090822834967355 2227 28-29 0.23347986359085837 2227 30-31 0.3757628207695731 2227 32-33 0.27035281470140404 2227 34-35 0.39015736634917886 2227 36-37 0.47283855458096014 2227 38-39 0.5228249092830168 2227 40-41 0.5390659746683113 2227 42-43 0.6775137150865262 2227 44-45 0.5533114380610655 2227 46-47 0.5464677822321491 2227 48-49 0.40420773272447263 2227 50-51 0.40969442125309996 2227 52-53 0.45131666826958394 2227 54-55 0.596268046518027 2227 56-57 0.5710681285945967 2227 58-59 0.9005254929400692 2227 60-61 0.8217813354664543 2227 62-63 0.7635411657684941 2227 64-65 0.5945975308084002 2227 66-67 0.5333661490722292 2227 68-69 0.5905784395487998 2227 70-71 0.6611164668582177 2227 72-73 0.539990283910619 2227 74-75 0.38810989915297256 2227 76-77 0.6479106377396846 2227 78-79 0.7945274496725574 2227 80-81 0.4853488674072537 2227 82-83 0.6114510226933376 2227 84-85 0.5483290628336945 2227 86-87 0.7727471648604549 2227 88-89 0.6791525807345593 2227 90-91 0.6096751930880231 2227 92-93 0.6484223975329186 2227 94-95 0.8898276876635052 2227 96-97 0.9962019384584408 2227 98-99 0.8177331699077897 2227 100 0.8583952943073996 2228 1 1.7135996631117454 2228 2 1.5795622305464185 2228 3 -0.150095520725964 2228 4 -0.11455677016289378 2228 5 3.19772715389929 2228 6 0.16243428219786438 2228 7 0.20258147582478614 2228 8 0.4217764463621023 2228 9 0.27867989239789637 2228 10-11 0.3166258973758218 2228 12-13 0.22509237121769843 2228 14-15 0.2514730752621759 2228 16-17 0.08127608170435252 2228 18-19 -0.010636037162448986 2228 20-21 0.18238738016773937 2228 22-23 0.1408870888997953 2228 24-25 0.3636106370880512 2228 26-27 0.1675685855618596 2228 28-29 0.09416225535944278 2228 30-31 0.28603914251939244 2228 32-33 0.25630618945064754 2228 34-35 0.1973509533549347 2228 36-37 0.3658649895773962 2228 38-39 0.20806402142952152 2228 40-41 0.30792558121719793 2228 42-43 0.6141400983130865 2228 44-45 0.4781075941472608 2228 46-47 0.44827487470610805 2228 48-49 0.5196141263050009 2228 50-51 0.17079258989349455 2228 52-53 0.425039408190635 2228 54-55 0.2934344543352765 2228 56-57 0.49251542038259544 2228 58-59 0.46151284167494566 2228 60-61 0.6326513930604492 2228 62-63 0.34256303854400727 2228 64-65 0.3801933698040614 2228 66-67 0.5616252249798208 2228 68-69 0.6245362470942624 2228 70-71 0.5485857284902664 2228 72-73 0.47087749027831904 2228 74-75 0.42681193112087357 2228 76-77 0.6922466889918653 2228 78-79 0.5961921749686212 2228 80-81 0.4668047335821015 2228 82-83 0.45394519858151483 2228 84-85 0.2022352622771706 2228 86-87 0.470204778268851 2228 88-89 0.30887253802440284 2228 90-91 0.367384052209232 2228 92-93 0.25525440426299895 2228 94-95 0.828484420983898 2228 96-97 0.5255732579433285 2228 98-99 0.5408775431686408 2228 100 0.7278704771197937 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 7.0 13 22.0 14 70.0 15 265.0 16 936.0 17 2414.0 18 4003.0 19 5067.0 20 5902.0 21 6611.0 22 7742.0 23 8880.0 24 9939.0 25 11304.0 26 12749.0 27 13947.0 28 16003.0 29 18385.0 30 20757.0 31 23844.0 32 27956.0 33 33357.0 34 40819.0 35 52932.0 36 73975.0 37 123003.0 38 297365.0 39 536912.0 40 594.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.120952147280086 19.18799668977448 19.293707660510588 36.39734350243485 2 19.346853209784292 54.36942116999328 9.096300536334821 17.187425083887607 3 40.874638579099546 14.05359355638166 23.725216852540274 21.34655101197852 4 17.881114651560747 16.764029031687024 21.667109222871307 43.68774709388092 5 16.463312090635508 35.6041629786983 26.55971558387915 21.37280934678704 6 17.230999586947544 27.818787986074234 30.87006549831829 24.08014692865994 7 19.77134596093704 26.520254322298932 37.273706260695114 16.43469345606892 8 20.190667964831533 27.481117601935445 35.55393284947188 16.774281583761137 9 20.493818965008558 27.868796837198325 28.274104561279284 23.363279636513838 10-11 19.175444031391986 30.347369740957102 28.73664955449342 21.74053667315749 12-13 24.17894760134537 26.47046674927716 27.386853130347554 21.963732519029914 14-15 21.842804036112586 27.353735174367145 28.593003186404676 22.210457603115596 16-17 20.918193485572665 30.002581577860386 28.117292146102557 20.961932790464388 18-19 21.545996341535375 29.66542750929368 29.926425030978937 18.86215111819201 20-21 22.674809700832004 29.234709683129758 30.055282645896032 18.035197970142207 22-23 22.044351507641473 30.478513896264825 28.713968549005724 18.76316604708798 24-25 22.270313329792884 28.212884286304362 26.27865551425031 23.238146869652446 26-27 19.99605387384198 26.215333097303358 30.23330087921166 23.555312149643004 28-29 19.077405692629853 28.352067080065467 30.991522792131015 21.579004435173662 30-31 19.806746892499916 30.504971299353844 28.285489087558087 21.402792720588153 32-33 20.175436655455243 30.472096831297574 29.017414586652507 20.335051926594677 34-35 21.237977223107336 29.482282999940995 29.97595444621467 19.303785330737004 36-37 21.75425591550127 28.258688853484394 28.773307960110934 21.213747270903404 38-39 21.622968237887427 29.175316549763398 28.960269607129042 20.241445605220136 40-41 20.725271272315286 30.1335579583416 29.932302703835024 19.208868065508085 42-43 20.301336964266483 30.2159013629276 29.573013767943834 19.909747904862076 44-45 20.665936448929013 30.055540803682067 28.82191538325367 20.456607364135245 46-47 19.662630446905123 28.152374672130904 29.101756699741244 23.083238181222725 48-49 20.52841210833776 27.931713577624357 30.693965008556084 20.845909305481797 50-51 21.74053667315749 30.848269605239864 28.21926447158789 19.191929250014752 52-53 20.305102073180535 30.66982459064882 28.10595370951574 20.919119626654904 54-55 22.595514006556954 28.331776721347275 28.30377983752217 20.768929434573607 56-57 21.742371403500943 28.702134432078925 29.64451704827323 19.910977116146906 58-59 21.38202580809357 28.061479105845642 28.94036327556604 21.61613181049475 60-61 19.408581985656 30.489242737552445 29.84462203261127 20.257553244180286 62-63 20.432383227242784 31.5795627480907 27.134382263833878 20.853671760832633 64-65 19.692924661729947 30.305360431079727 29.109055926995847 20.892658980194483 66-67 20.17650616628312 30.40047648551366 29.55301823331563 19.86999911488759 68-69 20.905765032159085 30.01368236266006 29.448501209653628 19.63205139552723 70-71 19.414018705375582 31.138660234849823 29.410515135422198 20.036805924352393 72-73 20.022681005487698 30.946996518557857 28.519981412639407 20.51034106331504 74-75 19.832824393698 29.270851773175195 30.776096064200154 20.120227768926654 76-77 19.35029061190771 30.432635569717352 28.830323951141796 21.38674986723314 78-79 19.277674514663364 29.561168643417712 28.54483831946657 22.616318522452353 80-81 20.475888764809056 29.621916300696732 28.571655118391337 21.330539816102874 82-83 19.699393697999646 28.582197439074765 30.390592730276744 21.32781613264885 84-85 20.33667463267835 29.2656517377707 28.406318227414886 21.99135540213607 86-87 21.035839676638936 28.817526700891012 29.598011447453825 20.548622175016227 88-89 22.03786068330678 27.934995869475422 29.24673246002242 20.780410987195374 90-91 22.76439045258748 28.850829055290024 28.199902637634978 20.18487785448752 92-93 22.363471410869177 29.12237416651915 29.05351979701422 19.46063462559745 94-95 22.53695344308727 28.933587065557326 28.844596388741373 19.68486310261403 96-97 21.636351566648965 28.954092169705554 29.260156664896442 20.149399598749042 98-99 23.25750870360536 29.23006284298106 28.01495102378002 19.497477429633562 100 23.108367262642357 30.954077417832064 27.09439723844928 18.843158081076297 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2.0 1 8.5 2 10.5 3 9.0 4 14.0 5 22.0 6 27.5 7 25.0 8 25.0 9 35.0 10 54.0 11 56.0 12 51.0 13 63.5 14 72.5 15 90.5 16 114.0 17 157.5 18 256.0 19 429.5 20 650.5 21 972.5 22 1479.5 23 2320.0 24 3664.5 25 5501.0 26 7813.0 27 10474.0 28 13509.5 29 16748.0 30 20461.5 31 24746.5 32 29494.0 33 34811.5 34 40917.0 35 47866.5 36 54385.5 37 59051.5 38 61834.0 39 64086.0 40 65322.0 41 64965.5 42 63742.0 43 63105.0 44 63404.0 45 61725.5 46 57008.0 47 51377.5 48 47690.0 49 46310.0 50 44943.5 51 41681.5 52 36144.0 53 30282.5 54 25610.5 55 21382.0 56 16904.5 57 12390.5 58 8520.5 59 5855.0 60 4431.0 61 3694.0 62 3140.0 63 2473.5 64 1746.0 65 1377.0 66 1364.0 67 1283.0 68 959.5 69 888.0 70 1076.0 71 1013.5 72 654.0 73 305.0 74 140.0 75 82.0 76 53.5 77 41.0 78 32.0 79 27.0 80 23.5 81 18.0 82 15.0 83 11.0 84 7.0 85 10.0 86 11.0 87 8.0 88 8.5 89 7.5 90 6.0 91 7.5 92 7.0 93 3.5 94 6.5 95 9.0 96 7.0 97 6.5 98 18.0 99 49.5 100 51.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.316530949430579 2 0.0056794712928541925 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 5.163155720776538E-4 30-31 0.009478078715996931 32-33 0.0 34-35 0.0 36-37 0.0 38-39 7.007139906768159E-4 40-41 0.0014014279813536318 42-43 1.4751873487932966E-4 44-45 0.0 46-47 0.0026553372278279343 48-49 0.0 50-51 0.0 52-53 0.0012539092464743022 54-55 0.01858736059479554 56-57 0.011322062901988552 58-59 0.013092287720540509 60-61 1.1063905115949726E-4 62-63 0.011580220688027379 64-65 1.1063905115949726E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.4751873487932966E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1355760.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.85095638813557 #Duplication Level Percentage of deduplicated Percentage of total 1 88.26525968674028 69.5980014214662 2 7.5994331945139955 11.984451507903456 3 1.9579545559132412 4.63159767894799 4 0.8204464097168194 2.587719362855333 5 0.3946066003880173 1.5557553918832996 6 0.24948608488947693 1.18033298394401 7 0.170238843670758 0.9396446946494696 8 0.10477607333750724 0.6609354871404702 9 0.07345651779973231 0.5212905013305896 >10 0.3425344603162313 4.583117434634679 >50 0.015614502831546485 0.8361038259992466 >100 0.006002521288477797 0.8299234550723776 >500 1.9054859377869087E-4 0.09112625417294586 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 3.6879683719832416E-5 0.0 40-41 0.0 0.0 0.0 7.375936743966483E-5 0.0 42-43 0.0 0.0 0.0 7.375936743966483E-5 0.0 44-45 0.0 0.0 0.0 7.375936743966483E-5 0.0 46-47 0.0 0.0 0.0 7.375936743966483E-5 0.0 48-49 0.0 0.0 0.0 7.375936743966483E-5 0.0 50-51 0.0 0.0 0.0 7.375936743966483E-5 0.0 52-53 0.0 0.0 0.0 7.375936743966483E-5 0.0 54-55 0.0 0.0 0.0 7.375936743966483E-5 0.0 56-57 0.0 0.0 0.0 7.375936743966483E-5 0.0 58-59 7.375936743966483E-5 0.0 0.0 7.375936743966483E-5 0.0 60-61 1.1063905115949725E-4 0.0 0.0 7.375936743966483E-5 0.0 62-63 2.212781023189945E-4 0.0 0.0 7.375936743966483E-5 0.0 64-65 3.687968371983242E-4 0.0 0.0 7.375936743966483E-5 0.0 66-67 3.687968371983242E-4 0.0 0.0 7.375936743966483E-5 0.0 68-69 4.056765209181566E-4 0.0 0.0 7.375936743966483E-5 0.0 70-71 4.7943588835782145E-4 0.0 0.0 7.375936743966483E-5 0.0 72-73 6.269546232371511E-4 0.0 0.0 7.375936743966483E-5 0.0 74-75 6.638343069569836E-4 0.0 0.0 7.375936743966483E-5 0.0 76-77 7.744733581164808E-4 0.0 0.0 7.375936743966483E-5 0.0 78-79 0.0012539092464743022 0.0 0.0 7.375936743966483E-5 0.0 80-81 0.0014383076650734644 0.0 0.0 7.375936743966483E-5 0.0 82-83 0.001659585767392459 0.0 0.0 7.375936743966483E-5 0.0 84-85 0.0019915029208709507 0.0 0.0 7.375936743966483E-5 0.0 86-87 0.0019915029208709507 7.375936743966483E-5 0.0 7.375936743966483E-5 0.0 88 0.0019915029208709507 7.375936743966483E-5 0.0 7.375936743966483E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGCTGC 50 2.5465852E-11 84.68286 1 TCACCGG 5750 0.0 79.67256 8 CACCGGC 5535 0.0 77.679184 9 CTTCACC 6465 0.0 74.200905 6 CCTTCAC 7000 0.0 72.15079 5 GGCTGCG 30 7.6171214E-5 70.569046 1 GGCCCGA 435 0.0 70.13761 9 CAGCGTA 610 0.0 69.412186 1 GTGTGGC 2405 0.0 69.28514 7 GGGCCCG 455 0.0 69.11786 8 CGTAGTG 1205 0.0 68.16757 4 CGCGTAG 200 0.0 68.065475 2 CGGGGCA 610 0.0 66.94471 94 TTCACCG 7105 0.0 66.129745 7 GCCTTCA 2105 0.0 63.996563 4 AACGGGC 455 0.0 63.95981 5 CGGGCCC 480 0.0 63.562202 7 GAGCAGC 25 0.0032716189 63.512146 1 TGTGGCA 2645 0.0 62.643475 8 GCGTAGT 860 0.0 61.674667 3 >>END_MODULE