FastQCFastQC Report
Mon 6 Mar 2017
1NF_1A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_1A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1355760
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTGC502.5465852E-1184.682861
TCACCGG57500.079.672568
CACCGGC55350.077.6791849
CTTCACC64650.074.2009056
CCTTCAC70000.072.150795
GGCTGCG307.6171214E-570.5690461
GGCCCGA4350.070.137619
CAGCGTA6100.069.4121861
GTGTGGC24050.069.285147
GGGCCCG4550.069.117868
CGTAGTG12050.068.167574
CGCGTAG2000.068.0654752
CGGGGCA6100.066.9447194
TTCACCG71050.066.1297457
GCCTTCA21050.063.9965634
AACGGGC4550.063.959815
CGGGCCC4800.063.5622027
GAGCAGC250.003271618963.5121461
TGTGGCA26450.062.6434758
GCGTAGT8600.061.6746673
Produced by FastQC (version 0.11.5)