FastQCFastQC Report
Mon 6 Mar 2017
1NF_20A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_20A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2457026
Sequences flagged as poor quality0
Sequence length95
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA143030.5821265220636656No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA60670.2469245339691155No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA59190.24090099168669765No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG57270.233086666563561No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT54960.2236850566497872No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG50180.2042306430619782No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG46210.18807289788549245No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG46180.18795079905544346No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT41480.16882198234776513No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT38490.15665279895288042No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC37490.15258283795124675No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA36390.1481058808494497No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA34840.1417974412969175No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA34220.13927406547590462No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA32430.1319888352829803No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA30480.12405241132979464No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC28870.11749977411716442No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC26030.10594108487252474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTGC1350.088.999861
GCTGCTC158.5778726E-488.999863
GCTGCCT202.411284E-588.999863
GCTGCAG202.411284E-588.999863
GCTGCAC301.9388608E-888.999863
TGCTGCT355.5479177E-1088.999862
TGCTGCA301.9388608E-888.999862
GCAGCTT202.411284E-588.999863
GCAGCCT301.9388608E-888.999863
GCAGCCG301.9388608E-888.999863
GCAGCCA401.6370905E-1188.999863
AGCAGCT700.088.999862
GCTGCCA256.8161535E-788.9998553
TGCTGCC500.088.9998552
GCAGCTC256.8161535E-788.9998553
TGCAGCC256.8161535E-788.9998552
CAGCTGC4000.087.887361
CAGCAGC4850.087.164811
CTGCAGC1150.085.1302951
AGCAGCC1150.085.1302952
Produced by FastQC (version 0.11.5)