FastQCFastQC Report
Tue 7 Mar 2017
1NF_20A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_20A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2457026
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG99150.079.579578
CACCGGC95200.078.8384559
CTTCACC109700.073.723386
GTGTGGC43100.071.115217
CGGGCCC6150.070.979757
CCTTCAC121100.070.3104255
TTCACCG123750.065.542827
GCTGCTA651.0913936E-1164.9979862
CGCTGCA751.2732926E-1163.529491
CGTAGTG18050.062.9310154
TGTGTGG48150.061.2195246
ACCTTCA47300.061.1288454
CGGGGCA10100.060.4155394
TGTGGCA50100.060.0546888
GCTGCCT1150.059.846621
GGGCCCG7300.059.7980048
GCAGCCC950.059.2903754
ACGGGCC7300.059.1550146
GCCTTCA33000.058.7438934
CAGCGTA11350.058.305331
Produced by FastQC (version 0.11.5)