##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1NF_20A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2457026
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.83046903044575	12.0	12.0	12.0	2.0	32.0
2	14.621852597408411	12.0	12.0	12.0	12.0	22.0
3	13.7135003862393	12.0	12.0	12.0	12.0	22.0
4	13.743207031590224	12.0	12.0	12.0	12.0	22.0
5	22.434838703375544	22.0	22.0	22.0	22.0	27.0
6	26.83063834082342	27.0	22.0	32.0	22.0	32.0
7	34.945273269391535	37.0	37.0	37.0	27.0	41.0
8	37.558486153585676	41.0	37.0	41.0	27.0	41.0
9	38.52768143275651	41.0	41.0	41.0	32.0	41.0
10-11	38.889465353642976	41.0	41.0	41.0	37.0	41.0
12-13	39.06283368592762	41.0	41.0	41.0	37.0	41.0
14-15	39.01518604198735	41.0	41.0	41.0	37.0	41.0
16-17	38.92110360248528	41.0	41.0	41.0	37.0	41.0
18-19	38.85028628105685	41.0	41.0	41.0	37.0	41.0
20-21	38.87615922664229	41.0	41.0	41.0	37.0	41.0
22-23	38.86115857137857	41.0	41.0	41.0	37.0	41.0
24-25	38.880406637943594	41.0	41.0	41.0	37.0	41.0
26-27	38.840339092870806	41.0	41.0	41.0	37.0	41.0
28-29	38.78492575984137	41.0	41.0	41.0	34.5	41.0
30-31	38.66563784835814	41.0	41.0	41.0	32.0	41.0
32-33	38.720026975701515	41.0	41.0	41.0	32.0	41.0
34-35	38.691237496062314	41.0	41.0	41.0	32.0	41.0
36-37	38.61778548537948	41.0	41.0	41.0	32.0	41.0
38-39	38.52108138049822	41.0	41.0	41.0	32.0	41.0
40-41	38.568770741538756	41.0	41.0	41.0	32.0	41.0
42-43	38.308666656356095	41.0	41.0	41.0	32.0	41.0
44-45	38.421076944240724	41.0	41.0	41.0	32.0	41.0
46-47	38.428068526747374	41.0	41.0	41.0	32.0	41.0
48-49	38.37957127844801	41.0	41.0	41.0	32.0	41.0
50-51	38.267316463073655	41.0	41.0	41.0	32.0	41.0
52-53	38.23936559889883	41.0	41.0	41.0	32.0	41.0
54-55	38.11035353309245	41.0	39.0	41.0	32.0	41.0
56-57	37.891739851348746	41.0	37.0	41.0	29.5	41.0
58-59	37.89354610004126	41.0	37.0	41.0	29.5	41.0
60-61	37.899052146782324	41.0	37.0	41.0	29.5	41.0
62-63	37.905833312305205	41.0	37.0	41.0	29.5	41.0
64-65	37.89565311885181	41.0	37.0	41.0	29.5	41.0
66-67	37.75317436608323	41.0	37.0	41.0	27.0	41.0
68-69	37.766784926166835	41.0	37.0	41.0	27.0	41.0
70-71	37.740844622726826	41.0	37.0	41.0	27.0	41.0
72-73	37.60077833934196	41.0	37.0	41.0	27.0	41.0
74-75	37.506656828214275	41.0	37.0	41.0	27.0	41.0
76-77	36.45123413427453	39.0	34.5	41.0	24.5	41.0
78-79	36.9680042050837	41.0	37.0	41.0	27.0	41.0
80-81	37.63482865057187	41.0	37.0	41.0	27.0	41.0
82-83	37.811482458875076	41.0	37.0	41.0	27.0	41.0
84-85	37.73153356944533	41.0	37.0	41.0	27.0	41.0
86-87	37.60447793389244	41.0	37.0	41.0	27.0	41.0
88-89	37.51748495945912	41.0	37.0	41.0	27.0	41.0
90-91	37.36307084255519	41.0	37.0	41.0	27.0	41.0
92-93	37.26487753894342	41.0	37.0	41.0	27.0	41.0
94-95	37.10723309399249	41.0	37.0	41.0	27.0	41.0
96-97	37.04303739561568	41.0	37.0	41.0	27.0	41.0
98-99	36.92322079619833	41.0	37.0	41.0	27.0	41.0
100	36.765729381781064	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.4762125933302226
1101	2	-0.6143509857990939
1101	3	0.07173216907209046
1101	4	-0.060975362624994034
1101	5	-1.1858050112996068
1101	6	-0.8445298400306243
1101	7	-0.41652977148034154
1101	8	-0.06699788390719874
1101	9	-0.21690171581496998
1101	10-11	-0.05406895791366395
1101	12-13	-0.16710615847643595
1101	14-15	-0.04838869398647461
1101	16-17	-0.1845946848829385
1101	18-19	-0.1427293874146045
1101	20-21	0.05399550817189436
1101	22-23	-0.04203080268153769
1101	24-25	0.00458627210667828
1101	26-27	0.05330041248377171
1101	28-29	-0.014921120050317427
1101	30-31	-0.19221638817870002
1101	32-33	-0.256534745994756
1101	34-35	-0.14939412050802758
1101	36-37	0.12118341037466962
1101	38-39	-0.09449245490677072
1101	40-41	-0.23845654831764307
1101	42-43	0.033227146874807545
1101	44-45	-0.011821145956730561
1101	46-47	0.1800260094245303
1101	48-49	0.11987780396375314
1101	50-51	0.047761341170911464
1101	52-53	-0.10447562205688854
1101	54-55	-0.010158601001137413
1101	56-57	-0.10643175880167632
1101	58-59	-0.12300955303398808
1101	60-61	-0.07851177328931414
1101	62-63	-0.14517931460243716
1101	64-65	-0.011729268419863104
1101	66-67	-0.08942666419044798
1101	68-69	-0.11595363654456747
1101	70-71	-0.0874409178826312
1101	72-73	0.027992212347285772
1101	74-75	-0.11930644203389562
1101	76-77	-0.48214697222650216
1101	78-79	-0.1816196722145591
1101	80-81	-0.14510360191606964
1101	82-83	-0.04072716714463809
1101	84-85	-0.16115723932399106
1101	86-87	-4.586166568998351E-5
1101	88-89	-0.2591402505268121
1101	90-91	-0.43097515335384884
1101	92-93	-0.09087749935056877
1101	94-95	-0.06919063667393033
1101	96-97	-0.25041899269135115
1101	98-99	-0.2065707931091083
1101	100	-0.5685679460287432
1102	1	1.3903984286173827
1102	2	0.7972072171031304
1102	3	-0.0899179164738193
1102	4	-0.18279696243911658
1102	5	-0.7272937213517814
1102	6	-0.34958739869822253
1102	7	-0.24202329745039464
1102	8	-0.39269629761144387
1102	9	-0.3283779636235167
1102	10-11	-0.2999200869084504
1102	12-13	-0.2986928766818693
1102	14-15	-0.049316814299935174
1102	16-17	-0.2611263791144012
1102	18-19	-0.20252935784318282
1102	20-21	-0.3354124296436183
1102	22-23	-0.24622825531444903
1102	24-25	-0.19725497158299277
1102	26-27	-0.26927335766176697
1102	28-29	-0.24426019868919013
1102	30-31	-0.13804111411494802
1102	32-33	-0.14306432798153423
1102	34-35	-0.1577957849568392
1102	36-37	-0.3099591027740516
1102	38-39	-0.2969766658777644
1102	40-41	-0.20654538932899413
1102	42-43	-0.15021412216179897
1102	44-45	-0.19235512141244726
1102	46-47	-0.35907375189182034
1102	48-49	-0.305736542402137
1102	50-51	-0.26854011839016323
1102	52-53	-0.22844284001941162
1102	54-55	-0.2103258907799841
1102	56-57	-0.34699784037656656
1102	58-59	-0.21434639269349276
1102	60-61	-0.18901934568650347
1102	62-63	-0.31260829037498894
1102	64-65	-0.22910607174875253
1102	66-67	-0.16458983509216552
1102	68-69	-0.5413216951804785
1102	70-71	-0.5687585361155243
1102	72-73	-0.3824114376115304
1102	74-75	-0.5247110425028083
1102	76-77	-0.321401349853609
1102	78-79	-0.19230108234910404
1102	80-81	-0.3686230240059274
1102	82-83	-0.317052072621685
1102	84-85	-0.3738733747611249
1102	86-87	-0.17710392727201452
1102	88-89	-0.18670293138776373
1102	90-91	-0.28476684378326667
1102	92-93	-0.3181446771804488
1102	94-95	-0.11948930807202629
1102	96-97	-0.2598826937664853
1102	98-99	-0.44754094709175263
1102	100	-0.5272216012681596
1103	1	1.4594834349116912
1103	2	0.56255760028297
1103	3	0.26911165573404716
1103	4	1.018139454001176
1103	5	2.8966401111091855
1103	6	-0.2925892317775727
1103	7	-0.4961362416408548
1103	8	-0.19677500959713257
1103	9	-0.2599718592484166
1103	10-11	-0.28377595828369095
1103	12-13	-0.3272849392274182
1103	14-15	-0.27948267485339073
1103	16-17	-0.34793880871752947
1103	18-19	-0.3215838864325917
1103	20-21	-0.1629103867539854
1103	22-23	-0.049393961270851605
1103	24-25	-0.20667854542517006
1103	26-27	-0.18608181191208217
1103	28-29	-0.12664938572953588
1103	30-31	-0.11377953589988721
1103	32-33	-0.19750656389663845
1103	34-35	-0.41645255741206455
1103	36-37	-0.2474137727093435
1103	38-39	-0.10300970547669408
1103	40-41	-0.31248986690137315
1103	42-43	-0.09894355523231724
1103	44-45	-0.366761358299307
1103	46-47	-0.5020197091788603
1103	48-49	-0.27999436611732165
1103	50-51	-0.257343316340652
1103	52-53	-0.14177595249981323
1103	54-55	-0.28458084340703493
1103	56-57	-0.1255240887477811
1103	58-59	-0.373410638812004
1103	60-61	-0.309068816743725
1103	62-63	-0.3580814677861568
1103	64-65	-0.2914846578482724
1103	66-67	-0.3213435178148387
1103	68-69	-0.4407761884334249
1103	70-71	-0.43111061986296306
1103	72-73	-0.3056270563310264
1103	74-75	-0.5028033148586459
1103	76-77	-0.49706865699378255
1103	78-79	-0.428374758887891
1103	80-81	-0.4307381325551418
1103	82-83	-0.44160981462129456
1103	84-85	-0.5226209177722296
1103	86-87	-0.3092428879381046
1103	88-89	-0.3956452666866852
1103	90-91	-0.4336287886142074
1103	92-93	-0.23140954610423137
1103	94-95	-0.21211237836951824
1103	96-97	-0.2119328715531026
1103	98-99	-0.31306359556013064
1103	100	-0.3652122812743457
1104	1	1.4709908079825826
1104	2	-0.5273958973795381
1104	3	0.06206732273965976
1104	4	0.18554742705518557
1104	5	-1.0089574481257273
1104	6	-0.5015287018462509
1104	7	-0.3035969877552418
1104	8	-0.2725705495620545
1104	9	-0.0653797059157668
1104	10-11	-0.14027395620406224
1104	12-13	-0.07661511201036575
1104	14-15	-0.20382556363710336
1104	16-17	0.057237056556061816
1104	18-19	-0.06821983583727587
1104	20-21	-0.04526384782622728
1104	22-23	0.12422452423688668
1104	24-25	-0.11248347158840488
1104	26-27	-0.018895788862870688
1104	28-29	0.01634397631919171
1104	30-31	0.04717288482452631
1104	32-33	0.04938083309933461
1104	34-35	-0.10101177508745707
1104	36-37	-0.04248564297122215
1104	38-39	-0.06817051482860847
1104	40-41	-0.049560312440874554
1104	42-43	-0.05661904819554309
1104	44-45	0.03948315923727819
1104	46-47	-0.15174205624105497
1104	48-49	-0.3040738977972879
1104	50-51	-0.03099220535327163
1104	52-53	0.08775095482296535
1104	54-55	-0.022383094238598744
1104	56-57	0.01784335130804493
1104	58-59	0.11611926467012523
1104	60-61	-0.05491705906319311
1104	62-63	-0.011503828591791887
1104	64-65	-0.0747781528681557
1104	66-67	-0.359063197499772
1104	68-69	-0.4423459682333899
1104	70-71	-0.37882995927611063
1104	72-73	-0.28390147624496365
1104	74-75	-0.37535893714282764
1104	76-77	-0.3283279896103366
1104	78-79	-0.37923973418283907
1104	80-81	-0.40619139003880633
1104	82-83	-0.2768094503095213
1104	84-85	-0.22107780732574867
1104	86-87	-0.2246026016148619
1104	88-89	-0.1551452571855947
1104	90-91	-0.2191285263397873
1104	92-93	-0.2714140182476825
1104	94-95	-0.0785512838963669
1104	96-97	-0.08804820070859876
1104	98-99	-0.35984466867128617
1104	100	-8.225189475581374E-4
1105	1	1.497284015100604
1105	2	0.28634673078738615
1105	3	0.04175196544515636
1105	4	-0.2416721187851767
1105	5	-1.1247434256192328
1105	6	0.43161673095771036
1105	7	0.3487523910022574
1105	8	0.12935920258995282
1105	9	0.20349840109209083
1105	10-11	0.03280574040070405
1105	12-13	0.09224371571600187
1105	14-15	0.018016504900415953
1105	16-17	-0.0374130806895181
1105	18-19	0.07720119694683802
1105	20-21	-0.09807847445929241
1105	22-23	0.04546134210769992
1105	24-25	0.12628470321720187
1105	26-27	-0.04557299091100475
1105	28-29	-0.07502062352648409
1105	30-31	0.07750360615809626
1105	32-33	0.14187429956505326
1105	34-35	0.10438797201248207
1105	36-37	0.03292126864058531
1105	38-39	0.11893289720124045
1105	40-41	0.061056739097381296
1105	42-43	0.08566745762238526
1105	44-45	0.24442679968313996
1105	46-47	-0.1279688357357074
1105	48-49	-0.02301686293805716
1105	50-51	-0.03475728571754644
1105	52-53	0.02382677478629347
1105	54-55	0.02412302910723696
1105	56-57	0.24738514917209642
1105	58-59	0.0477271270538111
1105	60-61	-0.0954349400135257
1105	62-63	-0.12606931515716724
1105	64-65	-0.10997694580273532
1105	66-67	-0.07223648239665437
1105	68-69	-0.06080612417286346
1105	70-71	-0.12887089328413026
1105	72-73	-0.11792096677139341
1105	74-75	-0.13917670996799103
1105	76-77	-0.24097502859689968
1105	78-79	-0.21341723422207082
1105	80-81	-0.2228552853529422
1105	82-83	-0.028164832580138466
1105	84-85	-0.07202181342046998
1105	86-87	-0.09402067696991878
1105	88-89	-0.07501990580000495
1105	90-91	-0.16557666337581622
1105	92-93	-0.05591322141313526
1105	94-95	-0.09530326156039592
1105	96-97	-0.11379441919100941
1105	98-99	-0.2575725342957469
1105	100	-0.19640648180916997
1106	1	1.200136332490498
1106	2	-0.8175382317993627
1106	3	-0.06330697243546268
1106	4	-0.23165022499543753
1106	5	-1.090021276227155
1106	6	-0.6523932934530876
1106	7	-0.029688833254503777
1106	8	0.022188493535722387
1106	9	0.006543857029534195
1106	10-11	0.02896656641618023
1106	12-13	0.006812105177949945
1106	14-15	-0.04521927472866594
1106	16-17	-0.05622510662647073
1106	18-19	-0.03709757882157305
1106	20-21	-0.10815700096822667
1106	22-23	-0.08464037445099848
1106	24-25	-0.15560790772315158
1106	26-27	-0.17811463378843229
1106	28-29	-0.19289460188271335
1106	30-31	-0.006459119444635064
1106	32-33	0.009495737943836957
1106	34-35	-0.05225655615515734
1106	36-37	-0.25193365301538506
1106	38-39	-0.059379623957369176
1106	40-41	-0.27065095552725893
1106	42-43	-0.05938288466996511
1106	44-45	-0.05386947837260436
1106	46-47	-0.19882695986706977
1106	48-49	-0.26440176784333147
1106	50-51	-0.1616743557000646
1106	52-53	-0.1808177584153725
1106	54-55	-0.2629943696806549
1106	56-57	-0.062140767847964185
1106	58-59	-0.13815037176004807
1106	60-61	-0.04096307368016738
1106	62-63	-0.21375427866681918
1106	64-65	-0.4092561161627586
1106	66-67	-0.33728432026430966
1106	68-69	-0.13523483637420242
1106	70-71	-0.3817358796230508
1106	72-73	-0.16844542770132165
1106	74-75	-0.2941664091056211
1106	76-77	-0.1827161113557665
1106	78-79	-0.14958744029386395
1106	80-81	-0.1629664994283999
1106	82-83	-0.2685348581823206
1106	84-85	-0.3999785342769542
1106	86-87	-0.27111360203308266
1106	88-89	-0.38771589171481935
1106	90-91	-0.4738165981684901
1106	92-93	-0.25958797173625214
1106	94-95	-0.20805061544903225
1106	96-97	-0.18775360420003295
1106	98-99	-0.42548110056085875
1106	100	-0.44223653079437497
1107	1	1.226773427282069
1107	2	-0.6095697642345321
1107	3	-0.2959994667189836
1107	4	-0.18392076199258867
1107	5	-1.2105392801002175
1107	6	-0.22598141222227497
1107	7	-0.49848161965611837
1107	8	-0.5516564625493245
1107	9	-0.3926627164837555
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2221	3	-0.2853336768887438
2221	4	-0.15192235979565005
2221	5	-0.6635096323439171
2221	6	-0.009381609840936278
2221	7	0.32624004536372553
2221	8	0.17514668381736698
2221	9	0.1875568699050092
2221	10-11	0.21272128836343285
2221	12-13	0.1922519953274957
2221	14-15	0.0590052954541207
2221	16-17	0.1817406528414267
2221	18-19	0.2150938226821708
2221	20-21	0.2528661800267784
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2221	24-25	0.2180451371672234
2221	26-27	0.11447517839311416
2221	28-29	0.12384252843143884
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2221	34-35	0.31943714962573466
2221	36-37	0.24096966583896773
2221	38-39	0.17553818270847898
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2221	44-45	0.2827732874883395
2221	46-47	0.2168568989363635
2221	48-49	0.1365279715680856
2221	50-51	0.3028203048991145
2221	52-53	0.2468114681619511
2221	54-55	0.2740306007709634
2221	56-57	0.3745689991750467
2221	58-59	0.33572931418286345
2221	60-61	0.2742224562208051
2221	62-63	0.18449686271184618
2221	64-65	0.19910812433623448
2221	66-67	0.17121094289009875
2221	68-69	0.21532670702799095
2221	70-71	0.3529672460494382
2221	72-73	0.26747834823420646
2221	74-75	0.33146345975584524
2221	76-77	0.4854852571398709
2221	78-79	0.38845703208957616
2221	80-81	0.3833315518682596
2221	82-83	0.15546444904470036
2221	84-85	0.19493601958387075
2221	86-87	0.273446516094225
2221	88-89	0.4486429779312928
2221	90-91	0.4181562363572695
2221	92-93	0.15874841299856968
2221	94-95	0.23922911254032186
2221	96-97	0.35425755217629273
2221	98-99	0.2644696769671455
2221	100	0.3076972543450225
2222	1	1.6894386648669215
2222	2	1.8613637324224346
2222	3	0.6475445103573776
2222	4	0.7530465222415774
2222	5	3.1576242768552554
2222	6	0.2439283952162441
2222	7	0.45171925383368716
2222	8	0.4688625274689784
2222	9	0.2942619140329725
2222	10-11	0.3683908343592819
2222	12-13	0.3795468533186508
2222	14-15	0.4448607947281715
2222	16-17	0.30600909523037245
2222	18-19	0.40916499431629205
2222	20-21	0.32712197946886334
2222	22-23	0.3389571056516445
2222	24-25	0.25396160807212453
2222	26-27	0.3428006812561293
2222	28-29	0.3688416311900511
2222	30-31	0.2994334415988078
2222	32-33	0.3399035568468207
2222	34-35	0.2761787971240537
2222	36-37	0.2730224929650191
2222	38-39	0.5072215095164196
2222	40-41	0.23320465022172243
2222	42-43	0.4552417656196681
2222	44-45	0.33275799360099256
2222	46-47	0.34905787285110534
2222	48-49	0.40287285484513546
2222	50-51	0.41151630728087696
2222	52-53	0.48288342692146813
2222	54-55	0.4661372215967461
2222	56-57	0.48070980081944015
2222	58-59	0.41676999184481645
2222	60-61	0.48344334481605955
2222	62-63	0.4916811153097669
2222	64-65	0.43516224022719285
2222	66-67	0.5752192668431846
2222	68-69	0.8192515263562044
2222	70-71	0.6191742804219444
2222	72-73	0.6879355538982566
2222	74-75	0.5080907293687247
2222	76-77	0.689797827491752
2222	78-79	0.7012199849925622
2222	80-81	0.672144786994437
2222	82-83	0.3890809232123118
2222	84-85	0.5092685331648426
2222	86-87	0.317275126675149
2222	88-89	0.5337236888727404
2222	90-91	0.6308245710772482
2222	92-93	0.5322724509110941
2222	94-95	0.7509552418409626
2222	96-97	0.5188121330014326
2222	98-99	0.5555150039708465
2222	100	0.6470461833650702
2223	1	1.3144922899205458
2223	2	-0.9865556154969113
2223	3	-0.07556635561063096
2223	4	-0.0927278235327691
2223	5	-0.9544674066649996
2223	6	0.2844352285802181
2223	7	0.4897470775757924
2223	8	0.45797204514992274
2223	9	0.3631409971977746
2223	10-11	0.35320268702827917
2223	12-13	0.2917764298339449
2223	14-15	0.31678731308326036
2223	16-17	0.30062513984641726
2223	18-19	0.22283938299067785
2223	20-21	0.36208704657678226
2223	22-23	0.291063804186912
2223	24-25	0.3035663148196903
2223	26-27	0.31752949169922573
2223	28-29	0.13043077135061765
2223	30-31	0.3672553451349998
2223	32-33	0.30805027499353344
2223	34-35	0.36809673547056576
2223	36-37	0.3244856908567826
2223	38-39	0.3709020267683627
2223	40-41	0.396037891626392
2223	42-43	0.5146476000255049
2223	44-45	0.4606031857318982
2223	46-47	0.3583104928180134
2223	48-49	0.22101497655868485
2223	50-51	0.34775152208752047
2223	52-53	0.4192902418997164
2223	54-55	0.4789827201565373
2223	56-57	0.47639834650799173
2223	58-59	0.5149559328879008
2223	60-61	0.5823739080323307
2223	62-63	0.49588531951396675
2223	64-65	0.5084845421209252
2223	66-67	0.524206519401865
2223	68-69	0.5875437982198974
2223	70-71	0.44927938552704916
2223	72-73	0.5212581622208603
2223	74-75	0.563560484838483
2223	76-77	0.7571907305989427
2223	78-79	0.717465311952175
2223	80-81	0.7182730581227119
2223	82-83	0.49344447043299766
2223	84-85	0.4506594138414357
2223	86-87	0.5547544427258941
2223	88-89	0.536846199138111
2223	90-91	0.5177232044044544
2223	92-93	0.4752368438754928
2223	94-95	0.599066107808973
2223	96-97	0.6472962293426718
2223	98-99	0.8647783489484766
2223	100	1.1452045458091504
2224	1	1.2969226841156463
2224	2	-0.8886183116772557
2224	3	-0.15416078714623893
2224	4	-0.25262754225601647
2224	5	-1.0111304045044704
2224	6	0.5410513469610443
2224	7	0.4798371676658846
2224	8	0.2421390175521907
2224	9	0.4652892993460753
2224	10-11	0.4157938981489053
2224	12-13	0.3598107537506223
2224	14-15	0.3148723891095173
2224	16-17	0.3878413152978908
2224	18-19	0.28938477453606737
2224	20-21	0.20338479228629325
2224	22-23	0.4007484888715993
2224	24-25	0.3744928975109758
2224	26-27	0.2639014292476318
2224	28-29	0.3311686132649285
2224	30-31	0.2873014092987134
2224	32-33	0.18187726940876559
2224	34-35	0.38395524103495404
2224	36-37	0.2787160524400889
2224	38-39	0.37145540820409906
2224	40-41	0.43048817901784986
2224	42-43	0.5223429650738112
2224	44-45	0.399849445566403
2224	46-47	0.35162813172388496
2224	48-49	0.3669415592499732
2224	50-51	0.3294563037923126
2224	52-53	0.43715539211780197
2224	54-55	0.5067129390632275
2224	56-57	0.5300957090288847
2224	58-59	0.43599665686979705
2224	60-61	0.5581983750332711
2224	62-63	0.48675401626501724
2224	64-65	0.4493817741946273
2224	66-67	0.4665845308975207
2224	68-69	0.4173071588656114
2224	70-71	0.5292547228553275
2224	72-73	0.4796363367755063
2224	74-75	0.42508060224095345
2224	76-77	0.7220922837357833
2224	78-79	0.4879567309730035
2224	80-81	0.42778653087147944
2224	82-83	0.3707833477718694
2224	84-85	0.4741083152315113
2224	86-87	0.5233390907223026
2224	88-89	0.5207420859751721
2224	90-91	0.5607514136091325
2224	92-93	0.38165117676041405
2224	94-95	0.6300361049554439
2224	96-97	0.5207329013087545
2224	98-99	0.5838807092273086
2224	100	0.9162636139270361
2225	1	0.9139455859531402
2225	2	1.7495756734997716
2225	3	0.8415595664020081
2225	4	0.8372912670816124
2225	5	3.2486415218992803
2225	6	0.018119356955597965
2225	7	0.3201747953868974
2225	8	0.49883036237429934
2225	9	0.3404319709791963
2225	10-11	0.2675907868117591
2225	12-13	0.40665730359976493
2225	14-15	0.32916809679900894
2225	16-17	0.20534836596013406
2225	18-19	0.2074006990824202
2225	20-21	0.23701780808018214
2225	22-23	0.30068185182139473
2225	24-25	0.2420745253847656
2225	26-27	0.22441792166628716
2225	28-29	0.1836049361862635
2225	30-31	0.1522684124003959
2225	32-33	0.21965012450986876
2225	34-35	0.08213644154714217
2225	36-37	0.2518592105337305
2225	38-39	0.21606363662344563
2225	40-41	0.28414587966773297
2225	42-43	0.4869617066688008
2225	44-45	0.28109357839022664
2225	46-47	0.3014605842688596
2225	48-49	0.3369221209309572
2225	50-51	0.43002186439785106
2225	52-53	0.39259359856602316
2225	54-55	0.4385874603921067
2225	56-57	0.503106196072352
2225	58-59	0.45436064190611347
2225	60-61	0.30553098336621787
2225	62-63	0.2984879665857463
2225	64-65	0.3674197100743086
2225	66-67	0.5070000526063225
2225	68-69	0.5543768574008041
2225	70-71	0.5532727058131286
2225	72-73	0.4485576407309111
2225	74-75	0.3541316424020877
2225	76-77	0.5666129693636961
2225	78-79	0.4839972036839768
2225	80-81	0.4824104184950784
2225	82-83	0.3763637735655365
2225	84-85	0.2022594034356544
2225	86-87	0.42109232658931717
2225	88-89	0.28417151201206536
2225	90-91	0.4961166911830972
2225	92-93	0.5237732301062294
2225	94-95	0.721444850389851
2225	96-97	0.5506437905867188
2225	98-99	0.6620150125334305
2225	100	0.5721145077479832
2226	1	1.6273158391658207
2226	2	1.212294306995183
2226	3	0.7812516673415768
2226	4	1.0406371708545894
2226	5	3.086996238632512
2226	6	-0.4466224150972309
2226	7	-0.009619701343702047
2226	8	0.11978223507512809
2226	9	0.30762683401587765
2226	10-11	0.11164498372936293
2226	12-13	0.247838493723485
2226	14-15	0.22161490425270358
2226	16-17	0.24335378816612518
2226	18-19	0.24663646852897614
2226	20-21	0.1880008997270579
2226	22-23	0.2936056317287381
2226	24-25	0.28420002931922284
2226	26-27	0.29187807579221925
2226	28-29	0.29769632535738566
2226	30-31	0.3665137774605256
2226	32-33	0.09214758544867152
2226	34-35	0.11071981267830466
2226	36-37	0.3067368945936195
2226	38-39	0.11430532280744643
2226	40-41	0.3818625798472368
2226	42-43	0.420498624486747
2226	44-45	0.3806530621715538
2226	46-47	0.37539070630397475
2226	48-49	0.18605079055615903
2226	50-51	0.18782269778170502
2226	52-53	0.2539765713144817
2226	54-55	0.39056326464442037
2226	56-57	0.36304663331602427
2226	58-59	0.3470417384561344
2226	60-61	0.5124327893691714
2226	62-63	0.5093848528218103
2226	64-65	0.3278639854620309
2226	66-67	0.26574037411463536
2226	68-69	0.4513061799535265
2226	70-71	0.5360260477529408
2226	72-73	0.4803844610436414
2226	74-75	0.40670627782453295
2226	76-77	0.5434089204912524
2226	78-79	0.4201944331317762
2226	80-81	0.4271830749400749
2226	82-83	0.3222737104443425
2226	84-85	0.24882646496376282
2226	86-87	0.450034684172266
2226	88-89	0.31739788272493996
2226	90-91	0.1422863229771565
2226	92-93	0.3542005405708153
2226	94-95	0.44143795249711104
2226	96-97	0.36989122278430386
2226	98-99	0.4795099906769309
2226	100	0.40682826446161613
2227	1	1.3605474608617705
2227	2	1.931743266794678
2227	3	0.4998194221452472
2227	4	-0.04568305770951575
2227	5	3.2508263100234274
2227	6	0.17181727790392642
2227	7	0.5342583162529451
2227	8	0.40602283066652234
2227	9	0.3156223755679406
2227	10-11	0.30536727185011614
2227	12-13	0.3306211086056976
2227	14-15	0.33023163707909475
2227	16-17	0.39034443982096434
2227	18-19	0.3151228013224099
2227	20-21	0.3267164207093529
2227	22-23	0.39550692095910733
2227	24-25	0.452173490819753
2227	26-27	0.3707898167462389
2227	28-29	0.39239049771326506
2227	30-31	0.3329730683276537
2227	32-33	0.24989617741373138
2227	34-35	0.3158738425685499
2227	36-37	0.38410631877732015
2227	38-39	0.24131211324500157
2227	40-41	0.43457542642645564
2227	42-43	0.6004805843315992
2227	44-45	0.4745999993185066
2227	46-47	0.44769205760978537
2227	48-49	0.35893391190241886
2227	50-51	0.3326082566707811
2227	52-53	0.5060510227352424
2227	54-55	0.4229379789705021
2227	56-57	0.409873754459241
2227	58-59	0.5429413476554501
2227	60-61	0.6452213954468178
2227	62-63	0.4751157349795747
2227	64-65	0.4506429265764851
2227	66-67	0.3247553134100869
2227	68-69	0.5403789259638714
2227	70-71	0.6528064084478729
2227	72-73	0.5751054689624198
2227	74-75	0.6402216491933572
2227	76-77	0.81166854478505
2227	78-79	0.5951515898435602
2227	80-81	0.51620579030795
2227	82-83	0.25510772895131595
2227	84-85	0.4860912221310443
2227	86-87	0.4950859775423879
2227	88-89	0.4603037040450175
2227	90-91	0.3847879674354928
2227	92-93	0.5532481277208632
2227	94-95	0.6932108678835434
2227	96-97	0.5165610958500153
2227	98-99	0.6328103280561237
2227	100	0.6695916950367717
2228	1	1.1151833919815122
2228	2	1.5291175919571192
2228	3	-0.25414219172071384
2228	4	-0.10192194916525033
2228	5	3.2575528327524985
2228	6	0.3923643906189689
2228	7	0.4519832863873461
2228	8	0.5767058474453677
2228	9	0.4924758114640895
2228	10-11	0.2543349837085245
2228	12-13	0.2975150451616102
2228	14-15	0.22080742908967466
2228	16-17	0.2696859815100012
2228	18-19	0.1962165272582297
2228	20-21	0.21176926303982668
2228	22-23	0.3037165254012777
2228	24-25	0.3376956496339574
2228	26-27	0.2241258140489677
2228	28-29	0.3459065210729406
2228	30-31	0.25054548458952297
2228	32-33	0.24114295671635233
2228	34-35	0.2277505673846676
2228	36-37	0.3763337718144584
2228	38-39	0.37280564271307526
2228	40-41	0.3190540584781729
2228	42-43	0.550501604715123
2228	44-45	0.4764491687011798
2228	46-47	0.3988109247567948
2228	48-49	0.4899616629711332
2228	50-51	0.4348303817691246
2228	52-53	0.4503157879937518
2228	54-55	0.47412032351331135
2228	56-57	0.5339387636374511
2228	58-59	0.4617595858559369
2228	60-61	0.4279064268525232
2228	62-63	0.45842388547719537
2228	64-65	0.2948537880792159
2228	66-67	0.6472504537471693
2228	68-69	0.5954575338733648
2228	70-71	0.6897983291281804
2228	72-73	0.7183117226305882
2228	74-75	0.6157880548742725
2228	76-77	0.7176004553922297
2228	78-79	0.610551206065459
2228	80-81	0.5352019733392979
2228	82-83	0.4883105694086822
2228	84-85	0.5079499235292033
2228	86-87	0.6025700665689158
2228	88-89	0.6791885414804568
2228	90-91	0.6447903400332322
2228	92-93	0.5941989566732246
2228	94-95	0.7542401174350388
2228	96-97	0.573271176892959
2228	98-99	0.8095950834912387
2228	100	0.6991413593350018
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	15.0
13	21.0
14	118.0
15	442.0
16	1641.0
17	4009.0
18	6757.0
19	8497.0
20	9960.0
21	11398.0
22	13397.0
23	15215.0
24	17320.0
25	19459.0
26	21897.0
27	24691.0
28	28125.0
29	32039.0
30	36477.0
31	42074.0
32	49775.0
33	59190.0
34	72933.0
35	93576.0
36	131717.0
37	216590.0
38	530227.0
39	1008334.0
40	1132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.702336751741996	19.65140403900815	19.477491714142865	34.16876749510699
2	19.4363492500232	52.47843195993611	11.461424996621727	16.623793793418958
3	39.99571026110428	16.129988042454578	23.944272465981232	19.930029230459915
4	17.46876101433196	16.365923681719284	22.805945073434305	43.35937023051445
5	17.018338430281162	35.57569191372008	26.4880387916123	20.91793086438646
6	17.292043307641023	27.74472065008673	31.248305878733067	23.714930163539172
7	20.266777803735085	26.784535450581316	36.62366617203074	16.325020573652864
8	20.30271555937951	27.706137419791244	34.84200004395558	17.149146976873666
9	20.594328265146565	28.12261652908842	27.92371753493858	23.359337670826438
10-11	19.26798902412917	30.443104794169862	28.85223843785129	21.43666774384968
12-13	24.08157260037134	26.54660553042581	27.46472768297934	21.907094186223507
14-15	21.809455821794316	27.456669160196107	28.53722345632484	22.196651561684735
16-17	20.81282412151927	29.841381409883333	28.212298119759417	21.133496348837983
18-19	21.322708835803937	29.635787329885805	29.94412757536957	19.097376258940688
20-21	22.68071237341404	29.110477463404944	29.932060140999727	18.276750022181286
22-23	22.042949484458042	30.37379335831204	28.704946549202166	18.878310608027753
24-25	22.217408362793066	28.430855025547146	26.495405421025254	22.85633119063453
26-27	20.049462236052854	26.48171000225476	30.074203528981787	23.394624232710605
28-29	19.372751209750202	28.296016040598847	30.751556105364415	21.579676644286533
30-31	20.035261803525447	30.203914414315403	28.297098706185288	21.463725075973862
32-33	20.348238073182785	30.320415819775615	28.85889282408896	20.472453282952642
34-35	21.28632338444933	29.405122290118218	29.940240762613012	19.36831356281944
36-37	21.89628843976417	28.167976244451626	28.886385410654995	21.04934990512921
38-39	21.50461770701334	29.320155253774967	29.023636253746677	20.15159078546501
40-41	20.804986574060415	29.9772995292018	29.837555797268823	19.380158099468968
42-43	20.32897921856294	29.99235457359094	29.552778167250672	20.125888040595445
44-45	20.47479351052858	30.09314919744439	28.94432130551325	20.487735986513776
46-47	19.744461934352135	28.23100380651391	29.03756554051785	22.9869687186161
48-49	20.69178769206913	28.032147000509354	30.59838003309286	20.677685274328656
50-51	21.82152325616416	30.689072887303592	28.251878490500303	19.237525366031942
52-53	20.41692460411943	30.445064715336766	28.29943211025193	20.838578570291872
54-55	22.462173746143492	28.33884442519441	28.233086823408822	20.96589500525328
56-57	21.66752722249822	28.740604185496625	29.502343649352365	20.08952494265279
58-59	21.160825005378072	28.13799043486773	29.04523095791142	21.65595360184277
60-61	19.5180712594062	30.44507519872626	29.752794336886552	20.284059204980988
62-63	20.377420161706723	31.440208260446855	27.29846546112182	20.8839061167246
64-65	19.74897650447432	30.28441759350042	28.973419437603503	20.993186464421754
66-67	20.102147881219	30.359100799096144	29.524290748246052	20.014460571438804
68-69	20.86823664055651	29.921478242395484	29.44531315500935	19.76497196203866
70-71	19.54431902633509	30.92789819887946	29.398101607390398	20.129681167395052
72-73	19.914787226508796	30.847475769487176	28.741738996656935	20.495998007347094
74-75	19.978868762479518	29.186850281600602	30.591943267999604	20.242337687920276
76-77	19.424784271717108	30.571064368061222	28.78254035569831	21.221611004523353
78-79	19.51709098723416	29.577403739317372	28.507003995887715	22.398501277560758
80-81	20.471652268477857	29.664773046395187	28.550232761448612	21.31334192367834
82-83	20.06220121398797	28.58042609235718	30.138793810077715	21.218578883577138
84-85	20.4838084741472	29.09672099521942	28.3774367874007	22.042033743232672
86-87	21.451848698385774	28.919067197498116	29.135446674149968	20.493637429966146
88-89	20.86982392534715	29.445598052279465	29.129280683232494	20.555297339140896
90-91	20.53181366416147	30.42538011400775	28.62259088833411	20.420215333496675
92-93	20.16661606348488	29.95251169503294	29.768691906394153	20.112180335088027
94-95	20.370297261811636	30.325279423172567	28.8046809435472	20.499742371468596
96-97	20.13507386572222	30.06016216352615	29.20530755474301	20.59945641600862
98-99	21.055475196436667	30.32702950640327	28.708100768978433	19.909394528181632
100	21.297291929348734	32.014842333780756	27.03174488182054	19.656120855049966
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	14.0
2	18.5
3	17.5
4	29.0
5	29.0
6	33.0
7	42.5
8	48.0
9	57.5
10	61.0
11	77.5
12	106.5
13	116.5
14	128.5
15	158.0
16	205.0
17	312.5
18	477.5
19	715.5
20	1087.5
21	1713.5
22	2655.0
23	4141.5
24	6468.0
25	9698.0
26	13970.0
27	18814.0
28	24299.5
29	30614.5
30	38042.0
31	46464.5
32	55458.5
33	65317.5
34	76483.0
35	88788.0
36	100510.0
37	109203.5
38	114918.0
39	118482.0
40	120191.5
41	120502.0
42	118714.5
43	115751.0
44	114086.0
45	111165.5
46	102524.0
47	91987.5
48	85295.5
49	81786.0
50	78965.5
51	73821.0
52	64772.5
53	53780.5
54	43661.5
55	35186.5
56	26916.0
57	19505.0
58	13781.5
59	9977.5
60	8030.0
61	6647.5
62	5362.5
63	4311.0
64	3455.0
65	2906.0
66	2522.0
67	1994.0
68	1501.0
69	1588.0
70	1898.0
71	1730.5
72	1095.5
73	539.5
74	281.5
75	162.5
76	115.0
77	76.0
78	58.5
79	57.5
80	43.5
81	32.5
82	25.5
83	20.0
84	19.5
85	18.5
86	13.5
87	11.5
88	11.5
89	10.0
90	10.0
91	14.0
92	12.5
93	9.0
94	12.0
95	13.5
96	12.5
97	15.0
98	32.5
99	84.5
100	93.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.351813126926617
2	0.006104941502450523
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	4.2734590517153666E-4
30-31	0.010581898604247575
32-33	0.0
34-35	0.0
36-37	0.0
38-39	6.715435652695576E-4
40-41	0.0011599388854655994
42-43	6.104941502450523E-5
44-45	0.0
46-47	0.0021774291358740198
48-49	2.034980500816841E-5
50-51	0.0
52-53	0.0014244863505717888
54-55	0.018925318657596623
56-57	0.01141624060958248
58-59	0.011823236709745848
60-61	1.2209883004901046E-4
62-63	0.011986035149811194
64-65	2.0349805008168412E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.6279844006534728E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2457026.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.2717067942734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.63150502816612	65.20901245626742
2	8.325070419159715	12.532845192653378
3	2.326694257151873	5.254027437727666
4	0.9595124410517615	2.8889655651322292
5	0.5102331856723777	1.9203061374319639
6	0.3158340942118632	1.4264022801090175
7	0.19836654540729431	1.0451971910583546
8	0.14115253749235898	0.8499833932314023
9	0.10515717504925064	0.7123824042859148
>10	0.4574003028798002	5.784546170174812
>50	0.019968944784794704	1.0355822480710641
>100	0.00871991393294789	1.1501111680042562
>500	3.8515503987211877E-4	0.19063835585246122
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	2.0349805008168413E-5	0.0	0.0	0.0	0.0
12-13	4.0699610016336826E-5	0.0	0.0	0.0	0.0
14-15	4.0699610016336826E-5	0.0	0.0	0.0	0.0
16-17	4.0699610016336826E-5	0.0	0.0	0.0	0.0
18-19	4.0699610016336826E-5	0.0	0.0	0.0	0.0
20-21	4.0699610016336826E-5	0.0	0.0	0.0	0.0
22-23	4.0699610016336826E-5	0.0	0.0	0.0	0.0
24-25	4.0699610016336826E-5	0.0	0.0	0.0	0.0
26-27	4.0699610016336826E-5	0.0	0.0	0.0	0.0
28-29	4.0699610016336826E-5	0.0	0.0	0.0	0.0
30-31	4.0699610016336826E-5	0.0	0.0	0.0	0.0
32-33	4.0699610016336826E-5	0.0	0.0	0.0	0.0
34-35	4.0699610016336826E-5	0.0	0.0	0.0	0.0
36-37	4.0699610016336826E-5	0.0	0.0	0.0	0.0
38-39	4.0699610016336826E-5	0.0	0.0	0.0	0.0
40-41	4.0699610016336826E-5	0.0	0.0	0.0	0.0
42-43	4.0699610016336826E-5	0.0	0.0	0.0	0.0
44-45	4.0699610016336826E-5	0.0	0.0	0.0	0.0
46-47	4.0699610016336826E-5	0.0	0.0	0.0	0.0
48-49	8.139922003267365E-5	0.0	0.0	0.0	0.0
50-51	8.139922003267365E-5	0.0	0.0	0.0	0.0
52-53	8.139922003267365E-5	0.0	0.0	4.0699610016336826E-5	0.0
54-55	8.139922003267365E-5	0.0	0.0	4.0699610016336826E-5	0.0
56-57	1.2209883004901046E-4	0.0	0.0	4.0699610016336826E-5	0.0
58-59	1.2209883004901046E-4	0.0	0.0	4.0699610016336826E-5	0.0
60-61	2.44197660098021E-4	0.0	0.0	4.0699610016336826E-5	0.0
62-63	3.866462951551998E-4	0.0	0.0	4.0699610016336826E-5	0.0
64-65	4.0699610016336823E-4	0.0	0.0	4.0699610016336826E-5	0.0
66-67	5.290949302123786E-4	0.0	0.0	4.0699610016336826E-5	0.0
68-69	6.104941502450523E-4	0.0	0.0	4.0699610016336826E-5	0.0
70-71	6.104941502450523E-4	0.0	0.0	4.0699610016336826E-5	0.0
72-73	6.104941502450523E-4	0.0	0.0	4.0699610016336826E-5	0.0
74-75	7.122431752858944E-4	0.0	0.0	4.0699610016336826E-5	0.0
76-77	7.529427853022312E-4	0.0	0.0	4.0699610016336826E-5	0.0
78-79	9.157412253675785E-4	0.0	0.0	4.0699610016336826E-5	0.0
80-81	0.001098889470441094	0.0	0.0	4.0699610016336826E-5	0.0
82-83	0.00128203771551461	0.0	0.0	4.0699610016336826E-5	0.0
84-85	0.001383786740555452	0.0	0.0	4.0699610016336826E-5	0.0
86-87	0.0014651859605881257	0.0	0.0	4.0699610016336826E-5	0.0
88	0.0014651859605881257	0.0	0.0	4.0699610016336826E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	9915	0.0	79.57957	8
CACCGGC	9520	0.0	78.838455	9
CTTCACC	10970	0.0	73.72338	6
GTGTGGC	4310	0.0	71.11521	7
CGGGCCC	615	0.0	70.97975	7
CCTTCAC	12110	0.0	70.310425	5
TTCACCG	12375	0.0	65.54282	7
GCTGCTA	65	1.0913936E-11	64.997986	2
CGCTGCA	75	1.2732926E-11	63.52949	1
CGTAGTG	1805	0.0	62.931015	4
TGTGTGG	4815	0.0	61.219524	6
ACCTTCA	4730	0.0	61.128845	4
CGGGGCA	1010	0.0	60.41553	94
TGTGGCA	5010	0.0	60.054688	8
GCTGCCT	115	0.0	59.84662	1
GGGCCCG	730	0.0	59.798004	8
GCAGCCC	95	0.0	59.290375	4
ACGGGCC	730	0.0	59.155014	6
GCCTTCA	3300	0.0	58.743893	4
CAGCGTA	1135	0.0	58.30533	1
>>END_MODULE