FastQCFastQC Report
Tue 7 Mar 2017
1NF_21A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_21A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3046937
Sequences flagged as poor quality0
Sequence length95
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA133390.4377839121714693No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC94950.3116244280731764No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG82690.271387298129236No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG78760.25848909905258954No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA70620.23177374523989172No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA65130.2137556503465612No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT62380.20473019297740652No Hit
CAGCAGGCGCGCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACGAAA61240.20098873064982964No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA60880.19980721623059486No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG59350.194785779948847No Hit
CAGCAGAAGAGCGGCCGGGAGAGTTGAGGGTGGACGTCTCGAGCTCGAGG58410.19170071452084503No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA57520.1887797483177368No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA56920.18681055761901214No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG52590.1725995647432159No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG49390.16209721435001775No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG45020.14775494209430654No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT39660.1301635051856996No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT39410.129343009061231No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC35630.1169371076592657No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA35280.11578841308500963No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA34480.1131628254867101No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA34210.11227668967228399No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC34040.11171875230764534No Hit
CTGCGGACGAGCCTAAGAGGTAACCCTAGCCTTAGCTGTATCCCAAAGCG31970.1049250443970453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTA500.088.9998863
GCTGCCC3100.088.9998863
GCAGCTT450.088.9998863
TGCAGCG450.088.9998862
GCAGCCA1100.088.999883
CAGCAGC9050.088.0164641
AGCAGCA4000.087.887392
AGCTGCC3350.087.671532
AGCAGCC1800.086.5276642
CAGCTGC8350.085.802281
CTGCAGC2600.085.576811
AGCTGCG1100.084.954432
GCAGCAC1100.084.954433
GCAGCAA2100.084.761793
GCTGCAA1000.084.549893
AGCTGCA2300.083.195552
GCTGCAT700.082.642753
CAGCCCT150550.082.142381
CTGCTGC3650.081.684831
GCAGCCG600.081.583233
Produced by FastQC (version 0.11.5)