FastQCFastQC Report
Tue 7 Mar 2017
1NF_21A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_21A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3046937
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG98250.077.966618
CTTCACC113450.070.582266
CACCGGC104100.070.564249
CCTTCAC124550.068.248925
TTCACCG122650.064.024977
GTGTGGC45200.062.721957
GCTGCCT1300.061.0821231
AGGCGCG11150.061.0399485
CGGGGCA14250.060.2777894
CAGCGTA11550.060.0420571
CGGGCCC8150.059.8958557
CGTAGTG20200.059.252934
GGGCCCG8350.059.023358
GCGTAGT13450.058.977383
CGAGCGA10000.058.2026447
GCGCGCA12100.056.635477
ACCTTCA50250.056.231734
GCAGCCA851.8189894E-1255.2234462
CAGCAGC1250.055.0553551
TGTGTGG49350.053.4527666
Produced by FastQC (version 0.11.5)