##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1NF_21A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3046937
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.752863613524008	12.0	12.0	12.0	2.0	27.0
2	14.70876293142917	12.0	12.0	12.0	12.0	22.0
3	13.67423711090843	12.0	12.0	12.0	12.0	22.0
4	13.692939499569567	12.0	12.0	12.0	12.0	22.0
5	22.457905759127936	22.0	22.0	22.0	22.0	27.0
6	26.889083692902084	27.0	22.0	32.0	22.0	32.0
7	35.00466763835288	37.0	37.0	37.0	27.0	41.0
8	37.64979518775741	41.0	37.0	41.0	32.0	41.0
9	38.58616702609867	41.0	41.0	41.0	32.0	41.0
10-11	38.92842057449826	41.0	41.0	41.0	37.0	41.0
12-13	39.10858117512768	41.0	41.0	41.0	37.0	41.0
14-15	39.06200653311834	41.0	41.0	41.0	37.0	41.0
16-17	38.977079933060644	41.0	41.0	41.0	37.0	41.0
18-19	38.912593040158036	41.0	41.0	41.0	37.0	41.0
20-21	38.90991477670855	41.0	41.0	41.0	37.0	41.0
22-23	38.909366357098946	41.0	41.0	41.0	37.0	41.0
24-25	38.93199760940249	41.0	41.0	41.0	37.0	41.0
26-27	38.89917776442375	41.0	41.0	41.0	37.0	41.0
28-29	38.84566533538435	41.0	41.0	41.0	37.0	41.0
30-31	38.7334619980656	41.0	41.0	41.0	32.0	41.0
32-33	38.76711399021378	41.0	41.0	41.0	32.0	41.0
34-35	38.74318093875915	41.0	41.0	41.0	32.0	41.0
36-37	38.67136898465574	41.0	41.0	41.0	32.0	41.0
38-39	38.57616238865457	41.0	41.0	41.0	32.0	41.0
40-41	38.62269124698017	41.0	41.0	41.0	32.0	41.0
42-43	38.36561093977329	41.0	41.0	41.0	32.0	41.0
44-45	38.463275578064135	41.0	41.0	41.0	32.0	41.0
46-47	38.477422408143	41.0	41.0	41.0	32.0	41.0
48-49	38.43331270059079	41.0	41.0	41.0	32.0	41.0
50-51	38.33694378977971	41.0	41.0	41.0	32.0	41.0
52-53	38.29227204238224	41.0	41.0	41.0	32.0	41.0
54-55	38.17707668389599	41.0	41.0	41.0	32.0	41.0
56-57	37.96991700189403	41.0	37.0	41.0	29.5	41.0
58-59	37.95343782953175	41.0	37.0	41.0	29.5	41.0
60-61	37.970463616412154	41.0	37.0	41.0	32.0	41.0
62-63	37.949847994887975	41.0	37.0	41.0	32.0	41.0
64-65	37.92763552380637	41.0	37.0	41.0	32.0	41.0
66-67	37.797978428828685	41.0	37.0	41.0	27.0	41.0
68-69	37.81598159069256	41.0	37.0	41.0	27.0	41.0
70-71	37.78398125724293	41.0	37.0	41.0	27.0	41.0
72-73	37.63940179924954	41.0	37.0	41.0	27.0	41.0
74-75	37.556512983366574	41.0	37.0	41.0	27.0	41.0
76-77	36.49936542829734	39.0	34.5	41.0	24.5	41.0
78-79	37.02517364159482	41.0	37.0	41.0	27.0	41.0
80-81	37.68469023153416	41.0	37.0	41.0	27.0	41.0
82-83	37.856517545325026	41.0	37.0	41.0	32.0	41.0
84-85	37.769721855095796	41.0	37.0	41.0	27.0	41.0
86-87	37.639215874827734	41.0	37.0	41.0	27.0	41.0
88-89	37.56814154674022	41.0	37.0	41.0	27.0	41.0
90-91	37.41358469177406	41.0	37.0	41.0	27.0	41.0
92-93	37.29276614514839	41.0	37.0	41.0	27.0	41.0
94-95	37.12807271696133	41.0	37.0	41.0	27.0	41.0
96-97	37.054328330385566	41.0	37.0	41.0	27.0	41.0
98-99	36.941722129469696	41.0	37.0	41.0	27.0	41.0
100	36.792485371374596	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.3827438052452052
1101	2	-0.5628205497252488
1101	3	0.004178101258604627
1101	4	0.017881627239070497
1101	5	-1.1098265102152958
1101	6	-0.8325525039386967
1101	7	-0.370227723500264
1101	8	-0.2515578029225978
1101	9	-0.02543335843811434
1101	10-11	-0.12554867240071843
1101	12-13	-0.13858745845335818
1101	14-15	-0.07354894226661912
1101	16-17	-0.17919180349583996
1101	18-19	-0.09669313348017994
1101	20-21	-0.0942607329869034
1101	22-23	-0.06559254872536968
1101	24-25	0.014968855424065453
1101	26-27	-0.008321910472808725
1101	28-29	-0.028752630906708987
1101	30-31	-0.10935114812215829
1101	32-33	-0.236375504823215
1101	34-35	-0.06897024820073483
1101	36-37	-0.06863324304281804
1101	38-39	-0.14468615139342234
1101	40-41	-0.09574606319971934
1101	42-43	0.012962569780512467
1101	44-45	-0.09425989176254035
1101	46-47	-0.17460079667154105
1101	48-49	-0.07979243528053814
1101	50-51	-0.06412626809616029
1101	52-53	-0.03475904363873639
1101	54-55	-0.09661616334954459
1101	56-57	0.032060676267434474
1101	58-59	-0.20068187618421263
1101	60-61	-0.05115758592057773
1101	62-63	-0.22933565078044182
1101	64-65	-0.14096762714896016
1101	66-67	-0.20027754174025603
1101	68-69	-0.09691386911333666
1101	70-71	-0.27023503335151844
1101	72-73	-0.1039182627790396
1101	74-75	-0.2452474066479553
1101	76-77	-0.6084708238773828
1101	78-79	-0.20945461317852931
1101	80-81	-0.1736112322304919
1101	82-83	-0.15181179307281667
1101	84-85	-0.27161483709418377
1101	86-87	-0.1797040200967004
1101	88-89	-0.4406688202704956
1101	90-91	-0.31489028603854763
1101	92-93	-0.08480486663590625
1101	94-95	-0.11549737773101754
1101	96-97	-0.20637880875314352
1101	98-99	-0.3942073441835561
1101	100	-0.5323212733087317
1102	1	1.5064001871891897
1102	2	0.8414171318186199
1102	3	-0.037650214058501774
1102	4	0.08744373900598568
1102	5	-0.8031914548402739
1102	6	-0.36743047993180156
1102	7	-0.21069155841416176
1102	8	-0.4915091977984716
1102	9	-0.47150101323396854
1102	10-11	-0.3667810292104079
1102	12-13	-0.34166458558380697
1102	14-15	-0.3374936252694667
1102	16-17	-0.37251391813651225
1102	18-19	-0.3213708033079783
1102	20-21	-0.21741572779204432
1102	22-23	-0.22617335094479785
1102	24-25	-0.25780543608509987
1102	26-27	-0.2554619728111618
1102	28-29	-0.45470753231484906
1102	30-31	-0.33303129379936536
1102	32-33	-0.3604277877995372
1102	34-35	-0.31132419795424937
1102	36-37	-0.4272609288973115
1102	38-39	-0.21189820955377314
1102	40-41	-0.2651468717192955
1102	42-43	-0.2803713576461533
1102	44-45	-0.14407143983159898
1102	46-47	-0.5500751732880218
1102	48-49	-0.41955662167662666
1102	50-51	-0.371224854430352
1102	52-53	-0.4176776169305114
1102	54-55	-0.3372051723466072
1102	56-57	-0.30032176233370933
1102	58-59	-0.23415751559442555
1102	60-61	-0.25640874923820434
1102	62-63	-0.479796211272955
1102	64-65	-0.43222153711204214
1102	66-67	-0.35787295589238965
1102	68-69	-0.465436317551557
1102	70-71	-0.4589116144687466
1102	72-73	-0.39460868212604083
1102	74-75	-0.46024398024921
1102	76-77	-0.3278906295674133
1102	78-79	-0.42944702812646796
1102	80-81	-0.4399668288659839
1102	82-83	-0.4274092713538735
1102	84-85	-0.4708459697952918
1102	86-87	-0.4088583904130374
1102	88-89	-0.43512814007507217
1102	90-91	-0.5469304992489796
1102	92-93	-0.3551497309063052
1102	94-95	-0.3646282053721066
1102	96-97	-0.175054132603087
1102	98-99	-0.504225899238044
1102	100	-0.7814735159419754
1103	1	1.4560066027306124
1103	2	0.797754214531599
1103	3	0.3771855103291042
1103	4	0.9433278553486346
1103	5	2.8591901040640018
1103	6	0.09895849740840745
1103	7	-0.18182965434893816
1103	8	0.09367401117708596
1103	9	-0.11209141860874894
1103	10-11	-0.1517708883863449
1103	12-13	-0.27860766500926104
1103	14-15	-0.08116428802323838
1103	16-17	-0.2540851577841181
1103	18-19	-0.08826531578821317
1103	20-21	-0.1906965210425824
1103	22-23	-0.005009365070229421
1103	24-25	-0.1452733426001842
1103	26-27	-0.16864970571304383
1103	28-29	-0.20861483342130072
1103	30-31	-0.18160361781231416
1103	32-33	-0.40448142085374883
1103	34-35	-0.3271917906344939
1103	36-37	-0.19865541412499255
1103	38-39	-0.24513658920212578
1103	40-41	-0.409134309719704
1103	42-43	-0.23097686668109674
1103	44-45	-0.4567767305591275
1103	46-47	-0.4386176915974431
1103	48-49	-0.3298878551278648
1103	50-51	-0.2177550287607275
1103	52-53	-0.20650915589737195
1103	54-55	-0.2229632670765298
1103	56-57	-0.3021964521564513
1103	58-59	-0.5619470311011341
1103	60-61	-0.33923712116978777
1103	62-63	-0.4623803858500395
1103	64-65	-0.3769338934264681
1103	66-67	-0.4045124990819744
1103	68-69	-0.5483844573486252
1103	70-71	-0.5330493575544821
1103	72-73	-0.45880712672157387
1103	74-75	-0.46712661634710173
1103	76-77	-0.6428809047036097
1103	78-79	-0.4978035691731364
1103	80-81	-0.21964709886722034
1103	82-83	-0.305088342242108
1103	84-85	-0.3790828433806652
1103	86-87	-0.3246158970612285
1103	88-89	-0.4182928660720151
1103	90-91	-0.6138448774170939
1103	92-93	-0.2326200327786978
1103	94-95	-0.3321762057507698
1103	96-97	-0.16660978091299228
1103	98-99	-0.341845702962182
1103	100	-0.24956420101416654
1104	1	1.4516643082290077
1104	2	-0.6214364152464853
1104	3	-0.2342050167600629
1104	4	0.10677201895874511
1104	5	-1.100235717376421
1104	6	-0.4805190372632886
1104	7	-0.2645254370115282
1104	8	-0.2273894148315776
1104	9	0.002893362439756686
1104	10-11	-0.09335195731557633
1104	12-13	-0.017510666532579933
1104	14-15	-0.06775969652372993
1104	16-17	-0.016748091339849225
1104	18-19	0.09455861526837595
1104	20-21	0.03960514892884959
1104	22-23	0.1278025099670046
1104	24-25	-0.007105474586644789
1104	26-27	-0.027189267895160185
1104	28-29	0.004081147811021424
1104	30-31	0.011543528031474182
1104	32-33	0.046856797294957175
1104	34-35	-0.007205747249848571
1104	36-37	-0.05161757808117784
1104	38-39	-0.04180515646478966
1104	40-41	-0.0214808791439296
1104	42-43	0.1936667006524857
1104	44-45	0.04018444338511529
1104	46-47	-0.07917903404229065
1104	48-49	-0.14723483587511055
1104	50-51	-0.017205841620103968
1104	52-53	-0.07715991145520462
1104	54-55	-0.07446771645685857
1104	56-57	-0.07291649436670156
1104	58-59	-0.022745891310883337
1104	60-61	0.024448942922845163
1104	62-63	-0.05313809957489468
1104	64-65	-0.0603868650224797
1104	66-67	-0.5224561818696571
1104	68-69	-0.30145952238022033
1104	70-71	-0.20661419482658516
1104	72-73	-0.07485269563802888
1104	74-75	-0.22665165173506807
1104	76-77	-0.22566880953764468
1104	78-79	-0.06837603152290228
1104	80-81	-0.11002448064022019
1104	82-83	-0.050098497665473474
1104	84-85	0.10052035756338995
1104	86-87	-0.06752603574800986
1104	88-89	-0.2042116482154981
1104	90-91	-0.07707189084324284
1104	92-93	-0.06847449251301185
1104	94-95	-0.004377932740084134
1104	96-97	-0.017602700483749345
1104	98-99	-0.11026666228091386
1104	100	-0.1594640367686324
1105	1	1.3440291669260027
1105	2	0.4505504155560107
1105	3	-0.0513503624425482
1105	4	-0.13749795234378226
1105	5	-1.1538787735808143
1105	6	0.5116332511339863
1105	7	0.08192367244159016
1105	8	-0.1764497836126111
1105	9	-0.2022578359134357
1105	10-11	-0.22178522787877597
1105	12-13	-0.14506916191651698
1105	14-15	-0.18629624246495524
1105	16-17	-0.14389959287316856
1105	18-19	0.021547691008194647
1105	20-21	-0.0764074430974091
1105	22-23	-0.24836844066792452
1105	24-25	-0.0976711065444178
1105	26-27	-0.20117383820203116
1105	28-29	-0.2683692236534725
1105	30-31	-0.12981365602267658
1105	32-33	-0.16529246480839532
1105	34-35	-0.12815086792917185
1105	36-37	-0.14351957076767974
1105	38-39	-0.17773277269106558
1105	40-41	-0.038830988444367165
1105	42-43	-0.038106521713068275
1105	44-45	-0.17083890795590406
1105	46-47	-0.29432739748229153
1105	48-49	-0.2449971407269942
1105	50-51	-0.12748919663177816
1105	52-53	-0.12743852187686144
1105	54-55	-0.1931764362174917
1105	56-57	-0.1673217883708844
1105	58-59	-0.10039772955037307
1105	60-61	-0.14409621515143556
1105	62-63	-0.2783116467920337
1105	64-65	-0.19893674152683616
1105	66-67	-0.12499598321095107
1105	68-69	-0.3378342535694543
1105	70-71	-0.3975008507566571
1105	72-73	-0.3466451441948166
1105	74-75	-0.27746413179670526
1105	76-77	-0.3941127010432197
1105	78-79	-0.17754634922455637
1105	80-81	-0.28195466825254556
1105	82-83	-0.21572537490050792
1105	84-85	-0.2088874925439157
1105	86-87	-0.24563788993956592
1105	88-89	-0.23568317743981027
1105	90-91	-0.2574059645033486
1105	92-93	-0.21015977749613057
1105	94-95	-0.06404190947479549
1105	96-97	-0.12964634520454865
1105	98-99	-0.3914250637093062
1105	100	-0.5841727215575361
1106	1	1.1953116869030378
1106	2	-0.5976194027967576
1106	3	-0.025336843567183465
1106	4	-0.24971058320493
1106	5	-1.1755359549976419
1106	6	-0.7407699859093313
1106	7	-0.4017519653979065
1106	8	-0.08871405020732936
1106	9	-0.2696302912732875
1106	10-11	-0.20971568300111443
1106	12-13	-0.10638005555127705
1106	14-15	-0.14543751859817888
1106	16-17	-0.1906122136096684
1106	18-19	-0.11745025833898382
1106	20-21	-0.14030187470208233
1106	22-23	0.01439539549219404
1106	24-25	-0.13960283846767396
1106	26-27	-0.2190482985169524
1106	28-29	-0.1975593799726525
1106	30-31	-0.05355948507583719
1106	32-33	-0.16400198192808801
1106	34-35	-0.15440040069008631
1106	36-37	-0.5299105586219284
1106	38-39	-0.3399157000075448
1106	40-41	-0.24554268975285964
1106	42-43	0.03333385529619193
1106	44-45	-0.19748562814276482
1106	46-47	-0.3909475144576149
1106	48-49	-0.31286112065719607
1106	50-51	-0.429739592451007
1106	52-53	-0.3639459842938706
1106	54-55	-0.44629250360500095
1106	56-57	-0.2396492355407105
1106	58-59	-0.266325068468511
1106	60-61	-0.2821591390529079
1106	62-63	-0.31399195237050037
1106	64-65	-0.6023959115350976
1106	66-67	-0.5161015707610233
1106	68-69	-0.589263334748928
1106	70-71	-0.685747892852774
1106	72-73	-0.5381860097596629
1106	74-75	-0.5330091583205601
1106	76-77	-0.4570301035715332
1106	78-79	-0.4802141817770149
1106	80-81	-0.5212676858754648
1106	82-83	-0.5090607395494899
1106	84-85	-0.5918984195079489
1106	86-87	-0.4547262852804792
1106	88-89	-0.6827016858539494
1106	90-91	-0.5834542902960891
1106	92-93	-0.6279485476681401
1106	94-95	-0.4937410348689042
1106	96-97	-0.668216555299324
1106	98-99	-0.6743541314804489
1106	100	-0.8445595216361284
1107	1	1.4956767134592628
1107	2	-0.539890655691666
1107	3	-0.19437758088638546
1107	4	-0.24217923708409472
1107	5	-1.224669551606354
1107	6	-0.3444814688304092
1107	7	-0.44664825974785316
1107	8	-0.4921296399688089
1107	9	-0.36573682280995
1107	10-11	-0.3069414311777834
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2221	7	0.35588728486632704
2221	8	0.17326655242128908
2221	9	0.2880931572009615
2221	10-11	0.17986507132434326
2221	12-13	0.2762621575418365
2221	14-15	0.16161498380709105
2221	16-17	0.10105930132245788
2221	18-19	0.15751992482848465
2221	20-21	0.14224385790853233
2221	22-23	0.19588442171269804
2221	24-25	0.21685200748138556
2221	26-27	0.17424663116704409
2221	28-29	0.19221840247382005
2221	30-31	0.17277734331818806
2221	32-33	0.29612130407540604
2221	34-35	0.31215626422043385
2221	36-37	0.3319884854078694
2221	38-39	0.2853830070139267
2221	40-41	0.3305329248725357
2221	42-43	0.47519695751810076
2221	44-45	0.3039219849268662
2221	46-47	0.4037505301830606
2221	48-49	0.34490668944594205
2221	50-51	0.35353661215441434
2221	52-53	0.2693572089892342
2221	54-55	0.2646261672807313
2221	56-57	0.47229255402947956
2221	58-59	0.5323827610492415
2221	60-61	0.5115444724739007
2221	62-63	0.42983903386366507
2221	64-65	0.3851705770386218
2221	66-67	0.3096462472501784
2221	68-69	0.47530263236690473
2221	70-71	0.48449369621471305
2221	72-73	0.381339921457446
2221	74-75	0.4222001412990153
2221	76-77	0.7309033448273041
2221	78-79	0.4625236283368963
2221	80-81	0.3941914933455237
2221	82-83	0.334982008145694
2221	84-85	0.33585582622584553
2221	86-87	0.525612247548203
2221	88-89	0.3965348631722563
2221	90-91	0.3704661787574324
2221	92-93	0.07916692831840066
2221	94-95	0.3267359105514771
2221	96-97	0.4836420272617943
2221	98-99	0.5457703181067473
2221	100	0.6644908432227794
2222	1	1.3770855815398164
2222	2	1.711864515836913
2222	3	0.6042770883178576
2222	4	0.7564330629538158
2222	5	3.187472672135499
2222	6	0.11784612687444351
2222	7	0.36805880782149814
2222	8	0.2582136147652392
2222	9	0.1716659719908833
2222	10-11	0.12353650207403177
2222	12-13	0.18939288362874862
2222	14-15	0.12831350327708435
2222	16-17	0.18609324704389962
2222	18-19	0.24875305940777537
2222	20-21	0.1462770162244169
2222	22-23	0.2822046538842429
2222	24-25	0.17823600513327875
2222	26-27	0.27077599282568343
2222	28-29	0.2247042037607585
2222	30-31	0.29204553514071563
2222	32-33	0.18049675894913264
2222	34-35	0.3687271223381501
2222	36-37	0.2723438450744453
2222	38-39	0.29190371758956246
2222	40-41	0.32285525501819023
2222	42-43	0.4986741633380376
2222	44-45	0.3602044514355143
2222	46-47	0.33949701273679267
2222	48-49	0.41268749905000135
2222	50-51	0.4530267943327573
2222	52-53	0.501775765469155
2222	54-55	0.3525323869863044
2222	56-57	0.46133220930604324
2222	58-59	0.6318000969041933
2222	60-61	0.3579417793819175
2222	62-63	0.42250171267643566
2222	64-65	0.44602932276198004
2222	66-67	0.5530296722434827
2222	68-69	0.5792058381639578
2222	70-71	0.5281927087778016
2222	72-73	0.5931338543100395
2222	74-75	0.5850893103080708
2222	76-77	0.7419295506102443
2222	78-79	0.7235550896993388
2222	80-81	0.5445277439911607
2222	82-83	0.48518301077721304
2222	84-85	0.45957775577659987
2222	86-87	0.4651174008102217
2222	88-89	0.37572802394694804
2222	90-91	0.4532052324497613
2222	92-93	0.6141491298660213
2222	94-95	0.6568928780794465
2222	96-97	0.6478346465122229
2222	98-99	0.6196527889795291
2222	100	0.7544160702970757
2223	1	1.4096532671362993
2223	2	-1.1985902254032403
2223	3	-0.0935978080854678
2223	4	-0.07647888644928891
2223	5	-0.9779234212302512
2223	6	0.1480792170672558
2223	7	0.26541074528856257
2223	8	0.29622372957386744
2223	9	0.08944336063989766
2223	10-11	0.08830878336382142
2223	12-13	0.14022236117732945
2223	14-15	0.05787042172806878
2223	16-17	0.16267176276096507
2223	18-19	0.1579641755468586
2223	20-21	0.20989879316879012
2223	22-23	0.2794914772375279
2223	24-25	0.2889153176579029
2223	26-27	0.25004855351759403
2223	28-29	0.2181927923827871
2223	30-31	0.07111803700273356
2223	32-33	0.14688878869463196
2223	34-35	0.0810044476135019
2223	36-37	0.12056963494013218
2223	38-39	0.22584437964638227
2223	40-41	0.2523020334970809
2223	42-43	0.36871331417877684
2223	44-45	0.3221008040088478
2223	46-47	0.2897576551942791
2223	48-49	0.24124725881996767
2223	50-51	0.3068103348771558
2223	52-53	0.33518493905266666
2223	54-55	0.45497438547012337
2223	56-57	0.47563167258293504
2223	58-59	0.43860575073836827
2223	60-61	0.4516755456933268
2223	62-63	0.3711887751224765
2223	64-65	0.33919268624806875
2223	66-67	0.4533258278668768
2223	68-69	0.5305849527023483
2223	70-71	0.43638154263065587
2223	72-73	0.5564642186913247
2223	74-75	0.6853404002155017
2223	76-77	0.4709283556779056
2223	78-79	0.6016536566042632
2223	80-81	0.3622225741616987
2223	82-83	0.27223481482744205
2223	84-85	0.3473317054704026
2223	86-87	0.3791320944656533
2223	88-89	0.4814315074789661
2223	90-91	0.4390564975182798
2223	92-93	0.5039503013089188
2223	94-95	0.6223145733422086
2223	96-97	0.7253725890018785
2223	98-99	0.7070786172148402
2223	100	0.6721601113791564
2224	1	1.2188874073505147
2224	2	-0.9387022555428892
2224	3	-0.08995815060187518
2224	4	-0.3146731355901533
2224	5	-1.0549558270685502
2224	6	0.674114486289735
2224	7	0.4110512622414504
2224	8	0.23619228336054476
2224	9	0.18325130904823794
2224	10-11	0.2623919782819968
2224	12-13	0.279698095592245
2224	14-15	0.20078243082166836
2224	16-17	0.20893267071628685
2224	18-19	0.15625491867798047
2224	20-21	0.15006318469897906
2224	22-23	0.2690623996126007
2224	24-25	0.2754523241140703
2224	26-27	0.29775404025951957
2224	28-29	0.26029824109225785
2224	30-31	0.25517987605869763
2224	32-33	0.18394558978624076
2224	34-35	0.2325489417084583
2224	36-37	0.1164441556284217
2224	38-39	0.1988773287291039
2224	40-41	0.16490498352259664
2224	42-43	0.38343506433388796
2224	44-45	0.36866478305788064
2224	46-47	0.2364298958584996
2224	48-49	0.16588504913457314
2224	50-51	0.13316214947109017
2224	52-53	0.24163038451977314
2224	54-55	0.17378063123187104
2224	56-57	0.3721331945486739
2224	58-59	0.3917680795956855
2224	60-61	0.3817774293946883
2224	62-63	0.3527675411564033
2224	64-65	0.3980812225889565
2224	66-67	0.31027313342529794
2224	68-69	0.29341055548598405
2224	70-71	0.47301034510789464
2224	72-73	0.4036749230101222
2224	74-75	0.2715540774220173
2224	76-77	0.46131861830212983
2224	78-79	0.3298901607596534
2224	80-81	0.3960524927204361
2224	82-83	0.2075578142310448
2224	84-85	0.22083093934620734
2224	86-87	0.38214117177542306
2224	88-89	0.40076178112298777
2224	90-91	0.32859459553783665
2224	92-93	0.29122898062136926
2224	94-95	0.4502796288912094
2224	96-97	0.3506312243580112
2224	98-99	0.42904050143433636
2224	100	0.7324320647701725
2225	1	1.2991021027278489
2225	2	1.6909171510031253
2225	3	0.8675896316926472
2225	4	0.8863606173144625
2225	5	3.0313009762608267
2225	6	-0.2170256052719175
2225	7	0.0900498886307588
2225	8	0.3934700434802636
2225	9	0.2937188969607831
2225	10-11	0.11937707939891595
2225	12-13	0.18200929581677627
2225	14-15	0.12145827224119898
2225	16-17	0.21562812664855358
2225	18-19	0.09944590685717714
2225	20-21	0.2365241841660577
2225	22-23	0.18553861155915996
2225	24-25	0.19799945452105305
2225	26-27	0.27082896059248895
2225	28-29	0.2854944247222093
2225	30-31	0.26101692488547457
2225	32-33	0.053013977436016546
2225	34-35	0.1578425632743361
2225	36-37	0.2033567631334421
2225	38-39	0.1599429803099568
2225	40-41	0.13787900069259962
2225	42-43	0.32964285793487136
2225	44-45	0.367678388256202
2225	46-47	0.2970806628420348
2225	48-49	0.36491248868760806
2225	50-51	0.0705707225755674
2225	52-53	0.164293752715146
2225	54-55	0.10205594126347961
2225	56-57	0.2635112715308878
2225	58-59	0.3971335389028994
2225	60-61	0.2897847459361387
2225	62-63	0.22518007205467683
2225	64-65	0.2779017716243075
2225	66-67	0.352991881525071
2225	68-69	0.37737586126107203
2225	70-71	0.3217203911064672
2225	72-73	0.431469031168227
2225	74-75	0.30106174699177757
2225	76-77	0.48146214243006824
2225	78-79	0.33969788181657634
2225	80-81	0.26315121079885984
2225	82-83	0.08569925497420172
2225	84-85	0.1691519239911301
2225	86-87	0.21218990800313975
2225	88-89	0.3299638734297261
2225	90-91	0.3272891608669255
2225	92-93	0.30084531758694766
2225	94-95	0.5247065985010408
2225	96-97	0.48851552932242726
2225	98-99	0.4649458962219484
2225	100	0.531362331921919
2226	1	1.4200998502833073
2226	2	1.5968891856973055
2226	3	0.7094771422723909
2226	4	0.8994291698368944
2226	5	3.0635830282725856
2226	6	-0.5720999823186972
2226	7	0.09454344697666528
2226	8	0.323907241173643
2226	9	0.22869049634397243
2226	10-11	0.12123321175032231
2226	12-13	0.08435971168106704
2226	14-15	0.12055826939533887
2226	16-17	0.014098503893492875
2226	18-19	0.13826223513014924
2226	20-21	0.1437615720049621
2226	22-23	0.1714584616351047
2226	24-25	0.1915895528296616
2226	26-27	0.07289223457384963
2226	28-29	0.10933576493197705
2226	30-31	0.2209998023016837
2226	32-33	0.19989178047519118
2226	34-35	0.12406880694097566
2226	36-37	0.08632464076573143
2226	38-39	0.0949755812035491
2226	40-41	0.04187461279993698
2226	42-43	0.23625731890287227
2226	44-45	0.1306609062509878
2226	46-47	0.10470153015438655
2226	48-49	0.186346574908697
2226	50-51	0.09072164268387439
2226	52-53	-0.005517456174054303
2226	54-55	0.12335772140085766
2226	56-57	0.17162073063853
2226	58-59	0.13827158729725397
2226	60-61	0.22120600471218665
2226	62-63	0.07109434108530621
2226	64-65	0.09682162064622446
2226	66-67	0.262636128094492
2226	68-69	0.1691555717634472
2226	70-71	0.34705775712711784
2226	72-73	0.12927914282074937
2226	74-75	0.4184245686923944
2226	76-77	0.4118214742645776
2226	78-79	0.3148076845353174
2226	80-81	0.2794062029095059
2226	82-83	0.07053306736043652
2226	84-85	0.2300585724442996
2226	86-87	0.21662325535496052
2226	88-89	0.24267361058831938
2226	90-91	0.18224693675449544
2226	92-93	0.14491755180745258
2226	94-95	0.3837732147854638
2226	96-97	0.1765780475701746
2226	98-99	0.32394676789795795
2226	100	0.19327167129269185
2227	1	1.475031928380428
2227	2	1.7847522446646291
2227	3	0.6231277822046604
2227	4	-0.08495542113081456
2227	5	3.2801869924090035
2227	6	0.04151864617999479
2227	7	0.2151423464084843
2227	8	0.1874967683690798
2227	9	0.07069089837308695
2227	10-11	0.16977216465309652
2227	12-13	0.12189123135652125
2227	14-15	0.21364990293232466
2227	16-17	0.11335099111182245
2227	18-19	0.0780570951932944
2227	20-21	0.10087042758543419
2227	22-23	0.22448642529296592
2227	24-25	0.27582832740611707
2227	26-27	0.17926928668727982
2227	28-29	0.055338296745361504
2227	30-31	0.17480572732702626
2227	32-33	0.17338824008607645
2227	34-35	0.21607688848067852
2227	36-37	0.17337155637788015
2227	38-39	0.12092687047212536
2227	40-41	0.18898378903364943
2227	42-43	0.31040312828825734
2227	44-45	0.2343477045525475
2227	46-47	0.17036491591856873
2227	48-49	0.1711840549082808
2227	50-51	0.23295392551405314
2227	52-53	0.2572986365233021
2227	54-55	0.21797265996207926
2227	56-57	0.20545267977375659
2227	58-59	0.3504574077411391
2227	60-61	0.32197607100387415
2227	62-63	0.2706107879023989
2227	64-65	0.14983799767409067
2227	66-67	0.27120592298958
2227	68-69	0.3348902557206017
2227	70-71	0.42060577724151216
2227	72-73	0.3606490543366263
2227	74-75	0.3857868508166007
2227	76-77	0.6156260520073644
2227	78-79	0.4090162826770367
2227	80-81	0.23145109490325666
2227	82-83	0.15532220686184672
2227	84-85	0.15851194056985918
2227	86-87	0.20814783760305744
2227	88-89	0.26369761808567205
2227	90-91	0.32133533573551887
2227	92-93	0.1973952852686196
2227	94-95	0.4340554839541966
2227	96-97	0.3124575563186198
2227	98-99	0.5109631945058197
2227	100	0.2608947469016485
2228	1	1.4641334655570155
2228	2	1.5044844619622548
2228	3	-0.15931962557547408
2228	4	-0.05467538955233486
2228	5	3.0535787352222883
2228	6	0.19222017946933434
2228	7	0.2635204804842104
2228	8	0.26993447153002137
2228	9	0.1598127761075716
2228	10-11	0.13407455302871796
2228	12-13	0.23729339334051502
2228	14-15	0.13783786318469282
2228	16-17	0.08462545326468529
2228	18-19	0.014767456041816729
2228	20-21	0.0328545444036763
2228	22-23	0.13077186014426445
2228	24-25	0.25398159641181905
2228	26-27	0.16546537695401042
2228	28-29	0.15829803013423316
2228	30-31	0.2380656722334038
2228	32-33	0.243232816357974
2228	34-35	0.11016547964126744
2228	36-37	0.16683571805602782
2228	38-39	0.19100704358417175
2228	40-41	0.2594590980663938
2228	42-43	0.3799176935179389
2228	44-45	0.3464774932388508
2228	46-47	0.2701810410544425
2228	48-49	0.21173600073842636
2228	50-51	0.18485174545705974
2228	52-53	0.1665284582376998
2228	54-55	0.24769180774264754
2228	56-57	0.34890294589493465
2228	58-59	0.39694604301117664
2228	60-61	0.2798729383015086
2228	62-63	0.3082975090274118
2228	64-65	0.2955556218699371
2228	66-67	0.4687370354723299
2228	68-69	0.45714780659595533
2228	70-71	0.49314152945005674
2228	72-73	0.5085629728796377
2228	74-75	0.3042711539662335
2228	76-77	0.4333376029202398
2228	78-79	0.4634396926480733
2228	80-81	0.32763237449036353
2228	82-83	0.28259376599732633
2228	84-85	0.2747468911552673
2228	86-87	0.26816287638846603
2228	88-89	0.27751610499381485
2228	90-91	0.3833033110320656
2228	92-93	0.4714796463490245
2228	94-95	0.5963457362918447
2228	96-97	0.44005764178405116
2228	98-99	0.5120437950800252
2228	100	0.7310292874770141
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	27.0
13	49.0
14	193.0
15	572.0
16	1922.0
17	4768.0
18	7870.0
19	10071.0
20	11883.0
21	13762.0
22	15834.0
23	18000.0
24	20804.0
25	23535.0
26	27007.0
27	30240.0
28	34204.0
29	38814.0
30	44342.0
31	51231.0
32	60888.0
33	72407.0
34	88895.0
35	115846.0
36	162205.0
37	269725.0
38	663316.0
39	1257021.0
40	1506.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.102793054531347	19.00630517770887	19.080677547466525	37.81022422029325
2	18.16699756690678	46.6794497329768	19.658887748507503	15.494664951608916
3	40.738945373665416	16.874520214891216	23.095062352782485	19.291472058660876
4	17.118601401998138	16.084054248578163	22.339254142766983	44.45809020665672
5	17.883139690777984	36.073144932107226	25.915928028705547	20.12778734840924
6	19.07456570319636	28.44322019129375	29.988936430257667	22.49327767525223
7	20.659206278305064	26.60396325883994	34.94381406638864	17.793016396466353
8	20.860457567714725	27.784722821640223	33.2335391247013	18.121280485943753
9	21.587482773683867	27.978359907014816	27.074140358005433	23.360016961295884
10-11	20.20668625573814	29.400066361726545	28.487198783565265	21.90604859897005
12-13	24.20199367430308	26.713975379208694	26.980029452528886	22.104001493959345
14-15	22.18665827353831	27.380546430726987	28.08376083916405	22.34903445657065
16-17	20.955306919703297	28.987028612669054	28.40628145577017	21.65138301185748
18-19	21.650578925655502	29.08120515783556	29.454760633383625	19.813455283125318
20-21	22.88112619328854	28.57070887911368	29.675851519082936	18.872313408514845
22-23	21.617217553234607	29.35082018433594	28.772780664647808	20.25918159778164
24-25	22.218953000997395	28.25921573042042	26.48351442776795	23.038316840814236
26-27	20.712784675232864	26.656901668790656	29.567398341350675	23.062915314625805
28-29	20.117426566550446	27.690831298632013	30.29299534891812	21.898746785899426
30-31	20.66100552219249	29.464477965060247	28.102918122927207	21.77159838982005
32-33	21.494684662006467	29.474354080835933	28.283387546247262	20.747573710910334
34-35	21.432852074066513	28.681114837622175	29.801912543646292	20.08412054466502
36-37	22.375224692863686	27.694402608258724	28.47241344340234	21.457959255475252
38-39	21.629840382970595	29.226680862432136	28.743684601948594	20.399794152648674
40-41	21.053833838656676	29.08786784180874	29.603031808786255	20.255266510748324
42-43	21.06621208978462	29.157405997369164	28.77666285081144	20.999719062034778
44-45	21.032597654628237	29.76922397804746	28.410679314997324	20.78749905232698
46-47	20.433805840091633	27.856406738718754	28.58932281324769	23.12046460794192
48-49	21.32914836412711	28.15759832172386	29.61542349429066	20.897829819858377
50-51	22.542474622875368	30.068590194021077	27.92487996962195	19.464055213481604
52-53	20.60128087753584	29.597749578218224	28.298755241195504	21.502214303050433
54-55	22.13961908638506	28.21097966097244	27.744340787037647	21.905060465604855
56-57	21.343994433120308	28.5613915886286	29.084517444093233	21.010096534157864
58-59	21.032380826711478	27.804504814559333	29.17976964363349	21.983344715095697
60-61	20.151903569917685	29.492312433519213	29.39862806303913	20.957155933523975
62-63	20.607066506617773	30.579711143913034	27.215582512656038	21.597639836813148
64-65	20.41454137413895	29.683745969513282	28.627897523854724	21.273815132493045
66-67	20.535032394827986	29.42537046220516	29.3008519703558	20.73874517261105
68-69	21.148451707403204	29.58848509174952	28.75607536355363	20.50698783729365
70-71	19.890795247817728	30.08157044271017	29.27608939731934	20.75154491215276
72-73	20.756402249209614	29.958331924814985	28.084482875753586	21.200782950221814
74-75	20.63470626402843	28.65548253869378	30.078354097902256	20.631457099375535
76-77	20.062311757676643	29.698464392273287	28.693586378714098	21.54563747133597
78-79	20.136960495080796	29.033485103236462	28.049201542401438	22.780352859281304
80-81	21.19550537606882	29.206407500798342	28.112847394868734	21.485239728264098
82-83	20.734806791213604	27.841172955003664	29.578081200891255	21.845939052891477
84-85	20.754367418820934	28.869402288265235	28.199614891939017	22.176615400974814
86-87	22.261750078849676	27.886874589136568	28.813329583119046	21.03804574889471
88-89	21.135898116698836	28.20092768573817	29.181420554478155	21.48175364308484
90-91	21.134946341194453	28.940998780086364	28.520428876606246	21.40362600211294
92-93	21.28659043491874	29.069078225115913	28.619676087821965	21.024655252143383
94-95	21.795035473329445	29.134012288406357	28.362827981018313	20.70812425724588
96-97	21.13819550584735	29.132436935847377	28.752022112698754	20.97734544560652
98-99	22.297359610651615	29.140362928409747	28.320178592468437	20.242098868470205
100	22.476933392452814	30.54106468233508	26.716043029442353	20.26595889576975
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	9.5
2	15.5
3	16.0
4	28.5
5	46.5
6	50.5
7	46.0
8	63.0
9	77.0
10	73.5
11	90.5
12	115.5
13	128.5
14	147.0
15	192.0
16	250.5
17	345.0
18	527.0
19	798.0
20	1216.0
21	1853.0
22	2864.0
23	4478.5
24	6958.0
25	10520.0
26	15099.5
27	20376.5
28	26177.0
29	33033.5
30	41476.0
31	51467.0
32	62125.0
33	73456.0
34	86131.0
35	99871.0
36	113530.0
37	124547.5
38	132622.0
39	138726.5
40	142272.5
41	143381.0
42	142645.0
43	140865.0
44	139225.0
45	136560.5
46	130691.0
47	121944.5
48	111817.0
49	105072.5
50	100767.0
51	93942.0
52	85121.5
53	75594.5
54	63966.5
55	50453.0
56	38927.5
57	29488.5
58	21853.5
59	17029.0
60	14377.5
61	12608.5
62	10843.5
63	9020.0
64	7442.0
65	7134.5
66	8625.5
67	8500.5
68	5648.0
69	4346.0
70	5177.0
71	4866.0
72	2887.5
73	1308.5
74	721.0
75	481.5
76	330.0
77	243.5
78	211.5
79	172.5
80	118.5
81	77.5
82	55.5
83	49.0
84	33.5
85	28.0
86	30.0
87	22.0
88	18.0
89	16.5
90	15.5
91	12.5
92	10.5
93	12.5
94	17.0
95	14.5
96	16.0
97	24.0
98	41.0
99	87.0
100	92.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.344671714577622
2	0.006498329305791357
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	3.774282172555586E-4
30-31	0.010535170238176897
32-33	0.0
34-35	0.0
36-37	0.0
38-39	6.235770545961404E-4
40-41	0.0014112533340860018
42-43	3.281984497874423E-5
44-45	0.0
46-47	0.002198929613575863
48-49	3.281984497874423E-5
50-51	0.0
52-53	0.0013620235666178854
54-55	0.01893705055273542
56-57	0.011388486207624247
58-59	0.013013068534072086
60-61	1.312793799149769E-4
62-63	0.012077702952177876
64-65	1.148694574256048E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	2.625587598299538E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3046937.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.75726614260263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.98193984583503	65.02532026654941
2	8.096612850696502	12.1056128346627
3	2.251935467552447	5.050456172513536
4	0.9651070869191132	2.8859506941169624
5	0.49054754244029525	1.8335996592904407
6	0.29496639760649257	1.3230528893395983
7	0.19372523610666847	1.0137658323915322
8	0.13523399872076686	0.8087779227117401
9	0.09736625826464597	0.6550951754159569
>10	0.4556442633521502	5.973601928837067
>50	0.024324414330792188	1.2369869014707808
>100	0.011792764292032075	1.5775028870951786
>500	6.25235242490171E-4	0.3097250854949403
>1k	1.786386403710149E-4	0.20055175011020704
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	1.6409922489372113E-5	0.0
40-41	0.0	0.0	0.0	3.281984497874423E-5	0.0
42-43	0.0	0.0	0.0	3.281984497874423E-5	0.0
44-45	0.0	0.0	0.0	3.281984497874423E-5	0.0
46-47	0.0	0.0	0.0	3.281984497874423E-5	0.0
48-49	0.0	0.0	0.0	3.281984497874423E-5	0.0
50-51	3.281984497874423E-5	0.0	0.0	3.281984497874423E-5	0.0
52-53	6.563968995748845E-5	0.0	0.0	3.281984497874423E-5	0.0
54-55	1.312793799149769E-4	0.0	0.0	3.281984497874423E-5	0.0
56-57	1.4768930240434901E-4	0.0	0.0	3.281984497874423E-5	0.0
58-59	1.6409922489372115E-4	0.0	0.0	3.281984497874423E-5	0.0
60-61	1.6409922489372115E-4	0.0	0.0	3.281984497874423E-5	0.0
62-63	2.789686823193259E-4	0.0	0.0	3.281984497874423E-5	0.0
64-65	3.446083722768144E-4	0.0	0.0	3.281984497874423E-5	0.0
66-67	3.938381397449307E-4	0.0	0.0	3.281984497874423E-5	0.0
68-69	4.758877521917913E-4	0.0	0.0	3.281984497874423E-5	0.0
70-71	5.251175196599076E-4	0.0	0.0	3.281984497874423E-5	0.0
72-73	6.235770545961403E-4	0.0	0.0	3.281984497874423E-5	0.0
74-75	7.056266670430009E-4	0.0	0.0	3.281984497874423E-5	0.0
76-77	8.204961244686057E-4	0.0	0.0	3.281984497874423E-5	0.0
78-79	0.0011158747292773037	0.0	0.0	3.281984497874423E-5	0.0
80-81	0.0015753525589797229	0.0	0.0	3.281984497874423E-5	0.0
82-83	0.0017886815513415604	0.0	0.0	3.281984497874423E-5	1.312793799149769E-4
84-85	0.0019527807762352817	0.0	0.0	3.281984497874423E-5	1.312793799149769E-4
86-87	0.0020020105437033977	0.0	0.0	3.281984497874423E-5	1.312793799149769E-4
88	0.0020020105437033977	0.0	0.0	3.281984497874423E-5	1.312793799149769E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	9825	0.0	77.96661	8
CTTCACC	11345	0.0	70.58226	6
CACCGGC	10410	0.0	70.56424	9
CCTTCAC	12455	0.0	68.24892	5
TTCACCG	12265	0.0	64.02497	7
GTGTGGC	4520	0.0	62.72195	7
GCTGCCT	130	0.0	61.082123	1
AGGCGCG	1115	0.0	61.039948	5
CGGGGCA	1425	0.0	60.27778	94
CAGCGTA	1155	0.0	60.042057	1
CGGGCCC	815	0.0	59.895855	7
CGTAGTG	2020	0.0	59.25293	4
GGGCCCG	835	0.0	59.02335	8
GCGTAGT	1345	0.0	58.97738	3
CGAGCGA	1000	0.0	58.202644	7
GCGCGCA	1210	0.0	56.63547	7
ACCTTCA	5025	0.0	56.23173	4
GCAGCCA	85	1.8189894E-12	55.223446	2
CAGCAGC	125	0.0	55.055355	1
TGTGTGG	4935	0.0	53.452766	6
>>END_MODULE