FastQCFastQC Report
Tue 7 Mar 2017
1NF_22A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_22A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2895176
Sequences flagged as poor quality0
Sequence length95
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA165010.5699480791495922No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT71000.24523552281450248No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG67180.2320411608827926No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA66900.23107403487732697No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA62800.21691254694015147No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG55570.19193997187044934No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG51210.17688043835677003No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG50140.17318463540731202No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT47790.1650676850042968No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA44000.15197694371602968No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT43060.1487301635548236No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC42100.14541430296465568No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA40850.14109677615454122No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG40530.1399914892911519No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC40160.138713501355358No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC38650.13349792896873972No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA37910.13094195309715195No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG31860.11004512333619787No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA31730.10959610054794597No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA30550.1055203552391979No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC30130.10406966623099942No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA30070.10386242494411393No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA29590.10220449464902998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTT450.088.999883
AGCTGCG800.088.999882
GCAGCAA850.088.999883
AGCAGCG1050.088.999882
GCAGCTA158.578022E-488.999873
CAGCTGC4700.088.053061
AGCTGCC1850.086.594482
GCTGCCC1100.084.954433
CAGCAGC4300.084.860351
CAGCCCT178650.083.021721
CAGCGTA56050.082.410231
AGCAGCC1050.080.52372
AGCTGCT1550.080.3869862
AGCCCTT153850.080.2936862
GCCCTTC140250.079.29083
GCTGCAC454.5474735E-1179.1113
GCAGCAT454.5474735E-1179.1113
CCCTTCA137050.078.73944
AGCAGCA1700.078.5293052
CAGCTCT65300.078.368961
Produced by FastQC (version 0.11.5)