FastQCFastQC Report
Tue 7 Mar 2017
1NF_22A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_22A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2895176
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGCC307.1564864E-788.27441
TCACCGG109850.079.86178
CACCGGC106250.077.66399
CTTCACC123900.074.063596
GTGTGGC46600.070.6092157
CCTTCAC137900.070.52695
ATGCAGC800.070.412632
CGTAGTG22600.065.838534
CGGGGCA13650.065.33565594
TTCACCG139350.064.908877
CGGGCCC7450.063.6347547
ACGCAGC301.3799511E-462.5892
TGTGGCA54950.061.5881048
GCCTTCA40550.061.5814744
GGGCCCG7750.061.1714748
GCGTAGT14550.060.6490673
ACCTTCA54350.060.368024
TGTGTGG53400.058.980796
GCAGCCA801.8189894E-1258.6771932
GGCCCGA8200.058.386939
Produced by FastQC (version 0.11.5)