##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1NF_22A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2895176
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.784096027322692	12.0	12.0	12.0	2.0	27.0
2	14.556490520783537	12.0	12.0	12.0	12.0	22.0
3	13.716646932690793	12.0	12.0	12.0	12.0	22.0
4	13.757663437386881	12.0	12.0	12.0	12.0	22.0
5	22.432381312915002	22.0	22.0	22.0	22.0	27.0
6	26.806924000475274	27.0	22.0	32.0	22.0	32.0
7	34.94938753291682	37.0	37.0	37.0	27.0	41.0
8	37.5738100205307	41.0	37.0	41.0	27.0	41.0
9	38.530485193300855	41.0	37.0	41.0	32.0	41.0
10-11	38.89318628642956	41.0	41.0	41.0	37.0	41.0
12-13	39.075013401603215	41.0	41.0	41.0	37.0	41.0
14-15	39.02750989922547	41.0	41.0	41.0	37.0	41.0
16-17	38.93129398696314	41.0	41.0	41.0	37.0	41.0
18-19	38.85870012738431	41.0	41.0	41.0	37.0	41.0
20-21	38.87732196591848	41.0	41.0	41.0	37.0	41.0
22-23	38.86427301828974	41.0	41.0	41.0	37.0	41.0
24-25	38.87419175898114	41.0	41.0	41.0	37.0	41.0
26-27	38.834864443474245	41.0	41.0	41.0	37.0	41.0
28-29	38.786745261773376	41.0	41.0	41.0	34.5	41.0
30-31	38.65935974186026	41.0	41.0	41.0	32.0	41.0
32-33	38.70557316722714	41.0	41.0	41.0	32.0	41.0
34-35	38.68302117038826	41.0	41.0	41.0	32.0	41.0
36-37	38.607615219247464	41.0	41.0	41.0	32.0	41.0
38-39	38.508754735463405	41.0	41.0	41.0	32.0	41.0
40-41	38.55645511706369	41.0	41.0	41.0	32.0	41.0
42-43	38.29376107013874	41.0	41.0	41.0	32.0	41.0
44-45	38.41024414405204	41.0	41.0	41.0	32.0	41.0
46-47	38.41921717367096	41.0	41.0	41.0	32.0	41.0
48-49	38.374025447848425	41.0	41.0	41.0	32.0	41.0
50-51	38.268145528976476	41.0	41.0	41.0	32.0	41.0
52-53	38.23205204105035	41.0	41.0	41.0	32.0	41.0
54-55	38.09554116917245	41.0	37.0	41.0	32.0	41.0
56-57	37.8795579267029	41.0	37.0	41.0	29.5	41.0
58-59	37.88327773510142	41.0	37.0	41.0	29.5	41.0
60-61	37.88045683578477	41.0	37.0	41.0	29.5	41.0
62-63	37.878197214953424	41.0	37.0	41.0	29.5	41.0
64-65	37.85943583395276	41.0	37.0	41.0	29.5	41.0
66-67	37.7272528509493	41.0	37.0	41.0	27.0	41.0
68-69	37.75352586509421	41.0	37.0	41.0	27.0	41.0
70-71	37.7284156472698	41.0	37.0	41.0	27.0	41.0
72-73	37.57963350069218	41.0	37.0	41.0	27.0	41.0
74-75	37.49658449089105	41.0	37.0	41.0	27.0	41.0
76-77	36.45973560847423	39.0	34.5	41.0	24.5	41.0
78-79	36.96579258048561	41.0	37.0	41.0	27.0	41.0
80-81	37.6292037168034	41.0	37.0	41.0	27.0	41.0
82-83	37.811345493330975	41.0	37.0	41.0	27.0	41.0
84-85	37.73108025211593	41.0	37.0	41.0	27.0	41.0
86-87	37.6016589319613	41.0	37.0	41.0	27.0	41.0
88-89	37.5100208070252	41.0	37.0	41.0	27.0	41.0
90-91	37.34424383871654	41.0	37.0	41.0	27.0	41.0
92-93	37.23337078643924	41.0	37.0	41.0	27.0	41.0
94-95	37.07813877291052	41.0	37.0	41.0	27.0	41.0
96-97	37.014855228145024	41.0	37.0	41.0	27.0	41.0
98-99	36.89773005164453	41.0	37.0	41.0	27.0	41.0
100	36.75819052105986	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.6751754448718827
1101	2	-0.46569582818050215
1101	3	0.06255565058732593
1101	4	0.05607898131045275
1101	5	-1.1495818565441773
1101	6	-0.6670062384202922
1101	7	-0.2577332080672363
1101	8	-0.11552057457427622
1101	9	-0.21174859188293738
1101	10-11	-0.008464246748971505
1101	12-13	0.07539811327688994
1101	14-15	0.05129589230278242
1101	16-17	0.07121708579074948
1101	18-19	0.06184036629248624
1101	20-21	0.1109622419693892
1101	22-23	0.07461223742783574
1101	24-25	-0.026612314711925933
1101	26-27	0.0757404516645579
1101	28-29	0.06321228312362592
1101	30-31	-0.12067052467926942
1101	32-33	-0.06751921524044491
1101	34-35	0.09042878994974046
1101	36-37	0.08672516829106058
1101	38-39	0.08249572233466296
1101	40-41	0.20688544057844638
1101	42-43	0.24158809198178943
1101	44-45	0.15844998022021173
1101	46-47	0.044966259086265836
1101	48-49	0.1733117474605237
1101	50-51	0.08502228544677592
1101	52-53	0.08838357006947462
1101	54-55	-0.06150412045726483
1101	56-57	0.0762360514414624
1101	58-59	0.1504227736968602
1101	60-61	0.060430418783262496
1101	62-63	-0.10416646493708726
1101	64-65	-0.08564523036596228
1101	66-67	-0.10699245200172669
1101	68-69	-0.06181114874324578
1101	70-71	-0.12031980904303197
1101	72-73	0.0946832975604508
1101	74-75	0.003579637326843965
1101	76-77	-0.2517803370120646
1101	78-79	-0.09182780224347198
1101	80-81	-0.20023047800153648
1101	82-83	-0.07465272390330568
1101	84-85	-0.08550173282131368
1101	86-87	-0.11564481780270341
1101	88-89	-0.2475149751109882
1101	90-91	-0.2035184630831779
1101	92-93	0.04924553380038077
1101	94-95	0.061947863150372484
1101	96-97	0.07532709104870605
1101	98-99	-0.2579898500494835
1101	100	-0.3794983966795371
1102	1	1.6908599198166936
1102	2	0.7609707769934921
1102	3	-0.0314681839727875
1102	4	-0.15783404345264707
1102	5	-0.7417755154197216
1102	6	-0.17992431173185608
1102	7	-0.01749301768592204
1102	8	-0.0977396606701717
1102	9	-0.03932313637596252
1102	10-11	-0.15208276772720097
1102	12-13	-0.14363878847626665
1102	14-15	-0.12141980862145374
1102	16-17	-0.2267063835028864
1102	18-19	-0.06887899037117506
1102	20-21	-0.0263620427170963
1102	22-23	0.013417342549566058
1102	24-25	0.0356227287326476
1102	26-27	-0.04323841637831549
1102	28-29	-0.11001403758526607
1102	30-31	-0.04199964268985923
1102	32-33	-0.011311837349524012
1102	34-35	0.031391596106381314
1102	36-37	-0.20518301084939594
1102	38-39	0.01243073995230759
1102	40-41	8.462451485868883E-4
1102	42-43	0.020416164616939625
1102	44-45	-0.014260263229282089
1102	46-47	-0.20617904195111691
1102	48-49	-0.00709791672797877
1102	50-51	-0.01195950042344407
1102	52-53	-0.07605421779310717
1102	54-55	-0.038537375694907894
1102	56-57	-0.13323697172282323
1102	58-59	0.06060903500382864
1102	60-61	-0.1344328676311619
1102	62-63	-0.1678319816907603
1102	64-65	-0.1090386574141391
1102	66-67	-0.012103876725468865
1102	68-69	-0.15148130204448051
1102	70-71	-0.4090416684584923
1102	72-73	-0.04086192722601112
1102	74-75	-0.1191067008459683
1102	76-77	0.10761758822514622
1102	78-79	0.027183118855283794
1102	80-81	-0.11584162293362255
1102	82-83	-0.17791783111578496
1102	84-85	-0.1915287834482342
1102	86-87	-0.2774750088604421
1102	88-89	-0.31430508831900283
1102	90-91	-0.2890819050740632
1102	92-93	-0.09736898707945585
1102	94-95	0.04004163640229308
1102	96-97	0.09752994307889651
1102	98-99	-0.07663212257271113
1102	100	-0.4470296503306699
1103	1	1.4329367701250941
1103	2	0.6633598487654577
1103	3	0.3484618711972942
1103	4	1.0262994404254577
1103	5	2.856763443761537
1103	6	-0.08849728970832871
1103	7	-0.30019486402090223
1103	8	-0.06395958827135928
1103	9	-0.26984863171784923
1103	10-11	-0.26715709602923
1103	12-13	-0.5009423078417399
1103	14-15	-0.43652311107022257
1103	16-17	-0.3435746294910089
1103	18-19	-0.29678672003142026
1103	20-21	-0.25854071736364403
1103	22-23	-0.2685541899936652
1103	24-25	-0.28642054144563645
1103	26-27	-0.20052265673117375
1103	28-29	-0.2235514487237893
1103	30-31	-0.24849320512079487
1103	32-33	-0.38525093537781885
1103	34-35	-0.4891809222253656
1103	36-37	-0.2572608211350129
1103	38-39	-0.12038441230261299
1103	40-41	-0.43270362344261315
1103	42-43	-0.28190672594438837
1103	44-45	-0.5383203279228468
1103	46-47	-0.3932118477420019
1103	48-49	-0.3985181362639665
1103	50-51	-0.3133772469055387
1103	52-53	-0.2562451992204018
1103	54-55	-0.4209953178852075
1103	56-57	-0.2649956722315494
1103	58-59	-0.45152418694672036
1103	60-61	-0.37320391560840704
1103	62-63	-0.28119162221426564
1103	64-65	-0.4477261683012941
1103	66-67	-0.43021384688353237
1103	68-69	-0.38034781689488284
1103	70-71	-0.5296053499138935
1103	72-73	-0.4505693948315539
1103	74-75	-0.37225094409783566
1103	76-77	-0.5798198702129298
1103	78-79	-0.5074808001150046
1103	80-81	-0.32871243797334415
1103	82-83	-0.43834709350998224
1103	84-85	-0.26731532901790445
1103	86-87	-0.3124052261458843
1103	88-89	-0.252725569782541
1103	90-91	-0.5289651915528779
1103	92-93	-0.3442611403147069
1103	94-95	-0.3893100007085124
1103	96-97	-0.28433405294387626
1103	98-99	-0.1715876966072969
1103	100	-0.3210094361349576
1104	1	1.455596120706268
1104	2	-0.5483526738106423
1104	3	0.08524552674563424
1104	4	0.06706459464664327
1104	5	-1.2447816426206089
1104	6	-0.43089797607828473
1104	7	-0.2591744573322572
1104	8	-0.30197866576224186
1104	9	-0.06339305334402923
1104	10-11	-0.11870940801968999
1104	12-13	-0.09652548197608013
1104	14-15	-0.2663525741478381
1104	16-17	-0.08642080602895419
1104	18-19	-0.0324361906927777
1104	20-21	-0.08408500201743152
1104	22-23	-0.08787479117683716
1104	24-25	-0.22745258764680187
1104	26-27	-0.21221973849647924
1104	28-29	-0.10956700640095818
1104	30-31	-0.2424756871046867
1104	32-33	-0.1811744670792379
1104	34-35	-0.3083574824776676
1104	36-37	-0.17816075180058277
1104	38-39	-0.2035904335590999
1104	40-41	-0.21683166373512108
1104	42-43	-0.29973622860577365
1104	44-45	-0.265972935126527
1104	46-47	-0.3916934489870698
1104	48-49	-0.3643153266332959
1104	50-51	-0.21305407371996665
1104	52-53	-0.1925311203111093
1104	54-55	-0.28989183869197177
1104	56-57	-0.10282671112008757
1104	58-59	-0.2615189625177621
1104	60-61	-0.1786351697572428
1104	62-63	-0.34803918041418314
1104	64-65	-0.3996677835012079
1104	66-67	-0.7260915116800248
1104	68-69	-0.5749416952966939
1104	70-71	-0.39374687067824965
1104	72-73	-0.17370379774340705
1104	74-75	-0.3099482935247906
1104	76-77	-0.4428254782274479
1104	78-79	-0.29579898637422275
1104	80-81	-0.3307571280465922
1104	82-83	-0.32980793244080786
1104	84-85	-0.24670278906893373
1104	86-87	-0.16688650129610494
1104	88-89	-0.41363452617536467
1104	90-91	-0.334911898607551
1104	92-93	-0.30978886261761573
1104	94-95	-0.2578635407382279
1104	96-97	-0.40833601579608114
1104	98-99	-0.571955275868298
1104	100	-0.5246154545011947
1105	1	1.6094352346608538
1105	2	0.2206641013651538
1105	3	-0.16390019873010964
1105	4	-0.26766768014186404
1105	5	-1.1243013579068801
1105	6	0.4262972639852798
1105	7	-0.05667866658132681
1105	8	0.0430485413206938
1105	9	0.024162054787304044
1105	10-11	-0.06181711873367135
1105	12-13	-0.06402320007215678
1105	14-15	-0.03622422993125696
1105	16-17	-0.11812654285058244
1105	18-19	-0.2124191658106298
1105	20-21	-0.02762197128537025
1105	22-23	-0.04575790557265691
1105	24-25	-0.10631409638227751
1105	26-27	-0.010317454895520939
1105	28-29	-0.11464992466819268
1105	30-31	-0.1291714296113753
1105	32-33	-0.11222202765423361
1105	34-35	-0.14907316391539638
1105	36-37	-0.21960488485285623
1105	38-39	-0.23347610199027713
1105	40-41	-0.10213735400954249
1105	42-43	-0.09619085296134955
1105	44-45	0.04492571682281721
1105	46-47	-0.2562707846202841
1105	48-49	-0.20668728242799972
1105	50-51	-0.2200117594026949
1105	52-53	-0.23948955147640305
1105	54-55	-0.31337044819123605
1105	56-57	-0.3401035057632811
1105	58-59	-0.21575042454845317
1105	60-61	-0.25200974504431173
1105	62-63	-0.39652327496803963
1105	64-65	-0.3737374152085806
1105	66-67	-0.30440975757775135
1105	68-69	-0.35568785060676333
1105	70-71	-0.3255285497473963
1105	72-73	-0.28003249009427833
1105	74-75	-0.1871624676331507
1105	76-77	-0.22750072056988557
1105	78-79	-0.20233729266209366
1105	80-81	-0.3845305122585998
1105	82-83	-0.19677475180007775
1105	84-85	-0.23052248411202214
1105	86-87	-0.33586375983551164
1105	88-89	-0.3230746081663227
1105	90-91	-0.28753436078491745
1105	92-93	-0.2856984119461359
1105	94-95	-0.17189834083374933
1105	96-97	-0.09265763879039923
1105	98-99	-0.32873128805784546
1105	100	-0.5721860298803918
1106	1	1.3874834279300412
1106	2	-0.5928724266134271
1106	3	-0.19450281522798107
1106	4	-0.25170975471168866
1106	5	-1.2570961030239012
1106	6	-0.8870909171486474
1106	7	-0.3621555226496298
1106	8	-0.4411029694460069
1106	9	-0.455596200653801
1106	10-11	-0.3004018594367608
1106	12-13	-0.2255824895637204
1106	14-15	-0.2358027181796416
1106	16-17	-0.17526774107966503
1106	18-19	-0.1633763981448908
1106	20-21	-0.27119624259228914
1106	22-23	-0.12316258360528565
1106	24-25	-0.36770184463524913
1106	26-27	-0.3533678612077935
1106	28-29	-0.25247903883080625
1106	30-31	-0.16919893457551893
1106	32-33	-0.163893087981549
1106	34-35	-0.17735892492863314
1106	36-37	-0.542328429564229
1106	38-39	-0.30660532060367274
1106	40-41	-0.3119548144331574
1106	42-43	-0.14454660450227408
1106	44-45	-0.22603617870938564
1106	46-47	-0.46371955936047016
1106	48-49	-0.5681434891019066
1106	50-51	-0.65284027774603
1106	52-53	-0.5665070989625889
1106	54-55	-0.6284313311954364
1106	56-57	-0.5035820325575173
1106	58-59	-0.6389449096936985
1106	60-61	-0.4624014415120783
1106	62-63	-0.5207256066886075
1106	64-65	-0.808095152408498
1106	66-67	-0.6455939727439031
1106	68-69	-0.5368498988632666
1106	70-71	-0.8596486280064397
1106	72-73	-0.7453210003879676
1106	74-75	-0.9909126637063395
1106	76-77	-0.8770528833331284
1106	78-79	-0.6214484397115143
1106	80-81	-0.5870484263560556
1106	82-83	-0.6305175183714553
1106	84-85	-0.8579828226103317
1106	86-87	-0.6231103694413349
1106	88-89	-0.75246439801235
1106	90-91	-0.748049780953032
1106	92-93	-0.8241024613286072
1106	94-95	-0.5313076346419692
1106	96-97	-0.5958782548675501
1106	98-99	-0.9227877474077673
1106	100	-0.8730118475815729
1107	1	1.3214840959989171
1107	2	-0.5874183661372872
1107	3	-0.1776634417444427
1107	4	-0.21923469411441587
1107	5	-1.246004446665804
1107	6	-0.41766516272636167
1107	7	-0.8073090255452016
1107	8	-0.492647664599815
1107	9	-0.5036589232669542
1107	10-11	-0.3245824962067374
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2223	2	-0.9423666080864326
2223	3	-0.17087961954519315
2223	4	0.03224231267658517
2223	5	-1.0839913384548687
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2223	34-35	0.14449441399967355
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2223	48-49	0.22640628937086404
2223	50-51	0.19451738532695373
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2223	54-55	0.2673616538153212
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2223	60-61	0.05027854189430059
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2223	66-67	0.27985099453510287
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2223	78-79	0.5102773267870617
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2223	94-95	0.4014206531784552
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2223	100	0.5070119668950426
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2224	3	-0.03516089489041363
2224	4	-0.350321315898956
2224	5	-1.1077017384661012
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2224	7	0.2579515261563827
2224	8	0.09547438316066348
2224	9	0.10537921935327432
2224	10-11	0.1323026788569166
2224	12-13	0.17012242245523623
2224	14-15	0.08722152728576305
2224	16-17	0.10434228301649284
2224	18-19	-0.06554385520014705
2224	20-21	-0.023204978607886062
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2224	24-25	0.1514991522447815
2224	26-27	0.07880945214125745
2224	28-29	0.014272546359855198
2224	30-31	0.0035169052426482494
2224	32-33	0.1589675591646298
2224	34-35	0.21070907990795007
2224	36-37	0.1072115801364717
2224	38-39	0.0699968133707074
2224	40-41	0.2392664952690069
2224	42-43	0.3197228343741969
2224	44-45	0.27122394216934964
2224	46-47	0.23401986644309147
2224	48-49	0.1427141730294892
2224	50-51	0.11469354229792117
2224	52-53	0.25057240099357614
2224	54-55	0.05672837876502257
2224	56-57	0.21523247731926887
2224	58-59	0.2049425723643381
2224	60-61	0.2099786739088927
2224	62-63	0.3761953338064856
2224	64-65	0.2622193603108798
2224	66-67	0.21095895157418454
2224	68-69	0.23831776136210436
2224	70-71	0.3232453162974238
2224	72-73	0.3060488718482546
2224	74-75	0.10055315928072872
2224	76-77	0.356276320769652
2224	78-79	0.29767743808659475
2224	80-81	0.28476514666415653
2224	82-83	0.1415903969745287
2224	84-85	0.15509846009623374
2224	86-87	0.22100436990697148
2224	88-89	0.38160371394485537
2224	90-91	0.3005126633360149
2224	92-93	0.30927288243731255
2224	94-95	0.6279520751090288
2224	96-97	0.4215507635096216
2224	98-99	0.36619937029056615
2224	100	0.6619184507074536
2225	1	1.4053139282844924
2225	2	1.6242410842578447
2225	3	0.7983859599013048
2225	4	0.9356760212578248
2225	5	3.1914062626218893
2225	6	-0.12541346696339772
2225	7	0.2815794982709292
2225	8	0.3369832225026528
2225	9	0.2166192865135912
2225	10-11	0.2643881689801688
2225	12-13	0.11774196322489416
2225	14-15	0.15487219308584343
2225	16-17	0.2385997401976283
2225	18-19	0.23431489328734756
2225	20-21	0.15880882187170897
2225	22-23	0.2069637118456953
2225	24-25	0.3293390917981256
2225	26-27	0.2641771018696417
2225	28-29	0.1899768249430096
2225	30-31	0.19878346929998258
2225	32-33	0.3001030112260068
2225	34-35	0.24731097294307602
2225	36-37	0.28408873878633045
2225	38-39	0.0894243645727073
2225	40-41	0.11967446558610106
2225	42-43	0.4041979495269814
2225	44-45	0.3913487339301298
2225	46-47	0.45523776903445423
2225	48-49	0.28125984043358443
2225	50-51	0.3834097024946601
2225	52-53	0.3913424858486394
2225	54-55	0.39736050048683325
2225	56-57	0.4163117352942578
2225	58-59	0.3999183157314903
2225	60-61	0.31952991280949306
2225	62-63	0.362807984752024
2225	64-65	0.5694974214943684
2225	66-67	0.6685684694829064
2225	68-69	0.6266216361739438
2225	70-71	0.45868606922616095
2225	72-73	0.44158492825720685
2225	74-75	0.4010956972190982
2225	76-77	0.5261929030693011
2225	78-79	0.4213018986287267
2225	80-81	0.32756124512864204
2225	82-83	0.2859235374689746
2225	84-85	0.5371680291072707
2225	86-87	0.3995561468518929
2225	88-89	0.298037366950318
2225	90-91	0.4109493095105705
2225	92-93	0.4538039208687934
2225	94-95	0.6496413170200626
2225	96-97	0.6950562025781863
2225	98-99	0.7264786652858604
2225	100	0.6832572049563126
2226	1	1.4641567408646523
2226	2	1.308426135706533
2226	3	0.8510985187149682
2226	4	1.0363969426883308
2226	5	3.151004287786275
2226	6	-0.3426168335355335
2226	7	0.10899618750936213
2226	8	0.18503589156222944
2226	9	0.05218498292297369
2226	10-11	0.03324778033912423
2226	12-13	0.18204400520778563
2226	14-15	-0.10560327603328545
2226	16-17	-0.07217310826416679
2226	18-19	-0.06787559990135605
2226	20-21	0.1429944562999097
2226	22-23	0.09733359291190169
2226	24-25	0.1159016981171419
2226	26-27	0.13010011090492668
2226	28-29	0.08525778519098282
2226	30-31	0.033357278392429635
2226	32-33	0.036280268089178946
2226	34-35	-0.11460031491545664
2226	36-37	-0.037881427013040536
2226	38-39	-0.001508405632314691
2226	40-41	0.1569849299786128
2226	42-43	0.25797585107579124
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2226	46-47	0.008489060707773888
2226	48-49	0.08417733896716584
2226	50-51	0.02058485584817049
2226	52-53	0.15901969517655345
2226	54-55	0.11148939755537413
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2226	58-59	0.1035062705975136
2226	60-61	0.2223209197376832
2226	62-63	0.22205961545035535
2226	64-65	0.25878100245946456
2226	66-67	0.19620065186520463
2226	68-69	0.22925099334785415
2226	70-71	0.30409455250481443
2226	72-73	0.15330447564985406
2226	74-75	0.21909054069059408
2226	76-77	0.3037806145787556
2226	78-79	0.25167773206520394
2226	80-81	0.13251468748811845
2226	82-83	0.09193030421678117
2226	84-85	0.2964249215693755
2226	86-87	0.19442755652026023
2226	88-89	0.1965605154223482
2226	90-91	0.3014411678280098
2226	92-93	0.3796333063844699
2226	94-95	0.3966659540331037
2226	96-97	0.23454925256554304
2226	98-99	0.30588167035090663
2226	100	0.4546679495975354
2227	1	1.1441793253460144
2227	2	1.6293848687308579
2227	3	0.5906784939275607
2227	4	-0.0817005520640457
2227	5	3.2187763881012508
2227	6	0.15146884421382723
2227	7	0.21948701640699397
2227	8	0.2874429029601373
2227	9	0.31600118715402203
2227	10-11	0.2948044170603126
2227	12-13	0.2284589053536905
2227	14-15	0.12429837132642518
2227	16-17	0.20631524786259092
2227	18-19	0.03095911727621825
2227	20-21	0.12652765005482536
2227	22-23	0.22198845272453838
2227	24-25	0.23836534683323407
2227	26-27	0.08021858540470106
2227	28-29	0.037448220188508685
2227	30-31	0.1916072258564938
2227	32-33	0.09725829314404422
2227	34-35	0.1736425610925707
2227	36-37	0.15517626281481256
2227	38-39	0.20261690391178888
2227	40-41	0.3142849023972474
2227	42-43	0.4807494767238367
2227	44-45	0.1552429893129883
2227	46-47	0.2529798770684195
2227	48-49	0.09661850469176869
2227	50-51	0.3526840424963993
2227	52-53	0.3956048236866039
2227	54-55	0.3496093364942894
2227	56-57	0.48608638682097194
2227	58-59	0.38141367183592934
2227	60-61	0.292431466087514
2227	62-63	0.34690058977699323
2227	64-65	0.45274739976007083
2227	66-67	0.3636247613214394
2227	68-69	0.35623123983690164
2227	70-71	0.4952655008500457
2227	72-73	0.4871545118280025
2227	74-75	0.43675534531662663
2227	76-77	0.5335689813321096
2227	78-79	0.5753730349945556
2227	80-81	0.39012463683869925
2227	82-83	0.297237448387321
2227	84-85	0.3822540848629927
2227	86-87	0.2745540337948853
2227	88-89	0.35540022351359823
2227	90-91	0.46856573291406534
2227	92-93	0.5375588593848732
2227	94-95	0.44451902535540455
2227	96-97	0.405732815504777
2227	98-99	0.6249728404932995
2227	100	0.5715598043786585
2228	1	1.4187479698871144
2228	2	1.5999367667794981
2228	3	-0.12316296019940509
2228	4	-0.11022840082942587
2228	5	3.187852707765476
2228	6	0.3284843044673522
2228	7	0.5002298193924233
2228	8	0.587163019060128
2228	9	0.300404482295086
2228	10-11	0.18477968657813193
2228	12-13	0.25557569923390844
2228	14-15	0.30515192202285846
2228	16-17	0.27304841251810075
2228	18-19	0.21865057224939477
2228	20-21	0.21393424964525565
2228	22-23	0.3016395392579554
2228	24-25	0.3422376029978764
2228	26-27	0.38554061055406663
2228	28-29	0.2627425954317957
2228	30-31	0.28641075709009556
2228	32-33	0.2613801600521626
2228	34-35	0.40372489730866334
2228	36-37	0.37797114578399515
2228	38-39	0.3544348799471351
2228	40-41	0.22438938815553655
2228	42-43	0.5346387628612277
2228	44-45	0.40415772521090787
2228	46-47	0.3157427309956802
2228	48-49	0.46283907774506616
2228	50-51	0.33727773484835666
2228	52-53	0.3903265898957571
2228	54-55	0.3995034544897962
2228	56-57	0.4665045625618234
2228	58-59	0.5528478494045928
2228	60-61	0.37854283576273673
2228	62-63	0.33740241335917176
2228	64-65	0.26833395010109484
2228	66-67	0.3805775174268362
2228	68-69	0.49296817012614014
2228	70-71	0.5885336901302693
2228	72-73	0.656487444622023
2228	74-75	0.6473901930834742
2228	76-77	0.6458890559678139
2228	78-79	0.5379658007467469
2228	80-81	0.47912898635720325
2228	82-83	0.48902455891924745
2228	84-85	0.3177416921200873
2228	86-87	0.5605153070021913
2228	88-89	0.520468258783616
2228	90-91	0.5179209210265086
2228	92-93	0.5281754501747287
2228	94-95	0.7042038968161322
2228	96-97	0.510586675067799
2228	98-99	0.7020953462898376
2228	100	0.6296563399956341
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	19.0
13	41.0
14	120.0
15	443.0
16	1813.0
17	4717.0
18	7704.0
19	9962.0
20	11659.0
21	13536.0
22	15782.0
23	18021.0
24	20457.0
25	22778.0
26	26107.0
27	29452.0
28	33461.0
29	37906.0
30	43180.0
31	49817.0
32	58607.0
33	70156.0
34	85757.0
35	110661.0
36	155426.0
37	258182.0
38	634677.0
39	1173491.0
40	1244.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.167839705517302	20.475837711851362	18.877748451265273	34.47857413136607
2	19.5733633251077	51.03346579086264	12.065701882131222	17.327469001898436
3	39.788703691934444	15.708267822059868	23.752614694236204	20.750413791769482
4	19.235307283564108	16.226405579488087	22.096238708803888	42.44204842814392
5	17.73177174720984	35.295470810755546	26.205695266885332	20.767062175149285
6	17.5499520581823	27.900479970820424	30.813325338425024	23.736242632572253
7	20.47840960273227	26.321163203895033	36.4176823792405	16.782744814132197
8	20.628797696582176	27.936090931950254	34.14341649695908	17.291694874508494
9	21.02300516445287	28.1589789359956	27.36918239167498	23.44883350787655
10-11	19.44893851012857	30.362368298162185	28.629226686046028	21.559466505663213
12-13	24.13642555754814	26.52077110338024	27.407107547175023	21.93569579189659
14-15	21.781145602201732	27.39418950695916	28.564170192071224	22.260494698767882
16-17	20.985978054529326	29.553229233732246	28.256917714156238	21.203874997582183
18-19	21.435916158464977	29.585127121805378	29.64835298441269	19.330603735316952
20-21	22.85342929065452	28.918483712216457	29.791781225044694	18.43630577208432
22-23	22.013100412548322	30.150135950284195	28.725628424662265	19.111135212505214
24-25	22.33166481070581	28.24125372688914	26.551442813839298	22.87563864856575
26-27	20.241791863430755	26.303150482043236	30.069743601079868	23.385314053446145
28-29	19.596412284996585	28.043621115604246	30.685014545840772	21.6749520535584
30-31	20.123634475679594	29.938656007477967	28.23815086128057	21.69955865556187
32-33	20.65264771468125	30.100138989823073	28.796038651881613	20.451174643614067
34-35	21.376196127627477	29.17762167135953	29.798343865796067	19.647838335216928
36-37	22.04461835826216	27.97936982069484	28.664267733636915	21.311744087406083
38-39	21.552523780715372	29.214457072266192	28.871676964634478	20.361342182383957
40-41	20.864054463875544	29.77191006966872	29.740098026686194	19.623937439769545
42-43	20.629409275777803	29.85342854922414	29.37932940150423	20.13783277349383
44-45	20.721434551819993	29.983565765949983	28.77149782949292	20.523501852737105
46-47	20.005084424063764	28.03602203918219	28.81521348104644	23.143680055707602
48-49	20.922652184643038	28.043861244335623	30.180467470797538	20.853019100223804
50-51	21.929547633718986	30.351747182209305	28.301284619656975	19.417420564414737
52-53	20.453289826020836	30.32738769087771	28.293206679940614	20.926115803160844
54-55	22.290565237946605	28.332320826440544	28.201889671237833	21.17522426437502
56-57	21.65724416047577	28.639559602907518	29.35927073049237	20.343925506124343
58-59	21.28302443424252	27.85807298301298	29.056924912328945	21.801977670415553
60-61	19.55752880269787	30.336216697352818	29.594429898194285	20.511824601755023
62-63	20.54835807964649	31.002400130991603	27.264545007983166	21.184696781378744
64-65	19.872736425895177	30.06307914295262	28.949673983156444	21.11451044799576
66-67	20.25524527697107	30.10861861247814	29.453874306777895	20.182261803772896
68-69	21.00578686753413	29.80328311646684	29.183165375783716	20.00776464021531
70-71	19.626008919664987	31.08510501606811	29.104154635158626	20.184731429108282
72-73	20.144129406985968	30.679257495917344	28.452898891120952	20.72371420597573
74-75	20.091887332583582	29.07116873032935	30.46932207230234	20.36762186478473
76-77	19.591606866041996	30.33922635445997	28.734401639140415	21.334765140357614
78-79	19.732030108014158	29.32963315529004	28.490288673296543	22.448048063399252
80-81	20.631037319231226	29.597058134247405	28.461770545731436	21.310134000789937
82-83	20.250634158337867	28.415163706800556	30.003029176809974	21.3311729580516
84-85	20.51162001895567	29.039875296009637	28.37922461363316	22.069280071401533
86-87	21.75499865983968	28.666409226934736	29.04227584091606	20.536316272309524
88-89	21.36775104518689	28.38813598896924	28.653871129078162	21.59024183676571
90-91	21.954002105571472	28.565465450114257	28.100882295238698	21.379650149075566
92-93	22.36983174770722	28.161603992296154	29.344986280626806	20.12357797936982
94-95	21.657577984896257	28.621420597573344	29.138608499103334	20.582392918427068
96-97	21.730285136378583	28.61649861700981	28.9780483120888	20.675167934522808
98-99	22.62881427588513	28.8717162618093	28.3511952295819	20.148274232723676
100	22.902821797362233	30.498111341072182	26.914909490821977	19.68415737074361
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	8.0
2	15.5
3	22.0
4	27.5
5	27.5
6	28.5
7	39.0
8	61.0
9	72.0
10	74.5
11	85.0
12	103.0
13	119.5
14	142.5
15	181.5
16	245.0
17	340.0
18	493.0
19	767.5
20	1221.0
21	1918.5
22	2971.0
23	4653.0
24	7312.0
25	10816.5
26	15277.0
27	20525.0
28	26387.0
29	33405.5
30	41445.5
31	50566.0
32	60556.5
33	71455.5
34	83763.0
35	97785.5
36	111549.5
37	121592.5
38	128871.0
39	134872.0
40	138581.5
41	139551.5
42	138784.0
43	137893.5
44	137172.0
45	133315.0
46	123445.5
47	112681.5
48	106138.5
49	101403.0
50	96586.0
51	89274.0
52	78393.5
53	67218.0
54	56681.5
55	46361.0
56	35743.5
57	26323.5
58	19238.0
59	14005.5
60	10886.0
61	9163.5
62	7708.5
63	6114.0
64	4669.0
65	4095.5
66	4522.0
67	4212.0
68	2883.5
69	2456.0
70	2936.5
71	2722.5
72	1624.5
73	736.5
74	394.5
75	256.0
76	180.5
77	132.0
78	93.5
79	79.0
80	64.0
81	49.5
82	38.5
83	30.0
84	31.0
85	26.5
86	18.5
87	20.0
88	20.5
89	16.5
90	15.5
91	16.0
92	17.5
93	18.0
94	16.0
95	15.0
96	19.5
97	20.5
98	37.5
99	92.0
100	101.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.365423034730876
2	0.005526434316946534
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	6.389939678969431E-4
30-31	0.009377668231568652
32-33	0.0
34-35	0.0
36-37	0.0
38-39	8.80775469263354E-4
40-41	0.0015888498661221287
42-43	3.454021448091584E-5
44-45	0.0
46-47	0.002055142761614492
48-49	1.727010724045792E-5
50-51	0.0
52-53	0.0010534765416679332
54-55	0.01884168699933959
56-57	0.01153643163662589
58-59	0.01404059718649229
60-61	1.5543096516412128E-4
62-63	0.01153643163662589
64-65	1.2089075068320543E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	2.2451139412595295E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2895176.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.13122034446795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.01578686410323	63.764552491256275
2	8.691260752035257	12.885875317607038
3	2.4362554449785927	5.418077676213537
4	1.0078232837036796	2.988446796500908
5	0.5365798170328362	1.988865832442774
6	0.32558996322575157	1.4481828783501245
7	0.2124090919840557	1.1022301640726877
8	0.14278369210432862	0.8467783472786122
9	0.11305243763325987	0.7542643648203368
>10	0.4848905002661669	6.097840991456429
>50	0.023501186449112966	1.1848053501504492
>100	0.00954561551615694	1.2614869696702107
>500	4.739554251274002E-4	0.2217011814526304
>1k	4.739554247636784E-5	0.03689163872794275
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.908042896183168E-5	0.0
3	0.0	0.0	0.0	6.908042896183168E-5	0.0
4	0.0	0.0	0.0	6.908042896183168E-5	0.0
5	0.0	0.0	0.0	6.908042896183168E-5	0.0
6	0.0	0.0	0.0	6.908042896183168E-5	0.0
7	0.0	0.0	0.0	6.908042896183168E-5	0.0
8	0.0	0.0	0.0	6.908042896183168E-5	0.0
9	0.0	0.0	0.0	6.908042896183168E-5	0.0
10-11	0.0	0.0	0.0	6.908042896183168E-5	0.0
12-13	0.0	0.0	0.0	6.908042896183168E-5	0.0
14-15	0.0	0.0	0.0	6.908042896183168E-5	0.0
16-17	0.0	0.0	0.0	6.908042896183168E-5	0.0
18-19	0.0	0.0	0.0	6.908042896183168E-5	0.0
20-21	0.0	0.0	0.0	6.908042896183168E-5	0.0
22-23	0.0	0.0	1.727010724045792E-5	6.908042896183168E-5	0.0
24-25	0.0	0.0	3.454021448091584E-5	6.908042896183168E-5	0.0
26-27	0.0	0.0	3.454021448091584E-5	6.908042896183168E-5	0.0
28-29	0.0	0.0	3.454021448091584E-5	6.908042896183168E-5	0.0
30-31	0.0	0.0	3.454021448091584E-5	6.908042896183168E-5	0.0
32-33	0.0	0.0	3.454021448091584E-5	6.908042896183168E-5	0.0
34-35	0.0	0.0	3.454021448091584E-5	6.908042896183168E-5	0.0
36-37	3.454021448091584E-5	0.0	3.454021448091584E-5	6.908042896183168E-5	0.0
38-39	3.454021448091584E-5	0.0	3.454021448091584E-5	6.908042896183168E-5	0.0
40-41	3.454021448091584E-5	0.0	3.454021448091584E-5	6.908042896183168E-5	0.0
42-43	3.454021448091584E-5	0.0	3.454021448091584E-5	6.908042896183168E-5	3.454021448091584E-5
44-45	3.454021448091584E-5	0.0	3.454021448091584E-5	6.908042896183168E-5	3.454021448091584E-5
46-47	6.908042896183168E-5	0.0	3.454021448091584E-5	6.908042896183168E-5	3.454021448091584E-5
48-49	1.2089075068320545E-4	0.0	3.454021448091584E-5	6.908042896183168E-5	3.454021448091584E-5
50-51	1.3816085792366336E-4	0.0	3.454021448091584E-5	6.908042896183168E-5	3.454021448091584E-5
52-53	1.727010724045792E-4	0.0	3.454021448091584E-5	6.908042896183168E-5	3.454021448091584E-5
54-55	2.0724128688549503E-4	0.0	3.454021448091584E-5	5.871836461755693E-4	3.454021448091584E-5
56-57	2.0724128688549503E-4	0.0	3.454021448091584E-5	5.871836461755693E-4	3.454021448091584E-5
58-59	2.935918230877846E-4	0.0	3.454021448091584E-5	5.871836461755693E-4	3.454021448091584E-5
60-61	4.31752681011448E-4	0.0	3.454021448091584E-5	5.871836461755693E-4	3.454021448091584E-5
62-63	4.4902278825190595E-4	0.0	3.454021448091584E-5	5.871836461755693E-4	3.454021448091584E-5
64-65	5.181032172137377E-4	0.0	3.454021448091584E-5	5.871836461755693E-4	3.454021448091584E-5
66-67	5.871836461755693E-4	0.0	3.454021448091584E-5	5.871836461755693E-4	3.454021448091584E-5
68-69	6.389939678969431E-4	0.0	3.454021448091584E-5	5.871836461755693E-4	3.454021448091584E-5
70-71	7.253445040992326E-4	0.0	3.454021448091584E-5	5.871836461755693E-4	3.454021448091584E-5
72-73	7.944249330610643E-4	0.0	3.454021448091584E-5	5.871836461755693E-4	3.454021448091584E-5
74-75	8.289651475419802E-4	0.0	3.454021448091584E-5	5.871836461755693E-4	3.454021448091584E-5
76-77	9.153156837442698E-4	0.0	3.454021448091584E-5	5.871836461755693E-4	5.1810321721373765E-5
78-79	0.0011225569706297647	0.0	3.454021448091584E-5	5.871836461755693E-4	6.908042896183168E-5
80-81	0.0013816085792366336	0.0	3.454021448091584E-5	5.871836461755693E-4	6.908042896183168E-5
82-83	0.0015715797588816706	0.0	3.454021448091584E-5	5.871836461755693E-4	6.908042896183168E-5
84-85	0.0016233900806030444	0.0	3.454021448091584E-5	5.871836461755693E-4	6.908042896183168E-5
86-87	0.0016924705095648761	0.0	3.454021448091584E-5	5.871836461755693E-4	6.908042896183168E-5
88	0.0016924705095648761	0.0	3.454021448091584E-5	5.871836461755693E-4	6.908042896183168E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAGCC	30	7.1564864E-7	88.2744	1
TCACCGG	10985	0.0	79.8617	8
CACCGGC	10625	0.0	77.6639	9
CTTCACC	12390	0.0	74.06359	6
GTGTGGC	4660	0.0	70.609215	7
CCTTCAC	13790	0.0	70.5269	5
ATGCAGC	80	0.0	70.41263	2
CGTAGTG	2260	0.0	65.83853	4
CGGGGCA	1365	0.0	65.335655	94
TTCACCG	13935	0.0	64.90887	7
CGGGCCC	745	0.0	63.634754	7
ACGCAGC	30	1.3799511E-4	62.589	2
TGTGGCA	5495	0.0	61.588104	8
GCCTTCA	4055	0.0	61.581474	4
GGGCCCG	775	0.0	61.171474	8
GCGTAGT	1455	0.0	60.649067	3
ACCTTCA	5435	0.0	60.36802	4
TGTGTGG	5340	0.0	58.98079	6
GCAGCCA	80	1.8189894E-12	58.677193	2
GGCCCGA	820	0.0	58.38693	9
>>END_MODULE