FastQCFastQC Report
Tue 7 Mar 2017
1NF_23A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_23A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3206742
Sequences flagged as poor quality0
Sequence length95
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA169920.5298836014871168No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT71270.22225049598626895No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA69800.21766640409487262No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG68000.21205323035030568No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA67370.21008861953970728No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG59510.18557776085509842No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG58680.18298946407288144No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG54580.17020390165470126No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG48150.15015239766716498No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC46610.1453500156857022No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT44180.13777223113053685No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT43270.13493445995967246No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA42520.1325956375661029No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA42180.1315353714143514No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA41940.1307869482484091No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC41460.12929010191652462No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC41400.12910299612503906No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG39680.12373929676911956No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA39520.12324034799182472No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA34890.1088020177488554No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA32900.10259634233125085No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA32850.1024404208383462No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA32570.10156726047808025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC950.088.999893
GCTGCCA950.088.999893
AGCTGCC1900.088.999892
GCAGCTA550.088.999893
GCAGCCG256.816608E-788.999893
GCTGCAC401.6370905E-1188.9998863
TGCAGCG202.4113919E-588.9998862
CAGCTGC5650.088.212291
AGCTGCT1450.085.930932
CAGCAGC5000.085.4398961
AGCTGCG1200.085.291562
AGCTGCA1200.085.291562
AGCAGCG1100.084.9544452
AGCAGCA2000.084.54992
TGCTGCC850.083.76462
CAGCCCT177550.083.235331
CAGCGTA61500.081.691771
TGCTGCA550.080.9092
GCAGCAC550.080.9093
AGCCCTT150200.080.852432
Produced by FastQC (version 0.11.5)