##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1NF_23A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3206742
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.820277403046456	12.0	12.0	12.0	2.0	32.0
2	14.568285817817586	12.0	12.0	12.0	12.0	22.0
3	13.713168692710546	12.0	12.0	12.0	12.0	22.0
4	13.78598402989701	12.0	12.0	12.0	12.0	22.0
5	22.434058929592716	22.0	22.0	22.0	22.0	27.0
6	26.83763395995063	27.0	22.0	32.0	22.0	32.0
7	34.95632794905234	37.0	37.0	37.0	27.0	41.0
8	37.581446215504705	41.0	37.0	41.0	27.0	41.0
9	38.526732428115515	41.0	41.0	41.0	32.0	41.0
10-11	38.88176847404625	41.0	41.0	41.0	37.0	41.0
12-13	39.063104546608365	41.0	41.0	41.0	37.0	41.0
14-15	39.014554335833694	41.0	41.0	41.0	37.0	41.0
16-17	38.90450744088548	41.0	41.0	41.0	37.0	41.0
18-19	38.85103478857981	41.0	41.0	41.0	37.0	41.0
20-21	38.86106833664822	41.0	41.0	41.0	37.0	41.0
22-23	38.84839082782463	41.0	41.0	41.0	34.5	41.0
24-25	38.866207820897344	41.0	41.0	41.0	37.0	41.0
26-27	38.822765598230234	41.0	41.0	41.0	37.0	41.0
28-29	38.766356632370176	41.0	41.0	41.0	34.5	41.0
30-31	38.64366746685577	41.0	41.0	41.0	32.0	41.0
32-33	38.71638862746052	41.0	41.0	41.0	32.0	41.0
34-35	38.688394014859945	41.0	41.0	41.0	32.0	41.0
36-37	38.611254818753736	41.0	41.0	41.0	32.0	41.0
38-39	38.5135322704477	41.0	41.0	41.0	32.0	41.0
40-41	38.55808371861534	41.0	41.0	41.0	32.0	41.0
42-43	38.29753126381854	41.0	41.0	41.0	32.0	41.0
44-45	38.40649996164331	41.0	41.0	41.0	32.0	41.0
46-47	38.41356008684203	41.0	41.0	41.0	32.0	41.0
48-49	38.37352194220801	41.0	41.0	41.0	32.0	41.0
50-51	38.259213868780215	41.0	41.0	41.0	32.0	41.0
52-53	38.21370958436943	41.0	41.0	41.0	32.0	41.0
54-55	38.09590996095102	41.0	37.0	41.0	32.0	41.0
56-57	37.88385688652221	41.0	37.0	41.0	29.5	41.0
58-59	37.861758757018805	41.0	37.0	41.0	29.5	41.0
60-61	37.87981041193835	41.0	37.0	41.0	29.5	41.0
62-63	37.868674654836596	41.0	37.0	41.0	29.5	41.0
64-65	37.84868879379757	41.0	37.0	41.0	29.5	41.0
66-67	37.722978805279624	41.0	37.0	41.0	27.0	41.0
68-69	37.73693315520862	41.0	37.0	41.0	27.0	41.0
70-71	37.71247109995129	41.0	37.0	41.0	27.0	41.0
72-73	37.58056136103248	41.0	37.0	41.0	27.0	41.0
74-75	37.476504657998674	41.0	37.0	41.0	27.0	41.0
76-77	36.39410264374247	39.0	34.5	41.0	24.5	41.0
78-79	36.93187992672937	41.0	37.0	41.0	27.0	41.0
80-81	37.60854583873601	41.0	37.0	41.0	27.0	41.0
82-83	37.79010955667778	41.0	37.0	41.0	27.0	41.0
84-85	37.72044414549097	41.0	37.0	41.0	27.0	41.0
86-87	37.59287494909164	41.0	37.0	41.0	27.0	41.0
88-89	37.505664471915736	41.0	37.0	41.0	27.0	41.0
90-91	37.345130509407994	41.0	37.0	41.0	27.0	41.0
92-93	37.21997466587584	41.0	37.0	41.0	27.0	41.0
94-95	37.068126621973335	41.0	37.0	41.0	27.0	41.0
96-97	37.00585734680246	41.0	37.0	41.0	27.0	41.0
98-99	36.88447480339859	41.0	37.0	41.0	27.0	41.0
100	36.74217539172157	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.2549685785688585
1101	2	-0.4728911121978321
1101	3	0.07720736634576753
1101	4	-0.11655659910051419
1101	5	-1.1657306058131596
1101	6	-0.8907980334946153
1101	7	-0.4281940110617697
1101	8	-0.24753703651950332
1101	9	-0.2789050360249874
1101	10-11	-0.19262178699393218
1101	12-13	-0.11783148586298609
1101	14-15	-0.09613334974746834
1101	16-17	-0.18903499982729954
1101	18-19	-0.061109193561513564
1101	20-21	-0.1434186266243742
1101	22-23	-0.1194163537743691
1101	24-25	-0.028017674175295326
1101	26-27	-0.02264026490031057
1101	28-29	-0.10864847423800228
1101	30-31	-0.11423446403559723
1101	32-33	-0.3265558297590374
1101	34-35	0.006227524935361828
1101	36-37	0.11569214445074749
1101	38-39	-0.05402752590543969
1101	40-41	-0.1852233828371581
1101	42-43	0.08098685217324686
1101	44-45	-0.16659997647079905
1101	46-47	-0.10463377030762899
1101	48-49	-0.12459146168288271
1101	50-51	-0.030395511041525936
1101	52-53	-0.03493539247581623
1101	54-55	-0.052357793005157305
1101	56-57	0.10603820904762529
1101	58-59	-0.19547115122188785
1101	60-61	-0.13171319812580862
1101	62-63	-0.2952890247563573
1101	64-65	-0.1639930889021457
1101	66-67	-0.21833827280652685
1101	68-69	-0.1741488989120228
1101	70-71	-0.20290310002948786
1101	72-73	-0.024195109741611986
1101	74-75	-0.11402150168831326
1101	76-77	-0.4083005296869331
1101	78-79	-0.27525723527616464
1101	80-81	-0.24369478035637115
1101	82-83	-0.25257815971021813
1101	84-85	-0.10198825233399589
1101	86-87	-0.17325713743491633
1101	88-89	-0.38951816135379147
1101	90-91	-0.363217601101951
1101	92-93	-0.18479730739765898
1101	94-95	-0.09732739953687997
1101	96-97	-0.2711735765087937
1101	98-99	-0.47226288038761766
1101	100	-0.9298423155083526
1102	1	1.7453563942963122
1102	2	0.7454552965850496
1102	3	-0.062395798030767224
1102	4	0.08924615920657075
1102	5	-0.8083688433065745
1102	6	-0.07589881205144167
1102	7	-0.07178274559473152
1102	8	-0.38463833646910217
1102	9	-0.3030573871929718
1102	10-11	-0.3138622117499352
1102	12-13	-0.4043405473012598
1102	14-15	-0.37513831044152113
1102	16-17	-0.3265898443273798
1102	18-19	-0.24597035780968923
1102	20-21	-0.11653169970244903
1102	22-23	-0.199828191877927
1102	24-25	-0.10400090751696922
1102	26-27	-0.22708649403153913
1102	28-29	-0.33698523874706865
1102	30-31	-0.31500071987328226
1102	32-33	-0.2763325338502298
1102	34-35	-0.348290955403705
1102	36-37	-0.3521830580989018
1102	38-39	-0.2755241818772589
1102	40-41	-0.333819204431272
1102	42-43	-0.251275669089182
1102	44-45	-0.2005049969160666
1102	46-47	-0.548962280591951
1102	48-49	-0.4639756833822801
1102	50-51	-0.38697263463499354
1102	52-53	-0.28701045363249733
1102	54-55	-0.42552010735226986
1102	56-57	-0.2479183312725084
1102	58-59	-0.3503677159499645
1102	60-61	-0.1906739274985796
1102	62-63	-0.5138942020672133
1102	64-65	-0.365639941316644
1102	66-67	-0.3486583572243944
1102	68-69	-0.5024208127071006
1102	70-71	-0.4482621197513694
1102	72-73	-0.39223615797990163
1102	74-75	-0.4248507002807571
1102	76-77	-0.3075570474697642
1102	78-79	-0.47689898819071885
1102	80-81	-0.3962647293265462
1102	82-83	-0.39152006819682583
1102	84-85	-0.39226646612593186
1102	86-87	-0.39687126747763557
1102	88-89	-0.4739699850096031
1102	90-91	-0.47945268008947295
1102	92-93	-0.4000614415946089
1102	94-95	-0.27934086619683995
1102	96-97	-0.26826101576877903
1102	98-99	-0.42988516515265474
1102	100	-0.7905222355680976
1103	1	1.5869026936393507
1103	2	0.9728172369211379
1103	3	0.477244431617045
1103	4	0.8818314717264517
1103	5	2.88990344358567
1103	6	-0.08814817419397514
1103	7	-0.5987178412628396
1103	8	-0.2834256264140649
1103	9	-0.43335968694754
1103	10-11	-0.37697915311422037
1103	12-13	-0.519387048140068
1103	14-15	-0.32354506596180954
1103	16-17	-0.4158872124394435
1103	18-19	-0.27253458906538697
1103	20-21	-0.3086675765698601
1103	22-23	-0.21517208543410504
1103	24-25	-0.3029022232888252
1103	26-27	-0.26602305020934125
1103	28-29	-0.11954280591732669
1103	30-31	-0.17266833841252804
1103	32-33	-0.3460231074814786
1103	34-35	-0.4427315971457517
1103	36-37	-0.4241489849788138
1103	38-39	-0.20955888963571567
1103	40-41	-0.3499184043509018
1103	42-43	-0.3897044125727618
1103	44-45	-0.6832353239231139
1103	46-47	-0.7834161582031243
1103	48-49	-0.5637726959923057
1103	50-51	-0.39594668649252895
1103	52-53	-0.4174336763486295
1103	54-55	-0.454009799323174
1103	56-57	-0.5483391584773614
1103	58-59	-0.5771651366510895
1103	60-61	-0.4435375451387671
1103	62-63	-0.38533078342401694
1103	64-65	-0.6899817780988187
1103	66-67	-0.5251523358728534
1103	68-69	-0.5106568966542753
1103	70-71	-0.6279642859233618
1103	72-73	-0.4504846274470822
1103	74-75	-0.5482867813721839
1103	76-77	-0.8363190551453386
1103	78-79	-0.611636171026106
1103	80-81	-0.42177405594819817
1103	82-83	-0.44599806155645894
1103	84-85	-0.48886740263188244
1103	86-87	-0.4133148141755072
1103	88-89	-0.41494619346471495
1103	90-91	-0.5812316946202429
1103	92-93	-0.3738943374082311
1103	94-95	-0.5101451906620227
1103	96-97	-0.4125979646370368
1103	98-99	-0.43177983736472925
1103	100	-0.24452159837994003
1104	1	1.6848674308693532
1104	2	-0.4361271922708152
1104	3	-0.0038040112167934126
1104	4	0.012363216054488646
1104	5	-1.2000211425745952
1104	6	-0.6054343715949067
1104	7	-0.24603319392556955
1104	8	-0.24030195574303548
1104	9	-0.009363320367100414
1104	10-11	-0.10968496142586304
1104	12-13	-0.007609328824280226
1104	14-15	-0.15115593335155353
1104	16-17	-0.27690056958789455
1104	18-19	-0.10683912746376478
1104	20-21	-0.10157979674590933
1104	22-23	-0.11661341405282855
1104	24-25	-0.115430577529402
1104	26-27	-0.2155422145228414
1104	28-29	-0.08806837266771339
1104	30-31	-0.1725176253921603
1104	32-33	-0.1485161785682081
1104	34-35	-0.14852895246985298
1104	36-37	-0.3275738523723817
1104	38-39	-0.23174644517371235
1104	40-41	-0.23660673734132587
1104	42-43	-0.26694706499451826
1104	44-45	-0.12506895229130066
1104	46-47	-0.33604188350157926
1104	48-49	-0.3496829395728227
1104	50-51	-0.1756264181392666
1104	52-53	-0.1662192772037301
1104	54-55	-0.26235914777281977
1104	56-57	-0.22850364332992967
1104	58-59	-0.1846405916407008
1104	60-61	-0.18588147021286971
1104	62-63	-0.3336465367566106
1104	64-65	-0.34524192367766204
1104	66-67	-0.4826616471474736
1104	68-69	-0.42595229353720754
1104	70-71	-0.12120296604420844
1104	72-73	-0.22312403539843473
1104	74-75	-0.47039375704088826
1104	76-77	-0.4473272844839258
1104	78-79	-0.4390779162395688
1104	80-81	-0.3197781594700828
1104	82-83	-0.17387168938957132
1104	84-85	-0.26718886980975753
1104	86-87	-0.3764438865642603
1104	88-89	-0.2694160193293982
1104	90-91	-0.5093982127609067
1104	92-93	-0.4092479428955613
1104	94-95	-0.44931082911256937
1104	96-97	-0.29969690461363996
1104	98-99	-0.38520588319907034
1104	100	-0.2871363100345974
1105	1	1.8480258700978993
1105	2	0.35941600944539687
1105	3	-0.053612188745347567
1105	4	-0.158834235646081
1105	5	-1.1087733641904123
1105	6	0.3078114455105876
1105	7	0.15177297400473577
1105	8	0.11063448358834904
1105	9	0.10382352209191481
1105	10-11	-0.10559743611229777
1105	12-13	-0.11815829481660245
1105	14-15	-0.089692271831332
1105	16-17	-0.08217601278050779
1105	18-19	-0.13616600497304887
1105	20-21	0.04275117243922466
1105	22-23	-0.05521702823227059
1105	24-25	0.009165195252187175
1105	26-27	0.01594120226498319
1105	28-29	-0.01146724508733854
1105	30-31	0.015421130180854448
1105	32-33	-0.10235036688937527
1105	34-35	-0.05977093250643861
1105	36-37	-0.13559496182672603
1105	38-39	-0.1074392269123905
1105	40-41	-0.06353665477062265
1105	42-43	0.09900820967431656
1105	44-45	0.11643309230765908
1105	46-47	-0.14158673923360965
1105	48-49	0.012940577310835977
1105	50-51	-0.0936486860241601
1105	52-53	-0.056468575176232605
1105	54-55	-0.13457149614696107
1105	56-57	0.0510931732781188
1105	58-59	0.03279019523099436
1105	60-61	-0.06233540896968037
1105	62-63	-0.23416696323251784
1105	64-65	-0.157884241827297
1105	66-67	-0.14784126892919147
1105	68-69	-0.12003054521150602
1105	70-71	-0.443133419325072
1105	72-73	-0.34848288559106066
1105	74-75	-0.25805126797628475
1105	76-77	-0.3572789463361872
1105	78-79	-0.21387567099052518
1105	80-81	-0.34226856215168766
1105	82-83	-0.2713681165295583
1105	84-85	-0.10923413780678004
1105	86-87	-0.09262301090226543
1105	88-89	-0.15968309642325806
1105	90-91	-0.18862253509452387
1105	92-93	-0.02258284145234768
1105	94-95	0.01610983295217494
1105	96-97	-0.14566485387562267
1105	98-99	-0.3431166320098171
1105	100	-0.38530506053405134
1106	1	1.2256168716606606
1106	2	-0.6233016512835299
1106	3	-0.07972275937396311
1106	4	-0.12518832953151815
1106	5	-1.0685744578898344
1106	6	-0.4792277282753439
1106	7	-0.11918780524411687
1106	8	0.03693265689958736
1106	9	-0.09039068282076812
1106	10-11	0.11589409312066579
1106	12-13	0.15282854889827746
1106	14-15	0.11762707283423168
1106	16-17	0.1925562455247487
1106	18-19	0.0846680112206073
1106	20-21	0.14103996647695283
1106	22-23	0.1407551757359542
1106	24-25	-0.09047071366429549
1106	26-27	-0.002767797684484208
1106	28-29	0.05803457567544257
1106	30-31	0.15042406058909563
1106	32-33	-0.01381060523907962
1106	34-35	0.053479110924094186
1106	36-37	-0.13378114547128916
1106	38-39	-0.023493511818401203
1106	40-41	0.05572862481565011
1106	42-43	0.18575905145207372
1106	44-45	0.16761030252212095
1106	46-47	-0.13821852172711857
1106	48-49	-0.12129892242845841
1106	50-51	-0.19675295163386153
1106	52-53	-0.16307924914061545
1106	54-55	-0.17357899816419575
1106	56-57	-0.08648915570240945
1106	58-59	-0.17058954227769618
1106	60-61	-0.10709719511835658
1106	62-63	-0.24055785965220622
1106	64-65	-0.3391703486431652
1106	66-67	-0.14753255254144193
1106	68-69	-0.1999669429965678
1106	70-71	-0.39200139161658143
1106	72-73	-0.25987206138715635
1106	74-75	-0.37247538036135097
1106	76-77	-0.24869966751291628
1106	78-79	-0.2195877233102692
1106	80-81	-0.0686569529401666
1106	82-83	-0.24798478655058886
1106	84-85	-0.3922144472091986
1106	86-87	-0.17978344592803808
1106	88-89	-0.2633064383328616
1106	90-91	-0.43684385044271323
1106	92-93	-0.3080234766187999
1106	94-95	-0.20653812254250425
1106	96-97	-0.1202740067571142
1106	98-99	-0.18107083452998296
1106	100	-0.41779970410186706
1107	1	1.0996643766291
1107	2	-0.5704970966361298
1107	3	-0.08960594828869795
1107	4	-0.21092306486447043
1107	5	-1.2458664628929554
1107	6	-0.34589397529989796
1107	7	-0.3839513702371775
1107	8	-0.13690816198509737
1107	9	-0.16620557226605115
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2224	10-11	0.1318883453550015
2224	12-13	0.0622608046575337
2224	14-15	-0.05131457109826698
2224	16-17	-9.236687915006314E-4
2224	18-19	0.050385751819803204
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2224	22-23	0.0672421119395139
2224	24-25	0.04854220980956825
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2224	28-29	0.05444630162613606
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2224	50-51	0.1699449951453147
2224	52-53	0.2607462378278598
2224	54-55	0.19218672447180296
2224	56-57	0.3597573447825084
2224	58-59	0.3628408829890475
2224	60-61	0.29979917580065774
2224	62-63	0.38350774147489375
2224	64-65	0.1735879657535193
2224	66-67	0.15679072616148204
2224	68-69	0.20886022796262438
2224	70-71	0.29218003831530837
2224	72-73	0.2608314855760341
2224	74-75	0.2296935420849735
2224	76-77	0.3562949037617642
2224	78-79	0.21392673951229568
2224	80-81	0.2190903858205715
2224	82-83	0.21777074935140917
2224	84-85	0.030184297074853816
2224	86-87	0.29652699820996276
2224	88-89	0.21233652875918096
2224	90-91	0.32765385778211
2224	92-93	0.3022642968167162
2224	94-95	0.49101846512478176
2224	96-97	0.2756250109129681
2224	98-99	0.2703562621104467
2224	100	0.38775594144250647
2225	1	1.3552623367416157
2225	2	1.6636511036474406
2225	3	0.8564868336591047
2225	4	1.1299170408117654
2225	5	3.2896397032542204
2225	6	0.1779557950208961
2225	7	0.40561613728319657
2225	8	0.44450154423827115
2225	9	0.25746646807316154
2225	10-11	0.2410080146076652
2225	12-13	0.27972302103392366
2225	14-15	0.19535246860591116
2225	16-17	0.36669859956460016
2225	18-19	0.32812244825155545
2225	20-21	0.30440337883335644
2225	22-23	0.30033808244972704
2225	24-25	0.3330724977402255
2225	26-27	0.31905055802059223
2225	28-29	0.2553212752715126
2225	30-31	0.29770907245943334
2225	32-33	0.2696119900330416
2225	34-35	0.334606399198897
2225	36-37	0.3726687797062027
2225	38-39	0.23131931939778383
2225	40-41	0.14394886177234412
2225	42-43	0.37792069057130107
2225	44-45	0.3908992299161582
2225	46-47	0.3244686751854715
2225	48-49	0.347891644309847
2225	50-51	0.26057009032300016
2225	52-53	0.2565231226766116
2225	54-55	0.2938000762870203
2225	56-57	0.4854670309375564
2225	58-59	0.5263567992954847
2225	60-61	0.4349356142698966
2225	62-63	0.4887871902482246
2225	64-65	0.41649759694455923
2225	66-67	0.4314558246540088
2225	68-69	0.47977866047789774
2225	70-71	0.454845936713582
2225	72-73	0.5410691269967884
2225	74-75	0.42398770907686867
2225	76-77	0.4082306969233258
2225	78-79	0.4347632336302283
2225	80-81	0.44688981393991156
2225	82-83	0.4535184145025539
2225	84-85	0.3964344720837545
2225	86-87	0.3671522067155024
2225	88-89	0.36111551560347266
2225	90-91	0.546740203924557
2225	92-93	0.6539661269906816
2225	94-95	0.6017362341527033
2225	96-97	0.5449008978863858
2225	98-99	0.5862424186176298
2225	100	0.7581119490817514
2226	1	1.4040435019397233
2226	2	1.2442954305521123
2226	3	0.7279777353987509
2226	4	0.9481640060915488
2226	5	3.1456032839892565
2226	6	-0.29281996669572763
2226	7	-0.026060431951854923
2226	8	0.2549816902700641
2226	9	0.15476587163045963
2226	10-11	0.09944691957900176
2226	12-13	0.19937800018262664
2226	14-15	0.12977225947071958
2226	16-17	0.15433678276851026
2226	18-19	0.1261763271554983
2226	20-21	0.12250510141061
2226	22-23	0.22998381660539735
2226	24-25	0.24462243345676882
2226	26-27	0.1399446117831573
2226	28-29	0.214432861955963
2226	30-31	0.21002855014355504
2226	32-33	0.19604522022836335
2226	34-35	0.18707277166912917
2226	36-37	0.19670107710604157
2226	38-39	0.08566299998732063
2226	40-41	0.17877212577935353
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2226	44-45	0.06767139948335199
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2226	48-49	0.1712905238014315
2226	50-51	0.1654899380452335
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2226	54-55	0.3441821295575309
2226	56-57	0.3973495289356279
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2226	60-61	0.2665757208519324
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2226	64-65	0.30423935124753854
2226	66-67	0.38117544723515806
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2226	70-71	0.4217272033968271
2226	72-73	0.4251420279864462
2226	74-75	0.42049145439565905
2226	76-77	0.4539456140461837
2226	78-79	0.48674015547666016
2226	80-81	0.37965650803004536
2226	82-83	0.12628940687078227
2226	84-85	0.20142012899438555
2226	86-87	0.2938498775637157
2226	88-89	0.2687214295382603
2226	90-91	0.3856009051470295
2226	92-93	0.25747911706411486
2226	94-95	0.42987698690683374
2226	96-97	0.45079703642110047
2226	98-99	0.4688734416006568
2226	100	0.5764083815109302
2227	1	1.0737939518287671
2227	2	1.7773462437145593
2227	3	0.5899471680073347
2227	4	-0.06959510328280771
2227	5	3.1371611783117004
2227	6	-0.02713828163920695
2227	7	0.30933042776015185
2227	8	0.2948276955035567
2227	9	0.3553165585586697
2227	10-11	0.3348937624943389
2227	12-13	0.2722872891763828
2227	14-15	0.33491805604177927
2227	16-17	0.2593425785399788
2227	18-19	0.17313265157638824
2227	20-21	0.1916210057141825
2227	22-23	0.2787898852384316
2227	24-25	0.3257834681202638
2227	26-27	0.38982886140589557
2227	28-29	0.3405337742779935
2227	30-31	0.396416244646673
2227	32-33	0.33414352008241366
2227	34-35	0.2923908825593955
2227	36-37	0.24144130293878163
2227	38-39	0.22164313212431352
2227	40-41	0.29375226023929457
2227	42-43	0.5537525327237987
2227	44-45	0.44200977008237885
2227	46-47	0.41046801517639153
2227	48-49	0.291862804425584
2227	50-51	0.5657623227988822
2227	52-53	0.5138868019324931
2227	54-55	0.4228808324564213
2227	56-57	0.56667695658507
2227	58-59	0.5845165963837644
2227	60-61	0.535255484336048
2227	62-63	0.4115529616749001
2227	64-65	0.4395229026879619
2227	66-67	0.5280538426457824
2227	68-69	0.49104876242900275
2227	70-71	0.5036972986587429
2227	72-73	0.5005396291569184
2227	74-75	0.4943925226979857
2227	76-77	0.6226246928700121
2227	78-79	0.6395737503868943
2227	80-81	0.5164053547137044
2227	82-83	0.40302071402540207
2227	84-85	0.3897308019066159
2227	86-87	0.4550766738463494
2227	88-89	0.4836308483729539
2227	90-91	0.4797852785343366
2227	92-93	0.31663535397075293
2227	94-95	0.5263840702675253
2227	96-97	0.503099457220884
2227	98-99	0.6112578981497307
2227	100	0.48433416776072846
2228	1	1.3185838116531485
2228	2	1.3389752664365009
2228	3	-0.09353992024106361
2228	4	0.015226569653318478
2228	5	3.282335688237165
2228	6	0.25760501852163387
2228	7	0.46684121851680516
2228	8	0.47162500400410323
2228	9	0.4750362290740142
2228	10-11	0.3193165390293444
2228	12-13	0.3848839909222974
2228	14-15	0.2603046002120095
2228	16-17	0.3467055666524743
2228	18-19	0.3177070406439313
2228	20-21	0.14362474962732819
2228	22-23	0.28973977994993305
2228	24-25	0.26379538788713575
2228	26-27	0.30827966871435564
2228	28-29	0.3370267557095943
2228	30-31	0.24447546720469404
2228	32-33	0.20506587919720687
2228	34-35	0.30028489153711035
2228	36-37	0.23808870991280173
2228	38-39	0.1618833472180654
2228	40-41	0.30948028423398455
2228	42-43	0.6042069099448
2228	44-45	0.5260807028815293
2228	46-47	0.413979202509438
2228	48-49	0.37680700279207713
2228	50-51	0.3601264067527197
2228	52-53	0.3667078412163889
2228	54-55	0.3739143417116182
2228	56-57	0.5310784252952345
2228	58-59	0.5294051653048584
2228	60-61	0.4453401247583457
2228	62-63	0.5160272786409408
2228	64-65	0.4407907508912814
2228	66-67	0.5319191937228211
2228	68-69	0.49152110158809137
2228	70-71	0.5000438295759366
2228	72-73	0.6552542663420837
2228	74-75	0.5619498912432448
2228	76-77	0.4826518949655636
2228	78-79	0.4913582172798314
2228	80-81	0.3712087322105688
2228	82-83	0.39918961931867614
2228	84-85	0.4979160444072832
2228	86-87	0.5216170450694051
2228	88-89	0.5404347978968218
2228	90-91	0.5223233529493143
2228	92-93	0.6077497952602329
2228	94-95	0.8858637141897674
2228	96-97	0.6496144968549586
2228	98-99	0.7534102349598157
2228	100	0.80977077335681
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	29.0
13	53.0
14	179.0
15	579.0
16	2137.0
17	5546.0
18	8713.0
19	11265.0
20	13131.0
21	15064.0
22	17630.0
23	19948.0
24	22681.0
25	25823.0
26	29123.0
27	33023.0
28	37096.0
29	41963.0
30	47897.0
31	55289.0
32	64692.0
33	77515.0
34	94828.0
35	121777.0
36	171897.0
37	282720.0
38	695966.0
39	1308749.0
40	1429.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.071388681563807	18.559213274341577	19.919535582438023	34.449862461656586
2	18.624847538108845	48.91195772139369	14.822086991613077	17.641107748884387
3	39.513562363295826	15.801801329823228	24.951711113647434	19.732925193233505
4	19.731085319617232	15.471310133462563	22.51927345573794	42.27833109118227
5	17.578838584457372	35.551223017006045	26.27214786845964	20.597790530076946
6	17.746298267836952	28.290052645332864	30.672283582527065	23.291365504303123
7	20.52775059546418	26.81709348616134	35.61858110194085	17.036574816433625
8	20.611948201632686	27.88425136789926	33.90207257085229	17.601727859615774
9	20.942532950889095	28.215023222947156	27.63440276766887	23.20804105849488
10-11	19.604664173170153	30.082136324032305	28.946825157745774	21.36637434505177
12-13	23.963901679648693	26.849493972386927	27.277404917514414	21.90919943044997
14-15	22.0039841059867	27.460082538601483	28.28757349359568	22.248359861816137
16-17	20.822722875741174	29.570807380200844	28.295073317404395	21.311396426653594
18-19	21.380048659979504	29.439661812518747	29.773567689574026	19.406721837927716
20-21	22.769808110537113	28.745904721988857	29.87111217553517	18.61317499193886
22-23	21.81335448876149	29.93444748595303	28.737251079132655	19.514946946152826
24-25	22.176137026302708	28.40058539165296	26.546756801763284	22.876520780281044
26-27	20.296238362799375	26.545384692625724	29.88664195622847	23.271734988346427
28-29	19.711781340005963	27.91945195295397	30.656755849880813	21.71201085715925
30-31	20.314740868736443	29.919017045669843	28.242971006096994	21.523271079496713
32-33	20.86234252708824	29.918933297409023	28.641562058937076	20.57716211656566
34-35	21.3619149903547	29.096338277292027	29.884287541685612	19.657459190667662
36-37	22.04032940598277	28.01912345926177	28.71729312804086	21.223254006714605
38-39	21.619476629510263	29.10272662210393	29.042446865405875	20.235349882979932
40-41	20.94783199036267	29.524554393508335	29.78093970212964	19.74667391399936
42-43	20.59163851371105	29.70766091466454	29.256049409703973	20.444651161920437
44-45	20.708136170605556	30.008588155829187	28.668926280941843	20.614349392623417
46-47	20.22939946867115	28.114789858785276	28.782958183814582	22.872852488728988
48-49	21.13043394198847	28.107811604425926	30.0643144973933	20.697439956192298
50-51	22.02729436917594	30.39703537110251	28.242200339160433	19.333469920561118
52-53	20.519535234585913	30.04161920084024	28.361632996984742	21.0772125675891
54-55	22.157730847644665	28.40116234188216	28.15868808137953	21.282418729093653
56-57	21.531784155546532	28.72690595462873	29.293797363104296	20.44751252672045
58-59	21.250631957576115	28.06387535566619	29.056216388511768	21.629276298245927
60-61	19.824821820325035	30.043595744581324	29.56472861025311	20.566853824840532
62-63	20.563522385266737	31.070563101345765	27.268592012724753	21.097322500662745
64-65	20.00156233457691	30.144154210218822	28.787682940881226	21.066600514323035
66-67	20.214816159204577	30.011254413357857	29.516281634132085	20.25764779330548
68-69	20.92008025591083	29.84689756768708	29.143192685909874	20.08982949049222
70-71	19.613395776772812	30.74609681726812	29.299987339174777	20.340520066784293
72-73	20.227274286487656	30.487610166330814	28.378101512376112	20.907014034805417
74-75	20.209015879668524	29.127491391574377	30.433318302501416	20.230174426255683
76-77	19.76641712990942	30.221670468032663	28.72301544683046	21.288896955227454
78-79	19.83854641252711	29.334227699016637	28.419015312114286	22.40821057634197
80-81	20.728099056547876	29.464511244150593	28.41359776021335	21.39379193908818
82-83	20.47386412751634	28.444633213398525	29.792948731142076	21.288553927943067
84-85	20.624952054140934	29.052711443577312	28.20125535512367	22.12108114715808
86-87	21.713736246944716	28.378834343392768	29.222307251409685	20.68512215825283
88-89	21.067675541094356	28.82866785042264	29.310730330035906	20.792926278447098
90-91	21.316760126009513	29.440784447267664	28.564334143501412	20.67812128322141
92-93	21.422646411841054	29.326930573148697	29.004937098151334	20.245485916858918
94-95	21.099951290125617	29.49312729243575	28.65283518287408	20.75408623456455
96-97	21.034620184598573	29.335740137497808	28.90531885633456	20.724320821569055
98-99	22.1022458308152	29.62162219473846	28.28375341701952	19.99237855742682
100	22.2016613746912	31.122834328424304	26.909554931453794	19.765949365430707
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	14.0
2	27.5
3	30.0
4	30.0
5	36.5
6	43.5
7	53.0
8	68.0
9	80.0
10	85.0
11	93.0
12	113.5
13	146.5
14	188.5
15	256.0
16	318.0
17	409.0
18	568.5
19	888.0
20	1408.5
21	2159.0
22	3411.0
23	5444.5
24	8189.0
25	12084.5
26	17477.0
27	23505.5
28	30100.0
29	37928.0
30	47275.5
31	58015.5
32	69332.5
33	81677.5
34	95379.5
35	110482.5
36	125409.5
37	136590.0
38	144243.5
39	149698.5
40	152872.0
41	154029.5
42	152520.5
43	149533.0
44	147308.5
45	143509.0
46	135103.5
47	124087.5
48	114475.0
49	108345.0
50	104553.5
51	98183.5
52	87746.0
53	75488.5
54	62792.0
55	50307.5
56	38671.0
57	28677.0
58	20627.5
59	15253.0
60	12445.5
61	10903.5
62	9175.5
63	7162.5
64	5882.5
65	5750.0
66	5368.5
67	4026.5
68	3031.5
69	3075.0
70	3677.5
71	3486.0
72	2211.0
73	1055.0
74	511.5
75	320.5
76	215.5
77	141.5
78	118.0
79	96.5
80	71.0
81	44.5
82	32.5
83	25.5
84	28.0
85	35.0
86	26.5
87	14.0
88	14.0
89	16.5
90	16.0
91	15.5
92	14.5
93	13.0
94	12.0
95	16.0
96	20.5
97	22.5
98	45.0
99	105.5
100	114.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.381925954754077
2	0.006330412611928243
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	3.118429858092731E-4
30-31	0.010181673486672767
32-33	0.0
34-35	0.0
36-37	0.0
38-39	8.419760616850374E-4
40-41	0.001403293436141729
42-43	1.0914504503324559E-4
44-45	0.0
46-47	0.002026979407760275
48-49	0.0
50-51	0.0
52-53	0.0015280306304654382
54-55	0.019599331658112815
56-57	0.012348982238047214
58-59	0.01356516988270338
60-61	2.1829009006649118E-4
62-63	0.012224245043723506
64-65	7.796074645231827E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	2.4947438864741846E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3206742.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.30602369870225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.15104155494096	64.01541335449325
2	8.589775221206805	12.765440823070366
3	2.4407885075213906	5.440958660502136
4	1.0153782859078464	3.0179489190326443
5	0.5401159443757052	2.0066934081414045
6	0.3065702452975924	1.3668009547439919
7	0.20517406813312272	1.0671968418341278
8	0.13711390299102913	0.8150711140058378
9	0.10776562150762732	0.7206871343086019
>10	0.47186603495183593	6.04065367334782
>50	0.024415087677836768	1.2400515714704239
>100	0.009526314692055218	1.2634012219768673
>500	4.265552694824725E-4	0.2047854391064468
>1k	4.265552668037909E-5	0.03489688396608555
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	3.118429858092731E-5	0.0	0.0	0.0	0.0
10-11	3.118429858092731E-5	0.0	0.0	0.0	0.0
12-13	3.118429858092731E-5	0.0	0.0	0.0	0.0
14-15	3.118429858092731E-5	0.0	0.0	0.0	0.0
16-17	3.118429858092731E-5	0.0	0.0	0.0	0.0
18-19	3.118429858092731E-5	0.0	0.0	0.0	0.0
20-21	3.118429858092731E-5	0.0	0.0	0.0	0.0
22-23	3.118429858092731E-5	0.0	0.0	0.0	0.0
24-25	4.677644787139096E-5	0.0	0.0	0.0	0.0
26-27	6.236859716185462E-5	0.0	0.0	0.0	0.0
28-29	6.236859716185462E-5	0.0	0.0	0.0	0.0
30-31	6.236859716185462E-5	0.0	0.0	0.0	0.0
32-33	6.236859716185462E-5	0.0	0.0	0.0	0.0
34-35	6.236859716185462E-5	0.0	0.0	0.0	0.0
36-37	6.236859716185462E-5	0.0	0.0	0.0	0.0
38-39	6.236859716185462E-5	0.0	0.0	0.0	0.0
40-41	6.236859716185462E-5	0.0	0.0	0.0	0.0
42-43	6.236859716185462E-5	0.0	0.0	0.0	0.0
44-45	6.236859716185462E-5	0.0	0.0	0.0	0.0
46-47	6.236859716185462E-5	0.0	0.0	0.0	0.0
48-49	6.236859716185462E-5	0.0	0.0	0.0	0.0
50-51	6.236859716185462E-5	0.0	0.0	0.0	0.0
52-53	9.355289574278192E-5	0.0	0.0	0.0	0.0
54-55	1.5592149290463654E-4	0.0	0.0	2.962508365188094E-4	0.0
56-57	2.026979407760275E-4	0.0	0.0	5.925016730376189E-4	0.0
58-59	2.4947438864741846E-4	0.0	0.0	5.925016730376189E-4	0.0
60-61	2.962508365188094E-4	0.0	0.0	5.925016730376189E-4	0.0
62-63	3.118429858092731E-4	0.0	0.0	5.925016730376189E-4	0.0
64-65	3.742115829711277E-4	0.0	0.0	5.925016730376189E-4	0.0
66-67	4.365801801329823E-4	0.0	0.0	5.925016730376189E-4	0.0
68-69	4.989487772948369E-4	0.0	0.0	5.925016730376189E-4	0.0
70-71	5.925016730376189E-4	0.0	0.0	5.925016730376189E-4	0.0
72-73	6.548702701994735E-4	0.0	0.0	5.925016730376189E-4	0.0
74-75	7.328310166517917E-4	0.0	0.0	5.925016730376189E-4	0.0
76-77	9.199368081373555E-4	0.0	0.0	5.925016730376189E-4	0.0
78-79	0.0012317797939466286	0.0	0.0	5.925016730376189E-4	0.0
80-81	0.00162158352620822	0.0	0.0	5.925016730376189E-4	0.0
82-83	0.0019178343627270294	0.0	0.0	5.925016730376189E-4	0.0
84-85	0.00208934800492213	0.0	0.0	5.925016730376189E-4	0.0
86-87	0.002120532303503057	6.236859716185462E-5	0.0	5.925016730376189E-4	0.0
88	0.002120532303503057	6.236859716185462E-5	0.0	5.925016730376189E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	11285	0.0	77.98597	8
CACCGGC	11395	0.0	75.54431	9
CTTCACC	12970	0.0	71.400925	6
GTGTGGC	5185	0.0	68.61787	7
CCTTCAC	14295	0.0	68.32894	5
CGGGGCA	1460	0.0	65.90491	94
CGTAGTG	2355	0.0	64.77526	4
TTCACCG	14265	0.0	64.19516	7
GGCAGCC	30	1.3806424E-4	62.58287	3
GGCTGCC	85	0.0	62.313995	1
CGGGCCC	950	0.0	61.265335	7
ACGGGCC	945	0.0	60.59611	6
CAGCAGC	160	0.0	59.587757	1
GGCGCGC	545	0.0	59.42502	6
GGCCCGA	950	0.0	59.289036	9
GGGCCCG	980	0.0	58.910915	8
TGTGGCA	5840	0.0	58.59107	8
GCCTTCA	4600	0.0	58.36533	4
ACCTTCA	5480	0.0	57.81493	4
AACGGGC	965	0.0	57.394653	5
>>END_MODULE