FastQCFastQC Report
Tue 7 Mar 2017
1NF_23A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_23A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3206742
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG112850.077.985978
CACCGGC113950.075.544319
CTTCACC129700.071.4009256
GTGTGGC51850.068.617877
CCTTCAC142950.068.328945
CGGGGCA14600.065.9049194
CGTAGTG23550.064.775264
TTCACCG142650.064.195167
GGCAGCC301.3806424E-462.582873
GGCTGCC850.062.3139951
CGGGCCC9500.061.2653357
ACGGGCC9450.060.596116
CAGCAGC1600.059.5877571
GGCGCGC5450.059.425026
GGCCCGA9500.059.2890369
GGGCCCG9800.058.9109158
TGTGGCA58400.058.591078
GCCTTCA46000.058.365334
ACCTTCA54800.057.814934
AACGGGC9650.057.3946535
Produced by FastQC (version 0.11.5)