FastQCFastQC Report
Tue 7 Mar 2017
1NF_24A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_24A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3038626
Sequences flagged as poor quality0
Sequence length95
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA174010.572660143104153No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA80640.2653831040740124No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA69220.2278003281746421No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT68090.22408154211804943No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG65680.21615032583805968No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG58200.19153393672008337No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG57320.1886378909414979No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG52610.17313746410384168No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT50560.16639099382418238No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA48220.1586901448220347No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT47700.15697884504377965No Hit
CAGCTCATCAACTTCAGATTCAGCCGCACCACATTGACGGGTACGAGGTA47600.15664974893257677No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC45350.14924508643051168No Hit
CAGCACATTTCAGGGTGGTGGATTTTCCGGGTCCATGGTTCATCCGGTGT43490.14312389876213788No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA41860.13775963214953074No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC38790.12765638153560196No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA35400.11650002336582391No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA34400.11320906225379497No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA33080.10886499358591678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTGCT355.5479177E-1089.000012
GCAGCAC355.5479177E-1089.000013
CAGCAGC4200.089.01
GCTGCTC202.4113573E-589.03
GCTGCCT158.5780147E-489.03
GCTGCCG158.5780147E-489.03
GCTGCAG301.9388608E-889.03
GCTGCAC202.4113573E-589.03
GCTGCAA600.089.03
TGCTGCC401.6370905E-1189.02
CTGCAGC1050.089.01
AGCTGCA1200.089.02
GCAGCTG401.6370905E-1189.03
GCAGCTA158.5780147E-489.03
AGCAGCG1350.089.02
CAGCTGC4900.088.0918351
AGCTGCT1800.086.527782
AGCTGCC1400.085.8214342
CTGCTGC1300.085.576931
GCTGCCC1100.084.954543
Produced by FastQC (version 0.11.5)