FastQCFastQC Report
Tue 7 Mar 2017
1NF_24A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_24A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3038626
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG120250.078.731698
CACCGGC114250.077.813069
CTTCACC133850.073.888176
ACGCAGC700.073.76772
AGGCTGC700.073.76772
CCTTCAC146500.071.6732565
GTGTGGC51000.071.051937
CGTAGTG23000.066.938314
TTCACCG149350.065.08847
GGCAGCG452.2244421E-762.5846023
GCCTTCA48200.062.2275284
TGTGGCA57100.062.0639768
ACCTTCA54500.061.6659244
CGGGGCA12500.061.58324494
AGGCAGC702.1827873E-1160.3553922
GCTGCCG408.0950285E-658.6788562
CGGGCCC8200.058.3868527
TGTGTGG59100.057.978126
ATGTGTG56050.057.4483075
GCTGCGT754.1836756E-1156.3317032
Produced by FastQC (version 0.11.5)