##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1NF_24A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3038626
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.823449151030761	12.0	12.0	12.0	2.0	32.0
2	14.557644474838298	12.0	12.0	12.0	12.0	22.0
3	13.727172083698356	12.0	12.0	12.0	12.0	22.0
4	13.825074885820104	12.0	12.0	12.0	12.0	22.0
5	22.43714330095247	22.0	22.0	22.0	22.0	27.0
6	26.845064183614568	27.0	22.0	32.0	22.0	32.0
7	34.96473669349239	37.0	37.0	37.0	27.0	41.0
8	37.58345745741661	41.0	37.0	41.0	27.0	41.0
9	38.55267380717469	41.0	41.0	41.0	32.0	41.0
10-11	38.911197363545234	41.0	41.0	41.0	37.0	41.0
12-13	39.08755684312581	41.0	41.0	41.0	37.0	41.0
14-15	39.03872358756885	41.0	41.0	41.0	37.0	41.0
16-17	38.93904745105189	41.0	41.0	41.0	37.0	41.0
18-19	38.87071854186728	41.0	41.0	41.0	37.0	41.0
20-21	38.88468422899034	41.0	41.0	41.0	37.0	41.0
22-23	38.87852075247167	41.0	41.0	41.0	37.0	41.0
24-25	38.89918914009161	41.0	41.0	41.0	37.0	41.0
26-27	38.86346114987498	41.0	41.0	41.0	37.0	41.0
28-29	38.819250378295976	41.0	41.0	41.0	37.0	41.0
30-31	38.692270618365015	41.0	41.0	41.0	32.0	41.0
32-33	38.727007864738866	41.0	41.0	41.0	32.0	41.0
34-35	38.70602222846773	41.0	41.0	41.0	32.0	41.0
36-37	38.63306754434406	41.0	41.0	41.0	32.0	41.0
38-39	38.52780911504081	41.0	41.0	41.0	32.0	41.0
40-41	38.57900396429176	41.0	41.0	41.0	32.0	41.0
42-43	38.310118948498435	41.0	41.0	41.0	32.0	41.0
44-45	38.4199295997599	41.0	41.0	41.0	32.0	41.0
46-47	38.436592394062316	41.0	41.0	41.0	32.0	41.0
48-49	38.37744954462971	41.0	41.0	41.0	32.0	41.0
50-51	38.255187871097	41.0	41.0	41.0	32.0	41.0
52-53	38.23915101759808	41.0	41.0	41.0	32.0	41.0
54-55	38.10779312755173	41.0	37.0	41.0	32.0	41.0
56-57	37.886794228707316	41.0	37.0	41.0	29.5	41.0
58-59	37.88324624353244	41.0	37.0	41.0	29.5	41.0
60-61	37.88303101467571	41.0	37.0	41.0	29.5	41.0
62-63	37.88611546797796	41.0	37.0	41.0	29.5	41.0
64-65	37.866421533943296	41.0	37.0	41.0	29.5	41.0
66-67	37.72784014880409	41.0	37.0	41.0	27.0	41.0
68-69	37.76005141797641	41.0	37.0	41.0	27.0	41.0
70-71	37.74792471992275	41.0	37.0	41.0	27.0	41.0
72-73	37.58700544259149	41.0	37.0	41.0	27.0	41.0
74-75	37.48331943450757	41.0	37.0	41.0	27.0	41.0
76-77	36.431417357713656	39.0	34.5	41.0	24.5	41.0
78-79	36.95399565461495	41.0	37.0	41.0	27.0	41.0
80-81	37.61819881749185	41.0	37.0	41.0	27.0	41.0
82-83	37.804884674849745	41.0	37.0	41.0	27.0	41.0
84-85	37.73295545420858	41.0	37.0	41.0	27.0	41.0
86-87	37.58448078177439	41.0	37.0	41.0	27.0	41.0
88-89	37.493026453403616	41.0	37.0	41.0	27.0	41.0
90-91	37.333735214534464	41.0	37.0	41.0	27.0	41.0
92-93	37.23030606596534	41.0	37.0	41.0	27.0	41.0
94-95	37.07109726567205	41.0	37.0	41.0	27.0	41.0
96-97	37.00735348805677	41.0	37.0	41.0	27.0	41.0
98-99	36.89134743795387	41.0	37.0	41.0	27.0	41.0
100	36.75240651531317	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.5550954507704926
1101	2	-0.44398126927563375
1101	3	0.02544684239256334
1101	4	-0.006415333469469431
1101	5	-1.1004970737093345
1101	6	-0.9191997364671316
1101	7	-0.5232205158276884
1101	8	-0.21626247458591763
1101	9	0.02483667114704957
1101	10-11	-0.10432749724201784
1101	12-13	-0.1992620036542121
1101	14-15	-0.13357713061039078
1101	16-17	-0.10558239323315632
1101	18-19	-0.07420952769663103
1101	20-21	-0.02873002431203986
1101	22-23	0.04576894813165211
1101	24-25	-0.11167315346343543
1101	26-27	0.0336965144353627
1101	28-29	-0.04899772981455186
1101	30-31	-0.1732201061045231
1101	32-33	-0.180836122749092
1101	34-35	-0.12350250341181379
1101	36-37	-0.05722235903377282
1101	38-39	-0.13469336054571102
1101	40-41	-0.13919280209459828
1101	42-43	-0.07786064909245738
1101	44-45	-0.04625501310182756
1101	46-47	-0.07019820175277403
1101	48-49	-0.08812124587563375
1101	50-51	-0.15928484344090776
1101	52-53	-0.10665712859165666
1101	54-55	-0.09128124290042194
1101	56-57	0.13519326265197407
1101	58-59	-0.04193476628640269
1101	60-61	-0.21322496954345382
1101	62-63	-0.22857125017842606
1101	64-65	-0.0012151703894218713
1101	66-67	-0.0661441977701358
1101	68-69	-0.0672381039649963
1101	70-71	-0.11926074503504935
1101	72-73	-0.04503411056516171
1101	74-75	0.057206654201586105
1101	76-77	-0.3501712987291512
1101	78-79	-0.1054221092092078
1101	80-81	-0.1566098154317359
1101	82-83	-0.13232926106016407
1101	84-85	-0.14718504471827742
1101	86-87	-0.2246122456619304
1101	88-89	-0.40287559665634376
1101	90-91	-0.23481346188929564
1101	92-93	-0.11547147962016169
1101	94-95	0.004693953362973957
1101	96-97	-0.23136246905409763
1101	98-99	-0.5599055576316587
1101	100	-0.6301433110172567
1102	1	1.6028424136977257
1102	2	0.7342068341452794
1102	3	-0.11169391187014988
1102	4	0.13552264087281785
1102	5	-0.7911061792793603
1102	6	-0.17034550417827887
1102	7	-0.03490356941559014
1102	8	-0.16814944814437638
1102	9	-0.2800234076238297
1102	10-11	-0.22253593444465736
1102	12-13	-0.3026445220076397
1102	14-15	-0.14802575870385937
1102	16-17	-0.2794569489081411
1102	18-19	-0.18069194285646262
1102	20-21	-0.09691964548967036
1102	22-23	-0.12296353096107282
1102	24-25	-0.07951625484334812
1102	26-27	-0.1547325657832772
1102	28-29	-0.28570672040931555
1102	30-31	-0.21137754945373644
1102	32-33	-0.2270170934064879
1102	34-35	-0.1304584725271951
1102	36-37	-0.2535964451744377
1102	38-39	-0.15527726026564892
1102	40-41	-0.21600619217402084
1102	42-43	-0.05310194815359637
1102	44-45	0.005385515576492139
1102	46-47	-0.3827245459203965
1102	48-49	-0.2961985530564206
1102	50-51	-0.26990255656271245
1102	52-53	-0.2338554675143456
1102	54-55	-0.235888182028674
1102	56-57	-0.09929599142257928
1102	58-59	-0.11755959345965294
1102	60-61	-0.20703085885532602
1102	62-63	-0.18422510184993257
1102	64-65	-0.2776463491319632
1102	66-67	-0.2616551030946894
1102	68-69	-0.29943402071430825
1102	70-71	-0.38379028550129135
1102	72-73	-0.23729985798410524
1102	74-75	-0.34607121517293393
1102	76-77	-0.2784116226835849
1102	78-79	-0.2251560651693154
1102	80-81	-0.11025482253192109
1102	82-83	-0.2577071235735886
1102	84-85	-0.22969605191965314
1102	86-87	-0.033784555403499894
1102	88-89	-0.10225179171649756
1102	90-91	-0.3033696317089465
1102	92-93	-0.19167402460016802
1102	94-95	-0.2774753160138914
1102	96-97	-0.2857273363820738
1102	98-99	-0.15116258591945808
1102	100	-0.6105201720884565
1103	1	1.650952883339082
1103	2	0.6175069737275489
1103	3	0.4660223085493058
1103	4	1.1304611769755564
1103	5	2.870083637469996
1103	6	-0.2049891552414529
1103	7	-0.8113063112489414
1103	8	-0.48278136595678234
1103	9	-0.5324228259881991
1103	10-11	-0.48355627883899643
1103	12-13	-0.5833147054125121
1103	14-15	-0.5395530263730706
1103	16-17	-0.5686726547839953
1103	18-19	-0.45259753360192434
1103	20-21	-0.2834661225011459
1103	22-23	-0.3592867820197938
1103	24-25	-0.4819803945958441
1103	26-27	-0.4187389910804882
1103	28-29	-0.46449512802609405
1103	30-31	-0.4617283616557444
1103	32-33	-0.7487614519207071
1103	34-35	-0.5507733337975012
1103	36-37	-0.5676950398977354
1103	38-39	-0.45243968724345507
1103	40-41	-0.58088337148682
1103	42-43	-0.474177557073034
1103	44-45	-0.7349330530354621
1103	46-47	-0.698190401389482
1103	48-49	-0.5052942644504483
1103	50-51	-0.48945487078076866
1103	52-53	-0.3228365226557699
1103	54-55	-0.48505545825504726
1103	56-57	-0.5782109142153331
1103	58-59	-0.7039437957285628
1103	60-61	-0.40697831914064153
1103	62-63	-0.3918463737651692
1103	64-65	-0.5518913781380448
1103	66-67	-0.6964806509629611
1103	68-69	-0.6508098928892778
1103	70-71	-0.6451665118444936
1103	72-73	-0.5062146551042588
1103	74-75	-0.5562808427793158
1103	76-77	-0.7382453035682914
1103	78-79	-0.8680303145968651
1103	80-81	-0.41378813619559196
1103	82-83	-0.4884622216681933
1103	84-85	-0.7309611322744232
1103	86-87	-0.4899741593663052
1103	88-89	-0.5370499915136477
1103	90-91	-0.8803192492786636
1103	92-93	-0.6115875904144801
1103	94-95	-0.561323445248739
1103	96-97	-0.4773921155795975
1103	98-99	-0.5330630469379614
1103	100	-0.5926265362345262
1104	1	1.850773501885083
1104	2	-0.5610366748946856
1104	3	-0.08904681760181177
1104	4	0.1106190857026288
1104	5	-1.0724479878090065
1104	6	-0.5172351252130802
1104	7	-0.1760145814201337
1104	8	-0.08918380199267517
1104	9	-0.15422836865025147
1104	10-11	-0.10519157921788036
1104	12-13	-0.03601200050624698
1104	14-15	-0.19050055946004107
1104	16-17	-0.14199835580452458
1104	18-19	-0.11774451280324882
1104	20-21	-0.0774364896767068
1104	22-23	-0.10480657019860473
1104	24-25	-0.1844245606047039
1104	26-27	-0.08884188026934225
1104	28-29	-0.05757167276219377
1104	30-31	-0.23432676342522285
1104	32-33	-0.19811069682914706
1104	34-35	-0.04416631871988841
1104	36-37	-0.09918049247119853
1104	38-39	0.06808755353996787
1104	40-41	0.0121303234309309
1104	42-43	-0.06360679423192295
1104	44-45	-0.04605522212683866
1104	46-47	-0.34641146322851313
1104	48-49	-0.29588126445563745
1104	50-51	-0.20656409050315716
1104	52-53	-0.08928587694596501
1104	54-55	-0.06660929604370125
1104	56-57	-0.19450801626209824
1104	58-59	-0.10970525010795029
1104	60-61	-0.20022764321679176
1104	62-63	-0.2879507229149354
1104	64-65	-0.3320334879199578
1104	66-67	-0.7615293480400922
1104	68-69	-0.5036693866426489
1104	70-71	-0.30151278666656367
1104	72-73	-0.2036149790402959
1104	74-75	-0.273470354586145
1104	76-77	-0.3063733534422255
1104	78-79	-0.25630899645742744
1104	80-81	-0.3322831655822043
1104	82-83	-0.19262654107673427
1104	84-85	-0.19888249850261985
1104	86-87	-0.27435719037662665
1104	88-89	-0.33241199205219374
1104	90-91	-0.26508396319955096
1104	92-93	-0.4308558743780324
1104	94-95	-0.24167287294059747
1104	96-97	-0.1982476745883659
1104	98-99	-0.22025950105499703
1104	100	-0.37577246371044737
1105	1	1.5068767324831356
1105	2	0.41141011253270143
1105	3	-0.058339639418459655
1105	4	-0.3995022615051518
1105	5	-1.1460020681599445
1105	6	0.40582501360365697
1105	7	-0.07484141294800395
1105	8	-0.28007404749166653
1105	9	-0.24798289411976526
1105	10-11	-0.2442219244974808
1105	12-13	-0.2901238021524719
1105	14-15	-0.24305222242850277
1105	16-17	-0.23000280754623503
1105	18-19	-0.21999726199558012
1105	20-21	-0.17191522924675695
1105	22-23	-0.2076374676653998
1105	24-25	-0.197448114865729
1105	26-27	-0.19023261872311537
1105	28-29	-0.14179592424779486
1105	30-31	-0.0863763963427644
1105	32-33	-0.14246927606311743
1105	34-35	-0.06475895961504108
1105	36-37	-0.23736091809394821
1105	38-39	-0.1747527193793701
1105	40-41	-0.020649780374476734
1105	42-43	-0.03615156111447959
1105	44-45	-0.16285771194462484
1105	46-47	-0.3202257936897297
1105	48-49	-0.10585469743059406
1105	50-51	-0.1942712128173696
1105	52-53	-0.40596805355191634
1105	54-55	-0.06890965908189628
1105	56-57	-0.08868895387962539
1105	58-59	-0.15082681050790825
1105	60-61	-0.2734202419182381
1105	62-63	-0.3947114998906258
1105	64-65	-0.3990824273375253
1105	66-67	-0.32124601925134044
1105	68-69	-0.30593357372030283
1105	70-71	-0.5688049097020809
1105	72-73	-0.44590816525712995
1105	74-75	-0.48014766140470755
1105	76-77	-0.35540095251751325
1105	78-79	-0.25767007701654876
1105	80-81	-0.5446960924217095
1105	82-83	-0.4183125570875461
1105	84-85	-0.41498515095239696
1105	86-87	-0.4270591441008591
1105	88-89	-0.15796791080346395
1105	90-91	-0.3442821465909631
1105	92-93	-0.20955440424146587
1105	94-95	-0.19367801901615422
1105	96-97	-0.3272579875861723
1105	98-99	-0.41649775707072223
1105	100	-0.4686389200756764
1106	1	1.5636138666759472
1106	2	-0.7088860849373901
1106	3	-0.06327494035474857
1106	4	-0.2583426595276901
1106	5	-1.1462656402032891
1106	6	-0.5459738895532666
1106	7	0.10942748475674335
1106	8	-0.08677414467786804
1106	9	-0.19124466456261757
1106	10-11	-0.1216559952027012
1106	12-13	-0.14012248771526714
1106	14-15	-0.07713724953739387
1106	16-17	0.02803791974282177
1106	18-19	0.09668050892704372
1106	20-21	0.057995788831412654
1106	22-23	0.012269519472013712
1106	24-25	-0.06709599356945972
1106	26-27	-0.05082457277916319
1106	28-29	-0.10398107203846507
1106	30-31	0.008960897828011127
1106	32-33	-0.07989248075412547
1106	34-35	-0.0053014880938278
1106	36-37	-0.18317940581751913
1106	38-39	0.03171079280231481
1106	40-41	-0.11833026424071846
1106	42-43	0.15325569069033662
1106	44-45	-0.011579271151944681
1106	46-47	-0.15826090997497744
1106	48-49	0.01231115332655719
1106	50-51	-0.006047707558387572
1106	52-53	-0.13752184429296932
1106	54-55	-0.15641824742289856
1106	56-57	-0.04194120012957114
1106	58-59	-0.06802034742018037
1106	60-61	-0.05039358869169064
1106	62-63	-0.0255387354676202
1106	64-65	-0.2334690219401736
1106	66-67	-0.1064370631143774
1106	68-69	-0.11220059123217396
1106	70-71	-0.3503756375141762
1106	72-73	-0.13330336969729473
1106	74-75	-0.2508669570416231
1106	76-77	-0.1871605211411378
1106	78-79	0.05347363943963046
1106	80-81	-0.07649328681026901
1106	82-83	-0.1561329956549926
1106	84-85	-0.186696572836631
1106	86-87	-0.21789165659554754
1106	88-89	-0.31401645789851784
1106	90-91	-0.3494650683274543
1106	92-93	-0.20380531606382135
1106	94-95	-0.13172537599785272
1106	96-97	-0.05016942217663001
1106	98-99	-0.3763433435602863
1106	100	-0.21908384337690023
1107	1	1.1193412608263582
1107	2	-0.507295980635039
1107	3	-0.1458753177564116
1107	4	-0.3434730615886501
1107	5	-1.242024075571532
1107	6	-0.5065367941952523
1107	7	-0.36940301117219576
1107	8	-0.18534999786338346
1107	9	-0.1665972208455031
1107	10-11	-0.038033270053936974
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2224	5	-1.037309928608451
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2224	20-21	0.09981511963196965
2224	22-23	0.12561152191928215
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2224	32-33	0.1557836953778775
2224	34-35	0.13490434925860484
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2224	44-45	0.23647413538326134
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2224	48-49	0.10403612904025294
2224	50-51	0.1533275447951894
2224	52-53	0.21449848671981186
2224	54-55	0.14334098211161006
2224	56-57	0.41167490424748365
2224	58-59	0.32629840053584047
2224	60-61	0.23107089508242495
2224	62-63	0.29330251592174506
2224	64-65	0.2660877076808603
2224	66-67	0.29594398763695295
2224	68-69	0.36582248055844957
2224	70-71	0.3574473435431784
2224	72-73	0.3312368779653525
2224	74-75	0.30404155983966774
2224	76-77	0.5067606450094004
2224	78-79	0.2701378155469456
2224	80-81	0.2544329678615398
2224	82-83	0.26403111834483184
2224	84-85	0.2255362945519508
2224	86-87	0.32283180573444525
2224	88-89	0.12493087790498691
2224	90-91	0.274869087114034
2224	92-93	0.2089283683802492
2224	94-95	0.4621109188654131
2224	96-97	0.24148974374801213
2224	98-99	0.37786809392886767
2224	100	0.6822571417417223
2225	1	1.3801280511664054
2225	2	1.467858798743361
2225	3	0.9279977594002471
2225	4	1.1004081972267628
2225	5	3.0894381895128475
2225	6	-0.17432282641829389
2225	7	0.015573170579358475
2225	8	0.15059477901070295
2225	9	0.141180131586097
2225	10-11	0.09985954680450959
2225	12-13	0.22823737709179426
2225	14-15	0.14578666524255368
2225	16-17	0.11742219299941326
2225	18-19	0.0054528065804220205
2225	20-21	0.11529296348780349
2225	22-23	0.15754917199112128
2225	24-25	0.17851479268090742
2225	26-27	0.16257278851206536
2225	28-29	0.13776838597195962
2225	30-31	0.16036761608483374
2225	32-33	0.05590191336621331
2225	34-35	0.09463778303495474
2225	36-37	0.14744685015670456
2225	38-39	0.1387413598483178
2225	40-41	0.08900553386928323
2225	42-43	0.32488242430432734
2225	44-45	0.07051970741502345
2225	46-47	0.09921099241184095
2225	48-49	0.15105723097109802
2225	50-51	0.12761310691369232
2225	52-53	0.17377430890326906
2225	54-55	0.04657342927709607
2225	56-57	0.22200243620891058
2225	58-59	0.20174935481306022
2225	60-61	0.10691378429234533
2225	62-63	0.10634198261399064
2225	64-65	0.25677211426202007
2225	66-67	0.26733632353595027
2225	68-69	0.2651072889559245
2225	70-71	0.24391285147611086
2225	72-73	0.24038274801906567
2225	74-75	0.37623634632146974
2225	76-77	0.39589007531185416
2225	78-79	0.2711066292936479
2225	80-81	0.08458807767171805
2225	82-83	0.014579143257073213
2225	84-85	0.12670440940336647
2225	86-87	0.2625948199066599
2225	88-89	0.21257900400842544
2225	90-91	0.3349449028161615
2225	92-93	0.26208907591378505
2225	94-95	0.5059199739037794
2225	96-97	0.35540016662734786
2225	98-99	0.2964271758953245
2225	100	0.2225508020779685
2226	1	1.2658440217246678
2226	2	1.304580910500997
2226	3	0.792247622369116
2226	4	0.9853684545464567
2226	5	3.1779854682744784
2226	6	-0.3467680873458896
2226	7	0.22124577461975292
2226	8	0.2521496224751303
2226	9	0.3267996391346699
2226	10-11	0.20278935007602428
2226	12-13	0.19030429131542093
2226	14-15	0.19064608199396815
2226	16-17	0.11825434085464082
2226	18-19	0.19966679242853047
2226	20-21	0.2496882620748906
2226	22-23	0.19918890502911069
2226	24-25	0.27669462613446427
2226	26-27	0.24503006780361147
2226	28-29	0.2977325579924752
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2226	32-33	0.24585256090886531
2226	34-35	0.17042446524266097
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2226	60-61	0.33346306615889887
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2226	68-69	0.436681487445
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2226	74-75	0.5166185804993333
2226	76-77	0.6258255235885954
2226	78-79	0.5340015342856219
2226	80-81	0.4818704452084077
2226	82-83	0.35228773306031513
2226	84-85	0.4326765627650744
2226	86-87	0.40383941718787497
2226	88-89	0.43011391523735654
2226	90-91	0.34373461810352524
2226	92-93	0.33904797693407573
2226	94-95	0.6418861997642935
2226	96-97	0.5423233791231539
2226	98-99	0.45996305587159725
2226	100	0.7402491678381296
2227	1	1.220826215726051
2227	2	1.775611874215116
2227	3	0.6902554285360036
2227	4	-0.18231202199671515
2227	5	3.07759184793154
2227	6	0.01281359121236747
2227	7	0.3098895459390789
2227	8	0.20914492397784556
2227	9	0.35394280267554734
2227	10-11	0.3296894423044918
2227	12-13	0.22504234828873848
2227	14-15	0.2312201341413882
2227	16-17	0.18912523354241983
2227	18-19	0.2450739585784092
2227	20-21	0.15991407093036258
2227	22-23	0.3441033312667656
2227	24-25	0.2913780645124646
2227	26-27	0.24123903140715441
2227	28-29	0.17363037001679515
2227	30-31	0.21903441524762002
2227	32-33	0.27245058017347645
2227	34-35	0.1306371462974667
2227	36-37	0.06445083287136555
2227	38-39	0.19001938487117798
2227	40-41	0.3064538826588432
2227	42-43	0.3928969509920748
2227	44-45	0.2790001298976392
2227	46-47	0.17482937441274515
2227	48-49	0.1628905434668937
2227	50-51	0.28532384123294463
2227	52-53	0.23266192743756875
2227	54-55	0.32255322325822533
2227	56-57	0.47286714893580495
2227	58-59	0.5029411718452792
2227	60-61	0.3685111555764564
2227	62-63	0.5693441728505135
2227	64-65	0.412317117533469
2227	66-67	0.4762662157145314
2227	68-69	0.24099652741735156
2227	70-71	0.45029795397923067
2227	72-73	0.3824854479966291
2227	74-75	0.3543471451245708
2227	76-77	0.5869564161748713
2227	78-79	0.6007258181527391
2227	80-81	0.5032317674223279
2227	82-83	0.3469781853965088
2227	84-85	0.30472998583575617
2227	86-87	0.4666858582451212
2227	88-89	0.3545873275013136
2227	90-91	0.41420967354611093
2227	92-93	0.4504034443892806
2227	94-95	0.48341982336045675
2227	96-97	0.4442503613075388
2227	98-99	0.6196225530407773
2227	100	0.5172579149865371
2228	1	1.454055894423675
2228	2	1.6495378745228386
2228	3	-0.10227738868362302
2228	4	-0.05450504636365139
2228	5	3.2511512779390195
2228	6	0.25107672822274196
2228	7	0.18810614633696332
2228	8	0.3177277437618429
2228	9	0.3218776190259831
2228	10-11	0.242893531263924
2228	12-13	0.20504253724250532
2228	14-15	0.1801489012720694
2228	16-17	0.1846113980779549
2228	18-19	-0.011905723493391918
2228	20-21	0.15942967446218148
2228	22-23	0.2326210159893094
2228	24-25	0.22775017925598462
2228	26-27	0.18637009783520142
2228	28-29	0.14236351601596908
2228	30-31	0.10804230001821935
2228	32-33	0.06202613603495877
2228	34-35	0.1690650779825802
2228	36-37	0.2412991380910725
2228	38-39	0.05314505801896274
2228	40-41	0.020259384452558038
2228	42-43	0.2995566263695153
2228	44-45	0.1765681600006701
2228	46-47	0.004321412710979189
2228	48-49	-0.07002007330518722
2228	50-51	-0.05440220280407715
2228	52-53	0.1705063968958953
2228	54-55	0.10127406026940378
2228	56-57	0.3256203402298894
2228	58-59	0.384315054656156
2228	60-61	0.24775687914789302
2228	62-63	0.25121162988377677
2228	64-65	0.2911518666703543
2228	66-67	0.29370907744626606
2228	68-69	0.40666862245569746
2228	70-71	0.4713822246581998
2228	72-73	0.47633716248209623
2228	74-75	0.39368218983913295
2228	76-77	0.37763478717748455
2228	78-79	0.3169897433702431
2228	80-81	0.23267845681743182
2228	82-83	0.23002160967059382
2228	84-85	0.2864562674146569
2228	86-87	0.2946973088431619
2228	88-89	0.3930817979044079
2228	90-91	0.49568667112480824
2228	92-93	0.46465109798844395
2228	94-95	0.7740168092780095
2228	96-97	0.5105738069603873
2228	98-99	0.6174124688483786
2228	100	0.5613826676595579
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	21.0
13	40.0
14	135.0
15	511.0
16	1913.0
17	4809.0
18	8047.0
19	10428.0
20	12019.0
21	14083.0
22	16045.0
23	18667.0
24	21102.0
25	23750.0
26	27232.0
27	30494.0
28	34625.0
29	39752.0
30	45308.0
31	52478.0
32	61943.0
33	73792.0
34	90586.0
35	116395.0
36	164714.0
37	273346.0
38	667868.0
39	1227174.0
40	1349.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.07923950207136	18.486211141307272	19.566964038858416	34.86758531776295
2	20.29045129678217	51.22585770874829	11.723434758053793	16.760256236415742
3	39.41524886577025	15.387579781124758	24.717026708782193	20.480144644322795
4	18.619171954692682	15.915548672327558	22.194439197189784	43.27084017578998
5	17.645705657754522	35.08773373228558	26.562301513907933	20.704259096051967
6	17.05241118847795	27.57697064396869	31.466886678386874	23.903731489166486
7	20.56617036779123	26.88310440310851	36.2658319911697	16.284893237930564
8	20.123898103945663	27.826688773149442	34.91008765145826	17.139325471446636
9	20.56340596045713	28.345245515571843	27.95414769701832	23.137200826952707
10-11	19.165027219539358	30.61418219945462	28.984366618333418	21.236423962672603
12-13	23.997194784748107	26.569327715882114	27.539058771958114	21.894418727411665
14-15	21.889712653021466	27.375695462356997	28.56165911829886	22.172932766322674
16-17	20.97123831626531	29.723368390845074	28.250449380739845	21.054943912149767
18-19	21.475940112406068	29.517814959787746	29.91786419256598	19.088380735240204
20-21	22.755712614846317	28.966003055328297	29.883012914389596	18.395271415435793
22-23	22.127665596226713	30.23736715212731	28.713421789980075	18.921545461665897
24-25	22.176519913934786	28.25059747398989	26.617984575923458	22.954898036151867
26-27	19.979359091905355	26.408449081920576	30.169359440747236	23.442832385426833
28-29	19.418623095924655	28.29584475027101	30.75579853183063	21.5297336219737
30-31	20.13724392450679	30.022466414750554	28.358467346846755	21.4818223138959
32-33	20.352998361759557	30.32872423259723	28.892417164863332	20.425860240779876
34-35	21.19632360152253	29.40997016414656	29.932870975236835	19.46083525909408
36-37	21.877355094045793	28.057253508658192	28.900990118560166	21.164401278735852
38-39	21.64084770785436	29.077922223238772	29.042692113523554	20.23853795538331
40-41	20.979402207216665	29.723492370444333	29.812283903852393	19.48482151848661
42-43	20.367591027707793	30.025885092960742	29.548053286663045	20.058470592668417
44-45	20.68892321726991	29.96021886207779	28.86742231521747	20.483435605434824
46-47	20.064020225296694	28.151061798457235	28.91291663087682	22.872001345369252
48-49	20.696624066272058	27.9443735425156	30.596493283477464	20.76250910773488
50-51	21.634712531255904	30.61953001126167	28.39880590767011	19.346951549812317
52-53	20.519870011928244	30.22726191931676	28.34008510206966	20.91278296668533
54-55	22.408254691362494	28.33499010872177	28.30281465291653	20.953940546999206
56-57	21.77451233050838	28.55648118983226	29.42208692624142	20.246919553417943
58-59	21.237612332216518	28.14696008743181	29.09345780396534	21.521969776386335
60-61	19.58653249823892	30.48409992896443	29.586636163769818	20.342731409026833
62-63	20.57046767804034	31.172068471130032	27.395166418497002	20.862297432332625
64-65	19.588053468306775	30.28905367481482	28.997580318055306	21.125312538823103
66-67	20.18703519287994	30.298463845172126	29.582103885111234	19.932397076836704
68-69	20.828213146336534	29.99066025236406	29.36017792252156	19.820948678777842
70-71	19.581284435794334	31.068055101220093	29.227601554123478	20.1230589088621
72-73	20.016514042860162	30.723820568901868	28.566957565689226	20.692707822548744
74-75	20.090840399575335	29.20064858261596	30.59068473711474	20.117826280693972
76-77	19.593987545686765	30.46469029094071	28.69456458280815	21.246757580564374
78-79	19.57034199009684	29.508995184007507	28.582392173304644	22.338270652591007
80-81	20.48188290613311	29.492974117873167	28.64612643494321	21.379016541050515
82-83	20.241763876172982	28.557232775603186	29.978615334694037	21.222388013529798
84-85	20.43074731803124	29.0574917742427	28.39316190936298	22.118598998363076
86-87	21.465622949319858	29.07414403746957	29.071593542607744	20.388639470602833
88-89	21.00139997485706	28.778138540248126	28.674456810413655	21.546004674481164
90-91	20.467573172874847	29.392577434669487	27.873979884329298	22.26586950812637
92-93	20.25772503756632	29.40542863781196	28.773597013913527	21.563249310708198
94-95	20.292115581187023	29.43062094512454	28.347878284461466	21.929385189226974
96-97	20.11007606727514	29.396625316837284	28.499953597448318	21.993345018439257
98-99	21.113440745916083	29.787640861362995	27.79399307450144	21.304925318219485
100	21.111285166387702	31.106987171175394	26.541305182013186	21.240422480423717
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	10.0
2	17.5
3	19.0
4	23.0
5	30.0
6	37.5
7	50.5
8	59.0
9	72.0
10	87.0
11	88.0
12	93.5
13	112.5
14	136.0
15	200.5
16	291.5
17	377.5
18	552.0
19	875.5
20	1362.0
21	2059.0
22	3185.5
23	5022.5
24	7736.5
25	11524.0
26	16642.5
27	22562.5
28	29014.5
29	36353.5
30	44947.5
31	54906.5
32	66235.0
33	78255.0
34	90980.5
35	105336.5
36	119828.5
37	130573.0
38	137620.5
39	143539.0
40	146273.5
41	146361.0
42	145171.0
43	143113.5
44	141691.5
45	137620.0
46	127819.0
47	116521.5
48	108917.0
49	104621.0
50	100740.5
51	94244.0
52	83297.0
53	70372.5
54	58698.0
55	47545.5
56	36476.5
57	26872.0
58	19343.5
59	14085.0
60	10816.0
61	8780.5
62	7271.0
63	5679.0
64	4167.5
65	3262.5
66	3093.5
67	2852.5
68	2235.0
69	1976.0
70	2078.0
71	1906.5
72	1291.5
73	711.5
74	422.0
75	277.5
76	184.5
77	128.0
78	113.0
79	95.5
80	68.5
81	50.5
82	39.5
83	29.0
84	25.0
85	22.5
86	20.0
87	17.0
88	14.0
89	16.0
90	11.5
91	10.5
92	12.0
93	13.5
94	16.5
95	14.5
96	18.0
97	20.5
98	41.5
99	100.0
100	94.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.321663146435263
2	0.005989549223892642
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	3.9491533344347083E-4
30-31	0.010300708280650531
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0010531075558492557
40-41	0.0015796613337738833
42-43	1.4809325004130157E-4
44-45	0.0
46-47	0.001810028611615908
48-49	0.0
50-51	0.0
52-53	0.0012999296392514248
54-55	0.019614128227692387
56-57	0.011485454280980944
58-59	0.012258830142307742
60-61	2.468220834021693E-4
62-63	0.012571471447950488
64-65	1.1518363892101234E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.3163844448115696E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3038626.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.6994576076269
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.88991660828688	63.30040267995049
2	8.694745453282742	12.815960478866362
3	2.437257171950009	5.388735948690428
4	1.0517151241240899	3.1004333682273364
5	0.5551632319157604	2.045761453794436
6	0.33102109370552907	1.4637645037668556
7	0.22887421833356889	1.180753402608752
8	0.15524652200575717	0.9153267573835852
9	0.11268313645048258	0.7474217434134101
>10	0.5084480277905123	6.343935804199277
>50	0.02463808788710112	1.2275229962751681
>100	0.00992861656615316	1.2753431416028354
>500	3.173692388517597E-4	0.15671260305433074
>1k	4.533846260564245E-5	0.03792511816677459
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.2909611120289233E-5	0.0
3	0.0	0.0	0.0	3.2909611120289233E-5	0.0
4	0.0	0.0	0.0	3.2909611120289233E-5	0.0
5	0.0	0.0	0.0	3.2909611120289233E-5	0.0
6	0.0	0.0	0.0	3.2909611120289233E-5	0.0
7	0.0	0.0	0.0	3.2909611120289233E-5	0.0
8	0.0	0.0	0.0	3.2909611120289233E-5	0.0
9	0.0	0.0	0.0	3.2909611120289233E-5	0.0
10-11	6.581922224057847E-5	0.0	0.0	3.2909611120289233E-5	0.0
12-13	9.872883336086771E-5	0.0	0.0	3.2909611120289233E-5	0.0
14-15	9.872883336086771E-5	0.0	0.0	3.2909611120289233E-5	0.0
16-17	9.872883336086771E-5	0.0	0.0	3.2909611120289233E-5	0.0
18-19	9.872883336086771E-5	0.0	0.0	3.2909611120289233E-5	0.0
20-21	9.872883336086771E-5	0.0	0.0	3.2909611120289233E-5	0.0
22-23	9.872883336086771E-5	4.9364416680433854E-5	0.0	3.2909611120289233E-5	0.0
24-25	9.872883336086771E-5	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
26-27	9.872883336086771E-5	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
28-29	9.872883336086771E-5	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
30-31	9.872883336086771E-5	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
32-33	9.872883336086771E-5	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
34-35	9.872883336086771E-5	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
36-37	9.872883336086771E-5	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
38-39	9.872883336086771E-5	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
40-41	9.872883336086771E-5	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
42-43	9.872883336086771E-5	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
44-45	1.1518363892101233E-4	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
46-47	1.3163844448115693E-4	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
48-49	1.3163844448115693E-4	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
50-51	1.3163844448115693E-4	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
52-53	2.468220834021693E-4	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
54-55	3.1264130564274777E-4	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
56-57	3.2909611120289235E-4	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
58-59	3.9491533344347083E-4	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
60-61	3.9491533344347083E-4	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
62-63	4.442797501239047E-4	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
64-65	4.607345556840493E-4	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
66-67	4.607345556840493E-4	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
68-69	5.100989723644832E-4	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
70-71	6.08827805725351E-4	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
72-73	7.733758613267971E-4	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
74-75	9.214691113680986E-4	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
76-77	0.0010531075558492557	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
78-79	0.0012176556114507017	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
80-81	0.0014973873059731602	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
82-83	0.0016454805560144618	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
84-85	0.001777119000495619	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
86-87	0.0018758478338564864	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
88	0.0018758478338564864	9.872883336086771E-5	0.0	3.2909611120289233E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	12025	0.0	78.73169	8
CACCGGC	11425	0.0	77.81306	9
CTTCACC	13385	0.0	73.88817	6
ACGCAGC	70	0.0	73.7677	2
AGGCTGC	70	0.0	73.7677	2
CCTTCAC	14650	0.0	71.673256	5
GTGTGGC	5100	0.0	71.05193	7
CGTAGTG	2300	0.0	66.93831	4
TTCACCG	14935	0.0	65.0884	7
GGCAGCG	45	2.2244421E-7	62.584602	3
GCCTTCA	4820	0.0	62.227528	4
TGTGGCA	5710	0.0	62.063976	8
ACCTTCA	5450	0.0	61.665924	4
CGGGGCA	1250	0.0	61.583244	94
AGGCAGC	70	2.1827873E-11	60.355392	2
GCTGCCG	40	8.0950285E-6	58.678856	2
CGGGCCC	820	0.0	58.386852	7
TGTGTGG	5910	0.0	57.97812	6
ATGTGTG	5605	0.0	57.448307	5
GCTGCGT	75	4.1836756E-11	56.331703	2
>>END_MODULE