FastQCFastQC Report
Tue 7 Mar 2017
1NF_25A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_25A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2203229
Sequences flagged as poor quality0
Sequence length95
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA138610.6291220749182224No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA59780.2713290357016906No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA56300.2555340366344125No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG49950.22671270212946543No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT48320.21931446980772312No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG46680.21187084955762658No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG41670.18913149745214863No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG41530.18849606645518918No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT39470.17914615321421423No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC37660.17093093818209545No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA35850.1627157231499767No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA33610.15254882719862528No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT33560.15232188755685405No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA30850.14002175897285302No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA30830.13993098311614452No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA30130.13675382813134723No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC28920.13126188880048328No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC26090.1184171050762313No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTTAAAAAAAAGCCATGAGTGCTC23560.10693395920260672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC700.089.000013
GCTGCCA355.5479177E-1089.000013
GCTGCAA355.5479177E-1089.000013
TGCTGCC401.6370905E-1189.000012
GCAGCTA202.4112087E-589.000013
GCTGCTA500.089.03
GCTGCCT450.089.03
GCTGCAT158.5777004E-489.03
GCTGCAC450.089.03
AGCTGCC1200.089.02
AGCTGCA900.089.02
GCAGCTT158.5777004E-489.03
GCAGCTC158.5777004E-489.03
GCAGCCT158.5777004E-489.03
AGCAGCG750.089.02
AGCAGCC550.089.02
CAGCAGC3000.087.516661
CAGCTGC3750.086.626671
AGCAGCA1150.085.130432
CAGCCCT140750.083.277441
Produced by FastQC (version 0.11.5)