FastQCFastQC Report
Tue 7 Mar 2017
1NF_25A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_25A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2203229
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG94350.081.586178
CACCGGC90900.081.2230769
CAGCTGC950.078.0216451
CTTCACC103850.076.699046
CCTTCAC114100.073.346655
GTGTGGC39700.069.873357
CGTAGTG17100.068.346754
CGGGGCA8900.066.977494
GGGCCCG5950.065.475038
TTCACCG120900.065.416637
GCCTTCA32100.063.3136674
TGTGGCA44650.062.757768
CGGGCCC6300.062.5825587
TGCAGCA452.2246604E-762.5825583
ACCTTCA45850.062.0365834
TGTGTGG43050.059.9658666
ATGTGTG42250.057.879615
ACGGGCC6650.057.877116
CAGCGTA8200.057.4628071
GTGGCAC44100.057.2609149
Produced by FastQC (version 0.11.5)