##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1NF_25A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2203229
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.807991361769476	12.0	12.0	12.0	2.0	32.0
2	14.584365946526667	12.0	12.0	12.0	12.0	22.0
3	13.74335032808664	12.0	12.0	12.0	12.0	22.0
4	13.745576605972415	12.0	12.0	12.0	12.0	22.0
5	22.442627616103454	22.0	22.0	22.0	22.0	27.0
6	26.79877171188288	27.0	22.0	32.0	22.0	32.0
7	34.94863493536078	37.0	37.0	37.0	27.0	41.0
8	37.56545915109142	41.0	37.0	41.0	27.0	41.0
9	38.53047822082952	41.0	41.0	41.0	32.0	41.0
10-11	38.89743372114292	41.0	41.0	41.0	37.0	41.0
12-13	39.06851693582465	41.0	41.0	41.0	37.0	41.0
14-15	39.02682131544202	41.0	41.0	41.0	37.0	41.0
16-17	38.92668419851046	41.0	41.0	41.0	37.0	41.0
18-19	38.86876829417187	41.0	41.0	41.0	37.0	41.0
20-21	38.89077236183802	41.0	41.0	41.0	37.0	41.0
22-23	38.870110415213304	41.0	41.0	41.0	37.0	41.0
24-25	38.887011064215294	41.0	41.0	41.0	37.0	41.0
26-27	38.857042095941914	41.0	41.0	41.0	37.0	41.0
28-29	38.81301217440402	41.0	41.0	41.0	37.0	41.0
30-31	38.678150115126485	41.0	41.0	41.0	32.0	41.0
32-33	38.72443400118644	41.0	41.0	41.0	32.0	41.0
34-35	38.69714995581485	41.0	41.0	41.0	32.0	41.0
36-37	38.6265646920951	41.0	41.0	41.0	32.0	41.0
38-39	38.523968457205314	41.0	41.0	41.0	32.0	41.0
40-41	38.57562355070671	41.0	41.0	41.0	32.0	41.0
42-43	38.31452359241822	41.0	41.0	41.0	32.0	41.0
44-45	38.418994575688686	41.0	41.0	41.0	32.0	41.0
46-47	38.437646290966576	41.0	41.0	41.0	32.0	41.0
48-49	38.38764831072939	41.0	41.0	41.0	32.0	41.0
50-51	38.28317959685534	41.0	41.0	41.0	32.0	41.0
52-53	38.250452858055155	41.0	41.0	41.0	32.0	41.0
54-55	38.111179546020864	41.0	37.0	41.0	32.0	41.0
56-57	37.886263298095656	41.0	37.0	41.0	29.5	41.0
58-59	37.88461866651174	41.0	37.0	41.0	29.5	41.0
60-61	37.89513799972677	41.0	37.0	41.0	29.5	41.0
62-63	37.89807051377773	41.0	37.0	41.0	29.5	41.0
64-65	37.88687331185274	41.0	37.0	41.0	29.5	41.0
66-67	37.74306007228481	41.0	37.0	41.0	27.0	41.0
68-69	37.7713285818224	41.0	37.0	41.0	27.0	41.0
70-71	37.75783543154161	41.0	37.0	41.0	27.0	41.0
72-73	37.60490171471054	41.0	37.0	41.0	27.0	41.0
74-75	37.521361147661	41.0	37.0	41.0	27.0	41.0
76-77	36.481503738376716	39.0	34.5	41.0	24.5	41.0
78-79	36.9932707857422	41.0	37.0	41.0	27.0	41.0
80-81	37.648572617735155	41.0	37.0	41.0	27.0	41.0
82-83	37.828116142262104	41.0	37.0	41.0	32.0	41.0
84-85	37.745853245395736	41.0	37.0	41.0	27.0	41.0
86-87	37.61259791878193	41.0	37.0	41.0	27.0	41.0
88-89	37.51756535521274	41.0	37.0	41.0	27.0	41.0
90-91	37.35097645319665	41.0	37.0	41.0	27.0	41.0
92-93	37.246165741282454	41.0	37.0	41.0	27.0	41.0
94-95	37.08565904860548	41.0	37.0	41.0	27.0	41.0
96-97	37.03004930490657	41.0	37.0	41.0	27.0	41.0
98-99	36.910936402888666	41.0	37.0	41.0	27.0	41.0
100	36.76396597902442	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.6927601576273048
1101	2	-0.5545326602494587
1101	3	0.003977129648967903
1101	4	-0.045495602165944504
1101	5	-1.196400045334297
1101	6	-0.7573969840595041
1101	7	-0.4152517380494203
1101	8	-0.15697083849504878
1101	9	-0.2931732866440555
1101	10-11	-0.30786901834331104
1101	12-13	-0.29594931463294216
1101	14-15	-0.25116343791605544
1101	16-17	-0.32026403123732194
1101	18-19	-0.22285167123568073
1101	20-21	-0.12049303538946532
1101	22-23	-0.05523160683393513
1101	24-25	-0.12000124920183453
1101	26-27	-0.1190348626234794
1101	28-29	-0.1010486302704976
1101	30-31	-0.10165392197408352
1101	32-33	-0.23719105420170195
1101	34-35	0.003955576704321118
1101	36-37	-0.12564239186787773
1101	38-39	-0.05605260696518144
1101	40-41	-0.08006573156901453
1101	42-43	-0.005212683678465169
1101	44-45	-0.06369426993504845
1101	46-47	0.004940715583586552
1101	48-49	-0.12737397526436212
1101	50-51	-0.08271042420783914
1101	52-53	0.015687633376408883
1101	54-55	-0.029370083319719242
1101	56-57	-0.3094647501898251
1101	58-59	-0.1669755671307982
1101	60-61	-0.0870269997468398
1101	62-63	-0.3571192505944083
1101	64-65	-0.20041781695618965
1101	66-67	-0.1389392811086978
1101	68-69	-0.12102401520225214
1101	70-71	-0.16069007762580867
1101	72-73	0.03822416905239834
1101	74-75	-0.09875531536201265
1101	76-77	-0.5862520707229137
1101	78-79	-0.4166199624103939
1101	80-81	-0.2124442097558017
1101	82-83	-0.2543796415021049
1101	84-85	-0.29666213159553934
1101	86-87	-0.5373981177474363
1101	88-89	-0.4408690993999542
1101	90-91	-0.5104762314658586
1101	92-93	-0.3353221296311375
1101	94-95	-0.38120865376013313
1101	96-97	-0.4054535066410807
1101	98-99	-0.44247027449374343
1101	100	-0.7783879703849905
1102	1	1.9376447669736852
1102	2	0.8712745510122488
1102	3	-0.10561782560771782
1102	4	0.045155399979641686
1102	5	-0.8705457358466191
1102	6	-0.1449447364274974
1102	7	-0.20883852961360105
1102	8	-0.3416874641688139
1102	9	-0.31757801739919245
1102	10-11	-0.3551674117704948
1102	12-13	-0.43225681371714586
1102	14-15	-0.255820929150552
1102	16-17	-0.4490593632798223
1102	18-19	-0.2598800348339836
1102	20-21	-0.20931636471073745
1102	22-23	-0.2953671450619879
1102	24-25	-0.26145684604101405
1102	26-27	-0.3065485996060602
1102	28-29	-0.43215700852158534
1102	30-31	-0.3463736875295851
1102	32-33	-0.480463857592774
1102	34-35	-0.05371881756015995
1102	36-37	-0.3084894796275748
1102	38-39	-0.2220319884084887
1102	40-41	-0.2181995951934823
1102	42-43	-0.10060883209021654
1102	44-45	-0.21269997405352825
1102	46-47	-0.6299289793245748
1102	48-49	-0.3866654086203738
1102	50-51	-0.15420160637331293
1102	52-53	-0.4298929830870435
1102	54-55	-0.3057123827597792
1102	56-57	-0.26095226915993663
1102	58-59	-0.21851934231526826
1102	60-61	-0.14896640002942974
1102	62-63	-0.4229403296670995
1102	64-65	-0.480374905239195
1102	66-67	-0.3252323890682973
1102	68-69	-0.48777005110156324
1102	70-71	-0.5145691398477936
1102	72-73	-0.24234912102033945
1102	74-75	-0.32750616574665514
1102	76-77	-0.3063839457556199
1102	78-79	-0.17973158520594268
1102	80-81	-0.225601622493258
1102	82-83	-0.3252781709908277
1102	84-85	-0.5120135366926135
1102	86-87	-0.25020621864230463
1102	88-89	-0.4802772316936341
1102	90-91	-0.5945609559412333
1102	92-93	-0.37933542179712987
1102	94-95	-0.36713544218443417
1102	96-97	-0.49549484841720215
1102	98-99	-0.6073794795803451
1102	100	-0.5683141831643113
1103	1	1.456567209819621
1103	2	0.8316921154590506
1103	3	0.26743053849875587
1103	4	1.0806163341129658
1103	5	2.7107876195859184
1103	6	-0.2857934563311453
1103	7	-0.8383326788495182
1103	8	-0.23125498275976497
1103	9	-0.4233608264289259
1103	10-11	-0.38289445346064355
1103	12-13	-0.49613511340443495
1103	14-15	-0.514502048696194
1103	16-17	-0.31095260202856423
1103	18-19	-0.4455533975592587
1103	20-21	-0.34764549919542986
1103	22-23	-0.18402307151584552
1103	24-25	-0.3449401777169072
1103	26-27	-0.49668351524235277
1103	28-29	-0.5064153040100337
1103	30-31	-0.2691005082277016
1103	32-33	-0.5434918904321862
1103	34-35	-0.45024924263832844
1103	36-37	-0.39976222799499084
1103	38-39	-0.2715938717025921
1103	40-41	-0.23985294709014227
1103	42-43	-0.32436464694308853
1103	44-45	-0.7373704196839626
1103	46-47	-0.7407418432708255
1103	48-49	-0.4504293449304697
1103	50-51	-0.30436210183745516
1103	52-53	-0.4861763390720597
1103	54-55	-0.44090674525301665
1103	56-57	-0.5095741075926625
1103	58-59	-0.5088382338192261
1103	60-61	-0.5302726839378948
1103	62-63	-0.4550303065734269
1103	64-65	-0.5599237500033425
1103	66-67	-0.6607952557769536
1103	68-69	-0.7571919359426147
1103	70-71	-0.7753317281064938
1103	72-73	-0.5311660954184134
1103	74-75	-0.5003589519037135
1103	76-77	-0.672976027611071
1103	78-79	-0.8412430602172662
1103	80-81	-0.7164749277606006
1103	82-83	-0.6231986359580617
1103	84-85	-0.6110654808700247
1103	86-87	-0.34997693940781716
1103	88-89	-0.411876330046141
1103	90-91	-0.6839834485103751
1103	92-93	-0.4039202330146878
1103	94-95	-0.4644810516252136
1103	96-97	-0.34757106856946507
1103	98-99	-0.3411498246594107
1103	100	-0.4008873039033105
1104	1	1.4572377933675984
1104	2	-0.6162436656445216
1104	3	0.1609255975044963
1104	4	0.039177637794722386
1104	5	-1.2281724428148273
1104	6	-0.6687505333724957
1104	7	-0.4671453251012707
1104	8	-0.38299204667002584
1104	9	-0.10871797529847527
1104	10-11	-0.14190973378700988
1104	12-13	-0.12258444366263177
1104	14-15	-0.12659331539764906
1104	16-17	-0.07771214030794482
1104	18-19	-0.07896164545631734
1104	20-21	-0.06532786144523328
1104	22-23	0.008262692472420952
1104	24-25	-0.10500005325211248
1104	26-27	-0.1462878990069285
1104	28-29	-0.2474886866130177
1104	30-31	-0.16632025200305378
1104	32-33	-0.2360907804620993
1104	34-35	-0.25273376871253106
1104	36-37	-0.3007453764268391
1104	38-39	-0.2801363765986906
1104	40-41	-0.2745470880417571
1104	42-43	-0.2230854877819084
1104	44-45	-0.1789519306573979
1104	46-47	-0.47505636098377124
1104	48-49	-0.36614400742212183
1104	50-51	-0.029771045570164745
1104	52-53	-0.23999445661217322
1104	54-55	-0.21298690103041196
1104	56-57	-0.23369070495634503
1104	58-59	-0.22194407378825787
1104	60-61	-0.1819614882790077
1104	62-63	-0.3850699243127451
1104	64-65	-0.43875996488188207
1104	66-67	-0.7411996260737439
1104	68-69	-0.5809583708511212
1104	70-71	-0.37328209931236245
1104	72-73	-0.2867100537131648
1104	74-75	-0.3108251056721656
1104	76-77	-0.6789806964438085
1104	78-79	-0.4110561385604967
1104	80-81	-0.3941461790863343
1104	82-83	-0.6641106856990859
1104	84-85	-0.3302032058241551
1104	86-87	-0.4652491289893632
1104	88-89	-0.36359347285517885
1104	90-91	-0.508250436159301
1104	92-93	-0.6071920634552512
1104	94-95	-0.6366515538053079
1104	96-97	-0.39647989726482535
1104	98-99	-0.6863696021042358
1104	100	-0.5280810099576385
1105	1	1.929484340224782
1105	2	0.2649116060697345
1105	3	-0.1024987349496218
1105	4	-0.13152838804200861
1105	5	-0.9554436067904994
1105	6	0.45415066216493827
1105	7	0.058910724148844906
1105	8	0.13737230255706834
1105	9	0.2237562501419461
1105	10-11	0.16013384420070764
1105	12-13	0.009296245987073348
1105	14-15	0.08744730483203966
1105	16-17	0.09436905782216343
1105	18-19	0.016284512500661208
1105	20-21	0.143627822450334
1105	22-23	0.1364100724226276
1105	24-25	0.11078559130368149
1105	26-27	0.14598938948559237
1105	28-29	0.16312775989732842
1105	30-31	0.008238399174622657
1105	32-33	-0.019083539510639014
1105	34-35	0.12134642938847406
1105	36-37	0.09755973298110376
1105	38-39	0.0740383480626079
1105	40-41	0.17316514697037633
1105	42-43	0.2109674873046714
1105	44-45	0.02398704375719518
1105	46-47	-0.14354631704069476
1105	48-49	-0.15976022376703725
1105	50-51	-0.13859325997322713
1105	52-53	-0.028335651726315803
1105	54-55	-0.047090538966969575
1105	56-57	0.019244565646765466
1105	58-59	0.19525206154902008
1105	60-61	-0.10294477086130627
1105	62-63	-0.16522543148359148
1105	64-65	-0.1239220992297021
1105	66-67	-0.1995392867517083
1105	68-69	-0.19288041091047603
1105	70-71	-0.19795252622954962
1105	72-73	-0.004856348975721403
1105	74-75	-0.14767750084769204
1105	76-77	-0.10491465219384111
1105	78-79	-0.2014198793042823
1105	80-81	-0.1727233334479763
1105	82-83	-0.11420129680153224
1105	84-85	0.02826688192973137
1105	86-87	-0.15159502794409718
1105	88-89	-0.0480517507415712
1105	90-91	-0.06553570107440976
1105	92-93	0.0451052256193023
1105	94-95	-0.03898561643676146
1105	96-97	-0.20282386494643134
1105	98-99	-0.4253266881569999
1105	100	-0.4246047183195216
1106	1	1.4439079025247104
1106	2	-0.6632735860324939
1106	3	-0.06707595693964308
1106	4	-0.2716845729761914
1106	5	-1.1314708455170042
1106	6	-0.5824525325899508
1106	7	-0.1694234907507166
1106	8	-0.0663938305543823
1106	9	-0.062026625620411835
1106	10-11	-0.04184252537171318
1106	12-13	-0.11226535673885962
1106	14-15	-0.03062635265906266
1106	16-17	-0.04964191350283187
1106	18-19	-0.00504968981120868
1106	20-21	-0.219746581850238
1106	22-23	-0.021467648733391798
1106	24-25	-0.21900602082704523
1106	26-27	-0.29078253164972523
1106	28-29	-0.00721793651386804
1106	30-31	0.06874484034529615
1106	32-33	0.001220270730748041
1106	34-35	0.00500345037625749
1106	36-37	-0.30971577012175544
1106	38-39	-0.19618987183554282
1106	40-41	-0.20134075421423603
1106	42-43	0.14750371042835297
1106	44-45	-0.2632403332797253
1106	46-47	-0.31545314349733644
1106	48-49	-0.13108650097410646
1106	50-51	-0.33561804461460554
1106	52-53	-0.14307832472929505
1106	54-55	-0.26948721910588347
1106	56-57	-0.16779651362663373
1106	58-59	-0.31972060647283485
1106	60-61	-0.41858075859524746
1106	62-63	-0.37588858935399116
1106	64-65	-0.6759956894152168
1106	66-67	-0.4905868982638353
1106	68-69	-0.363829674055566
1106	70-71	-0.5634620847793741
1106	72-73	-0.4191124950422136
1106	74-75	-0.4956682106211048
1106	76-77	-0.38434845290712616
1106	78-79	-0.21595583166425314
1106	80-81	-0.23593304456569797
1106	82-83	-0.4029837669321097
1106	84-85	-0.5616230734302263
1106	86-87	-0.3949653630822638
1106	88-89	-0.36857381605728534
1106	90-91	-0.37067574165814676
1106	92-93	-0.5027286261640285
1106	94-95	-0.5021643207424944
1106	96-97	-0.3597839694293654
1106	98-99	-0.5925172423568839
1106	100	-0.5450609166377589
1107	1	1.4606302498266217
1107	2	-0.5849636143569903
1107	3	-0.10446066171123114
1107	4	-0.243478094655325
1107	5	-1.2819175010475448
1107	6	-0.48577158262989784
1107	7	-0.4534419379872574
1107	8	-0.449377344244553
1107	9	-0.5726537590360579
1107	10-11	-0.34056610733119186
1107	12-13	-0.3353943058273927
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2223	1	1.2466245255857054
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2223	66-67	0.36421422060399067
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2223	94-95	0.6095874854829901
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2223	100	0.4604176917833058
2224	1	1.1062564821101262
2224	2	-0.971451593463657
2224	3	0.17735935005675252
2224	4	-0.3802796286224783
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2224	6	0.5591077955250086
2224	7	0.49693690854376626
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2224	9	0.2953179668318313
2224	10-11	0.3068808077018943
2224	12-13	0.18185469544231125
2224	14-15	0.1571024317680525
2224	16-17	0.24082247372199106
2224	18-19	0.329008810599845
2224	20-21	0.23064877564041808
2224	22-23	0.22416546969847673
2224	24-25	0.2526503610675377
2224	26-27	0.2215980247180056
2224	28-29	0.12084399789759459
2224	30-31	0.22540381561221778
2224	32-33	0.2454112838534428
2224	34-35	0.22130034612114713
2224	36-37	0.2333200672752085
2224	38-39	0.07538391301458347
2224	40-41	0.23410317532729863
2224	42-43	0.3690493243375741
2224	44-45	0.4973493561301865
2224	46-47	0.4369088568220434
2224	48-49	0.3991169649645201
2224	50-51	0.2611245151583219
2224	52-53	0.20529283943901788
2224	54-55	0.32751287784020633
2224	56-57	0.492317419893034
2224	58-59	0.24158056955787544
2224	60-61	0.22711289328701412
2224	62-63	0.29326601755273884
2224	64-65	0.2855426831092842
2224	66-67	0.30657345546340764
2224	68-69	0.5221543892407254
2224	70-71	0.5317340947730216
2224	72-73	0.40040061955295414
2224	74-75	0.16934342933910074
2224	76-77	0.4728423629804084
2224	78-79	0.35298981397745877
2224	80-81	0.2986698968868353
2224	82-83	0.393846661489917
2224	84-85	0.3509937794012288
2224	86-87	0.516276372391701
2224	88-89	0.4837753524049404
2224	90-91	0.5811756073197643
2224	92-93	0.5352764289190972
2224	94-95	0.7400084392562576
2224	96-97	0.6270327759642171
2224	98-99	0.5974044010260045
2224	100	0.5782668596528282
2225	1	1.1931953273938216
2225	2	1.688923442943631
2225	3	1.14926094586143
2225	4	0.8656140555843539
2225	5	3.189897389427969
2225	6	-0.015995485868099735
2225	7	0.3088777966459375
2225	8	0.44528902577223306
2225	9	0.28232338543917734
2225	10-11	0.14758896639304453
2225	12-13	0.12357421220420406
2225	14-15	0.14366156346743253
2225	16-17	0.2565351316268405
2225	18-19	0.23048630726240305
2225	20-21	0.1157347219015108
2225	22-23	0.20164684245244047
2225	24-25	0.24787693344873674
2225	26-27	0.24555514708608683
2225	28-29	0.25200409821878367
2225	30-31	0.3325433784641021
2225	32-33	0.26737722217386306
2225	34-35	0.30500677581084545
2225	36-37	0.27929187988934956
2225	38-39	0.31332301245998906
2225	40-41	0.32541866520519847
2225	42-43	0.4939001233929119
2225	44-45	0.3761539488874277
2225	46-47	0.3143735862450967
2225	48-49	0.3393458508689662
2225	50-51	0.24311149325579606
2225	52-53	0.2546282842696357
2225	54-55	0.515707467454277
2225	56-57	0.5480264583747214
2225	58-59	0.38886778181498727
2225	60-61	0.3545784999940764
2225	62-63	0.44352922782066884
2225	64-65	0.3949175656158914
2225	66-67	0.5020685519471044
2225	68-69	0.515536785308889
2225	70-71	0.5427492727889884
2225	72-73	0.5433279809383222
2225	74-75	0.45879802437728756
2225	76-77	0.5917406647089578
2225	78-79	0.6409610470828397
2225	80-81	0.5071993031961597
2225	82-83	0.4284097715469528
2225	84-85	0.3786851238164175
2225	86-87	0.2561382277320732
2225	88-89	0.3679628345146213
2225	90-91	0.5441317887380919
2225	92-93	0.5951125873575904
2225	94-95	0.7497213518241281
2225	96-97	0.6647587455259725
2225	98-99	0.5975585523590041
2225	100	0.6892686026908237
2226	1	1.2819974125392495
2226	2	1.645139961677236
2226	3	0.7502616685448427
2226	4	1.0023026148200387
2226	5	3.047970121002276
2226	6	-0.28945610558652035
2226	7	0.04745974665851094
2226	8	0.10866617406547618
2226	9	0.11388596216837499
2226	10-11	0.053618999008229196
2226	12-13	0.0867233225679982
2226	14-15	0.04980367662553675
2226	16-17	0.09723363943967911
2226	18-19	0.09217742620894143
2226	20-21	0.19513073948846227
2226	22-23	0.05343958440476371
2226	24-25	0.09385987732495948
2226	26-27	0.07234995171802439
2226	28-29	0.0754710302338566
2226	30-31	-0.07777976142136112
2226	32-33	-0.026880426888290287
2226	34-35	-0.011334964971460693
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2226	38-39	0.060145988822178253
2226	40-41	0.0911397630875399
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2226	44-45	0.15122892604884441
2226	46-47	0.0872966666856243
2226	48-49	0.06842356965465513
2226	50-51	-0.0489820818202773
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2226	54-55	0.1652052670970079
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2226	60-61	0.3411725735924733
2226	62-63	0.27294810884431797
2226	64-65	0.3413040491497412
2226	66-67	0.1584663876743022
2226	68-69	0.242962869309153
2226	70-71	0.261586905608155
2226	72-73	0.223605185197286
2226	74-75	0.3464910837337456
2226	76-77	0.476358094799032
2226	78-79	0.17583201280459093
2226	80-81	0.0682078988542969
2226	82-83	0.1197098859635446
2226	84-85	0.06112391690529506
2226	86-87	0.3262820089270022
2226	88-89	0.15635327839333257
2226	90-91	0.14253254104456659
2226	92-93	0.10440135554519969
2226	94-95	0.39454780342869356
2226	96-97	0.3196438666972128
2226	98-99	0.547569387639868
2226	100	0.43142420726470476
2227	1	1.3715719062418916
2227	2	1.3975695843939153
2227	3	0.5761768173916924
2227	4	-0.06653243231001582
2227	5	3.26156944550851
2227	6	0.21072191430519993
2227	7	0.4136647501284827
2227	8	0.35733180000047327
2227	9	0.18000187818019242
2227	10-11	0.3222957143681171
2227	12-13	0.27066308621752455
2227	14-15	0.22627035125012895
2227	16-17	0.23204533883461664
2227	18-19	0.2846321732424215
2227	20-21	0.2733383142994512
2227	22-23	0.29269857725140724
2227	24-25	0.23642513104593377
2227	26-27	0.3217169346865063
2227	28-29	0.35024524985304595
2227	30-31	0.21348545617888703
2227	32-33	0.3720648633874859
2227	34-35	0.2986101976674078
2227	36-37	0.45282270932806057
2227	38-39	0.22346825856804742
2227	40-41	0.3650843428339954
2227	42-43	0.4708995040315642
2227	44-45	0.4255370384333972
2227	46-47	0.33643188790829015
2227	48-49	0.18599136836247965
2227	50-51	0.31869617190019284
2227	52-53	0.3525437988884548
2227	54-55	0.4087199430155408
2227	56-57	0.440179972165339
2227	58-59	0.746121585679127
2227	60-61	0.45009801038713704
2227	62-63	0.5451374227933741
2227	64-65	0.4341539538354624
2227	66-67	0.5035070480376689
2227	68-69	0.45851175708531855
2227	70-71	0.6087030867120049
2227	72-73	0.5660697246166819
2227	74-75	0.472496224348788
2227	76-77	0.6950785270902387
2227	78-79	0.4226354059825752
2227	80-81	0.3625388566943286
2227	82-83	0.3285114581194719
2227	84-85	0.2882310497412064
2227	86-87	0.49253346809584997
2227	88-89	0.41022824693625637
2227	90-91	0.43339107749885386
2227	92-93	0.3729264051918264
2227	94-95	0.5415675592443137
2227	96-97	0.49084177262519546
2227	98-99	0.7214264818488232
2227	100	0.8030434441613821
2228	1	1.3959392892157307
2228	2	1.820347821090083
2228	3	-0.23124077651366193
2228	4	0.09164056230209816
2228	5	3.0846741585872124
2228	6	0.29029284007640044
2228	7	0.21806216135538392
2228	8	0.33627651913847245
2228	9	0.10419276824254098
2228	10-11	0.02674306748728128
2228	12-13	0.021026999294960547
2228	14-15	0.21433952511024756
2228	16-17	0.04351864043551501
2228	18-19	0.007126459116754802
2228	20-21	0.0065192183502702505
2228	22-23	0.15576903319011848
2228	24-25	0.21806813251421175
2228	26-27	0.18237668838540344
2228	28-29	0.2501874971435143
2228	30-31	0.15339423530081575
2228	32-33	0.09248494334185153
2228	34-35	0.0736633940668554
2228	36-37	0.11005682792467297
2228	38-39	-0.03489385249861954
2228	40-41	0.04388736270108495
2228	42-43	0.28921042663110086
2228	44-45	0.26664805223803256
2228	46-47	0.14848981734741074
2228	48-49	0.17433983811292109
2228	50-51	0.15743553755137185
2228	52-53	0.16172829433727287
2228	54-55	0.04219458113406205
2228	56-57	0.3215163004342685
2228	58-59	0.30215078709012033
2228	60-61	0.18839776115105877
2228	62-63	0.1565591256619996
2228	64-65	0.002733718453725942
2228	66-67	-0.00895791176817795
2228	68-69	0.22224873609788176
2228	70-71	0.2027133733216573
2228	72-73	0.2906670138685996
2228	74-75	0.3640651177295595
2228	76-77	0.3671405749602883
2228	78-79	0.3124543607058712
2228	80-81	0.1975901753417446
2228	82-83	0.1691617126022109
2228	84-85	0.2088747242021256
2228	86-87	0.03386570949270862
2228	88-89	0.168753675091331
2228	90-91	0.23971318455754442
2228	92-93	0.3055364095075035
2228	94-95	0.4215618470910769
2228	96-97	0.2705124243866166
2228	98-99	0.5225516340216885
2228	100	0.7294387204315669
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	15.0
13	31.0
14	113.0
15	404.0
16	1403.0
17	3576.0
18	5967.0
19	7569.0
20	8950.0
21	10426.0
22	11738.0
23	13442.0
24	15149.0
25	17396.0
26	19557.0
27	22203.0
28	25045.0
29	28366.0
30	32569.0
31	37770.0
32	44418.0
33	53264.0
34	65302.0
35	84166.0
36	118742.0
37	196068.0
38	480683.0
39	897860.0
40	1037.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.499161939507037	19.82073238242188	19.263254440081877	35.416851237989206
2	20.221915582204993	52.10867463426369	11.005042916993858	16.664366866537456
3	40.96750723597048	14.927499592643342	24.080883103844403	20.024110067541777
4	17.400006989740966	16.88539865806051	21.734417983786525	43.980176368411996
5	16.69004901442383	35.025410431689124	26.874963973331866	21.409576580555175
6	17.064998690558266	27.71346056174823	31.3208023314871	23.900738416206398
7	20.23244065868777	26.682428381253153	36.86275915939741	16.222371800661666
8	20.051342824554325	27.408499071136045	35.39836303897598	17.141795065333653
9	20.420482845859418	28.02781735352975	28.148095363668507	23.403604436942324
10-11	19.130444452210824	30.384267817825567	28.87961260495391	21.6056751250097
12-13	24.091140775652462	26.44834014076612	27.463168830838736	21.997350252742677
14-15	21.788089209065422	27.534132856820605	28.485146119627146	22.19263181448683
16-17	20.812180667556575	29.959799911856642	28.12782965365834	21.100189766928448
18-19	21.362146195424987	29.66012611489772	29.95918717482386	19.018540514853427
20-21	22.636003792615295	29.093979790571023	30.037844454661773	18.232171962151913
22-23	22.16737343235769	30.384767085037463	28.73394004890095	18.713919433703897
24-25	22.22000073528444	28.39550496112751	26.41377723332436	22.970717070263692
26-27	19.934241969400365	26.4210846897894	30.127485613161408	23.517187727648828
28-29	19.15943578099806	28.325032991447753	30.949960796061227	21.56557043149296
30-31	19.987031415114554	30.220638433856134	28.38569942297287	21.40663072805645
32-33	20.321310222405387	30.35319978086708	29.013348135849697	20.31214186087783
34-35	21.248404046969245	29.488219336256012	29.965337239115865	19.29803937765888
36-37	21.800525501434485	28.123994373712403	28.828346939877786	21.247133184975326
38-39	21.45415585762498	29.26394425949961	29.055929542787467	20.225970340087947
40-41	20.75111672819461	30.130022269885227	29.76734517232779	19.351515829592373
42-43	20.328849605079096	30.080958558041708	29.578853916938165	20.011337919941035
44-45	20.52351344322356	30.05858673791966	28.9893379217503	20.428561897106473
46-47	19.67055444466458	28.16345779170306	29.136207942450625	23.029779821181727
48-49	20.558439454092152	27.99447992015356	30.606033235764418	20.841047389989875
50-51	21.692842641414035	30.795005875467325	28.311582681600505	19.200568801518138
52-53	20.353504716217877	30.54774303959862	28.2840991641459	20.81465308003761
54-55	22.43958753301772	28.349512403449346	28.33791350032879	20.872986563204147
56-57	21.76072065692536	28.67659408349561	29.588286828356008	19.97439843122302
58-59	21.165271548671765	27.988306429647924	29.188145733844173	21.658276287836145
60-61	19.497681465362003	30.394124790335614	29.890067893702117	20.21812585060027
62-63	20.424656399408523	31.468060550419153	27.318366332532516	20.78891671763981
64-65	19.714146632610568	30.33265970037139	29.032181197810942	20.921012469207106
66-67	20.15544003823479	30.470799903232937	29.4829770305311	19.890783028001174
68-69	20.83455691623522	29.982493876033768	29.536670949774173	19.646278257956844
70-71	19.515924127723448	31.04656846837074	29.396830742514734	20.04067666139108
72-73	19.978517893509935	31.00610513024293	28.672598263730187	20.342778712516946
74-75	19.900405268812275	29.14835452873941	30.85130960059077	20.099930601857547
76-77	19.33636948315404	30.498078048173838	28.886534264027937	21.279018204644185
78-79	19.375516571359583	29.62878575036912	28.53734223723453	22.45835544103677
80-81	20.41539986923723	29.642889643892715	28.641698890241194	21.300011596628853
82-83	19.890783028001174	28.472369417795427	30.30384040878184	21.33300714542156
84-85	20.36499610344635	29.047025978688552	28.562033270259242	22.025944647605854
86-87	21.40149752930812	28.927451481439288	29.337826435654215	20.333224553598377
88-89	20.77410019566736	28.632679580742625	29.64980036119713	20.94341986239288
90-91	20.502521526359722	28.76267060754919	29.111998798127658	21.62280906796343
92-93	20.28175464284466	28.326333758315634	30.214085780461314	21.17782581837839
94-95	20.352196707650453	28.844754675977846	30.168765933999598	20.634282682372103
96-97	20.02195414094495	28.624940938958225	29.913890022326324	21.4392148977705
98-99	20.87620034050024	29.020882531956506	29.513727351991097	20.589189775552157
100	21.184770171416588	30.516936732405032	27.888431025553857	20.409862070624524
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	5.5
2	11.5
3	14.5
4	20.0
5	24.5
6	31.5
7	38.5
8	42.5
9	51.0
10	58.5
11	62.0
12	77.0
13	102.0
14	123.5
15	149.0
16	189.0
17	269.5
18	393.0
19	628.0
20	1037.0
21	1606.0
22	2415.0
23	3682.0
24	5712.0
25	8567.5
26	12583.0
27	17106.5
28	21703.0
29	27067.5
30	33546.0
31	41047.0
32	49225.5
33	58126.5
34	68108.0
35	78404.5
36	88485.0
37	96746.5
38	102800.0
39	106831.5
40	107903.0
41	107281.0
42	105675.5
43	104498.5
44	103775.0
45	100339.5
46	91964.5
47	82898.0
48	77334.5
49	73853.0
50	70342.0
51	65608.5
52	58316.5
53	49035.5
54	40099.5
55	32107.0
56	24825.0
57	18191.0
58	12633.0
59	8987.0
60	7216.5
61	6193.0
62	5099.5
63	3949.0
64	3020.5
65	2373.0
66	2064.5
67	1770.0
68	1432.5
69	1391.5
70	1576.5
71	1456.0
72	983.5
73	533.5
74	294.5
75	196.0
76	133.5
77	109.5
78	89.5
79	62.0
80	40.0
81	35.0
82	27.5
83	24.5
84	25.0
85	18.0
86	10.5
87	14.0
88	13.0
89	8.0
90	10.0
91	12.0
92	13.5
93	9.0
94	9.0
95	11.0
96	13.5
97	14.0
98	32.5
99	77.5
100	79.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.369948380309083
2	0.006308922041240379
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	2.9502153430260766E-4
30-31	0.009667628739454683
32-33	0.0
34-35	0.0
36-37	0.0
38-39	8.85064602907823E-4
40-41	0.0013389438864502964
42-43	1.3616378506274202E-4
44-45	0.0
46-47	0.0019289869550555116
48-49	0.0
50-51	0.0
52-53	0.0015204955998672858
54-55	0.019721054869920467
56-57	0.010847714876665112
58-59	0.014024869861462426
60-61	2.4963360594836036E-4
62-63	0.012322822548178151
64-65	6.808189253137101E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.1346982088561834E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2203229.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.58197927850703
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.80121881572468	65.60607921879257
2	8.261750819459841	12.488789584812045
3	2.249541213231863	5.100743320939148
4	0.9519355306236743	2.8779668620029257
5	0.5103190676159494	1.9285462596987861
6	0.3113377507139538	1.4118914053847427
7	0.19878595533960974	1.0517245170135625
8	0.14693781578842718	0.888468075851999
9	0.10030554233777206	0.6823162280243608
>10	0.4391343895076511	5.636164348306671
>50	0.01954392123151464	1.0137807930928746
>100	0.008883485901544823	1.14689723687795
>500	2.445540188264219E-4	0.12033003786002237
>1k	6.1138504706589E-5	0.046302111342440194
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	2.2693964177123668E-5	0.0	0.0	0.0	0.0
12-13	4.5387928354247335E-5	0.0	0.0	0.0	0.0
14-15	4.5387928354247335E-5	0.0	0.0	0.0	0.0
16-17	4.5387928354247335E-5	0.0	0.0	0.0	0.0
18-19	4.5387928354247335E-5	0.0	0.0	0.0	0.0
20-21	4.5387928354247335E-5	0.0	0.0	0.0	0.0
22-23	4.5387928354247335E-5	0.0	0.0	0.0	0.0
24-25	4.5387928354247335E-5	0.0	0.0	0.0	0.0
26-27	4.5387928354247335E-5	0.0	0.0	0.0	0.0
28-29	4.5387928354247335E-5	0.0	0.0	0.0	0.0
30-31	4.5387928354247335E-5	0.0	0.0	0.0	0.0
32-33	6.8081892531371E-5	0.0	0.0	0.0	0.0
34-35	9.077585670849467E-5	0.0	0.0	0.0	0.0
36-37	9.077585670849467E-5	0.0	0.0	0.0	0.0
38-39	9.077585670849467E-5	0.0	0.0	0.0	0.0
40-41	9.077585670849467E-5	0.0	0.0	0.0	0.0
42-43	9.077585670849467E-5	0.0	0.0	0.0	0.0
44-45	9.077585670849467E-5	0.0	0.0	0.0	0.0
46-47	9.077585670849467E-5	0.0	0.0	0.0	0.0
48-49	9.077585670849467E-5	0.0	0.0	0.0	0.0
50-51	1.5885774923986567E-4	0.0	0.0	0.0	0.0
52-53	2.72327570125484E-4	0.0	0.0	0.0	0.0
54-55	3.631034268339787E-4	0.0	0.0	0.0	0.0
56-57	3.631034268339787E-4	0.0	0.0	0.0	0.0
58-59	4.0849135518822603E-4	0.0	0.0	0.0	4.5387928354247335E-5
60-61	5.673491044280917E-4	0.0	0.0	0.0	4.5387928354247335E-5
62-63	7.262068536679574E-4	0.0	0.0	0.0	4.5387928354247335E-5
64-65	7.715947820222047E-4	0.0	0.0	0.0	4.5387928354247335E-5
66-67	9.077585670849466E-4	0.0	0.0	0.0	4.5387928354247335E-5
68-69	9.53146495439194E-4	0.0	0.0	2.2693964177123668E-5	4.5387928354247335E-5
70-71	0.0010439223521476887	0.0	0.0	4.5387928354247335E-5	4.5387928354247335E-5
72-73	0.0010439223521476887	0.0	0.0	4.5387928354247335E-5	4.5387928354247335E-5
74-75	0.001089310280501936	0.0	0.0	4.5387928354247335E-5	4.5387928354247335E-5
76-77	0.0011346982088561834	0.0	0.0	4.5387928354247335E-5	4.5387928354247335E-5
78-79	0.0012935559580960492	0.0	0.0	4.5387928354247335E-5	4.5387928354247335E-5
80-81	0.0014751076715130383	0.0	0.0	4.5387928354247335E-5	4.5387928354247335E-5
82-83	0.0016112714565757803	0.0	0.0	4.5387928354247335E-5	4.5387928354247335E-5
84-85	0.001702047313284275	0.0	0.0	4.5387928354247335E-5	4.5387928354247335E-5
86-87	0.001770129205815646	4.5387928354247335E-5	0.0	4.5387928354247335E-5	4.5387928354247335E-5
88	0.001770129205815646	4.5387928354247335E-5	0.0	4.5387928354247335E-5	4.5387928354247335E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	9435	0.0	81.58617	8
CACCGGC	9090	0.0	81.223076	9
CAGCTGC	95	0.0	78.021645	1
CTTCACC	10385	0.0	76.69904	6
CCTTCAC	11410	0.0	73.34665	5
GTGTGGC	3970	0.0	69.87335	7
CGTAGTG	1710	0.0	68.34675	4
CGGGGCA	890	0.0	66.9774	94
GGGCCCG	595	0.0	65.47503	8
TTCACCG	12090	0.0	65.41663	7
GCCTTCA	3210	0.0	63.313667	4
TGTGGCA	4465	0.0	62.75776	8
CGGGCCC	630	0.0	62.582558	7
TGCAGCA	45	2.2246604E-7	62.582558	3
ACCTTCA	4585	0.0	62.036583	4
TGTGTGG	4305	0.0	59.965866	6
ATGTGTG	4225	0.0	57.87961	5
ACGGGCC	665	0.0	57.87711	6
CAGCGTA	820	0.0	57.462807	1
GTGGCAC	4410	0.0	57.260914	9
>>END_MODULE