FastQCFastQC Report
Tue 7 Mar 2017
1NF_26A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_26A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2934794
Sequences flagged as poor quality0
Sequence length95
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA154780.5273964714388812No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG70300.2395398109714004No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA64770.22069692114676534No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT64440.21957248106681423No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG58220.1983784892568269No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG55280.1883607503627171No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA54830.1868274229809656No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG53570.18253410631206143No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC48250.16440676926557707No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA45600.1553771746841516No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT44150.1504364531207301No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT42340.14426906965190744No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA42320.14420092176827404No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC39980.13622761938316624No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA36070.12290470813283658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGCG1050.088.999772
GCTGCTA355.5479177E-1088.999763
GCTGCCT355.5479177E-1088.999763
GCTGCCG202.411375E-588.999763
GCTGCCA401.6370905E-1188.999763
AGCTGCC950.088.999762
GCAGCTC202.411375E-588.999763
GCAGCAC355.5479177E-1088.999763
AGCAGCG1550.088.999762
GCTGCAG301.9390427E-888.9997563
AGCAGCA1200.088.9997562
CAGCAGC4000.087.887271
CAGCTGC3850.086.688081
AGCAGCC950.084.315562
CAGCCCT164800.083.329271
AGCTGCT750.083.0664442
CTGCTGC2150.082.7904741
GCTGCCC650.082.1536253
TGCTGCC650.082.1536252
AGCTGCA1150.081.260642
Produced by FastQC (version 0.11.5)