##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1NF_26A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2934794
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.777997024663401	12.0	12.0	12.0	2.0	27.0
2	14.690900962725152	12.0	12.0	12.0	12.0	22.0
3	13.71827562684127	12.0	12.0	12.0	12.0	22.0
4	13.791166603175554	12.0	12.0	12.0	12.0	22.0
5	22.44696493178056	22.0	22.0	22.0	22.0	27.0
6	26.88341975620776	27.0	22.0	32.0	22.0	32.0
7	35.01018333825134	37.0	37.0	37.0	27.0	41.0
8	37.61815445990417	41.0	37.0	41.0	32.0	41.0
9	38.563135947531585	41.0	41.0	41.0	32.0	41.0
10-11	38.91630911743721	41.0	41.0	41.0	37.0	41.0
12-13	39.09095050623655	41.0	41.0	41.0	37.0	41.0
14-15	39.04788223636821	41.0	41.0	41.0	37.0	41.0
16-17	38.95133934443099	41.0	41.0	41.0	37.0	41.0
18-19	38.89423721051631	41.0	41.0	41.0	37.0	41.0
20-21	38.9090823751173	41.0	41.0	41.0	37.0	41.0
22-23	38.88741697032228	41.0	41.0	41.0	37.0	41.0
24-25	38.91147368435399	41.0	41.0	41.0	37.0	41.0
26-27	38.87443905773284	41.0	41.0	41.0	37.0	41.0
28-29	38.82722790764871	41.0	41.0	41.0	37.0	41.0
30-31	38.71280778139794	41.0	41.0	41.0	32.0	41.0
32-33	38.74750646893786	41.0	41.0	41.0	32.0	41.0
34-35	38.727724330907044	41.0	41.0	41.0	32.0	41.0
36-37	38.646217247275274	41.0	41.0	41.0	32.0	41.0
38-39	38.562380868980924	41.0	41.0	41.0	32.0	41.0
40-41	38.6034110060195	41.0	41.0	41.0	32.0	41.0
42-43	38.343601799649306	41.0	41.0	41.0	32.0	41.0
44-45	38.45694927821169	41.0	41.0	41.0	32.0	41.0
46-47	38.466341589903756	41.0	41.0	41.0	32.0	41.0
48-49	38.42151135650407	41.0	41.0	41.0	32.0	41.0
50-51	38.319200768435536	41.0	41.0	41.0	32.0	41.0
52-53	38.280914946670876	41.0	41.0	41.0	32.0	41.0
54-55	38.145894737415986	41.0	39.0	41.0	32.0	41.0
56-57	37.93525848833002	41.0	37.0	41.0	29.5	41.0
58-59	37.934023989417994	41.0	37.0	41.0	29.5	41.0
60-61	37.935129859199655	41.0	37.0	41.0	29.5	41.0
62-63	37.94171430771632	41.0	37.0	41.0	32.0	41.0
64-65	37.92377812548342	41.0	37.0	41.0	32.0	41.0
66-67	37.77955471491355	41.0	37.0	41.0	27.0	41.0
68-69	37.804584921462975	41.0	37.0	41.0	27.0	41.0
70-71	37.78322958953848	41.0	37.0	41.0	27.0	41.0
72-73	37.629874703301155	41.0	37.0	41.0	27.0	41.0
74-75	37.55106661660069	41.0	37.0	41.0	27.0	41.0
76-77	36.50878886218249	39.0	34.5	41.0	24.5	41.0
78-79	37.01839635081713	41.0	37.0	41.0	27.0	41.0
80-81	37.661189678048956	41.0	37.0	41.0	27.0	41.0
82-83	37.84648377364817	41.0	37.0	41.0	32.0	41.0
84-85	37.765608420897685	41.0	37.0	41.0	27.0	41.0
86-87	37.628149880366394	41.0	37.0	41.0	27.0	41.0
88-89	37.54856814481698	41.0	37.0	41.0	27.0	41.0
90-91	37.38794664974782	41.0	37.0	41.0	27.0	41.0
92-93	37.2812810711757	41.0	37.0	41.0	27.0	41.0
94-95	37.12601208125681	41.0	37.0	41.0	27.0	41.0
96-97	37.069360064113525	41.0	37.0	41.0	27.0	41.0
98-99	36.943337931043885	41.0	37.0	41.0	27.0	41.0
100	36.79858756696382	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.4409417934381938
1101	2	-0.5579227163437022
1101	3	0.05915342930192935
1101	4	-0.08739551947869018
1101	5	-1.0873475310097582
1101	6	-0.9001790181903289
1101	7	-0.45550116097712845
1101	8	-0.14227029291362214
1101	9	-0.33972432707419387
1101	10-11	-0.262088879669399
1101	12-13	-0.17945744596640623
1101	14-15	-0.21720643715539723
1101	16-17	-0.32084166064137776
1101	18-19	-0.04477691882523516
1101	20-21	-0.06693511381854478
1101	22-23	-0.12840150560531782
1101	24-25	-0.10780844308986559
1101	26-27	-0.019412049125456576
1101	28-29	0.007747481479690066
1101	30-31	-0.1351410012120624
1101	32-33	-0.34109370410537565
1101	34-35	-0.14309093400641615
1101	36-37	-0.1519297547148284
1101	38-39	-0.025326024760076393
1101	40-41	-0.07509182848317408
1101	42-43	-0.009625823533298217
1101	44-45	-0.10339324663180349
1101	46-47	0.05490544427136257
1101	48-49	-0.08891989161764968
1101	50-51	-0.04875046022111462
1101	52-53	-0.14823517687756294
1101	54-55	-0.10311950923476587
1101	56-57	0.0033565938792037286
1101	58-59	-0.08856353120263094
1101	60-61	0.04695191299823165
1101	62-63	-0.29764121167469426
1101	64-65	0.014186806413327702
1101	66-67	-0.08681906155929653
1101	68-69	-0.09231155819427528
1101	70-71	-0.07448021134302962
1101	72-73	-0.05180179579586763
1101	74-75	0.04349873809246674
1101	76-77	-0.46554801559548054
1101	78-79	-0.22832340261285822
1101	80-81	-0.2454445889332817
1101	82-83	-0.14266804548498158
1101	84-85	-0.30713205241761443
1101	86-87	-0.27805922063447497
1101	88-89	-0.40936601777023185
1101	90-91	-0.20626472698321408
1101	92-93	0.16444879716507188
1101	94-95	0.030181721806997075
1101	96-97	-0.25853785159792153
1101	98-99	-0.5415887696748598
1101	100	-0.5752717084786738
1102	1	1.8204870087318739
1102	2	0.760010721165397
1102	3	0.012655847916789753
1102	4	0.022514641988770734
1102	5	-0.7709849426028335
1102	6	0.03603226400031545
1102	7	0.015044149134915585
1102	8	-0.3098043360656817
1102	9	-0.19928414588899557
1102	10-11	-0.32300355583482343
1102	12-13	-0.3095563034424629
1102	14-15	-0.17964410528067276
1102	16-17	-0.31268593753191
1102	18-19	-0.27251584430173637
1102	20-21	-0.10682281005335881
1102	22-23	-0.2087141523591285
1102	24-25	-0.1280533581751726
1102	26-27	-0.1605847208306912
1102	28-29	-0.2709550449443938
1102	30-31	-0.23557470189811625
1102	32-33	-0.24779795965802975
1102	34-35	-0.06796059608720384
1102	36-37	-0.29435835910051367
1102	38-39	-0.10964904031253297
1102	40-41	-0.17967103280032148
1102	42-43	-0.16322970526269387
1102	44-45	-0.06980701455676552
1102	46-47	-0.42778243980833963
1102	48-49	-0.24003236486132806
1102	50-51	-0.20707868555732745
1102	52-53	-0.3727325088978688
1102	54-55	-0.32412745083262706
1102	56-57	-0.21491587527309974
1102	58-59	-0.10840640149633884
1102	60-61	-0.13054492701183307
1102	62-63	-0.251597563870412
1102	64-65	-0.29911098372153333
1102	66-67	-0.019694454551547835
1102	68-69	-0.2163629452674698
1102	70-71	-0.46281292375296346
1102	72-73	-0.22038332054980714
1102	74-75	-0.3637697240507478
1102	76-77	-0.3261504631038079
1102	78-79	-0.34933440527598236
1102	80-81	-0.38906081198190634
1102	82-83	-0.3719292909801766
1102	84-85	-0.3167084831109932
1102	86-87	-0.2037475310235095
1102	88-89	-0.259190603798487
1102	90-91	-0.39528371529367945
1102	92-93	-0.3023282669448406
1102	94-95	-0.2346563441074423
1102	96-97	-0.09906232406811455
1102	98-99	-0.3518889556532372
1102	100	-0.36221977414673745
1103	1	1.6302228411933601
1103	2	0.9174525082929517
1103	3	0.5193357442185693
1103	4	0.8037021634588868
1103	5	2.8816612322145865
1103	6	-0.018426986402086953
1103	7	-0.4704051563483702
1103	8	-0.20181577010126261
1103	9	-0.3790320055286216
1103	10-11	-0.3517147854005529
1103	12-13	-0.5658374717924417
1103	14-15	-0.4970353109642005
1103	16-17	-0.4283350412841216
1103	18-19	-0.34289705968055273
1103	20-21	-0.2850623817387117
1103	22-23	-0.2459973090669223
1103	24-25	-0.3850493493766294
1103	26-27	-0.17948171043661176
1103	28-29	-0.09613332027678467
1103	30-31	-0.12314875668477043
1103	32-33	-0.4420018301088149
1103	34-35	-0.42673930148707484
1103	36-37	-0.3486428750281121
1103	38-39	-0.24899669305651884
1103	40-41	-0.4142834047523465
1103	42-43	-0.24679308348341067
1103	44-45	-0.7090584968262661
1103	46-47	-0.738738440055613
1103	48-49	-0.5162180826459775
1103	50-51	-0.3749393580933429
1103	52-53	-0.11749606959990899
1103	54-55	-0.24999222215582506
1103	56-57	-0.30861917410035034
1103	58-59	-0.5972187000489342
1103	60-61	-0.35905512976798803
1103	62-63	-0.3389747391502951
1103	64-65	-0.5150544677219457
1103	66-67	-0.5984813919562271
1103	68-69	-0.5652980665838996
1103	70-71	-0.6308775183854181
1103	72-73	-0.4046072154305449
1103	74-75	-0.4884224656917766
1103	76-77	-0.6183227811878496
1103	78-79	-0.5336755984002224
1103	80-81	-0.25172483585159
1103	82-83	-0.25543851951142926
1103	84-85	-0.5434938774060214
1103	86-87	-0.2905622381417601
1103	88-89	-0.2743410922020786
1103	90-91	-0.5817959040601224
1103	92-93	-0.47177125287765875
1103	94-95	-0.36567691707688965
1103	96-97	-0.2741763944580171
1103	98-99	-0.3995524982792915
1103	100	-0.320427795030362
1104	1	1.4239432030918096
1104	2	-0.6129405808219097
1104	3	0.10086898070700201
1104	4	0.009490091711064963
1104	5	-1.0844100138323753
1104	6	-0.42594464923935504
1104	7	-0.1432781047180569
1104	8	-0.02462243443456913
1104	9	-0.14852121578839927
1104	10-11	-0.06492939080920479
1104	12-13	0.08712231176230745
1104	14-15	-0.06405784134180692
1104	16-17	-0.10119583241817054
1104	18-19	-0.12152332302641611
1104	20-21	0.10044270881333262
1104	22-23	0.09229387918284004
1104	24-25	-0.15656177143868888
1104	26-27	-0.009567229233091723
1104	28-29	-0.08725969977457027
1104	30-31	-0.07606269894331774
1104	32-33	-0.023487366870128312
1104	34-35	-0.1291015994988669
1104	36-37	-0.12647760166840527
1104	38-39	-0.20199743952627358
1104	40-41	-0.15707160867177805
1104	42-43	-0.13286375651416904
1104	44-45	-0.128216596642595
1104	46-47	-0.2391756359311401
1104	48-49	-0.3962033236070326
1104	50-51	-0.05666749752521838
1104	52-53	-0.05819222233814969
1104	54-55	-0.044870154217782954
1104	56-57	-0.14869050687408958
1104	58-59	-0.1889919683784811
1104	60-61	-0.2570607236450826
1104	62-63	-0.2356400945906998
1104	64-65	-0.18893445335007897
1104	66-67	-0.6717483276823941
1104	68-69	-0.546657694649916
1104	70-71	-0.2451757438936184
1104	72-73	-0.24205243043551405
1104	74-75	-0.2519059241078381
1104	76-77	-0.2604674259642721
1104	78-79	-0.25989395474196897
1104	80-81	-0.4020908693095393
1104	82-83	-0.3683515945022151
1104	84-85	-0.27525903058719337
1104	86-87	-0.2087369182593335
1104	88-89	-0.23672319799016606
1104	90-91	-0.16744664913606044
1104	92-93	-0.2759329766342873
1104	94-95	-0.13379630887439475
1104	96-97	-0.24551296249362764
1104	98-99	-0.37209085164754896
1104	100	-0.24535942025918445
1105	1	1.4988208161637395
1105	2	0.4705942719591185
1105	3	-0.016404459675527505
1105	4	-0.16750751389814056
1105	5	-1.2104388955380259
1105	6	0.29089107123265734
1105	7	-0.1896896180600649
1105	8	-0.1379652769823636
1105	9	-0.09955720556231995
1105	10-11	-0.1502639856135488
1105	12-13	-0.1827785657041332
1105	14-15	-0.25337691820490704
1105	16-17	-0.18323821438698218
1105	18-19	-0.18471397542488432
1105	20-21	-0.18495480059107194
1105	22-23	-0.2229150473587751
1105	24-25	-0.16146171904462392
1105	26-27	-0.16124655134093757
1105	28-29	-0.19702503815140915
1105	30-31	-0.15864898969996943
1105	32-33	-0.28134260189534643
1105	34-35	-0.14586911529904256
1105	36-37	-0.38795489668035543
1105	38-39	-0.2520398793133367
1105	40-41	-0.15080279911376238
1105	42-43	-0.025482973869657144
1105	44-45	-0.28968314759328706
1105	46-47	-0.3630117456332229
1105	48-49	-0.2734050365769818
1105	50-51	-0.2386029753394041
1105	52-53	-0.1538744955609701
1105	54-55	-0.2762710814556044
1105	56-57	-0.20089636249178966
1105	58-59	-0.25153782808518343
1105	60-61	-0.3161947850909783
1105	62-63	-0.5541709626810416
1105	64-65	-0.4797843130663111
1105	66-67	-0.363975975415471
1105	68-69	-0.2879544089639907
1105	70-71	-0.4600299226926836
1105	72-73	-0.4625888168903103
1105	74-75	-0.4826780033772522
1105	76-77	-0.41575629070592157
1105	78-79	-0.41253018377614126
1105	80-81	-0.48993206403723377
1105	82-83	-0.4510269968904055
1105	84-85	-0.3382668402232909
1105	86-87	-0.3069199052693108
1105	88-89	-0.37481299628792186
1105	90-91	-0.3603170033354175
1105	92-93	-0.22409124187223028
1105	94-95	-0.22324635599328957
1105	96-97	-0.2843040156858194
1105	98-99	-0.4144439858437039
1105	100	-0.5707454762856656
1106	1	1.4603532802278973
1106	2	-0.6274746814855217
1106	3	-0.1524586579086904
1106	4	-0.2961854506539243
1106	5	-1.1226937209139791
1106	6	-0.8238168521133709
1106	7	-0.17030596493688677
1106	8	-0.246745754004003
1106	9	-0.19714879681840358
1106	10-11	-0.1586059496034764
1106	12-13	-0.12181511258913957
1106	14-15	-0.17564372841878395
1106	16-17	-0.11568728255986116
1106	18-19	-0.12709108677425007
1106	20-21	-0.2136382786914055
1106	22-23	-0.17462939896321217
1106	24-25	-0.3653353279953677
1106	26-27	-0.22832579675021947
1106	28-29	-0.1868546545021701
1106	30-31	-0.19446877860312384
1106	32-33	-0.17323858712400408
1106	34-35	-0.1172604677545479
1106	36-37	-0.30714655939141267
1106	38-39	-0.10338703842580088
1106	40-41	-0.12736828869528694
1106	42-43	-0.053942781795598194
1106	44-45	-0.16887526436599387
1106	46-47	-0.22297562084632006
1106	48-49	-0.2191696868613846
1106	50-51	-0.2838129534637943
1106	52-53	-0.31523135426684235
1106	54-55	-0.30082401742723874
1106	56-57	-0.2080848754488258
1106	58-59	-0.1795350756351084
1106	60-61	-0.17824525284915893
1106	62-63	-0.30220064875416597
1106	64-65	-0.48718835594765864
1106	66-67	-0.5325014071997245
1106	68-69	-0.3714373785152745
1106	70-71	-0.5051911339977408
1106	72-73	-0.38565389438101505
1106	74-75	-0.5694231968209564
1106	76-77	-0.4036135020563947
1106	78-79	-0.32649237059682434
1106	80-81	-0.4257056402178705
1106	82-83	-0.3790592699200701
1106	84-85	-0.6141997352875492
1106	86-87	-0.4149700002189718
1106	88-89	-0.5266611467185456
1106	90-91	-0.4517861556710159
1106	92-93	-0.5193143638866644
1106	94-95	-0.3886635565060246
1106	96-97	-0.41847878700453833
1106	98-99	-0.5448693131639075
1106	100	-0.5190731104481898
1107	1	1.654140531532878
1107	2	-0.5592688686043168
1107	3	-0.07268404604796963
1107	4	-0.2526559467246319
1107	5	-1.1799539621097743
1107	6	-0.28536729587246157
1107	7	-0.33843553305631247
1107	8	-0.3784267733700517
1107	9	-0.1332061319076061
1107	10-11	-0.13446397645219577
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2224	10-11	0.07409813075433647
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2224	16-17	0.2320802865252034
2224	18-19	0.0689049123850154
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2224	24-25	0.09966885363280653
2224	26-27	0.11321754005678741
2224	28-29	0.13473562824645313
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2224	36-37	0.07690005143201972
2224	38-39	0.02853598140055169
2224	40-41	0.10076414765797637
2224	42-43	0.31932625196167663
2224	44-45	0.3887405167606772
2224	46-47	0.24681825207628094
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2224	50-51	0.17906998887383452
2224	52-53	0.17847614047489202
2224	54-55	0.15079873356966544
2224	56-57	0.4067645344933055
2224	58-59	0.3492982427550828
2224	60-61	0.2661478378370461
2224	62-63	0.3541378617631068
2224	64-65	0.3782258310159534
2224	66-67	0.28377534162478923
2224	68-69	0.22787195467304144
2224	70-71	0.351940489546962
2224	72-73	0.3498268899719079
2224	74-75	0.2917051519723941
2224	76-77	0.5587197288797228
2224	78-79	0.29073755428085235
2224	80-81	0.3036173475664796
2224	82-83	0.2269627155963576
2224	84-85	0.15192694526398753
2224	86-87	0.25648821950978373
2224	88-89	0.2753343497634475
2224	90-91	0.2764013253132305
2224	92-93	0.22437093662468754
2224	94-95	0.5168779459698882
2224	96-97	0.35091933780346096
2224	98-99	0.34251113562989133
2224	100	0.5449157953762551
2225	1	1.4083035308746084
2225	2	1.906347278966372
2225	3	0.8904099681381066
2225	4	1.0053242756077143
2225	5	3.1230496694664502
2225	6	-0.0296569935862685
2225	7	0.19714503838071096
2225	8	0.24710017499647563
2225	9	0.25930882707999103
2225	10-11	0.21451200780850144
2225	12-13	0.19935744634572217
2225	14-15	0.24771040688659696
2225	16-17	0.14577582195178707
2225	18-19	0.033842078974110734
2225	20-21	0.16725659583909191
2225	22-23	0.09003891432325162
2225	24-25	0.18194804163179867
2225	26-27	0.2350080237487404
2225	28-29	0.1950395915846741
2225	30-31	0.2907246505069736
2225	32-33	0.14769340942686426
2225	34-35	0.18526470652895455
2225	36-37	0.2710198466416429
2225	38-39	0.10618196614868225
2225	40-41	0.20581982056621229
2225	42-43	0.3491497455709265
2225	44-45	0.23862630674313579
2225	46-47	0.31300277823000755
2225	48-49	0.28227134904759765
2225	50-51	0.21385785898232257
2225	52-53	0.2483393748739431
2225	54-55	0.2704203010037034
2225	56-57	0.4407489962025153
2225	58-59	0.4639704707454868
2225	60-61	0.42016595390879985
2225	62-63	0.3876479401706092
2225	64-65	0.4965169770544051
2225	66-67	0.5257860107737642
2225	68-69	0.5689417337715312
2225	70-71	0.4393716592150696
2225	72-73	0.5098530258066418
2225	74-75	0.4406371629995718
2225	76-77	0.5220103378804737
2225	78-79	0.4252218190762349
2225	80-81	0.4621447901929514
2225	82-83	0.3458073684909877
2225	84-85	0.3790795713783339
2225	86-87	0.3528113013901333
2225	88-89	0.39500174441455016
2225	90-91	0.5305537008099961
2225	92-93	0.45654916153608127
2225	94-95	0.5945790665885582
2225	96-97	0.627635531280248
2225	98-99	0.5845171504521289
2225	100	0.8098092828990815
2226	1	1.640613599966402
2226	2	1.4875365733145784
2226	3	0.8454101827088927
2226	4	1.0805485020800258
2226	5	3.1089452164073847
2226	6	-0.33286268114259343
2226	7	-0.007360948144246038
2226	8	0.1433154324098993
2226	9	0.15877096964082682
2226	10-11	0.13797961477204268
2226	12-13	0.16595864508119007
2226	14-15	0.03789679737681695
2226	16-17	0.11284550023864881
2226	18-19	0.09245180332217728
2226	20-21	0.11793421038412077
2226	22-23	0.16815913118279724
2226	24-25	0.18382653734754228
2226	26-27	0.09200789500626883
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2226	30-31	0.14438365461242597
2226	32-33	0.16029343517536176
2226	34-35	0.1880774434508652
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2226	38-39	0.2185482527866469
2226	40-41	0.22069572712779006
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2226	60-61	0.4696844570629892
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2226	66-67	0.465490904418175
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2226	74-75	0.43614562424092895
2226	76-77	0.6623376298540933
2226	78-79	0.5077870843572541
2226	80-81	0.49926539327991293
2226	82-83	0.3591794199307614
2226	84-85	0.36052849637869855
2226	86-87	0.2867578303500409
2226	88-89	0.20066555490563331
2226	90-91	0.36756916421136765
2226	92-93	0.2525947649806497
2226	94-95	0.5555745319925904
2226	96-97	0.520085214430722
2226	98-99	0.47430157833488806
2226	100	0.6620475423008543
2227	1	1.1884235337662457
2227	2	1.9040195120322316
2227	3	0.6353532197827239
2227	4	0.0020567269920839237
2227	5	3.2677399029660528
2227	6	0.46988757375270396
2227	7	0.44479919872192397
2227	8	0.5222162018886891
2227	9	0.5104980123615661
2227	10-11	0.3857221574506653
2227	12-13	0.3345349205017314
2227	14-15	0.3089003150771532
2227	16-17	0.3307436525019227
2227	18-19	0.2788771400599188
2227	20-21	0.20191502134331074
2227	22-23	0.24735836780406117
2227	24-25	0.27780432732543403
2227	26-27	0.29716084670833
2227	28-29	0.4131484616779275
2227	30-31	0.30254132078818685
2227	32-33	0.25451188698053784
2227	34-35	0.24116508967072292
2227	36-37	0.2547305384655445
2227	38-39	0.3915871566352038
2227	40-41	0.2519790051248805
2227	42-43	0.5090246823163938
2227	44-45	0.3574523025502643
2227	46-47	0.27734890705239224
2227	48-49	0.17281208496894607
2227	50-51	0.4021301065063554
2227	52-53	0.34109582683664286
2227	54-55	0.4818350338888351
2227	56-57	0.4760117736188363
2227	58-59	0.44407384673045414
2227	60-61	0.4101146273708096
2227	62-63	0.5158225949815716
2227	64-65	0.4897382594949491
2227	66-67	0.39608168054893156
2227	68-69	0.3501995954067496
2227	70-71	0.580489276507052
2227	72-73	0.5481223816947391
2227	74-75	0.442490701639052
2227	76-77	0.593508928207946
2227	78-79	0.6532641815428022
2227	80-81	0.3595574989329222
2227	82-83	0.29488204498922244
2227	84-85	0.5272433825545093
2227	86-87	0.4563246390609237
2227	88-89	0.3912867151367365
2227	90-91	0.5542405366373586
2227	92-93	0.4584243874451275
2227	94-95	0.5174475694440446
2227	96-97	0.4098606451384157
2227	98-99	0.6354128346854822
2227	100	0.6266733037188601
2228	1	1.2675442588305952
2228	2	1.546257458820186
2228	3	-0.19129789575092104
2228	4	-0.0594764759776627
2228	5	3.0896411301470046
2228	6	0.16994870878307466
2228	7	0.3411626038419442
2228	8	0.278842415375685
2228	9	0.35519147727251266
2228	10-11	0.22634378364247
2228	12-13	0.22923944174795707
2228	14-15	0.19177202834663376
2228	16-17	0.14479726633375378
2228	18-19	0.03839657247246464
2228	20-21	0.11819059724830794
2228	22-23	0.23735498586621162
2228	24-25	0.253023249990342
2228	26-27	0.07502859270456241
2228	28-29	0.1765535094369639
2228	30-31	0.17605243710227825
2228	32-33	0.12604312437956366
2228	34-35	0.19755818068965425
2228	36-37	0.2044790610356415
2228	38-39	0.16477865091697907
2228	40-41	0.26473649850192515
2228	42-43	0.37321827079814796
2228	44-45	0.2349660290266442
2228	46-47	0.19219041123429292
2228	48-49	0.2806888205606626
2228	50-51	0.21875327046819137
2228	52-53	0.27152784771794103
2228	54-55	0.1489869508261208
2228	56-57	0.37158136277120946
2228	58-59	0.4459058742599993
2228	60-61	0.3470448105880095
2228	62-63	0.26606612341225144
2228	64-65	0.19835772066841884
2228	66-67	0.32631909547657756
2228	68-69	0.3820869335181527
2228	70-71	0.3838424534329903
2228	72-73	0.34967872347858275
2228	74-75	0.3001351096626266
2228	76-77	0.2747393899291808
2228	78-79	0.41147520868357645
2228	80-81	0.35383898889332954
2228	82-83	0.27934579827540773
2228	84-85	0.24851367598819962
2228	86-87	0.24587835333920083
2228	88-89	0.3429666277266463
2228	90-91	0.33235658046380223
2228	92-93	0.3114162462246597
2228	94-95	0.6944594250150615
2228	96-97	0.530996455958018
2228	98-99	0.6161954064324249
2228	100	0.5787265302176436
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	20.0
13	44.0
14	146.0
15	502.0
16	1905.0
17	4607.0
18	7674.0
19	9934.0
20	11654.0
21	13382.0
22	15719.0
23	17924.0
24	20557.0
25	22944.0
26	25914.0
27	29383.0
28	32998.0
29	37839.0
30	43207.0
31	49474.0
32	58584.0
33	70001.0
34	85757.0
35	110733.0
36	156203.0
37	255709.0
38	631127.0
39	1219283.0
40	1570.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.713702370337387	19.511613680415294	19.125612463083034	35.64907148616429
2	18.229706908231456	50.9721792489992	14.354620185617062	16.44349365715228
3	41.1647972566388	15.328673835369704	24.359188413224235	19.147340494767263
4	18.07026319394138	15.96868468451278	21.656886309567213	44.30416581197862
5	16.86189899529575	35.14641913538054	26.72415849289592	21.267523376427782
6	17.628630834055134	28.715303356896598	29.887378807507442	23.768687001540822
7	20.064304342996476	26.48492534740087	36.117015368029236	17.333754941573414
8	21.09688107580975	28.044523738293048	33.67929742257889	17.17929776331831
9	20.719205504713447	28.43218297434164	27.65601265369903	23.192598867245877
10-11	19.595719495133217	30.430261885502013	28.665316884251503	21.308701735113264
12-13	24.08848798246146	26.613418181991648	27.461876370198386	21.836217465348504
14-15	21.812195336367733	27.505951013938283	28.550845476718294	22.131008172975683
16-17	20.72630992158223	29.76198670162199	28.366914338791755	21.14478903800403
18-19	21.358143024689298	29.50684102529854	29.84022047203313	19.294795477979036
20-21	22.735224346240315	28.77694311764301	29.999618371851653	18.48821416426502
22-23	21.72891862256772	30.273777307708823	28.788851960307948	19.208452109415518
24-25	22.266077278337082	28.522393735301353	26.468314300765233	22.74321468559633
26-27	20.121923378608518	26.542595493925635	29.936734912228935	23.398746215236912
28-29	19.427484076335457	28.219735496627862	30.74624256716766	21.606537859869025
30-31	20.179384040167086	29.992107797400752	28.36643673868017	21.462071423751986
32-33	20.60795408468192	30.06849884523411	28.814918525797722	20.508628544286243
34-35	21.247061974366854	29.31694694755407	29.925882361760316	19.51010871631876
36-37	21.688438779689477	28.105243502610406	28.97797937436154	21.228338343338578
38-39	21.515188274082686	29.14053736518507	29.111335698543396	20.23293866218885
40-41	20.869513364874972	29.869000543488443	29.75069384222479	19.510792249411786
42-43	20.355946982255297	29.890152314083014	29.508915626359766	20.244985077301926
44-45	20.459834659604727	30.09742762183649	28.940106188032278	20.502631530526504
46-47	19.84797943391472	28.34261497931074	28.95093235861711	22.858473228157436
48-49	20.834804901946253	27.959054018621753	30.315028979040605	20.89111210039139
50-51	21.720553469851716	30.605759722828928	28.26939471731236	19.404292090007
52-53	20.507504033556465	30.303245074972185	28.18393698962946	21.00531390184189
54-55	22.184881338464496	28.56500926308385	28.296846734473256	20.953262663978396
56-57	21.55423663459443	28.57294989775835	29.602318238441395	20.270495229205824
58-59	21.20804984885277	28.1753399896844	29.197221705836355	21.419388455626475
60-61	19.619959114614524	30.3124295626654	29.622550441863037	20.445060880857042
62-63	20.54897523997114	31.18453348009477	27.36746956645456	20.89902171347953
64-65	19.69822398005992	30.269516499036985	28.885858199514956	21.14640132138814
66-67	20.191928292070926	30.190585778763346	29.49813513316437	20.119350796001353
68-69	20.83321691403213	29.920669048662358	29.351072000283494	19.89504203702202
70-71	19.4495933956523	31.064718682128966	29.185762271559774	20.299925650658956
72-73	20.043774793051913	30.846015086578475	28.549789184521977	20.560420935847628
74-75	20.017009711754895	29.29135741724973	30.527577063330508	20.164055807664866
76-77	19.44797488341601	30.684385343570963	28.68777161190871	21.179868161104324
78-79	19.571901809803347	29.399184406128676	28.647087325379566	22.381826458688412
80-81	20.57968071288284	29.667321796327368	28.508038740788088	21.244958750001704
82-83	20.177191312235205	28.61589263164638	29.949103753108396	21.257812303010024
84-85	20.49256268071967	29.093285593469254	28.261881413141776	22.152270312669305
86-87	21.40387025460731	28.71087374446043	29.47256263983094	20.412693361101326
88-89	20.755783881253677	28.08956948937472	29.64853751234329	21.506109117028316
90-91	20.44330879782363	29.303555888420107	29.022531053286876	21.230604260469388
92-93	20.226325936334884	29.85192487104717	29.986039906037696	19.93570928658025
94-95	20.33011516310855	29.534781657588233	29.84603007911288	20.289073100190336
96-97	20.145775137880207	29.37674671544238	29.73769198110668	20.739786165570735
98-99	21.410923560563365	29.4765663279944	29.13667875837282	19.975831353069413
100	21.053027912691658	31.38894927548577	27.609024687933804	19.948998123888764
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	3.5
2	9.0
3	19.0
4	26.5
5	34.5
6	44.5
7	58.0
8	70.5
9	76.0
10	79.0
11	89.5
12	102.0
13	121.0
14	150.0
15	183.5
16	253.0
17	366.0
18	514.0
19	801.5
20	1262.0
21	1967.5
22	3183.5
23	4935.0
24	7349.0
25	11055.0
26	16073.0
27	21736.5
28	27864.5
29	35090.5
30	43717.0
31	53363.5
32	64473.5
33	76564.5
34	89355.0
35	103809.5
36	118008.5
37	128770.5
38	136110.5
39	141318.0
40	144200.0
41	144248.5
42	142049.5
43	139417.0
44	137842.5
45	133589.5
46	123458.0
47	111452.5
48	103004.5
49	98915.0
50	95533.5
51	89355.5
52	78477.0
53	64709.0
54	52730.0
55	43439.0
56	34264.0
57	25056.0
58	17871.0
59	13174.0
60	10345.5
61	8566.5
62	6904.0
63	5261.5
64	3797.5
65	2904.5
66	2486.0
67	2223.5
68	1938.0
69	1786.5
70	1856.5
71	1659.0
72	1103.5
73	572.0
74	307.0
75	201.5
76	138.0
77	98.0
78	80.5
79	76.5
80	61.0
81	43.0
82	33.5
83	31.0
84	26.0
85	20.0
86	17.5
87	19.0
88	17.0
89	13.5
90	16.0
91	20.0
92	16.5
93	15.5
94	13.5
95	10.5
96	18.5
97	27.0
98	48.5
99	97.0
100	95.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.381548415323188
2	0.005111091272504986
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	3.7481335998369904E-4
30-31	0.008586633337808377
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0010222182545009972
40-41	0.0016696231490182956
42-43	1.0222182545009973E-4
44-45	0.0
46-47	0.0020273995380936448
48-49	1.7036970908349958E-5
50-51	0.0
52-53	0.0013288837308512965
54-55	0.01928585106825215
56-57	0.01105699411951912
58-59	0.013748835523038416
60-61	1.533327381751496E-4
62-63	0.011125142003152521
64-65	5.111091272504987E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	2.0444365090019947E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2934794.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.99997703973806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.6959652604849	63.414994616740714
2	8.828806626078741	13.06662975236228
3	2.499822038240522	5.5496032029968925
4	1.0833921251149956	3.2068396953417073
5	0.5698359476809062	2.1083923522402217
6	0.32886123784869536	1.4601434430037972
7	0.2225767003643763	1.1529469501581153
8	0.1469320209951404	0.869837294403416
9	0.11244198077101032	0.7488633595821688
>10	0.4802363002575959	5.9402076633244
>50	0.022042580964011377	1.1119576613553346
>100	0.008619565428243296	1.1320305944785947
>500	4.2085419390714015E-4	0.19737868518749502
>1k	4.676157703058773E-5	0.04017472882488909
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.407394181669991E-5	0.0
2	0.0	0.0	0.0	3.407394181669991E-5	0.0
3	0.0	0.0	0.0	3.407394181669991E-5	0.0
4	0.0	0.0	0.0	3.407394181669991E-5	0.0
5	0.0	0.0	0.0	3.407394181669991E-5	0.0
6	0.0	0.0	0.0	3.407394181669991E-5	0.0
7	0.0	0.0	0.0	3.407394181669991E-5	0.0
8	0.0	0.0	0.0	3.407394181669991E-5	0.0
9	0.0	0.0	0.0	3.407394181669991E-5	0.0
10-11	0.0	0.0	0.0	3.407394181669991E-5	0.0
12-13	0.0	0.0	0.0	3.407394181669991E-5	0.0
14-15	0.0	0.0	0.0	8.518485454174978E-5	0.0
16-17	0.0	0.0	0.0	1.0222182545009973E-4	0.0
18-19	0.0	0.0	0.0	1.0222182545009973E-4	0.0
20-21	0.0	0.0	0.0	1.0222182545009973E-4	0.0
22-23	0.0	0.0	0.0	1.0222182545009973E-4	0.0
24-25	0.0	0.0	0.0	1.0222182545009973E-4	0.0
26-27	0.0	0.0	0.0	1.0222182545009973E-4	0.0
28-29	0.0	0.0	0.0	1.0222182545009973E-4	0.0
30-31	0.0	0.0	0.0	1.0222182545009973E-4	0.0
32-33	0.0	0.0	0.0	1.0222182545009973E-4	0.0
34-35	0.0	0.0	0.0	1.0222182545009973E-4	0.0
36-37	0.0	3.407394181669991E-5	0.0	1.0222182545009973E-4	0.0
38-39	3.407394181669991E-5	3.407394181669991E-5	0.0	1.0222182545009973E-4	0.0
40-41	1.0222182545009973E-4	3.407394181669991E-5	0.0	1.0222182545009973E-4	0.0
42-43	1.0222182545009973E-4	3.407394181669991E-5	0.0	1.0222182545009973E-4	0.0
44-45	1.0222182545009973E-4	3.407394181669991E-5	0.0	1.0222182545009973E-4	0.0
46-47	1.1925879635844969E-4	3.407394181669991E-5	0.0	1.0222182545009973E-4	0.0
48-49	1.3629576726679964E-4	3.407394181669991E-5	0.0	1.0222182545009973E-4	0.0
50-51	2.0444365090019947E-4	3.407394181669991E-5	0.0	1.0222182545009973E-4	0.0
52-53	2.3851759271689937E-4	3.407394181669991E-5	0.0	1.0222182545009973E-4	0.0
54-55	2.725915345335993E-4	3.407394181669991E-5	0.0	1.0222182545009973E-4	0.0
56-57	3.91850330892049E-4	3.407394181669991E-5	1.0222182545009973E-4	1.0222182545009973E-4	0.0
58-59	4.7703518543379874E-4	3.407394181669991E-5	2.0444365090019947E-4	1.0222182545009973E-4	0.0
60-61	5.792570108838985E-4	3.407394181669991E-5	2.0444365090019947E-4	1.0222182545009973E-4	0.0
62-63	6.303679236089484E-4	3.407394181669991E-5	2.0444365090019947E-4	1.0222182545009973E-4	0.0
64-65	7.325897490590481E-4	3.407394181669991E-5	2.0444365090019947E-4	1.0222182545009973E-4	0.0
66-67	8.007376326924478E-4	3.407394181669991E-5	2.0444365090019947E-4	1.0222182545009973E-4	0.0
68-69	8.688855163258478E-4	3.407394181669991E-5	2.0444365090019947E-4	1.0222182545009973E-4	0.0
70-71	9.029594581425476E-4	5.111091272504987E-5	2.0444365090019947E-4	1.0222182545009973E-4	0.0
72-73	9.370333999592476E-4	6.814788363339982E-5	2.0444365090019947E-4	1.0222182545009973E-4	0.0
74-75	0.0010222182545009972	6.814788363339982E-5	2.0444365090019947E-4	1.0222182545009973E-4	0.0
76-77	0.0012948097890345966	6.814788363339982E-5	2.0444365090019947E-4	1.0222182545009973E-4	0.0
78-79	0.0016355492072015958	6.814788363339982E-5	2.0444365090019947E-4	1.0222182545009973E-4	0.0
80-81	0.0020103625671852947	6.814788363339982E-5	2.0444365090019947E-4	1.0222182545009973E-4	0.0
82-83	0.0021977692471771442	6.814788363339982E-5	2.0444365090019947E-4	1.3629576726679964E-4	0.0
84-85	0.002317028043535594	6.814788363339982E-5	2.0444365090019947E-4	1.3629576726679964E-4	0.0
86-87	0.002351101985352294	1.0222182545009973E-4	2.0444365090019947E-4	1.3629576726679964E-4	0.0
88	0.002351101985352294	1.0222182545009973E-4	2.0444365090019947E-4	1.3629576726679964E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	11465	0.0	81.5533	8
CACCGGC	11090	0.0	81.09428	9
CTTCACC	13080	0.0	73.70873	6
CCTTCAC	14390	0.0	70.03215	5
GTGTGGC	4880	0.0	67.714096	7
TTCACCG	14270	0.0	67.16732	7
CGTAGTG	2225	0.0	64.764145	4
CGGGCCC	825	0.0	64.291084	7
CGGGGCA	1235	0.0	64.23119	94
AACGGGC	870	0.0	61.5052	5
TGTGGCA	5380	0.0	60.984737	8
CAGCAGC	80	2.3646862E-11	59.582386	1
GGGCCCG	885	0.0	59.401993	8
ACCTTCA	5915	0.0	58.960384	4
GGCCCGA	895	0.0	58.73828	9
TGTGTGG	5320	0.0	57.34928	6
GCGTAGT	1435	0.0	56.91459	3
ACGGGCC	980	0.0	55.080513	6
GCCTTCA	4370	0.0	54.993935	4
ATGTGTG	5330	0.0	54.335567	5
>>END_MODULE