FastQCFastQC Report
Tue 7 Mar 2017
1NF_26A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_26A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2934794
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG114650.081.55338
CACCGGC110900.081.094289
CTTCACC130800.073.708736
CCTTCAC143900.070.032155
GTGTGGC48800.067.7140967
TTCACCG142700.067.167327
CGTAGTG22250.064.7641454
CGGGCCC8250.064.2910847
CGGGGCA12350.064.2311994
AACGGGC8700.061.50525
TGTGGCA53800.060.9847378
CAGCAGC802.3646862E-1159.5823861
GGGCCCG8850.059.4019938
ACCTTCA59150.058.9603844
GGCCCGA8950.058.738289
TGTGTGG53200.057.349286
GCGTAGT14350.056.914593
ACGGGCC9800.055.0805136
GCCTTCA43700.054.9939354
ATGTGTG53300.054.3355675
Produced by FastQC (version 0.11.5)