FastQCFastQC Report
Tue 7 Mar 2017
1NF_27A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_27A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2272652
Sequences flagged as poor quality0
Sequence length95
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA138540.609596189825807No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG64170.2823573516754875No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC58750.25850856180356696No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA56930.25050029656982237No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG55320.24341606193997145No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT52320.23021562474149146No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA49100.21604715548178954No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA48550.21362707532873487No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG47070.20711485964415144No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG46430.20429876637514235No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA41890.18432210474810926No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA41480.1825180449976503No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG41120.18093399253383272No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG39530.1739377608186383No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT37250.1639054285477935No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA35350.15554515165542282No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT34320.15101300155061134No Hit
CAGCAGGCGCGCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACGAAA33280.14643684998847162No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA33020.14529281209793668No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA32800.1443247800367148No Hit
CAGCAGAAGAGCGGCCGGGAGAGTTGAGGGTGGACGTCTCGAGCTCGAGG32210.14172869405434707No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC32140.1414206838530492No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC29840.13130034866754786No Hit
CAGCTCATCAACTTCAGATTCAGCCGCACCACATTGACGGGTACGAGGTA27690.12184003534197053No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA27630.12157602659800093No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA27100.1192439493596028No Hit
CAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTTGGCACTCTCATGCGA24990.10995964186333851No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC22960.10102734602570036No Hit
CAGCGGTTCTGACGTGCAAATCGATCGTCAAACTTGAGCATAGGGGCGAA22900.10076333728173079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCAGC3900.088.9996951
GCTGCTA355.5479177E-1088.9996953
GCTGCAT700.088.9996953
GCTGCCT450.088.999693
GCTGCCG158.577855E-488.999693
GCAGCAA750.088.999693
AGCTGCC3300.087.6512152
GCTGCCC2500.087.2196963
CAGCTGC6600.086.9769741
AGCAGCA1900.086.657592
CAGCCCT135250.082.978641
AGCTGCA1450.082.8617862
GCAGCAT650.082.1535643
GCTGCTT600.081.5830463
TGCTGCA600.081.5830462
CAGCGTA46550.080.969641
AGCCCTT116000.080.866962
GCCCTTC105000.080.311633
GCTGCAA501.8189894E-1280.0997243
CTGCTGC1450.079.792831
Produced by FastQC (version 0.11.5)