##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1NF_27A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2272652
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.771830883038847	12.0	12.0	12.0	2.0	27.0
2	14.67766688432721	12.0	12.0	12.0	12.0	22.0
3	13.758012225364904	12.0	12.0	12.0	12.0	22.0
4	13.782446234619291	12.0	12.0	12.0	12.0	22.0
5	22.46393596555918	22.0	22.0	22.0	22.0	27.0
6	26.873330804716254	27.0	22.0	32.0	22.0	32.0
7	35.01157194326276	37.0	37.0	37.0	27.0	41.0
8	37.64117735579402	41.0	37.0	41.0	32.0	41.0
9	38.592966719057735	41.0	41.0	41.0	32.0	41.0
10-11	38.95222475768397	41.0	41.0	41.0	37.0	41.0
12-13	39.12351583084432	41.0	41.0	41.0	37.0	41.0
14-15	39.083948180363734	41.0	41.0	41.0	37.0	41.0
16-17	38.9887712681044	41.0	41.0	41.0	37.0	41.0
18-19	38.91830557428062	41.0	41.0	41.0	37.0	41.0
20-21	38.93146068997805	41.0	41.0	41.0	37.0	41.0
22-23	38.91690170778456	41.0	41.0	41.0	37.0	41.0
24-25	38.92976201371789	41.0	41.0	41.0	37.0	41.0
26-27	38.901291310768215	41.0	41.0	41.0	37.0	41.0
28-29	38.85227500734824	41.0	41.0	41.0	37.0	41.0
30-31	38.7314522856997	41.0	41.0	41.0	32.0	41.0
32-33	38.7737634270447	41.0	41.0	41.0	32.0	41.0
34-35	38.74492949206478	41.0	41.0	41.0	32.0	41.0
36-37	38.67285576498294	41.0	41.0	41.0	32.0	41.0
38-39	38.58460116199049	41.0	41.0	41.0	32.0	41.0
40-41	38.62076947988517	41.0	41.0	41.0	32.0	41.0
42-43	38.37142928173781	41.0	41.0	41.0	32.0	41.0
44-45	38.480855405931045	41.0	41.0	41.0	32.0	41.0
46-47	38.48372694103629	41.0	41.0	41.0	32.0	41.0
48-49	38.44202059972226	41.0	41.0	41.0	32.0	41.0
50-51	38.34077742654837	41.0	41.0	41.0	32.0	41.0
52-53	38.31351126349304	41.0	41.0	41.0	32.0	41.0
54-55	38.17609295219857	41.0	39.0	41.0	32.0	41.0
56-57	37.957093958951916	41.0	37.0	41.0	29.5	41.0
58-59	37.95579525593888	41.0	37.0	41.0	29.5	41.0
60-61	37.956697725828676	41.0	37.0	41.0	32.0	41.0
62-63	37.95472470048208	41.0	37.0	41.0	32.0	41.0
64-65	37.9492335386148	41.0	37.0	41.0	32.0	41.0
66-67	37.81921341234822	41.0	37.0	41.0	27.0	41.0
68-69	37.840611761061524	41.0	37.0	41.0	29.5	41.0
70-71	37.81464694110669	41.0	37.0	41.0	29.5	41.0
72-73	37.665452959802025	41.0	37.0	41.0	27.0	41.0
74-75	37.57756642900013	41.0	37.0	41.0	27.0	41.0
76-77	36.52659932096951	39.0	34.5	41.0	24.5	41.0
78-79	37.03573468353272	41.0	37.0	41.0	27.0	41.0
80-81	37.700997117024514	41.0	37.0	41.0	27.0	41.0
82-83	37.88091533591593	41.0	37.0	41.0	32.0	41.0
84-85	37.80133232012644	41.0	37.0	41.0	27.0	41.0
86-87	37.662693628412974	41.0	37.0	41.0	27.0	41.0
88-89	37.57261230491954	41.0	37.0	41.0	27.0	41.0
90-91	37.40853703074646	41.0	37.0	41.0	27.0	41.0
92-93	37.30945938929497	41.0	37.0	41.0	27.0	41.0
94-95	37.14755756710662	41.0	37.0	41.0	27.0	41.0
96-97	37.074048072472166	41.0	37.0	41.0	27.0	41.0
98-99	36.967933277950166	41.0	37.0	41.0	27.0	41.0
100	36.8161240700292	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.087673501454292
1101	2	-0.6982136218502184
1101	3	0.08252384921084044
1101	4	0.22379388593115124
1101	5	-1.0949846431409505
1101	6	-0.7116111637163023
1101	7	-0.22769022654429705
1101	8	-0.17616521838171195
1101	9	-0.10194882579499165
1101	10-11	-0.02221802804103845
1101	12-13	-0.06453531430561554
1101	14-15	-0.1747291406243363
1101	16-17	-0.12905568951894963
1101	18-19	-0.07901970840942596
1101	20-21	-0.07990734917203213
1101	22-23	-0.11598306397566205
1101	24-25	-0.21040096846387968
1101	26-27	-0.05168153700876843
1101	28-29	-0.1285096650813955
1101	30-31	-0.2105664933894218
1101	32-33	-0.4664453350690678
1101	34-35	-0.26229093445049045
1101	36-37	-0.2595936425314136
1101	38-39	-0.0744378311502345
1101	40-41	-0.0540488347082686
1101	42-43	0.06638599601382111
1101	44-45	-0.0623522425335139
1101	46-47	-0.17679073997539518
1101	48-49	-0.22806305807141314
1101	50-51	-0.0847730376909297
1101	52-53	-0.1522518903922716
1101	54-55	-0.08648908030852454
1101	56-57	0.10998714762978778
1101	58-59	-0.019428975909264068
1101	60-61	-0.13710017013131193
1101	62-63	-0.27630260619020675
1101	64-65	-0.1387111255212261
1101	66-67	-0.19221415725522917
1101	68-69	-0.11141901095958673
1101	70-71	-0.2997184683002274
1101	72-73	-0.2054485618459978
1101	74-75	-0.10641164720863117
1101	76-77	-0.48105201493692107
1101	78-79	-0.09148785748545407
1101	80-81	-0.19389466069436878
1101	82-83	-0.17805233292484246
1101	84-85	-0.18180731496711644
1101	86-87	0.0227446314518005
1101	88-89	-0.27568361464830815
1101	90-91	-0.1893486084320628
1101	92-93	0.043033817375999206
1101	94-95	-0.025416377111703525
1101	96-97	-0.22576333006655602
1101	98-99	-0.332564778432598
1101	100	-0.512476108193141
1102	1	1.6151426116008896
1102	2	0.7359924252975034
1102	3	-0.21912474765851186
1102	4	-0.057550928551604486
1102	5	-0.8376285731338449
1102	6	-0.09618167451958826
1102	7	0.007328219294805649
1102	8	-0.21285848505669236
1102	9	-0.38857529557409975
1102	10-11	-0.19771474955042834
1102	12-13	-0.31140748780732963
1102	14-15	-0.3350625956764617
1102	16-17	-0.3477989479803796
1102	18-19	-0.17755094362751578
1102	20-21	-0.1719477815805064
1102	22-23	-0.394393515562264
1102	24-25	-0.3416026891519195
1102	26-27	-0.2435292727424354
1102	28-29	-0.3174512410527228
1102	30-31	-0.3395289099922678
1102	32-33	-0.3927171479665077
1102	34-35	-0.24841969313656165
1102	36-37	-0.4699756614007953
1102	38-39	-0.3177202899885003
1102	40-41	-0.19036818720389448
1102	42-43	-0.09092539429181556
1102	44-45	-0.14448051038609577
1102	46-47	-0.5111924525488476
1102	48-49	-0.40213242887433864
1102	50-51	-0.3060078203684711
1102	52-53	-0.33709151846715457
1102	54-55	-0.3823426753669281
1102	56-57	-0.12741236015131108
1102	58-59	-0.25166644431681107
1102	60-61	-0.14304892850071127
1102	62-63	-0.24877214185777063
1102	64-65	-0.28388279849153975
1102	66-67	-0.3473038085853304
1102	68-69	-0.5967167667818032
1102	70-71	-0.46303292037766397
1102	72-73	-0.3866061965827612
1102	74-75	-0.39109803825645884
1102	76-77	-0.22550465643254114
1102	78-79	-0.3612952399773519
1102	80-81	-0.40603677777002645
1102	82-83	-0.3107820944549715
1102	84-85	-0.378526399879334
1102	86-87	-0.21634694066014504
1102	88-89	-0.4773989836739432
1102	90-91	-0.6016733531689553
1102	92-93	-0.42947047741701283
1102	94-95	-0.17601034995357878
1102	96-97	-0.32503229989018934
1102	98-99	-0.29540046994375047
1102	100	-0.5620760195264509
1103	1	1.2830646099618246
1103	2	0.49928142814558285
1103	3	0.508205239926788
1103	4	1.167574029863209
1103	5	2.929127214307023
1103	6	-0.19039545077911413
1103	7	-0.44796161931409983
1103	8	-0.10450777384856735
1103	9	0.02689520531440337
1103	10-11	-0.22532122791395892
1103	12-13	-0.43126106737906866
1103	14-15	-0.4315549358879167
1103	16-17	-0.43008284700749044
1103	18-19	-0.21513837394697077
1103	20-21	-0.25302773873762163
1103	22-23	-0.35288338500506455
1103	24-25	-0.37294676295852014
1103	26-27	-0.30099746514594017
1103	28-29	-0.1731470930824841
1103	30-31	-0.1306652810051645
1103	32-33	-0.5320498375746752
1103	34-35	-0.5361891854321499
1103	36-37	-0.1979243554230834
1103	38-39	-0.25855674052038324
1103	40-41	-0.3266286443569584
1103	42-43	-0.2519625085638495
1103	44-45	-0.6148231723248969
1103	46-47	-0.39501409064006765
1103	48-49	-0.3584127049188339
1103	50-51	-0.28647698241680786
1103	52-53	-0.2893436098977631
1103	54-55	-0.2801374433418786
1103	56-57	-0.31187765579155524
1103	58-59	-0.5053056552936752
1103	60-61	-0.3999627193657673
1103	62-63	-0.25331101285037505
1103	64-65	-0.33084261705841556
1103	66-67	-0.48845458227265226
1103	68-69	-0.2636508633892589
1103	70-71	-0.5020438826385956
1103	72-73	-0.3437338975913491
1103	74-75	-0.3253386724609868
1103	76-77	-0.5931379927410703
1103	78-79	-0.6475864295976876
1103	80-81	-0.3361133147991069
1103	82-83	-0.43284323887931464
1103	84-85	-0.4436285168696159
1103	86-87	-0.4533956906702912
1103	88-89	-0.3203555687389752
1103	90-91	-0.6255457860443911
1103	92-93	-0.5413493367251974
1103	94-95	-0.4424471485839021
1103	96-97	-0.48373405093083477
1103	98-99	-0.34471644433538273
1103	100	-0.3313390894463595
1104	1	1.6310164601905548
1104	2	-0.5685224159863864
1104	3	-0.07295331755267576
1104	4	0.22135715354835384
1104	5	-1.0807350289879132
1104	6	-0.4447290725460533
1104	7	-0.18234121082296895
1104	8	-0.08398357033713921
1104	9	0.06275816240626142
1104	10-11	-0.018121589489723533
1104	12-13	0.004142344814255239
1104	14-15	0.021277272012049764
1104	16-17	0.022979794264813336
1104	18-19	0.07689569816278663
1104	20-21	0.06331063557464489
1104	22-23	0.04972019435095376
1104	24-25	-0.06007950579945032
1104	26-27	-0.04816706207437704
1104	28-29	0.02511521305098796
1104	30-31	0.016136918953563395
1104	32-33	0.013630750504503908
1104	34-35	-0.05175328836125459
1104	36-37	-0.04311545370437386
1104	38-39	0.06409319496335542
1104	40-41	0.07927192434193131
1104	42-43	0.12990943911543695
1104	44-45	0.01337061133768458
1104	46-47	-0.16453775829540263
1104	48-49	-0.26728552094937186
1104	50-51	-0.05006454404789196
1104	52-53	0.10495432710711583
1104	54-55	0.007296222859430657
1104	56-57	-0.02550782154089859
1104	58-59	-0.007280035881649383
1104	60-61	-0.024464230846000135
1104	62-63	-0.055852109555630136
1104	64-65	-0.2273329121656502
1104	66-67	-0.5006714911252672
1104	68-69	-0.39372344799504333
1104	70-71	-0.3086405050166334
1104	72-73	-0.14754994032968227
1104	74-75	-0.1632266978567003
1104	76-77	-0.21181677192463866
1104	78-79	0.03255285739849967
1104	80-81	-0.21559348354801955
1104	82-83	-0.189306812358204
1104	84-85	-0.24921812126139997
1104	86-87	-0.14360172581309882
1104	88-89	-0.09674648135172959
1104	90-91	-0.1281385110098867
1104	92-93	-0.19439865785551547
1104	94-95	-0.11667219085755676
1104	96-97	-0.0920459974713026
1104	98-99	-0.28101616989979306
1104	100	-0.1985436288694089
1105	1	1.3862795518477338
1105	2	0.5649706874812708
1105	3	-0.04265368623606669
1105	4	-0.1336988576379987
1105	5	-1.2641603315626853
1105	6	0.42412330234241935
1105	7	-0.04682984253521738
1105	8	-0.09095695753973843
1105	9	-0.09300330063656759
1105	10-11	-0.16324372517996721
1105	12-13	-0.285134459509365
1105	14-15	-0.1677798178304286
1105	16-17	-0.1344796846519074
1105	18-19	-0.1724586110488744
1105	20-21	-0.08460478937897165
1105	22-23	-0.18045241262208833
1105	24-25	-0.09502244137161853
1105	26-27	-0.17582176210949285
1105	28-29	-0.11242150683247587
1105	30-31	-0.1541596430560901
1105	32-33	-0.22845081632782183
1105	34-35	-0.03694620383386393
1105	36-37	-0.22010144314899094
1105	38-39	-0.054091740066802174
1105	40-41	0.004872143689858888
1105	42-43	0.05649252092933921
1105	44-45	-0.10029577112483423
1105	46-47	-0.29873306347761996
1105	48-49	-0.334187493003526
1105	50-51	-0.19958622334979026
1105	52-53	-0.1799328306535486
1105	54-55	-0.1614063033805735
1105	56-57	-0.1650058676679862
1105	58-59	-0.13592578483043383
1105	60-61	-0.1624694110345999
1105	62-63	-0.45480825194508157
1105	64-65	-0.33277460278684146
1105	66-67	-0.41173441216221107
1105	68-69	-0.34961413472559144
1105	70-71	-0.3429820421480443
1105	72-73	-0.3177406894482999
1105	74-75	-0.3313741340690015
1105	76-77	-0.27220941839301105
1105	78-79	-0.31266292147846286
1105	80-81	-0.49620096195350527
1105	82-83	-0.2563057999267784
1105	84-85	-0.24760685729587095
1105	86-87	-0.43585778616701276
1105	88-89	-0.25915558429056773
1105	90-91	-0.38969685875503757
1105	92-93	-0.23242788420688498
1105	94-95	-0.37325965425375074
1105	96-97	-0.23678019328966826
1105	98-99	-0.30381078365707737
1105	100	-0.3379387945643302
1106	1	1.3491942702461497
1106	2	-0.7288539840132469
1106	3	-0.07653882586771665
1106	4	-0.18337378867130383
1106	5	-1.2267950375387322
1106	6	-0.6278760954490465
1106	7	-0.13789351881467127
1106	8	0.08000917259403195
1106	9	0.06499279955405513
1106	10-11	-0.07572129691224916
1106	12-13	0.01281148572893187
1106	14-15	-0.04543766301483032
1106	16-17	-0.1521444683259432
1106	18-19	0.021535449778546933
1106	20-21	-0.042026900468989936
1106	22-23	-0.03660821298851147
1106	24-25	-0.08125601246095471
1106	26-27	-0.1463440907364415
1106	28-29	-0.1318907341945703
1106	30-31	-0.09395042650176322
1106	32-33	-0.21872638491667828
1106	34-35	-0.10841482898741361
1106	36-37	-0.4950881880154867
1106	38-39	-0.16383188126578574
1106	40-41	-0.1795193093127878
1106	42-43	0.07973791581594014
1106	44-45	-0.06953586104882703
1106	46-47	-0.2586756500214591
1106	48-49	-0.10060513044052755
1106	50-51	-0.09666638472421596
1106	52-53	-0.02397573001126574
1106	54-55	-0.1375099705250875
1106	56-57	-0.06924125556519556
1106	58-59	-0.09699242124121099
1106	60-61	0.004868194488260258
1106	62-63	-0.09598433321123423
1106	64-65	-0.22875289709662638
1106	66-67	-0.1536782365791467
1106	68-69	-0.06026820737899641
1106	70-71	-0.40847403852024655
1106	72-73	-0.21544487850687233
1106	74-75	-0.5055298602503058
1106	76-77	-0.3927245830657782
1106	78-79	-0.3075216024817635
1106	80-81	-0.1733510027916907
1106	82-83	-0.2796741093572308
1106	84-85	-0.3693188023348881
1106	86-87	-0.2759601470717499
1106	88-89	-0.39021885327922234
1106	90-91	-0.4621382543369492
1106	92-93	-0.4636792342667775
1106	94-95	-0.2598040754971649
1106	96-97	-0.32531830298034947
1106	98-99	-0.5890041631451837
1106	100	-0.412270092170985
1107	1	1.2055030027852762
1107	2	-0.7124177397508653
1107	3	-0.09788337364530086
1107	4	-0.22077759125482288
1107	5	-1.2453766494315595
1107	6	-0.40640148630898665
1107	7	-0.3330810499876051
1107	8	-0.39277826512520164
1107	9	-0.3549017773285996
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2224	76-77	0.8430077750518734
2224	78-79	0.45412815672379026
2224	80-81	0.4148734563544778
2224	82-83	0.2426181406222767
2224	84-85	0.3821194014930285
2224	86-87	0.35168085056635334
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2224	96-97	0.4467554879530695
2224	98-99	0.511258205262699
2224	100	0.5055305673033885
2225	1	1.223538798586798
2225	2	1.4674256046732506
2225	3	0.9977912212971365
2225	4	1.135620775114548
2225	5	3.2297880159735435
2225	6	-0.01719553070243407
2225	7	0.3672409239884118
2225	8	0.3904480556575791
2225	9	0.20811564827556595
2225	10-11	0.16727018685740802
2225	12-13	0.0699560817125473
2225	14-15	0.08420049281464514
2225	16-17	0.3038124306914298
2225	18-19	0.10982275245749662
2225	20-21	0.15978091315972165
2225	22-23	0.21725798431486965
2225	24-25	0.16253157515075856
2225	26-27	0.11161051294241275
2225	28-29	-0.009359059028028582
2225	30-31	0.07918586180930731
2225	32-33	-0.009779495951939055
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2225	44-45	0.27752379874939237
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2225	48-49	0.02525793681202515
2225	50-51	0.16068732232448468
2225	52-53	0.19634128746308477
2225	54-55	0.2396794353161198
2225	56-57	0.294894164115874
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2225	60-61	0.3425962760139143
2225	62-63	0.2811588868201369
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2225	72-73	0.39068241487717614
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2225	84-85	0.023151250858177264
2225	86-87	0.2858247997700687
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2225	90-91	0.48807849422534133
2225	92-93	0.2838086305319649
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2225	100	0.4506559568903725
2226	1	1.3152734323257533
2226	2	1.5313336223723777
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2226	4	1.0963934588671833
2226	5	3.1825498108556687
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2226	80-81	0.4498807698947189
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2226	84-85	0.39039766127537234
2226	86-87	0.31693212164472584
2226	88-89	0.3432037340335867
2226	90-91	0.5185124489887372
2226	92-93	0.4501521045501278
2226	94-95	0.7393433975986667
2226	96-97	0.6417709142305199
2226	98-99	0.553651164372944
2226	100	0.6619671575952424
2227	1	1.5550479387459184
2227	2	1.995722772441086
2227	3	0.5024599493903086
2227	4	0.006176733211750829
2227	5	3.230248177106237
2227	6	0.12306945058235996
2227	7	0.43956466379661663
2227	8	0.5385448363655243
2227	9	0.39447756905966
2227	10-11	0.4099899251234831
2227	12-13	0.38006594101052826
2227	14-15	0.3076753626522333
2227	16-17	0.3486247338265116
2227	18-19	0.18366240132355927
2227	20-21	0.22884203900949984
2227	22-23	0.2036199289415066
2227	24-25	0.17029351591491348
2227	26-27	0.32559151949362075
2227	28-29	0.34851266473796727
2227	30-31	0.3128951444037966
2227	32-33	0.20885080701767578
2227	34-35	0.20270829368883625
2227	36-37	0.31362484086113795
2227	38-39	0.3053135931338957
2227	40-41	0.35891162041883007
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2227	44-45	0.43371309711810113
2227	46-47	0.48874557149635933
2227	48-49	0.38403818078325
2227	50-51	0.3747013508015371
2227	52-53	0.3320182476720177
2227	54-55	0.4024433156713627
2227	56-57	0.43303644355385273
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2227	60-61	0.3008166254836482
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2227	64-65	0.3751929749977094
2227	66-67	0.5504054281056128
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2227	74-75	0.5186356338298594
2227	76-77	0.6155765269341416
2227	78-79	0.4469566567068952
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2227	82-83	0.292395646226872
2227	84-85	0.4687362432154458
2227	86-87	0.47274964305897527
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2227	90-91	0.4655118823249964
2227	92-93	0.5124915468472437
2227	94-95	0.6206734352785972
2227	96-97	0.36480811752703346
2227	98-99	0.5658129972478676
2227	100	0.7453853475726575
2228	1	1.3037213027302812
2228	2	1.467565152072135
2228	3	-0.17667666180937402
2228	4	-0.11573176121309992
2228	5	3.173116266760019
2228	6	-0.08862447698360398
2228	7	0.28163276926780156
2228	8	0.3603418374254801
2228	9	0.17787012118714784
2228	10-11	0.17735373877524552
2228	12-13	0.25801325750219206
2228	14-15	0.2612080193095707
2228	16-17	0.21838425633399083
2228	18-19	0.11027956146410389
2228	20-21	0.21933580705121614
2228	22-23	0.26334977453862507
2228	24-25	0.4601216512076718
2228	26-27	0.40502728687371103
2228	28-29	0.37513254952172304
2228	30-31	0.35392272550895143
2228	32-33	0.3721536243469785
2228	34-35	0.24172385839405308
2228	36-37	0.33105303325085345
2228	38-39	0.19302357150071003
2228	40-41	0.36847306859804263
2228	42-43	0.5817366099505392
2228	44-45	0.4353895966122323
2228	46-47	0.42684469160538185
2228	48-49	0.3984320127697032
2228	50-51	0.3888033083974136
2228	52-53	0.13980443406848053
2228	54-55	0.21755831008222515
2228	56-57	0.4582738733038738
2228	58-59	0.48819924029249506
2228	60-61	0.38713730104084476
2228	62-63	0.3321976919699807
2228	64-65	0.20663352521167866
2228	66-67	0.4007780290934235
2228	68-69	0.5888748549434766
2228	70-71	0.4994971166451734
2228	72-73	0.5525164875334312
2228	74-75	0.47521470683390277
2228	76-77	0.5964824442805181
2228	78-79	0.6318701143225454
2228	80-81	0.6722223859679417
2228	82-83	0.39341046732149465
2228	84-85	0.38904476927509535
2228	86-87	0.48435614092313983
2228	88-89	0.38851310471122247
2228	90-91	0.4062610193605636
2228	92-93	0.3982045787434174
2228	94-95	0.5488500317025142
2228	96-97	0.5563229275301893
2228	98-99	0.5906281072993451
2228	100	0.6169661614549327
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	13.0
13	30.0
14	107.0
15	382.0
16	1386.0
17	3476.0
18	5571.0
19	7324.0
20	8704.0
21	10027.0
22	11922.0
23	13341.0
24	15174.0
25	17555.0
26	19676.0
27	22451.0
28	25268.0
29	29165.0
30	33361.0
31	38476.0
32	45116.0
33	54279.0
34	66689.0
35	86514.0
36	121771.0
37	200647.0
38	494040.0
39	938981.0
40	1206.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.249224208275507	19.40849231355685	18.795055978113044	37.547227500054596
2	18.75348181447748	53.67098756359674	11.407942365354439	16.16758825657133
3	40.8026833848737	14.117031556085138	25.787098068688035	19.29318699035312
4	17.315893502392797	16.894227536816018	20.472206039464027	45.31767292132716
5	17.294904807247217	35.47811103503748	26.136865652990426	21.090118504724874
6	17.684317704602375	27.605634298607967	31.0505523942953	23.659495602494353
7	20.297476252413478	26.790375297229847	36.281665648766285	16.63048280159039
8	20.59633415058707	27.910564397892855	34.30586821035513	17.187233241164947
9	20.955958061330993	28.14324410424473	27.44221288609079	23.45858494833349
10-11	19.402706617643176	30.189289869280472	28.803573974370032	21.60442953870632
12-13	24.070469213940367	26.680349653180514	27.20695909448521	22.04222203839391
14-15	21.96924562141498	27.202294940008414	28.493759713321705	22.3346997252549
16-17	20.968608480312867	29.681469050254943	28.26684419787983	21.083078271552356
18-19	21.500586099411613	29.84209637023178	29.608470632547352	19.048846897809256
20-21	22.818011732548584	29.143287225672914	29.746855215844747	18.291845825933756
22-23	22.011311014620805	30.203436337811507	28.687938144511342	19.097314503056342
24-25	22.236246464482903	28.292431045316217	26.514442158324286	22.95688033187659
26-27	20.048450004664154	26.29104675946867	30.237119453396293	23.423383782470875
28-29	19.671052747372663	27.91240071036203	30.665031566756607	21.751514975508705
30-31	19.970322674902157	30.291048132361574	28.122653955524495	21.615975237211774
32-33	20.733464692350612	30.120537592205054	28.69473636966856	20.451261345775773
34-35	21.325174289772477	28.983253925370008	30.10513708214016	19.586434702717355
36-37	22.172686359372225	27.99174708666351	28.643826683539757	21.19173987042451
38-39	21.542860034409472	29.324175074693198	28.822773614710712	20.31019127618662
40-41	20.81261014294106	29.701181238353175	29.859677068047162	19.6265315506586
42-43	20.712309105093567	29.91880407506476	29.380358004814646	19.98852881502703
44-45	20.78435237775075	29.94050562954645	28.5844247161466	20.6907172765562
46-47	20.10646325928501	27.90061229024714	28.66869223339633	23.324232217071522
48-49	21.035556697637826	28.190413666500636	30.29546538581358	20.478564250047963
50-51	22.043146068997803	30.77228717815134	28.052007082474574	19.132559670376285
52-53	20.450876775734052	30.229627392039088	28.277648252575833	21.041847579651034
54-55	22.255889855348006	28.434978778388636	28.110119126208293	21.199012240055065
56-57	21.463180650520098	28.615843294296262	29.386390015017078	20.534586040166563
58-59	21.131769914230517	27.939310059541363	29.055215479873436	21.873704546354684
60-61	19.589953917172352	30.405689928968293	29.693172762861984	20.31118339099737
62-63	20.413165204472485	31.17545274038191	27.00577895285304	21.40560310229257
64-65	19.92706752029569	30.08545090533078	28.920709304116336	21.06677227025719
66-67	20.26350712735606	30.051653310757654	29.54981668992877	20.13502287195752
68-69	20.96544037538523	29.932563366498698	29.190786798858774	19.91120945925729
70-71	19.611471531937138	30.780141438284435	29.59256410572318	20.015822924055247
72-73	20.234334161147416	30.67770604562423	28.465180766786997	20.622779026441357
74-75	20.07962503718123	29.07378692382292	30.457192742223622	20.38939529677223
76-77	19.803691898275673	30.138688193352962	28.67293364756241	21.38468626080896
78-79	19.52764875572679	29.4016637831045	28.410794965529256	22.659892495639454
80-81	20.722218555430885	29.34422370360592	28.41788220799507	21.51567553296812
82-83	19.994724225266342	28.31583982061486	30.229287193991865	21.460148760126934
84-85	20.297938267715427	29.100407805506517	28.532943011072526	22.068710915705527
86-87	21.858185943118436	28.641362601929377	28.82885281160512	20.671598643347068
88-89	21.87853661713276	28.85206358034578	28.579298546367855	20.690101256153604
90-91	21.73115373581173	29.567197265573437	28.523020682445004	20.17862831616983
92-93	21.333534566664845	29.516287579444633	29.749803313485746	19.400374540404776
94-95	22.19556711718292	29.314078882292584	28.587548819616902	19.902805180907592
96-97	21.152226561743724	29.554722852420873	29.357882333062875	19.93516825277253
98-99	22.359890559575334	29.591794080219934	28.62255197892154	19.425763381283186
100	22.742813241974574	30.97244980753763	27.24416232665626	19.040574623831542
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	11.0
2	14.0
3	17.0
4	26.0
5	25.5
6	33.5
7	47.5
8	45.0
9	45.5
10	62.5
11	78.0
12	78.5
13	80.5
14	115.5
15	158.5
16	192.5
17	268.0
18	393.0
19	654.5
20	1049.5
21	1567.0
22	2408.0
23	3807.5
24	5921.5
25	8718.0
26	12391.5
27	16892.0
28	21870.0
29	27291.5
30	33617.0
31	41040.0
32	49058.0
33	58212.5
34	68200.0
35	78853.0
36	89553.5
37	97507.5
38	103177.5
39	107319.0
40	108962.5
41	108716.0
42	107442.0
43	105875.0
44	105542.5
45	103409.5
46	96550.0
47	88127.5
48	80270.5
49	75731.5
50	73899.5
51	70289.0
52	63214.0
53	54273.0
54	44671.0
55	34919.5
56	26355.0
57	19281.5
58	13670.5
59	9559.5
60	7320.0
61	6035.5
62	4897.5
63	3849.0
64	2991.5
65	3066.5
66	4333.5
67	4289.0
68	2548.0
69	2159.5
70	3101.0
71	3073.5
72	1677.0
73	578.5
74	261.5
75	136.5
76	79.0
77	62.5
78	60.5
79	57.5
80	50.5
81	34.5
82	19.5
83	16.5
84	15.5
85	16.5
86	17.0
87	15.0
88	12.0
89	11.5
90	10.5
91	7.5
92	7.5
93	8.5
94	14.0
95	14.5
96	9.0
97	14.0
98	26.0
99	67.5
100	87.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.349559897423802
2	0.005720189452674673
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	3.520116586261337E-4
30-31	0.009240306038936011
32-33	0.0
34-35	0.0
36-37	0.0
38-39	9.680320612218676E-4
40-41	0.0016280539211458683
42-43	4.4001457328266715E-5
44-45	0.0
46-47	0.0016280539211458683
48-49	0.0
50-51	0.0
52-53	0.0015620517351534683
54-55	0.019360641224437355
56-57	0.010538349030119878
58-59	0.012408410966571214
60-61	2.2000728664133356E-4
62-63	0.011792390563975479
64-65	8.800291465653343E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	8.800291465653343E-5
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2272652.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.25238556208377
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.65771016346326	64.34541706984778
2	8.300279928256492	12.32631171012252
3	2.2797244078746095	5.078249271263958
4	0.9609709560444938	2.8541754376872
5	0.4853979066527186	1.8020976257903005
6	0.3251939946770232	1.4487857925139525
7	0.20605985026011744	1.071030481526564
8	0.1448435202707005	0.8603981530647639
9	0.10750931985572493	0.7184541122500209
>10	0.4951633626573771	6.32859613138095
>50	0.024319955186921424	1.2207245494223782
>100	0.012102014019163615	1.5473349607854308
>500	7.246207814905003E-4	0.3984247043442852
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	2.2000728664133358E-5
20-21	0.0	0.0	0.0	0.0	4.4001457328266715E-5
22-23	0.0	0.0	4.4001457328266715E-5	0.0	4.4001457328266715E-5
24-25	0.0	0.0	4.4001457328266715E-5	0.0	4.4001457328266715E-5
26-27	0.0	0.0	4.4001457328266715E-5	0.0	4.4001457328266715E-5
28-29	0.0	0.0	4.4001457328266715E-5	0.0	4.4001457328266715E-5
30-31	0.0	0.0	4.4001457328266715E-5	0.0	4.4001457328266715E-5
32-33	0.0	0.0	4.4001457328266715E-5	0.0	4.4001457328266715E-5
34-35	0.0	0.0	4.4001457328266715E-5	0.0	4.4001457328266715E-5
36-37	0.0	0.0	4.4001457328266715E-5	0.0	4.4001457328266715E-5
38-39	0.0	0.0	4.4001457328266715E-5	0.0	4.4001457328266715E-5
40-41	0.0	0.0	4.4001457328266715E-5	0.0	4.4001457328266715E-5
42-43	0.0	0.0	4.4001457328266715E-5	0.0	4.4001457328266715E-5
44-45	0.0	0.0	4.4001457328266715E-5	0.0	4.4001457328266715E-5
46-47	0.0	0.0	4.4001457328266715E-5	0.0	4.4001457328266715E-5
48-49	2.2000728664133358E-5	0.0	4.4001457328266715E-5	0.0	4.4001457328266715E-5
50-51	8.800291465653343E-5	0.0	4.4001457328266715E-5	0.0	4.4001457328266715E-5
52-53	1.1000364332066678E-4	0.0	4.4001457328266715E-5	0.0	4.4001457328266715E-5
54-55	1.3200437198480013E-4	0.0	4.4001457328266715E-5	0.0	4.4001457328266715E-5
56-57	1.3200437198480013E-4	0.0	3.08010201297867E-4	0.0	4.4001457328266715E-5
58-59	1.540051006489335E-4	0.0	5.720189452674673E-4	0.0	4.4001457328266715E-5
60-61	2.2000728664133356E-4	0.0	5.720189452674673E-4	0.0	4.4001457328266715E-5
62-63	2.2000728664133356E-4	0.0	5.720189452674673E-4	0.0	4.4001457328266715E-5
64-65	2.2000728664133356E-4	0.0	5.720189452674673E-4	0.0	4.4001457328266715E-5
66-67	3.08010201297867E-4	0.0	5.720189452674673E-4	0.0	4.4001457328266715E-5
68-69	5.060167592750672E-4	0.0	5.720189452674673E-4	0.0	4.4001457328266715E-5
70-71	6.16020402595734E-4	0.0	5.720189452674673E-4	0.0	4.4001457328266715E-5
72-73	7.480247745805341E-4	0.0	5.720189452674673E-4	0.0	4.4001457328266715E-5
74-75	7.700255032446674E-4	0.0	5.720189452674673E-4	0.0	4.4001457328266715E-5
76-77	7.920262319088008E-4	0.0	5.720189452674673E-4	0.0	4.4001457328266715E-5
78-79	9.680320612218676E-4	0.0	5.720189452674673E-4	0.0	4.4001457328266715E-5
80-81	0.001364045177176268	0.0	5.720189452674673E-4	0.0	4.4001457328266715E-5
82-83	0.0015620517351534681	0.0	5.720189452674673E-4	0.0	4.4001457328266715E-5
84-85	0.0016940561071382685	0.0	5.720189452674673E-4	0.0	4.4001457328266715E-5
86-87	0.0017600582931306685	0.0	5.720189452674673E-4	0.0	4.4001457328266715E-5
88	0.0017600582931306685	0.0	5.720189452674673E-4	0.0	4.4001457328266715E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	8705	0.0	79.96631	8
GGCTGCC	40	4.271351E-8	79.42136	1
GGCGCGC	575	0.0	76.735214	6
AGGCGCG	585	0.0	74.62112	5
CTTCACC	9855	0.0	74.111855	6
CACCGGC	8935	0.0	73.86275	9
CCTTCAC	10845	0.0	71.11196	5
GTGTGGC	3720	0.0	71.03954	7
GCAGCCT	45	9.661744E-8	70.59676	1
CGGGGCA	935	0.0	69.78111	94
CGTAGTG	1700	0.0	69.5803	4
GCGCGCA	645	0.0	69.13509	7
AGCTGCC	85	0.0	66.271324	2
TTCACCG	10770	0.0	65.98494	7
TGTGGCA	4245	0.0	62.917175	8
GCTGCCC	90	0.0	62.585453	3
CAGCGTA	880	0.0	61.371056	1
GCCTTCA	3225	0.0	60.693336	4
GAGCAGC	35	1.630569E-4	60.511513	1
CGGGCCC	510	0.0	59.82433	7
>>END_MODULE