FastQCFastQC Report
Tue 7 Mar 2017
1NF_27A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_27A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2272652
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG87050.079.966318
GGCTGCC404.271351E-879.421361
GGCGCGC5750.076.7352146
AGGCGCG5850.074.621125
CTTCACC98550.074.1118556
CACCGGC89350.073.862759
CCTTCAC108450.071.111965
GTGTGGC37200.071.039547
GCAGCCT459.661744E-870.596761
CGGGGCA9350.069.7811194
CGTAGTG17000.069.58034
GCGCGCA6450.069.135097
AGCTGCC850.066.2713242
TTCACCG107700.065.984947
TGTGGCA42450.062.9171758
GCTGCCC900.062.5854533
CAGCGTA8800.061.3710561
GCCTTCA32250.060.6933364
GAGCAGC351.630569E-460.5115131
CGGGCCC5100.059.824337
Produced by FastQC (version 0.11.5)