FastQCFastQC Report
Tue 7 Mar 2017
1NF_28A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_28A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2344763
Sequences flagged as poor quality0
Sequence length95
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA122950.5243600312696849No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG72210.30796289433089824No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC69410.2960213889420807No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA56430.24066398181820506No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA55720.23763595723746922No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG55010.2346079326567333No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA54990.23452263618967034No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA52660.22458559777683287No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT49470.21098081128028714No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG47160.20112906933451272No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG43660.18620218759849075No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG43210.18428301708957365No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA40690.17353566223963787No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG38860.16573103550337498No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT37280.15899261460539935No Hit
CAGCAGGCGCGCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACGAAA34490.1470937574501133No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA34130.1455584210429796No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC33850.14436427050409784No Hit
CAGCAGAAGAGCGGCCGGGAGAGTTGAGGGTGGACGTCTCGAGCTCGAGG33550.14308482349815313No Hit
CAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTTGGCACTCTCATGCGA31900.13604786496545707No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT31170.13293454391765822No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA30120.12845647939685162No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA29060.12393576664251355No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA28590.12193129966653346No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACTTCCGAGCTCA28190.1202253703252738No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA27820.11864738568460864No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC26880.11463845173264847No Hit
CAGCTGCCCGCCGACGAGCTGAATTCTCCGGGCCGACTTTGCGGGTCCAC26490.1129751706249203No Hit
CAGCCCAAAGTGGGTGGTAAACTCCATCTAAGGCTAAATACTTCCCCGAG24800.10576761915809829No Hit
CAGCGGTTCTGACGTGCAAATCGATCGTCAAACTTGAGCATAGGGGCGAA24750.10555437799044083No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC24500.10448817215215353No Hit
CTGCCATTGCCTACTGTAAGGGGCAGTGGGTCGGCGGAGACCGGCGACTG24020.10244105694264195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCA355.5479177E-1089.000013
GCTGCAC355.5479177E-1089.000013
GCTGCTT301.9386789E-889.03
GCTGCTA256.81599E-789.03
GCTGCGT500.089.03
TGCTGCA550.089.02
AGCTGCC3350.089.02
GCAGCCC256.81599E-789.03
GCAGCAC600.089.03
GCAGCAA1100.089.03
AGCAGCG1000.089.02
AGCAGCC1150.089.02
AGCAGCA2450.089.02
CAGCTGC7800.088.429481
CAGCAGC5250.086.457141
GCTGCCC3300.086.303033
AGCTGCG1450.085.931032
AGCTGCA1950.084.43592
CTGCTGC1600.083.437511
CAGCCCT135650.082.7342451
Produced by FastQC (version 0.11.5)