FastQCFastQC Report
Tue 7 Mar 2017
1NF_28A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_28A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2344763
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGC7500.080.733696
TCACCGG94150.079.418528
CCGCTGC200.001349584979.401431
GCGCGCA8000.075.6878367
CACCGGC95650.074.6398249
CTTCACC105650.073.928216
CGCTGCA800.072.7846451
AGGCGCG8400.072.642445
CCTTCAC114800.071.715675
GGCTGCG459.678661E-870.579051
GTGTGGC37000.070.407297
CGTAGTG15850.067.2237854
TTCACCG116050.066.898557
CGGGGCA11950.065.2028494
CAGCTGC900.064.6974641
AACGGGC6200.064.3507465
TGTGGCA41550.063.826918
CGCGCAA9500.063.7371258
AGGCTGC603.6561687E-1062.5882572
CGGGCCC6450.061.8565337
Produced by FastQC (version 0.11.5)