FastQCFastQC Report
Tue 7 Mar 2017
1NF_29A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_29A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3829981
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG160550.081.7425848
CACCGGC164400.078.086699
CTTCACC179400.075.299116
CCTTCAC198500.071.647935
GTGTGGC58800.071.204767
TTCACCG197550.068.167147
CGTAGTG29550.066.395754
CGGGCCC11200.064.539297
GGCGCGC5850.063.3859146
CGGGGCA16450.062.77378594
ACCTTCA77700.062.4627384
AACGGGC11800.060.859865
CAGCTGC1400.060.504831
TGTGGCA69200.060.503478
GCCTTCA56600.060.372124
GGGCCCG12300.059.5307048
GGCCCGA12150.059.1066979
CAGCGTA13000.058.643141
ACGGGCC12500.057.451716
AGGCGCG6450.056.761835
Produced by FastQC (version 0.11.5)