##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1NF_29A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3829981
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.817362801538701	12.0	12.0	12.0	2.0	32.0
2	14.648082327301363	12.0	12.0	12.0	12.0	22.0
3	13.728056875477971	12.0	12.0	12.0	12.0	22.0
4	13.708708215523785	12.0	12.0	12.0	12.0	22.0
5	22.427021700629847	22.0	22.0	22.0	22.0	27.0
6	26.760402988944332	27.0	22.0	32.0	22.0	32.0
7	34.9113765838525	37.0	37.0	37.0	27.0	41.0
8	37.53987291320767	41.0	37.0	41.0	27.0	41.0
9	38.4992358969927	41.0	37.0	41.0	32.0	41.0
10-11	38.855402285285486	41.0	41.0	41.0	34.5	41.0
12-13	39.03895463188982	41.0	41.0	41.0	37.0	41.0
14-15	38.99600311333137	41.0	41.0	41.0	37.0	41.0
16-17	38.89450091266771	41.0	41.0	41.0	37.0	41.0
18-19	38.83219002914113	41.0	41.0	41.0	37.0	41.0
20-21	38.84603148161831	41.0	41.0	41.0	37.0	41.0
22-23	38.83654488103204	41.0	41.0	41.0	34.5	41.0
24-25	38.86202607793616	41.0	41.0	41.0	37.0	41.0
26-27	38.829675656354425	41.0	41.0	41.0	37.0	41.0
28-29	38.77277250200458	41.0	41.0	41.0	34.5	41.0
30-31	38.65443601417344	41.0	41.0	41.0	32.0	41.0
32-33	38.69537733999202	41.0	41.0	41.0	32.0	41.0
34-35	38.66838099196837	41.0	41.0	41.0	32.0	41.0
36-37	38.58486412856878	41.0	41.0	41.0	32.0	41.0
38-39	38.47732612772752	41.0	41.0	41.0	32.0	41.0
40-41	38.53525683286679	41.0	41.0	41.0	32.0	41.0
42-43	38.27396532776534	41.0	41.0	41.0	32.0	41.0
44-45	38.38693533988811	41.0	41.0	41.0	32.0	41.0
46-47	38.395231595143684	41.0	41.0	41.0	32.0	41.0
48-49	38.33561759183662	41.0	41.0	41.0	32.0	41.0
50-51	38.217952386708966	41.0	41.0	41.0	32.0	41.0
52-53	38.20129251293936	41.0	41.0	41.0	32.0	41.0
54-55	38.06818741398456	41.0	37.0	41.0	32.0	41.0
56-57	37.84573239397271	41.0	37.0	41.0	27.0	41.0
58-59	37.845723647193026	41.0	37.0	41.0	29.5	41.0
60-61	37.844490612355514	41.0	37.0	41.0	29.5	41.0
62-63	37.84378786735496	41.0	37.0	41.0	29.5	41.0
64-65	37.82775854501628	41.0	37.0	41.0	29.5	41.0
66-67	37.68931843265018	41.0	37.0	41.0	27.0	41.0
68-69	37.71304048244626	41.0	37.0	41.0	27.0	41.0
70-71	37.69566271999783	41.0	37.0	41.0	27.0	41.0
72-73	37.53502837220341	41.0	37.0	41.0	27.0	41.0
74-75	37.46347971439023	41.0	37.0	41.0	27.0	41.0
76-77	36.4243286063299	39.0	34.5	41.0	24.5	41.0
78-79	36.93058020392269	41.0	37.0	41.0	27.0	41.0
80-81	37.59611692590642	41.0	37.0	41.0	27.0	41.0
82-83	37.7790337602197	41.0	37.0	41.0	27.0	41.0
84-85	37.69348803558033	41.0	37.0	41.0	27.0	41.0
86-87	37.562251483754096	41.0	37.0	41.0	27.0	41.0
88-89	37.47473681984323	41.0	37.0	41.0	27.0	41.0
90-91	37.31714060722494	41.0	37.0	41.0	27.0	41.0
92-93	37.19902135284744	41.0	37.0	41.0	27.0	41.0
94-95	37.04542294074044	41.0	37.0	41.0	27.0	41.0
96-97	36.97921882641194	41.0	37.0	41.0	27.0	41.0
98-99	36.86404932557107	41.0	37.0	41.0	27.0	41.0
100	36.72510255272807	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.4691855319259073
1101	2	-0.3996783303366218
1101	3	-0.07268244967092308
1101	4	0.11063485463743739
1101	5	-1.1051353686624026
1101	6	-0.6501087172116797
1101	7	-0.22153193486731482
1101	8	-0.14446743517471106
1101	9	-0.14608329588231328
1101	10-11	-0.06482311737267565
1101	12-13	-0.07962618729158777
1101	14-15	-0.12817691729866
1101	16-17	-0.05568043191532013
1101	18-19	0.04530834565131414
1101	20-21	0.054810745397269045
1101	22-23	0.031229908792013816
1101	24-25	-0.004899141307042498
1101	26-27	0.06128505894291436
1101	28-29	-0.044751641431339806
1101	30-31	-0.08041401141035465
1101	32-33	-0.07019092285232631
1101	34-35	0.12702111486529333
1101	36-37	0.02969042149531731
1101	38-39	0.04504668064052453
1101	40-41	-0.041258261956606646
1101	42-43	0.14657962298840488
1101	44-45	0.003394549466698038
1101	46-47	-0.03342216875245896
1101	48-49	0.03551790604516469
1101	50-51	0.14522717107205807
1101	52-53	-0.04568885418911606
1101	54-55	0.041400959861512376
1101	56-57	0.12746969239293549
1101	58-59	0.0887967286303919
1101	60-61	0.0668087390583807
1101	62-63	-0.0693401637278157
1101	64-65	-0.11602442632690213
1101	66-67	-0.037832364524170714
1101	68-69	-0.019391925894829853
1101	70-71	-0.03794256657415929
1101	72-73	0.07241996161716457
1101	74-75	0.09080976933118023
1101	76-77	-0.3967315547313248
1101	78-79	-0.21733311192003413
1101	80-81	0.03855966215640905
1101	82-83	-0.10676397983034036
1101	84-85	-0.008037654690582485
1101	86-87	-0.1465072391129283
1101	88-89	-0.31979563188601645
1101	90-91	-0.1570231196740579
1101	92-93	0.016543407314479452
1101	94-95	0.0447572938040679
1101	96-97	-0.17884446656686492
1101	98-99	-0.39454445399802296
1101	100	-0.5150261899969664
1102	1	1.6660515558376616
1102	2	0.8774844168748981
1102	3	-0.17879259142240045
1102	4	0.06134639107576412
1102	5	-0.8423958987863287
1102	6	-0.2038287754129584
1102	7	-0.18485242501260046
1102	8	-0.4150259649770618
1102	9	-0.44757862409822025
1102	10-11	-0.459234047779276
1102	12-13	-0.45464132319713
1102	14-15	-0.42777157278559486
1102	16-17	-0.4792098670333971
1102	18-19	-0.36381269990726395
1102	20-21	-0.2878512683431538
1102	22-23	-0.14946302785156718
1102	24-25	-0.25528274966722364
1102	26-27	-0.24115911685988323
1102	28-29	-0.4011272818538387
1102	30-31	-0.34909965899883844
1102	32-33	-0.44506512114558916
1102	34-35	-0.1916656244934174
1102	36-37	-0.2506394519188859
1102	38-39	-0.2313964907336583
1102	40-41	-0.38277490946767045
1102	42-43	-0.3662943331303623
1102	44-45	-0.2534940244630164
1102	46-47	-0.5430595223808083
1102	48-49	-0.34150211702095845
1102	50-51	-0.4199882351954969
1102	52-53	-0.35542804020492724
1102	54-55	-0.31467990259728396
1102	56-57	-0.23960827389716854
1102	58-59	-0.2972562043607212
1102	60-61	-0.2856931020632416
1102	62-63	-0.4448733942362395
1102	64-65	-0.4319440243966497
1102	66-67	-0.2686325924063979
1102	68-69	-0.6191152117659371
1102	70-71	-0.6212813587586723
1102	72-73	-0.43987833383175
1102	74-75	-0.5193806155476111
1102	76-77	-0.13404766588544703
1102	78-79	-0.4200043759319101
1102	80-81	-0.5608569355302251
1102	82-83	-0.4298803287918673
1102	84-85	-0.41546149379144026
1102	86-87	-0.28541217371589056
1102	88-89	-0.5515685277966398
1102	90-91	-0.6283601809064834
1102	92-93	-0.3420693695733448
1102	94-95	-0.2246389969561733
1102	96-97	-0.32765274972839364
1102	98-99	-0.45811922735644117
1102	100	-0.6567856075077643
1103	1	1.5963389112768063
1103	2	0.6739012737984194
1103	3	0.44571874283839286
1103	4	1.1713147185749726
1103	5	2.892046847799623
1103	6	-0.016584953023627946
1103	7	-0.40122932552656465
1103	8	-0.20303451528584304
1103	9	-0.32601480032209906
1103	10-11	-0.13791260670070216
1103	12-13	-0.32502886875391823
1103	14-15	-0.2796283296950506
1103	16-17	-0.3085402841577931
1103	18-19	-0.3405755858403694
1103	20-21	-0.27883107700039744
1103	22-23	-0.266434819538965
1103	24-25	-0.3184515733566684
1103	26-27	-0.27175699088716954
1103	28-29	-0.2140792625774779
1103	30-31	-0.16424207477632535
1103	32-33	-0.34921662317552205
1103	34-35	-0.4425421353322392
1103	36-37	-0.3656314765347517
1103	38-39	-0.30510109371455485
1103	40-41	-0.2861683031604514
1103	42-43	-0.22989483082954365
1103	44-45	-0.44868031271231956
1103	46-47	-0.4756265919078899
1103	48-49	-0.3887247531542002
1103	50-51	-0.18163944105076268
1103	52-53	-0.3590120904426186
1103	54-55	-0.4276629244015666
1103	56-57	-0.29923346542270224
1103	58-59	-0.4265376796886713
1103	60-61	-0.35622542516029654
1103	62-63	-0.32400309969716545
1103	64-65	-0.495522687354196
1103	66-67	-0.7144452485268218
1103	68-69	-0.5451186555654601
1103	70-71	-0.596063040533167
1103	72-73	-0.5917675239999554
1103	74-75	-0.6238629529840907
1103	76-77	-0.5691263145170353
1103	78-79	-0.5304216584128412
1103	80-81	-0.15606323521695487
1103	82-83	-0.3790157547575035
1103	84-85	-0.6991083052140965
1103	86-87	-0.3773027093568615
1103	88-89	-0.23702214388045206
1103	90-91	-0.6770137515783361
1103	92-93	-0.4041180288025501
1103	94-95	-0.44167022117562027
1103	96-97	-0.25111362723351505
1103	98-99	-0.367872629502898
1103	100	-0.24160477659660273
1104	1	1.605433674726049
1104	2	-0.6149338165909164
1104	3	0.07390681279108513
1104	4	0.03664242081129032
1104	5	-1.089246368593411
1104	6	-0.4230984926547805
1104	7	-0.10476988581459068
1104	8	-0.1160916161857557
1104	9	0.023699420806600813
1104	10-11	-0.08942411288689556
1104	12-13	-0.06247563358347463
1104	14-15	-0.23128735050553217
1104	16-17	0.053681193261681415
1104	18-19	0.09993015544518613
1104	20-21	0.06189453478715734
1104	22-23	0.06836914025952012
1104	24-25	-0.020568176159478924
1104	26-27	0.03779521350094228
1104	28-29	-0.033589204617236135
1104	30-31	0.015784372293452975
1104	32-33	0.03899677633948784
1104	34-35	-0.15422725922405078
1104	36-37	-0.14405700317151826
1104	38-39	-0.12631846520415735
1104	40-41	-0.10084120335893942
1104	42-43	-0.07768726674190418
1104	44-45	-0.08241947354976986
1104	46-47	-0.24854998686277696
1104	48-49	-0.30734031611996926
1104	50-51	-0.17008074656520478
1104	52-53	-0.014298624177470742
1104	54-55	-0.1520417681653825
1104	56-57	-0.12024144076764998
1104	58-59	-0.17712193679329147
1104	60-61	-0.07011967382809559
1104	62-63	-0.0931314284110698
1104	64-65	-0.16776430265844056
1104	66-67	-0.4023696431412773
1104	68-69	-0.328409389235496
1104	70-71	-0.33501466383559375
1104	72-73	-0.0444140100714705
1104	74-75	-0.25867644071667684
1104	76-77	-0.23064850345569
1104	78-79	-0.32532414087193473
1104	80-81	-0.16402060722162304
1104	82-83	-0.3353347725594489
1104	84-85	-0.23409334126144188
1104	86-87	-0.18698477393551372
1104	88-89	-0.2123714582377545
1104	90-91	-0.2618957230434944
1104	92-93	-0.254614505150478
1104	94-95	-0.24841541609831097
1104	96-97	-0.22823061452077553
1104	98-99	-0.2504953066594453
1104	100	-0.22313405278938347
1105	1	1.6342378568313762
1105	2	0.20933837905379882
1105	3	-0.12969953300258474
1105	4	-0.14196015172869103
1105	5	-1.1370359514699615
1105	6	0.595326986176147
1105	7	0.3257278324071464
1105	8	0.09767794352480763
1105	9	0.12523239983221401
1105	10-11	0.12803289895722259
1105	12-13	-0.04608372606730882
1105	14-15	0.06611170170921099
1105	16-17	0.03857933117959789
1105	18-19	0.03032779375191552
1105	20-21	0.12082728257342268
1105	22-23	0.13110194650821683
1105	24-25	0.02800960722063195
1105	26-27	0.05581130635128062
1105	28-29	0.1031047173652766
1105	30-31	0.11175229486304517
1105	32-33	-0.08956421814214366
1105	34-35	0.16961049761233227
1105	36-37	0.03206214212060843
1105	38-39	0.050194386151893866
1105	40-41	0.02406711511716253
1105	42-43	0.10784087971587297
1105	44-45	0.0879729106709135
1105	46-47	-0.020957617043094956
1105	48-49	0.04169313965395105
1105	50-51	0.10125986090631045
1105	52-53	0.10014080960409899
1105	54-55	0.09346140255028246
1105	56-57	0.1836791428195781
1105	58-59	0.2955029434502521
1105	60-61	0.0891524959754193
1105	62-63	-0.144725362017752
1105	64-65	0.09742863871609586
1105	66-67	-0.018139504712138432
1105	68-69	-0.15836086821516204
1105	70-71	-0.06063241532328334
1105	72-73	-0.09225654551574536
1105	74-75	-0.12188116865829102
1105	76-77	-0.11636091959331907
1105	78-79	0.09257888138382953
1105	80-81	-0.1775274538703755
1105	82-83	-0.007820995037143064
1105	84-85	-0.041640847248061164
1105	86-87	0.015110737429935739
1105	88-89	-0.046825263232328496
1105	90-91	-0.09743936984347812
1105	92-93	0.09451565804241824
1105	94-95	0.16810685371940792
1105	96-97	0.12769744952546347
1105	98-99	-0.010958913616676114
1105	100	-0.023317253782181524
1106	1	1.446423654241734
1106	2	-0.6558613989900035
1106	3	-0.03089571265010882
1106	4	-0.2630895178917534
1106	5	-1.1844840728721273
1106	6	-0.7939220935431202
1106	7	-0.262564804554664
1106	8	-0.13443718947979022
1106	9	-0.25405395459004865
1106	10-11	-0.2286743622701053
1106	12-13	-0.29511219911482556
1106	14-15	-0.2123916973678135
1106	16-17	-0.15705275282228826
1106	18-19	-0.15638242126836843
1106	20-21	-0.17152609977510025
1106	22-23	-0.18953415241990257
1106	24-25	-0.26572316320797285
1106	26-27	-0.23484324036419224
1106	28-29	-0.3688197660681354
1106	30-31	-0.10614041455060885
1106	32-33	-0.1799206336875585
1106	34-35	-0.248418169770396
1106	36-37	-0.45468996444836307
1106	38-39	-0.2749980683256581
1106	40-41	-0.35734261345167084
1106	42-43	-0.1367633789089311
1106	44-45	-0.2749679442046471
1106	46-47	-0.3561932231384901
1106	48-49	-0.41928156430859786
1106	50-51	-0.27429829903829983
1106	52-53	-0.32478790788319856
1106	54-55	-0.302346980377159
1106	56-57	-0.17444277820057863
1106	58-59	-0.31917800388661277
1106	60-61	-0.271613099552944
1106	62-63	-0.4227565120519472
1106	64-65	-0.5296983669685247
1106	66-67	-0.39766317641905147
1106	68-69	-0.4674318070416561
1106	70-71	-0.532866889401177
1106	72-73	-0.5416656633898
1106	74-75	-0.6396642034926927
1106	76-77	-0.39486580962438467
1106	78-79	-0.34857938202447514
1106	80-81	-0.38129188279109627
1106	82-83	-0.3922947193576931
1106	84-85	-0.6431864894833268
1106	86-87	-0.493227359146573
1106	88-89	-0.5815427670583304
1106	90-91	-0.56348110241386
1106	92-93	-0.5239196393658503
1106	94-95	-0.47836110597643255
1106	96-97	-0.4613043703756361
1106	98-99	-0.6996539718537633
1106	100	-0.8212802016147194
1107	1	1.5482830805515952
1107	2	-0.4942483079762159
1107	3	-0.0520773440375315
1107	4	-0.1998150863666286
1107	5	-1.2273372791462052
1107	6	-0.01485230701072382
1107	7	-0.21454083655324752
1107	8	-0.15887180375470678
1107	9	-0.17112362930818392
1107	10-11	-0.05434464515760595
1107	12-13	-0.2064453872221037
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2222	4	0.7623434254883001
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2223	1	1.3486760042973938
2223	2	-0.8922923113983874
2223	3	0.019746971420977033
2223	4	-0.05978382422568984
2223	5	-1.0021759602376399
2223	6	0.06277904436263526
2223	7	0.19360314447528282
2223	8	0.38973172424478975
2223	9	0.4006243014183113
2223	10-11	0.25256210850154304
2223	12-13	0.27442258968079614
2223	14-15	0.3086237530509024
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2223	22-23	0.34497642735046696
2223	24-25	0.2553358885153614
2223	26-27	0.27109957120602246
2223	28-29	0.2043660959082132
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2223	32-33	0.23615480153081592
2223	34-35	0.1979851052131565
2223	36-37	0.21463307421191047
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2223	40-41	0.1288635651348926
2223	42-43	0.32442557358843516
2223	44-45	0.3674376167149944
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2223	48-49	0.29653476059166906
2223	50-51	0.2949470477312417
2223	52-53	0.3460476377503454
2223	54-55	0.3554828889299344
2223	56-57	0.4013729109699611
2223	58-59	0.5059425030557136
2223	60-61	0.43918411393266155
2223	62-63	0.49645699777655494
2223	64-65	0.3248442751074023
2223	66-67	0.47986807482654825
2223	68-69	0.49616862976184706
2223	70-71	0.477000544132693
2223	72-73	0.5162541078551328
2223	74-75	0.5168057423845127
2223	76-77	0.4578669368306265
2223	78-79	0.388593593474873
2223	80-81	0.32549594476738974
2223	82-83	0.3264744271650599
2223	84-85	0.3640677669205985
2223	86-87	0.46556196590859855
2223	88-89	0.5769425093234943
2223	90-91	0.39882395098154433
2223	92-93	0.4063068368263245
2223	94-95	0.5297219536259234
2223	96-97	0.5554546340716087
2223	98-99	0.5180782036217693
2223	100	0.5258319288316358
2224	1	1.2018535479191765
2224	2	-0.8633787063945046
2224	3	0.028371948981229522
2224	4	-0.34163124330749817
2224	5	-1.018110063585457
2224	6	0.590155035162212
2224	7	0.5052553172535639
2224	8	0.11771747389633447
2224	9	0.184458798885899
2224	10-11	0.32747757359951635
2224	12-13	0.23305030267353288
2224	14-15	0.1681625992873066
2224	16-17	0.1586726032615644
2224	18-19	0.10539659814251223
2224	20-21	0.10421943289358637
2224	22-23	0.22435017057818385
2224	24-25	0.19599105721960797
2224	26-27	0.15744653413145926
2224	28-29	0.19696261024781592
2224	30-31	0.2122881948934605
2224	32-33	0.1363458970037712
2224	34-35	0.09593180761164888
2224	36-37	0.019615016174903133
2224	38-39	-0.03799316724762036
2224	40-41	0.17505958237866537
2224	42-43	0.32820498764373696
2224	44-45	0.1916840719876589
2224	46-47	0.2452524668544882
2224	48-49	0.1458996383412554
2224	50-51	0.20911369362423216
2224	52-53	0.206633937131798
2224	54-55	0.1694973627975216
2224	56-57	0.27005036031552976
2224	58-59	0.20471810005339108
2224	60-61	0.18700609901343057
2224	62-63	0.27919761503400764
2224	64-65	0.18537189235163964
2224	66-67	0.17057954157548494
2224	68-69	0.32389987544433296
2224	70-71	0.3579056694631859
2224	72-73	0.3653011484267026
2224	74-75	0.3301808611917849
2224	76-77	0.4455292579797643
2224	78-79	0.20193834647201214
2224	80-81	0.1744516369514244
2224	82-83	0.1570493514973066
2224	84-85	0.013033355179949524
2224	86-87	0.2514973926368995
2224	88-89	0.21918688064832992
2224	90-91	0.2546714691077696
2224	92-93	0.048419915757186516
2224	94-95	0.39739406879448325
2224	96-97	0.3660498173347122
2224	98-99	0.18299420744099848
2224	100	0.4721682645600538
2225	1	1.3888265668221997
2225	2	1.623745399277409
2225	3	0.8898334264640209
2225	4	1.1424849535628354
2225	5	3.1369818035386317
2225	6	-0.05487619614048356
2225	7	0.009113686186843495
2225	8	0.2774729194724159
2225	9	0.19728650969091177
2225	10-11	0.14206058920092346
2225	12-13	0.05935699792070892
2225	14-15	0.16448565179309327
2225	16-17	0.0722691136013296
2225	18-19	-0.062027904561638536
2225	20-21	0.04068237683143394
2225	22-23	0.010740797332694285
2225	24-25	0.18023248935123348
2225	26-27	0.13411494099145926
2225	28-29	-0.022278034187948492
2225	30-31	-0.04087792345868024
2225	32-33	0.076779666027889
2225	34-35	-0.01830851839387293
2225	36-37	0.08247218177706372
2225	38-39	-0.014232698853504644
2225	40-41	0.06455687320770664
2225	42-43	0.22935095941740968
2225	44-45	0.1865205161609751
2225	46-47	0.18672905301416876
2225	48-49	0.16263059160407778
2225	50-51	0.08728408564537915
2225	52-53	0.08180882237121523
2225	54-55	0.059813312633018256
2225	56-57	0.167394861611875
2225	58-59	0.20873002777978655
2225	60-61	0.19010094765013008
2225	62-63	0.14111076067226236
2225	64-65	0.2220605027723508
2225	66-67	0.19778006212512622
2225	68-69	0.15156765047169785
2225	70-71	0.314418950518089
2225	72-73	0.329058926923274
2225	74-75	0.2373087126088862
2225	76-77	0.3247095766446364
2225	78-79	0.1473636770947806
2225	80-81	0.09919178111652371
2225	82-83	0.13126731196838648
2225	84-85	0.013858550386061097
2225	86-87	0.0904369696609848
2225	88-89	0.2207899255117951
2225	90-91	0.3537866939278942
2225	92-93	0.2236714756463698
2225	94-95	0.3583024832297639
2225	96-97	0.12339847498365941
2225	98-99	0.19083018905249105
2225	100	0.3953063275321469
2226	1	1.1693728833598929
2226	2	1.6240857155200477
2226	3	0.7524898378208604
2226	4	0.6999464290935951
2226	5	3.0985948019426637
2226	6	-0.4921111518155534
2226	7	0.08368418361854424
2226	8	0.15097116927459808
2226	9	0.16029765462874934
2226	10-11	0.004300574180071237
2226	12-13	0.07985157968378331
2226	14-15	0.016815896016105114
2226	16-17	0.056161426142402604
2226	18-19	0.036642347440029255
2226	20-21	0.14132495424918545
2226	22-23	0.10299097503477128
2226	24-25	0.14597588755140833
2226	26-27	0.08435349764824451
2226	28-29	0.13778660913380492
2226	30-31	0.045919147474627664
2226	32-33	0.05233406298079757
2226	34-35	0.0799085933103143
2226	36-37	0.005575631209083554
2226	38-39	0.1626841365742564
2226	40-41	-0.03993094246494877
2226	42-43	0.15479839983692756
2226	44-45	0.12891687896826198
2226	46-47	0.11086602443430849
2226	48-49	0.06758311497357994
2226	50-51	0.13774242289039762
2226	52-53	0.2353170972528602
2226	54-55	0.20219106701522094
2226	56-57	0.29110836562860953
2226	58-59	0.27898035300811586
2226	60-61	0.22718200662039578
2226	62-63	0.285611887236378
2226	64-65	0.32443953124492
2226	66-67	0.29742214331820804
2226	68-69	0.2699626654254885
2226	70-71	0.2655455146316683
2226	72-73	0.4247268520335794
2226	74-75	0.46396955645905535
2226	76-77	0.570925695899497
2226	78-79	0.4613991236299242
2226	80-81	0.28299675141010283
2226	82-83	0.18226043188040109
2226	84-85	0.25163398860609476
2226	86-87	0.2750754632838266
2226	88-89	0.21546808357947356
2226	90-91	0.22887420381885448
2226	92-93	0.0020855348982777855
2226	94-95	0.268459162816157
2226	96-97	0.23606863310497772
2226	98-99	0.21331184885695365
2226	100	0.3008157625065948
2227	1	1.5406872847736892
2227	2	1.7912454668048259
2227	3	0.6384659962873691
2227	4	-0.05424942059307192
2227	5	3.181644436567641
2227	6	0.28894437000937145
2227	7	0.43065493193261517
2227	8	0.2884150619822776
2227	9	0.20586924428115339
2227	10-11	0.1578346559720316
2227	12-13	0.14741072273298528
2227	14-15	0.21632678679390693
2227	16-17	0.12290640359106675
2227	18-19	0.05542101094805929
2227	20-21	0.07296824739487562
2227	22-23	0.1915923855775148
2227	24-25	0.2833720077456974
2227	26-27	0.22502254322795778
2227	28-29	0.13680744201538886
2227	30-31	0.14744827447768216
2227	32-33	0.06353044934591168
2227	34-35	0.21517798962837986
2227	36-37	0.18325549980417577
2227	38-39	0.20861418948316413
2227	40-41	0.26641961698167904
2227	42-43	0.35634521283430587
2227	44-45	0.1973698084736526
2227	46-47	0.2827491559259485
2227	48-49	0.21748716337221907
2227	50-51	0.35362200634118324
2227	52-53	0.3735405967216039
2227	54-55	0.32872554725027925
2227	56-57	0.40971159220449493
2227	58-59	0.5003974822663082
2227	60-61	0.40872433374487827
2227	62-63	0.37321346311945547
2227	64-65	0.3688212126205457
2227	66-67	0.3806649008696539
2227	68-69	0.36788716994660575
2227	70-71	0.5682020051882901
2227	72-73	0.4327648315205366
2227	74-75	0.3635002338665245
2227	76-77	0.7039033668234538
2227	78-79	0.4829018368376339
2227	80-81	0.3938117030548298
2227	82-83	0.3189054055343661
2227	84-85	0.32783318033582987
2227	86-87	0.3624581665169089
2227	88-89	0.40564707126296895
2227	90-91	0.5484143815190592
2227	92-93	0.5028522667975821
2227	94-95	0.6198856297372473
2227	96-97	0.48626649940157307
2227	98-99	0.7691159043028861
2227	100	0.5753850644993506
2228	1	1.2776379999682828
2228	2	1.8091184758873577
2228	3	-0.21308999072504697
2228	4	-0.0421146963598229
2228	5	3.2366826095306536
2228	6	0.11867430726530515
2228	7	0.3835804288392879
2228	8	0.2280485795988767
2228	9	0.134983258585045
2228	10-11	0.24535138406645984
2228	12-13	0.24393731217618608
2228	14-15	0.24824453941555902
2228	16-17	0.24367844628335433
2228	18-19	0.2095189827351689
2228	20-21	0.09175855936012312
2228	22-23	0.18831532445690158
2228	24-25	0.1901094534479526
2228	26-27	0.1750473611385388
2228	28-29	0.22319708046916986
2228	30-31	0.2183740236769296
2228	32-33	0.3189794204309919
2228	34-35	0.23643363878813517
2228	36-37	0.1841632045999333
2228	38-39	0.23866442861264403
2228	40-41	0.3012976938541243
2228	42-43	0.4066549568121829
2228	44-45	0.17473203479105592
2228	46-47	0.18100874852829207
2228	48-49	0.23379568427959185
2228	50-51	0.09288311818357897
2228	52-53	0.23682201811601544
2228	54-55	0.18060169902071266
2228	56-57	0.44021705488713536
2228	58-59	0.5033701469146337
2228	60-61	0.39155061385545054
2228	62-63	0.2819185166493625
2228	64-65	0.25512364559847356
2228	66-67	0.37861502372516753
2228	68-69	0.46562654623549093
2228	70-71	0.5365235191631044
2228	72-73	0.4264492902981303
2228	74-75	0.3188663871726547
2228	76-77	0.36323572389422054
2228	78-79	0.436372367968346
2228	80-81	0.3982796125366477
2228	82-83	0.33003644410222677
2228	84-85	0.3820164379813846
2228	86-87	0.3583590978055753
2228	88-89	0.3491109111559112
2228	90-91	0.3547572087315487
2228	92-93	0.4170529106920995
2228	94-95	0.6031541074258087
2228	96-97	0.44199463770177516
2228	98-99	0.5807184831717365
2228	100	0.6016399458121526
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	30.0
13	53.0
14	191.0
15	668.0
16	2467.0
17	6478.0
18	10590.0
19	13622.0
20	15863.0
21	18248.0
22	21196.0
23	23879.0
24	27167.0
25	30615.0
26	34803.0
27	39078.0
28	44730.0
29	50116.0
30	57851.0
31	66578.0
32	77826.0
33	93268.0
34	115209.0
35	148313.0
36	207983.0
37	344914.0
38	837786.0
39	1538709.0
40	1750.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.65185253256423	18.543351429810176	19.11543455107542	34.68936148655017
2	18.889577645434397	51.94693255570573	12.394954472438018	16.768535326421862
3	41.004537620421615	15.354645362470468	23.752023835105188	19.888793182002733
4	18.77787383279447	16.109662162814907	21.58543345254193	43.52703055184869
5	16.697315208613304	35.34359569929981	26.342036683732893	21.617052408353985
6	17.824814274535566	28.52507623405965	29.404401745073933	24.245707746330854
7	20.028689437362743	26.032583451458375	36.35848324051738	17.580243870661498
8	21.28248155800251	27.983167540517822	33.60557141145087	17.1287794900288
9	21.199321876531503	27.995256373334488	26.894363183524934	23.91105856660908
10-11	19.64341337463554	30.23405338042147	28.318586959047575	21.80394628589541
12-13	24.23291394918147	26.64695986742493	27.143528910456737	21.976597272936864
14-15	21.873463602038758	27.557904334251266	28.415467335216544	22.153164728493433
16-17	20.81745314141245	29.85074860684687	28.16028330166651	21.171514950074165
18-19	21.529441007670798	29.63220966370329	29.604650780251912	19.233698548374
20-21	22.782815371668946	28.97009149653745	29.867537201881678	18.379555929911923
22-23	21.792771295732276	30.308975423115676	28.691003950150147	19.2072493310019
24-25	22.354458155275445	28.14728062619632	26.43625386131158	23.062007357216654
26-27	20.037357887676205	26.571110927182147	30.040397067243934	23.35113411789771
28-29	19.363200613269154	28.102886553039895	30.703301282570134	21.830611551120818
30-31	20.012520816346782	30.16733372545205	28.22474016694857	21.595405291252597
32-33	20.797661920516056	29.979065170297194	28.786709385764574	20.43656352342218
34-35	21.253878282947095	29.066201633898448	30.195685043868366	19.484235039286098
36-37	21.90044807010792	28.11738491653092	28.63080782907278	21.35135918428838
38-39	21.574826525241047	29.23723326036462	29.01824879267416	20.169691421720167
40-41	20.827999251161444	29.779518221085443	29.89544720257362	19.49703532517949
42-43	20.701330765519156	29.806426985443252	29.432090436075843	20.060151812961752
44-45	20.617347710080026	30.133648182588896	28.647452820262032	20.601551287069046
46-47	19.892290065891785	28.21027928549869	28.80838639803066	23.089044250578862
48-49	20.939010002792443	28.02980328489923	30.1947881980078	20.83639851430053
50-51	21.992223982312183	30.724160250403333	28.225936368874937	19.05767939840955
52-53	20.421473060369728	30.4395426071715	28.185124798152028	20.953859534306744
54-55	22.2876036580119	28.589431031783473	28.018429773961586	21.10453553624304
56-57	21.356371429369734	28.810384847688013	29.517297242672164	20.31594648027009
58-59	21.228854279282807	28.072024714183495	28.868785547529885	21.83033545900381
60-61	19.60023979293838	30.34756660037374	29.665172399477697	20.387021207210186
62-63	20.561343989663467	31.192248852892533	27.142711097085577	21.103696060358423
64-65	19.866612741176446	30.303701641994625	28.811864617428412	21.01782099940052
66-67	20.246497306383503	30.274719378503445	29.439584687234742	20.03919862787831
68-69	20.964307655834325	29.892850643384396	29.210627937840943	19.932213762940336
70-71	19.409221612326537	31.018992000221413	29.326333472672577	20.24545291477947
72-73	20.19113149647479	30.739878344044	28.51595086241942	20.553039297061787
74-75	20.12040007509176	29.251502814243725	30.388584173132976	20.239512937531543
76-77	19.45501818416332	30.364798154351156	28.86345911376584	21.316724547719687
78-79	19.47072322290894	29.57992480902647	28.39909649682335	22.55025547124124
80-81	20.64908003956684	29.71513256811501	28.227668356538665	21.408119035779478
82-83	20.008271581503926	28.61119937670709	30.161481218836332	21.219047822952646
84-85	20.176392519962892	29.394205872039574	28.342895173631412	22.086506434366125
86-87	21.530433179694626	28.545872681874922	29.426020651277383	20.49767348715307
88-89	20.871435132445825	28.05221488043935	29.389036133599618	21.6873138535152
90-91	20.384866139022623	27.878924203540436	29.62906343399615	22.107146223440797
92-93	20.202867847125088	27.923937481674194	30.19291740611768	21.68027726508304
94-95	20.478521956114143	28.247228902702126	29.386869021021255	21.887380120162476
96-97	20.106470502072987	28.40105473108091	29.92653227261441	21.56594249423169
98-99	21.131984727861575	28.41759528310976	29.115875509565193	21.334544479463474
100	21.105091644057765	30.08202912755964	27.640424325864803	21.172454902517792
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	9.5
2	19.5
3	20.5
4	27.5
5	40.5
6	53.0
7	61.0
8	67.5
9	94.0
10	116.0
11	121.5
12	140.5
13	153.0
14	189.5
15	251.5
16	312.0
17	412.0
18	607.5
19	984.5
20	1626.0
21	2634.0
22	4040.5
23	6216.0
24	9578.5
25	14404.0
26	20671.0
27	27792.5
28	35871.5
29	45081.5
30	55776.0
31	68077.0
32	81584.0
33	96313.5
34	112888.5
35	132023.5
36	150606.5
37	163707.0
38	171997.5
39	178763.5
40	183334.0
41	184293.0
42	182138.5
43	179615.5
44	179201.5
45	175499.5
46	163143.0
47	148692.5
48	139070.0
49	132682.0
50	126260.0
51	116586.5
52	103265.0
53	89133.5
54	75640.0
55	62041.5
56	47640.0
57	35107.5
58	25216.0
59	17566.5
60	13240.0
61	10957.0
62	9022.5
63	6938.0
64	5121.0
65	4368.5
66	5090.0
67	5087.5
68	3512.5
69	3209.5
70	4359.0
71	4338.0
72	2524.5
73	891.0
74	354.0
75	223.5
76	152.5
77	112.5
78	90.5
79	83.0
80	73.0
81	53.0
82	37.5
83	34.5
84	27.0
85	23.5
86	25.0
87	22.0
88	21.0
89	17.5
90	17.0
91	15.5
92	13.0
93	16.5
94	18.5
95	22.0
96	23.0
97	24.0
98	55.0
99	127.5
100	132.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.353711676376463
2	0.005561385291467503
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	3.91646851511796E-4
30-31	0.009360359751131924
32-33	0.0
34-35	0.0
36-37	0.0
38-39	7.571839129228056E-4
40-41	0.00130548950503932
42-43	1.3054895050393202E-4
44-45	0.0
46-47	0.001697136356551116
48-49	1.30548950503932E-5
50-51	0.0
52-53	0.0014752031406944316
54-55	0.019386519149833904
56-57	0.011488307644346017
58-59	0.015691983850572626
60-61	2.6109790100786403E-4
62-63	0.011945228971109778
64-65	1.3054895050393202E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.697136356551116E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3829981.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.73594058212096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.04390645580463	58.608808668913234
2	9.570828889287359	13.348615094899799
3	2.802125574479336	5.862265876965974
4	1.2395686449783672	3.457699414946864
5	0.6523473924492927	2.274602949937271
6	0.3870937333626967	1.6196607353695431
7	0.2735066251652787	1.335126923293961
8	0.19391987125867008	1.081854769582973
9	0.1338789041710456	0.8402554175824352
>10	0.655251939017623	7.747167890056055
>50	0.03200246056279957	1.5357702567198872
>100	0.01454118291564669	1.7425816714425058
>500	8.759818819246148E-4	0.4173067556123127
>1k	1.5234466525155654E-4	0.12828357467712576
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.6109790100786402E-5	0.0
4	0.0	0.0	0.0	2.6109790100786402E-5	0.0
5	0.0	0.0	0.0	2.6109790100786402E-5	0.0
6	0.0	0.0	0.0	2.6109790100786402E-5	0.0
7	0.0	0.0	0.0	2.6109790100786402E-5	0.0
8	0.0	0.0	0.0	2.6109790100786402E-5	0.0
9	2.6109790100786402E-5	0.0	0.0	2.6109790100786402E-5	0.0
10-11	2.6109790100786402E-5	0.0	0.0	2.6109790100786402E-5	0.0
12-13	2.6109790100786402E-5	0.0	0.0	2.6109790100786402E-5	0.0
14-15	2.6109790100786402E-5	0.0	0.0	2.6109790100786402E-5	0.0
16-17	2.6109790100786402E-5	0.0	0.0	2.6109790100786402E-5	0.0
18-19	2.6109790100786402E-5	0.0	0.0	2.6109790100786402E-5	0.0
20-21	2.6109790100786402E-5	0.0	0.0	2.6109790100786402E-5	0.0
22-23	2.6109790100786402E-5	0.0	0.0	2.6109790100786402E-5	0.0
24-25	2.6109790100786402E-5	0.0	0.0	2.6109790100786402E-5	0.0
26-27	2.6109790100786402E-5	0.0	0.0	2.6109790100786402E-5	0.0
28-29	2.6109790100786402E-5	0.0	0.0	2.6109790100786402E-5	0.0
30-31	2.6109790100786402E-5	0.0	0.0	2.6109790100786402E-5	0.0
32-33	2.6109790100786402E-5	0.0	0.0	2.6109790100786402E-5	0.0
34-35	2.6109790100786402E-5	0.0	0.0	2.6109790100786402E-5	0.0
36-37	2.6109790100786402E-5	0.0	0.0	2.6109790100786402E-5	0.0
38-39	2.6109790100786402E-5	0.0	0.0	2.6109790100786402E-5	0.0
40-41	2.6109790100786402E-5	0.0	0.0	2.6109790100786402E-5	0.0
42-43	2.6109790100786402E-5	0.0	0.0	2.6109790100786402E-5	0.0
44-45	2.6109790100786402E-5	0.0	0.0	2.6109790100786402E-5	0.0
46-47	2.6109790100786402E-5	0.0	0.0	2.6109790100786402E-5	0.0
48-49	5.2219580201572804E-5	0.0	0.0	2.6109790100786402E-5	0.0
50-51	5.2219580201572804E-5	0.0	0.0	7.83293703023592E-5	0.0
52-53	1.30548950503932E-4	0.0	0.0	1.0443916040314561E-4	0.0
54-55	1.566587406047184E-4	0.0	0.0	1.0443916040314561E-4	0.0
56-57	1.566587406047184E-4	0.0	0.0	1.0443916040314561E-4	0.0
58-59	1.827685307055048E-4	0.0	0.0	1.0443916040314561E-4	0.0
60-61	2.0887832080629121E-4	0.0	0.0	1.0443916040314561E-4	0.0
62-63	2.2193321585668442E-4	0.0	0.0	1.0443916040314561E-4	0.0
64-65	3.002625861590436E-4	0.0	0.0	1.0443916040314561E-4	0.0
66-67	3.524821663606164E-4	0.0	0.0	1.0443916040314561E-4	0.0
68-69	4.5692132676376204E-4	0.0	0.0	1.0443916040314561E-4	0.0
70-71	5.352506970661212E-4	0.0	0.0	1.0443916040314561E-4	0.0
72-73	6.005251723180872E-4	0.0	0.0	1.0443916040314561E-4	0.0
74-75	6.396898574692667E-4	0.0	0.0	1.0443916040314561E-4	0.0
76-77	7.702388079731989E-4	0.0	0.0	1.0443916040314561E-4	0.0
78-79	9.268975485779173E-4	0.0	0.0	1.0443916040314561E-4	0.0
80-81	0.0013446541901904997	0.0	0.0	1.0443916040314561E-4	0.0
82-83	0.0015013129307952181	0.0	0.0	1.0443916040314561E-4	0.0
84-85	0.0015926971961479704	0.0	0.0	1.0443916040314561E-4	0.0
86-87	0.001697136356551116	0.0	0.0	1.0443916040314561E-4	1.3054895050393201E-5
88	0.001697136356551116	0.0	0.0	1.0443916040314561E-4	2.6109790100786402E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	16055	0.0	81.742584	8
CACCGGC	16440	0.0	78.08669	9
CTTCACC	17940	0.0	75.29911	6
CCTTCAC	19850	0.0	71.64793	5
GTGTGGC	5880	0.0	71.20476	7
TTCACCG	19755	0.0	68.16714	7
CGTAGTG	2955	0.0	66.39575	4
CGGGCCC	1120	0.0	64.53929	7
GGCGCGC	585	0.0	63.385914	6
CGGGGCA	1645	0.0	62.773785	94
ACCTTCA	7770	0.0	62.462738	4
AACGGGC	1180	0.0	60.85986	5
CAGCTGC	140	0.0	60.50483	1
TGTGGCA	6920	0.0	60.50347	8
GCCTTCA	5660	0.0	60.37212	4
GGGCCCG	1230	0.0	59.530704	8
GGCCCGA	1215	0.0	59.106697	9
CAGCGTA	1300	0.0	58.64314	1
ACGGGCC	1250	0.0	57.45171	6
AGGCGCG	645	0.0	56.76183	5
>>END_MODULE