FastQCFastQC Report
Tue 7 Mar 2017
1NF_2A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_2A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3452931
Sequences flagged as poor quality0
Sequence length96
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA188220.5451021175922716No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA78940.22861736883824207No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG77930.22569231762812522No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG75030.21729365573769066No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT65650.19012832865759555No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG63530.18398861720665718No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG62910.1821930412162884No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG60730.17587956434692728No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA59160.17133270256486446No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA53670.15543316677917976No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT53260.15424576975329077No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA52550.1521895456352878No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA51280.1485115109453389No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT50940.14752684024094315No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC50710.14686073947032247No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA49150.1423428385913301No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC43860.12702252086705468No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA41350.11975333419636824No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC39480.11433764532219148No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA38190.11060168882610164No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTTAAAAAAAAGCCATGAGTGCTC35130.10173965248653968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC1200.089.999913
GCTGCAC301.7942511E-889.999913
GCAGCCA355.0749804E-1089.99993
AGCAGCG750.089.99992
GCTGCTC256.3781226E-789.999893
GCAGCTC256.3781226E-789.999893
GCAGCCC202.2813423E-589.999893
GCAGCAA850.089.999893
TGCAGCC202.2813423E-589.999892
CAGCTGC5250.089.142751
AGCTGCC2200.087.9544452
AGCTGCT1500.086.99992
CAGCAGC4300.085.813861
CTGCTGC1850.085.135031
GCTGCCA800.084.37493
GCTGCTA750.083.999913
AGCAGCC750.083.999912
CAGCCCT202600.083.936241
CTGCAGC1150.082.173821
AGCCCTT173150.081.787382
Produced by FastQC (version 0.11.5)