##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1NF_2A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 3452931 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.817389342561436 12.0 12.0 12.0 2.0 32.0 2 14.561321092138824 12.0 12.0 12.0 12.0 22.0 3 13.720255921708253 12.0 12.0 12.0 12.0 22.0 4 13.750835739260356 12.0 12.0 12.0 12.0 22.0 5 22.429407653961228 22.0 22.0 22.0 22.0 27.0 6 26.78518452873805 27.0 22.0 32.0 22.0 32.0 7 34.937172796096995 37.0 37.0 37.0 27.0 41.0 8 37.56354384144948 41.0 37.0 41.0 27.0 41.0 9 38.505454930897834 41.0 37.0 41.0 32.0 41.0 10-11 38.863968321405785 41.0 41.0 41.0 37.0 41.0 12-13 39.04553725516091 41.0 41.0 41.0 37.0 41.0 14-15 38.998256698439675 41.0 41.0 41.0 37.0 41.0 16-17 38.90288772060606 41.0 41.0 41.0 37.0 41.0 18-19 38.83701658098583 41.0 41.0 41.0 37.0 41.0 20-21 38.858677164414814 41.0 41.0 41.0 37.0 41.0 22-23 38.84392969335327 41.0 41.0 41.0 34.5 41.0 24-25 38.8676092861398 41.0 41.0 41.0 37.0 41.0 26-27 38.83263812685513 41.0 41.0 41.0 37.0 41.0 28-29 38.77571691991528 41.0 41.0 41.0 34.5 41.0 30-31 38.658257868460154 41.0 41.0 41.0 32.0 41.0 32-33 38.7059149169213 41.0 41.0 41.0 32.0 41.0 34-35 38.676393909985464 41.0 41.0 41.0 32.0 41.0 36-37 38.59822698455312 41.0 41.0 41.0 32.0 41.0 38-39 38.50380140813703 41.0 41.0 41.0 32.0 41.0 40-41 38.54468290852033 41.0 41.0 41.0 32.0 41.0 42-43 38.29370279915816 41.0 41.0 41.0 32.0 41.0 44-45 38.40611874375711 41.0 41.0 41.0 32.0 41.0 46-47 38.408535096704796 41.0 41.0 41.0 32.0 41.0 48-49 38.35519939436959 41.0 41.0 41.0 32.0 41.0 50-51 38.23480414175667 41.0 41.0 41.0 32.0 41.0 52-53 38.21224229502414 41.0 41.0 41.0 32.0 41.0 54-55 38.08389785373643 41.0 37.0 41.0 32.0 41.0 56-57 37.86328165839399 41.0 37.0 41.0 27.0 41.0 58-59 37.86175672204281 41.0 37.0 41.0 29.5 41.0 60-61 37.87532533954487 41.0 37.0 41.0 29.5 41.0 62-63 37.8647673527215 41.0 37.0 41.0 29.5 41.0 64-65 37.86704136283059 41.0 37.0 41.0 29.5 41.0 66-67 37.72495439381789 41.0 37.0 41.0 27.0 41.0 68-69 37.73635832861994 41.0 37.0 41.0 27.0 41.0 70-71 37.71791964565756 41.0 37.0 41.0 27.0 41.0 72-73 37.54531280816211 41.0 37.0 41.0 27.0 41.0 74-75 37.49176453279837 41.0 37.0 41.0 27.0 41.0 76-77 36.41558244285797 39.0 34.5 41.0 24.5 41.0 78-79 36.930550016782846 41.0 37.0 41.0 27.0 41.0 80-81 37.59261595438774 41.0 37.0 41.0 27.0 41.0 82-83 37.78299986301493 41.0 37.0 41.0 27.0 41.0 84-85 37.69779804461774 41.0 37.0 41.0 27.0 41.0 86-87 37.55449341443544 41.0 37.0 41.0 27.0 41.0 88-89 37.460150376593106 41.0 37.0 41.0 27.0 41.0 90-91 37.31297555612898 41.0 37.0 41.0 27.0 41.0 92-93 37.20412759478831 41.0 37.0 41.0 27.0 41.0 94-95 37.05070199780998 41.0 37.0 41.0 27.0 41.0 96-97 36.99287083929566 41.0 37.0 41.0 27.0 41.0 98-99 36.85204801370198 41.0 37.0 41.0 27.0 41.0 100 36.74163659800905 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2672734679069126 1101 2 -0.49815493756915963 1101 3 -0.11750782807424365 1101 4 -0.12658132445348969 1101 5 -1.1046025903831023 1101 6 -0.8904178334561657 1101 7 -0.23047469118331776 1101 8 -0.26053795499237253 1101 9 -0.1372212928908212 1101 10-11 -0.0916594018150576 1101 12-13 -0.07088887709593195 1101 14-15 -0.09647531168671719 1101 16-17 -0.1833356389081544 1101 18-19 0.07429105216719023 1101 20-21 0.029026248774727037 1101 22-23 0.04993486578081985 1101 24-25 -0.09652122149379494 1101 26-27 0.06811563368840012 1101 28-29 -0.1551475211646789 1101 30-31 -0.20644129311925496 1101 32-33 -0.14572617190959392 1101 34-35 -0.15855438138127909 1101 36-37 -0.10685242659283745 1101 38-39 -0.05363220198444907 1101 40-41 -0.01838000529472339 1101 42-43 -0.033099685680767266 1101 44-45 -0.19473977111997698 1101 46-47 -0.128321001253191 1101 48-49 -0.06666284245241627 1101 50-51 -0.10471295592592611 1101 52-53 0.023274356408506947 1101 54-55 0.013364478349025433 1101 56-57 0.0601518962978318 1101 58-59 0.07359659367730842 1101 60-61 -0.004660618129577188 1101 62-63 -0.023785176102585126 1101 64-65 -0.03154007212701515 1101 66-67 -0.008065116406108075 1101 68-69 -0.04236620262879143 1101 70-71 -0.13723296430787002 1101 72-73 -0.04537991266420249 1101 74-75 0.04267983548187715 1101 76-77 -0.5216709418420251 1101 78-79 -0.08908868648340018 1101 80-81 -0.09862178402308075 1101 82-83 -0.32137062184567355 1101 84-85 -0.24370133508864456 1101 86-87 -0.06167614631040408 1101 88-89 -0.22689230100939284 1101 90-91 -0.16274740329532733 1101 92-93 0.07886930972000528 1101 94-95 0.007877796339961662 1101 96-97 -0.26317492370802853 1101 98-99 -0.41466206397260663 1101 100 -0.39338081135892367 1102 1 1.7758317299213964 1102 2 0.7855843646033218 1102 3 0.03390757679738243 1102 4 0.08902104552676171 1102 5 -0.7540430117129233 1102 6 -0.07244548322574929 1102 7 -0.1317255140931266 1102 8 -0.5093858812596537 1102 9 -0.33406131922393456 1102 10-11 -0.3946547935201252 1102 12-13 -0.2771100752526081 1102 14-15 -0.23093877449119304 1102 16-17 -0.3186220115217182 1102 18-19 -0.2160315284779628 1102 20-21 -0.0787749362154031 1102 22-23 -0.15842589787948214 1102 24-25 -0.2066265539493628 1102 26-27 -0.1731615223352989 1102 28-29 -0.4011975540810653 1102 30-31 -0.2732615696169489 1102 32-33 -0.30207903563573524 1102 34-35 -0.134196251032364 1102 36-37 -0.24970956944998335 1102 38-39 -0.2605117279884084 1102 40-41 -0.13194155894844073 1102 42-43 -0.13003846119097773 1102 44-45 -0.11689250318054434 1102 46-47 -0.37881474713864804 1102 48-49 -0.4203494783971138 1102 50-51 -0.11047332458952042 1102 52-53 -0.37139318802862675 1102 54-55 -0.26642485673985306 1102 56-57 -0.25847878177984995 1102 58-59 -0.3689643016485604 1102 60-61 -0.1590384983139046 1102 62-63 -0.38487800032904573 1102 64-65 -0.31165857113952455 1102 66-67 -0.29509605781493775 1102 68-69 -0.3687322329118743 1102 70-71 -0.4953633132216311 1102 72-73 -0.31644640377678 1102 74-75 -0.5164050888104157 1102 76-77 -0.1944820017498543 1102 78-79 -0.40212357785930664 1102 80-81 -0.3981609545299989 1102 82-83 -0.5058670010425175 1102 84-85 -0.4076249690583609 1102 86-87 -0.4171731838350894 1102 88-89 -0.5427250857361869 1102 90-91 -0.6273622815046735 1102 92-93 -0.4484512959416165 1102 94-95 -0.354202519657413 1102 96-97 -0.4256173200213951 1102 98-99 -0.5012321758883402 1102 100 -0.757436110898098 1103 1 1.5266157839553465 1103 2 0.7514845363458491 1103 3 0.29785152678666904 1103 4 1.0600029975742942 1103 5 2.8889018341795207 1103 6 0.044362303915651324 1103 7 -0.2868313294704663 1103 8 -0.11305908420781208 1103 9 -0.1625671370436379 1103 10-11 -0.24228065015119427 1103 12-13 -0.2678525203022204 1103 14-15 -0.4168313527050955 1103 16-17 -0.2900727307421178 1103 18-19 -0.25811999308645994 1103 20-21 -0.16826805707795245 1103 22-23 -0.07019486810152387 1103 24-25 -0.1723104100408932 1103 26-27 -0.08128848524579979 1103 28-29 -0.13752176444845077 1103 30-31 -0.13932865466779276 1103 32-33 -0.2899382711539076 1103 34-35 -0.3204758327665971 1103 36-37 -0.358550146424939 1103 38-39 -0.15066689575098735 1103 40-41 -0.2920961827301696 1103 42-43 -0.06157064431935311 1103 44-45 -0.5528217168395742 1103 46-47 -0.3953502663128603 1103 48-49 -0.05918266167314812 1103 50-51 -0.14023779198230812 1103 52-53 -0.16397600911331978 1103 54-55 -0.23598957214350236 1103 56-57 -0.19899342097067318 1103 58-59 -0.423017292044527 1103 60-61 -0.18103930429369797 1103 62-63 -0.20445637076224443 1103 64-65 -0.27578225684293045 1103 66-67 -0.3891900910110877 1103 68-69 -0.33037492186473116 1103 70-71 -0.44741184519493515 1103 72-73 -0.12767988590923096 1103 74-75 -0.30700865221804463 1103 76-77 -0.519416918326506 1103 78-79 -0.47081315019043757 1103 80-81 -0.23440712029761812 1103 82-83 -0.2247956474072197 1103 84-85 -0.4475359560283465 1103 86-87 -0.25643883188836014 1103 88-89 -0.23301097542796612 1103 90-91 -0.5320576688800998 1103 92-93 -0.15024659927754414 1103 94-95 -0.17105421382471064 1103 96-97 0.026533569165273718 1103 98-99 -0.15910974023072555 1103 100 -0.1693701626562074 1104 1 1.5044673341594006 1104 2 -0.6855373386731021 1104 3 0.05296443553408636 1104 4 0.0028697325619209124 1104 5 -1.078721417806296 1104 6 -0.5000542767985436 1104 7 -0.47203431052821543 1104 8 -0.31755261144918734 1104 9 -0.2310465693659154 1104 10-11 -0.2819918498455678 1104 12-13 -0.19763393549427377 1104 14-15 -0.27289625283944474 1104 16-17 -0.107950697894033 1104 18-19 -0.08536550256365416 1104 20-21 -0.028739383498702864 1104 22-23 -0.12199490856487927 1104 24-25 -0.3045889765355767 1104 26-27 -0.23109286642382898 1104 28-29 -0.08729907817782134 1104 30-31 -0.22500214626393245 1104 32-33 -0.22408526338703894 1104 34-35 -0.3527980048659529 1104 36-37 -0.31821634028410273 1104 38-39 -0.313219383723613 1104 40-41 -0.2764196646514918 1104 42-43 -0.2629570379373902 1104 44-45 -0.30606421526982075 1104 46-47 -0.3392589602560889 1104 48-49 -0.45580314462037563 1104 50-51 -0.40352600738848565 1104 52-53 -0.2083075285784517 1104 54-55 -0.35381599537586084 1104 56-57 -0.3666543248249994 1104 58-59 -0.3517072991945227 1104 60-61 -0.35540323303188615 1104 62-63 -0.23517356994835126 1104 64-65 -0.2569864771598347 1104 66-67 -0.5487255231834283 1104 68-69 -0.4803624906841364 1104 70-71 -0.4241953612840774 1104 72-73 -0.3232936913003428 1104 74-75 -0.30476850385976206 1104 76-77 -0.40084556142041805 1104 78-79 -0.3907851235287225 1104 80-81 -0.48669437483692946 1104 82-83 -0.4208267104743797 1104 84-85 -0.29669446708714275 1104 86-87 -0.22664903736392716 1104 88-89 -0.31606559489326713 1104 90-91 -0.3159329116464562 1104 92-93 -0.5077674635395297 1104 94-95 -0.33065524734920615 1104 96-97 -0.30981564392170213 1104 98-99 -0.3679795391873881 1104 100 -0.3412508872700002 1105 1 1.3313695332965345 1105 2 0.33280965602782153 1105 3 -0.11876117478801618 1105 4 -0.09564547655588207 1105 5 -1.0795891265306032 1105 6 0.5338973494490418 1105 7 0.057881461187704986 1105 8 0.007426503003614471 1105 9 0.0157156663360567 1105 10-11 0.005998657824569875 1105 12-13 -0.0692493669876697 1105 14-15 -0.013147646218214959 1105 16-17 0.0017428193128310454 1105 18-19 0.034676954626029044 1105 20-21 0.015664097022238366 1105 22-23 0.03269535977909044 1105 24-25 0.10055835233410448 1105 26-27 0.016224299276252907 1105 28-29 -0.08696107536080433 1105 30-31 0.01184197692649036 1105 32-33 -0.19697340160698218 1105 34-35 -0.008072463153382614 1105 36-37 -0.006338618604694091 1105 38-39 0.023936912814832567 1105 40-41 0.054522424871841224 1105 42-43 0.1570462714633294 1105 44-45 0.15040303982443248 1105 46-47 -0.10799037010114887 1105 48-49 -0.0425084344149127 1105 50-51 -0.08728149110582706 1105 52-53 -0.016785042814930762 1105 54-55 0.0011162224472798243 1105 56-57 0.13460486178887976 1105 58-59 -0.0034352503122505595 1105 60-61 0.0312104428647757 1105 62-63 -0.16530384733263048 1105 64-65 -0.08274249360997743 1105 66-67 -0.07508772498422189 1105 68-69 -0.15826901222147427 1105 70-71 -0.2441084693360125 1105 72-73 -0.11888976683538743 1105 74-75 -0.23219374974419793 1105 76-77 -0.1329415017543525 1105 78-79 -0.10210852058819597 1105 80-81 -0.3079191356116482 1105 82-83 -0.24649898294298822 1105 84-85 -0.24849324745190415 1105 86-87 0.012303819819287298 1105 88-89 -0.006151316443521182 1105 90-91 -0.1685455220775225 1105 92-93 0.016678716322367393 1105 94-95 0.11639131430451499 1105 96-97 -0.02661065076247837 1105 98-99 -0.19503005597856315 1105 100 -0.3720525290556296 1106 1 1.3141928528270075 1106 2 -0.5074347199790381 1106 3 -0.009482114766715455 1106 4 -0.23719208908764955 1106 5 -1.0790604654081513 1106 6 -0.7088253898998786 1106 7 -0.2366692922024498 1106 8 -0.1274743586178957 1106 9 -0.03121406210514266 1106 10-11 -0.15332634765427855 1106 12-13 -0.14598597071482544 1106 14-15 -0.12726155250486215 1106 16-17 -0.019934209111838186 1106 18-19 0.006015832172728608 1106 20-21 -0.029015457441389003 1106 22-23 -0.0720593222360506 1106 24-25 -0.14719147368833063 1106 26-27 -0.2036372107337101 1106 28-29 -0.14810025357149925 1106 30-31 0.1282521948886597 1106 32-33 0.05321403279097581 1106 34-35 -0.10747997821541588 1106 36-37 -0.37970313333223515 1106 38-39 -0.17651985039496765 1106 40-41 -0.23917574230811312 1106 42-43 -0.0028804464930800577 1106 44-45 -0.06780520267530932 1106 46-47 -0.4406787368650953 1106 48-49 -0.15187496382193189 1106 50-51 -0.12775194183269178 1106 52-53 -0.28804394835184155 1106 54-55 -0.23628049639343374 1106 56-57 -0.23824829345293352 1106 58-59 -0.3099702707474066 1106 60-61 -0.1389501932202819 1106 62-63 -0.21555356762284106 1106 64-65 -0.44602399892927025 1106 66-67 -0.4835514934344971 1106 68-69 -0.43875834095641864 1106 70-71 -0.4885959756318812 1106 72-73 -0.34042472959842485 1106 74-75 -0.5109875180791335 1106 76-77 -0.40155229566878603 1106 78-79 -0.30799319172253803 1106 80-81 -0.5138163023725113 1106 82-83 -0.37375540473382785 1106 84-85 -0.6090612794099428 1106 86-87 -0.4947888900399562 1106 88-89 -0.41607899560197126 1106 90-91 -0.6355234259650047 1106 92-93 -0.49517482724508 1106 94-95 -0.5364350041763188 1106 96-97 -0.3170820626913766 1106 98-99 -0.5633626780223864 1106 100 -0.5000327359832042 1107 1 1.2705400798036042 1107 2 -0.5058426834219656 1107 3 -0.20320905604610395 1107 4 -0.21218353121195932 1107 5 -1.239733902305364 1107 6 -0.3870504593137589 1107 7 -0.7103943860674065 1107 8 -0.5828629948456907 1107 9 -0.46946608203548124 1107 10-11 -0.38321468775240675 1107 12-13 -0.446319483356028 1107 14-15 -0.32610566876157066 1107 16-17 -0.3049780308689449 1107 18-19 -0.33826995197319576 1107 20-21 -0.3555756260994656 1107 22-23 -0.26982693923444145 1107 24-25 -0.27645462700742485 1107 26-27 -0.44231924354161833 1107 28-29 -0.39156828515142195 1107 30-31 -0.39435771268627917 1107 32-33 -0.34075008390041006 1107 34-35 -0.3684955671331025 1107 36-37 -0.4208892820708314 1107 38-39 -0.30439956198097917 1107 40-41 -0.46481899571360685 1107 42-43 -0.33607902724411076 1107 44-45 -0.21546327886485273 1107 46-47 -0.3559657109632539 1107 48-49 -0.4658530447930218 1107 50-51 -0.6204485148789161 1107 52-53 -0.45313938904168793 1107 54-55 -0.5096553422158294 1107 56-57 -0.39831637610843273 1107 58-59 -0.6581773550384042 1107 60-61 -0.4055188382504653 1107 62-63 -0.5793494065935008 1107 64-65 -0.47222756226569373 1107 66-67 -0.41516559029334843 1107 68-69 -0.5755295495458057 1107 70-71 -0.44512539027319065 1107 72-73 -0.37840947212861664 1107 74-75 -0.4553921344417944 1107 76-77 -0.555655403128533 1107 78-79 -0.7827831026662935 1107 80-81 -0.6200860466142828 1107 82-83 -0.4983864380361922 1107 84-85 -0.37617866086299045 1107 86-87 -0.3351987443658899 1107 88-89 -0.2744065965169824 1107 90-91 -0.19506966837838036 1107 92-93 -0.38772991007988367 1107 94-95 -0.4055193929809846 1107 96-97 -0.46046321145714586 1107 98-99 -0.5510245033765173 1107 100 -0.6453926286158236 1108 1 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0.20233031527575918 2223 24-25 0.1210144280017289 2223 26-27 0.18692295810699733 2223 28-29 0.07437058880883995 2223 30-31 0.11560985984813499 2223 32-33 0.08470868193268899 2223 34-35 0.16841933614543336 2223 36-37 0.06439324289083714 2223 38-39 0.08290975971338099 2223 40-41 0.11114525394048513 2223 42-43 0.22610424403006846 2223 44-45 0.29342745606866316 2223 46-47 0.24088027727310646 2223 48-49 0.2148452943010426 2223 50-51 0.27079307775305494 2223 52-53 0.18619485959737148 2223 54-55 0.1489882067485766 2223 56-57 0.2185981532125112 2223 58-59 0.38990376170580987 2223 60-61 0.29992101601291665 2223 62-63 0.3268831309224609 2223 64-65 0.08673999424195955 2223 66-67 0.1827836796880078 2223 68-69 0.37164530768253456 2223 70-71 0.3350204531881644 2223 72-73 0.4389870351074592 2223 74-75 0.32303366435834135 2223 76-77 0.363788949310468 2223 78-79 0.5208967468959074 2223 80-81 0.3096509543526622 2223 82-83 0.24635299365511543 2223 84-85 0.36491233370394127 2223 86-87 0.44433370179757503 2223 88-89 0.4214325376109187 2223 90-91 0.48234462855462823 2223 92-93 0.3051486552050946 2223 94-95 0.5325405150254454 2223 96-97 0.43550082450689587 2223 98-99 0.5687374993266658 2223 100 0.5894594711560615 2224 1 1.3521349704716492 2224 2 -0.81714821028147 2224 3 -0.008223691032331004 2224 4 -0.31540376623262034 2224 5 -0.9408842552667949 2224 6 0.5299714285994632 2224 7 0.3964218587673045 2224 8 0.25887135863226973 2224 9 0.19840011545074532 2224 10-11 0.2445150062042032 2224 12-13 0.20286257156821108 2224 14-15 0.32717708778113774 2224 16-17 0.3384972941138358 2224 18-19 0.18956046512391111 2224 20-21 0.21057429642119985 2224 22-23 0.2619616497807087 2224 24-25 0.2733743803961488 2224 26-27 0.24268812775658688 2224 28-29 0.16263281677694863 2224 30-31 0.12092508690049897 2224 32-33 0.07766148204311207 2224 34-35 0.11256568458865246 2224 36-37 0.1509272855944559 2224 38-39 0.17423045265400816 2224 40-41 0.18527710018860688 2224 42-43 0.33795463322420716 2224 44-45 0.2955424998025009 2224 46-47 0.35096481589624773 2224 48-49 0.07645896020009246 2224 50-51 0.24816169948059041 2224 52-53 0.21996790908158914 2224 54-55 0.30272967629311864 2224 56-57 0.5280120179170424 2224 58-59 0.47744151118060074 2224 60-61 0.33165919205256245 2224 62-63 0.3240158331620151 2224 64-65 0.45427994892576606 2224 66-67 0.38975348684163436 2224 68-69 0.3817836929645253 2224 70-71 0.4028245279430678 2224 72-73 0.45715139219644385 2224 74-75 0.43080588983507084 2224 76-77 0.5964631187378515 2224 78-79 0.3682798708185544 2224 80-81 0.47748997998695586 2224 82-83 0.3834590229658801 2224 84-85 0.17008053980849525 2224 86-87 0.45077625778631614 2224 88-89 0.4622189092509217 2224 90-91 0.5392969918316695 2224 92-93 0.34496330402135555 2224 94-95 0.5957534203799 2224 96-97 0.5425292127852757 2224 98-99 0.4968933470022634 2224 100 0.6830518660467106 2225 1 1.3196207383980045 2225 2 1.6176971547056098 2225 3 0.8813313800271008 2225 4 0.8879760989159582 2225 5 3.213594305779715 2225 6 -0.12269784223386893 2225 7 0.17836327511097494 2225 8 0.3196630051115932 2225 9 0.20834490307994002 2225 10-11 0.13966575515446067 2225 12-13 0.17742904847390406 2225 14-15 0.20199035489999773 2225 16-17 0.14982769018941866 2225 18-19 -0.04087421874236696 2225 20-21 0.15881900733851495 2225 22-23 0.19599699781043256 2225 24-25 0.14023131272819 2225 26-27 0.17510975041486176 2225 28-29 0.14325297181571273 2225 30-31 0.23364109142246292 2225 32-33 0.17995324964647352 2225 34-35 0.18772819266359164 2225 36-37 0.12807269499368346 2225 38-39 0.13905149189819355 2225 40-41 0.09553176346379644 2225 42-43 0.32217822818545017 2225 44-45 0.10563389797756173 2225 46-47 0.26079806848022713 2225 48-49 0.15858862347528202 2225 50-51 0.34232301650255437 2225 52-53 0.24664673861970954 2225 54-55 0.20245033399660883 2225 56-57 0.3383708983272484 2225 58-59 0.30688121160049775 2225 60-61 0.28639917913267254 2225 62-63 0.18512568256446826 2225 64-65 0.3550162820976013 2225 66-67 0.3982725436891812 2225 68-69 0.4421425733747313 2225 70-71 0.318509585275109 2225 72-73 0.3118933980104046 2225 74-75 0.35252696137899875 2225 76-77 0.475684795901941 2225 78-79 0.2617159577048582 2225 80-81 0.3610632903067241 2225 82-83 0.26345160411898405 2225 84-85 0.33675351501844375 2225 86-87 0.297038319721068 2225 88-89 0.2916523011275345 2225 90-91 0.5058806380687528 2225 92-93 0.5598083157300167 2225 94-95 0.5478185851085797 2225 96-97 0.5419639673072467 2225 98-99 0.4512018297219029 2225 100 0.5026754731203553 2226 1 1.5041730787731762 2226 2 1.1977512012961373 2226 3 0.7828664807233956 2226 4 1.0211077437864642 2226 5 3.1706923589189557 2226 6 -0.23497519592343608 2226 7 0.2207449742253189 2226 8 0.16810571375313543 2226 9 0.20774082394311222 2226 10-11 0.08585093376463959 2226 12-13 0.12565946644735249 2226 14-15 0.11701984382438724 2226 16-17 0.03939955834729858 2226 18-19 0.015808129325563414 2226 20-21 0.10348042714639405 2226 22-23 0.11941085859114509 2226 24-25 0.08964893112770511 2226 26-27 0.14021739978267078 2226 28-29 0.14672950888891023 2226 30-31 0.14745379237123757 2226 32-33 0.06957812883064918 2226 34-35 0.15299487511028786 2226 36-37 0.1430476935142977 2226 38-39 0.14998039301661237 2226 40-41 0.17015156345195948 2226 42-43 0.26144978271530306 2226 44-45 0.16692573970390612 2226 46-47 0.26287042976393593 2226 48-49 0.29841198580442096 2226 50-51 0.16071587012308441 2226 52-53 0.25154964214456754 2226 54-55 0.31596296788027445 2226 56-57 0.39779995724592965 2226 58-59 0.33107889588161754 2226 60-61 0.33487961189989335 2226 62-63 0.3357805536613725 2226 64-65 0.30527837847410666 2226 66-67 0.09478688378820266 2226 68-69 0.2832192149906021 2226 70-71 0.4994793665244899 2226 72-73 0.25481161802341745 2226 74-75 0.34936355920530104 2226 76-77 0.4631014576373431 2226 78-79 0.3708989468363697 2226 80-81 0.23357423770006136 2226 82-83 0.17444855660137648 2226 84-85 0.29016915105213315 2226 86-87 0.42567512268770713 2226 88-89 0.3000933824538521 2226 90-91 0.40908990094426656 2226 92-93 0.22600034878916375 2226 94-95 0.4927894413722598 2226 96-97 0.39089117207281276 2226 98-99 0.4551049206345823 2226 100 0.40940812002254034 2227 1 1.429811865927542 2227 2 1.554890824416038 2227 3 0.6137727974287941 2227 4 -0.02817717845762857 2227 5 3.2502222754541137 2227 6 0.03496418731428008 2227 7 0.3966095255822495 2227 8 0.30459560931300445 2227 9 0.27940130421786336 2227 10-11 0.23883113442929726 2227 12-13 0.0859289799244678 2227 14-15 0.19990591913290245 2227 16-17 0.27529901980603455 2227 18-19 0.27586492641272997 2227 20-21 0.18424849672045696 2227 22-23 0.19930926264198234 2227 24-25 0.25430682118527415 2227 26-27 0.17013568425462466 2227 28-29 0.08217072252482183 2227 30-31 0.077066657185199 2227 32-33 0.019336384144644114 2227 34-35 0.14612223822555848 2227 36-37 0.14156005765446622 2227 38-39 0.17762920187890785 2227 40-41 0.1951015948572632 2227 42-43 0.42800732315734535 2227 44-45 0.3277572146500418 2227 46-47 0.19494102707385963 2227 48-49 0.1655913244902365 2227 50-51 0.18750772170236019 2227 52-53 0.29918789544994695 2227 54-55 0.28849706506630923 2227 56-57 0.5684122495202217 2227 58-59 0.3804591556524528 2227 60-61 0.29071539015458114 2227 62-63 0.3658904392933877 2227 64-65 0.379808554319105 2227 66-67 0.4255108603992781 2227 68-69 0.24413340154386987 2227 70-71 0.4638384637311077 2227 72-73 0.4088909013898032 2227 74-75 0.37302820613642496 2227 76-77 0.44332815617854493 2227 78-79 0.4967574990807151 2227 80-81 0.3229932745821529 2227 82-83 0.2670886950481304 2227 84-85 0.2878400444692062 2227 86-87 0.4658606362405635 2227 88-89 0.40467401875903874 2227 90-91 0.38138984751820715 2227 92-93 0.27202362658080403 2227 94-95 0.45838931558640894 2227 96-97 0.35036176427886545 2227 98-99 0.5041421093701643 2227 100 0.5862323235914673 2228 1 1.5552133781525637 2228 2 1.5063700914316271 2228 3 -0.12253434985397682 2228 4 -0.14184112364136148 2228 5 3.127660238209028 2228 6 0.1478753245928992 2228 7 0.3326266548367869 2228 8 0.4906831556823761 2228 9 0.30772664693088814 2228 10-11 0.2875655272876969 2228 12-13 0.23239678596542745 2228 14-15 0.21937496942538814 2228 16-17 0.18607740163265163 2228 18-19 0.19997760084077498 2228 20-21 0.18794043575037023 2228 22-23 0.275796441547449 2228 24-25 0.3127961728433206 2228 26-27 0.19218439912025786 2228 28-29 0.24352458751022255 2228 30-31 0.2642355556004645 2228 32-33 0.33750059157321033 2228 34-35 0.3072484065306966 2228 36-37 0.27138906863560663 2228 38-39 0.1816336281863542 2228 40-41 0.3301907824452499 2228 42-43 0.5231594142840876 2228 44-45 0.31890667162246444 2228 46-47 0.26142125801920457 2228 48-49 0.36070681632801893 2228 50-51 0.32822887997610906 2228 52-53 0.23608463003299818 2228 54-55 0.05869740188750683 2228 56-57 0.4651041005999659 2228 58-59 0.48122674623103023 2228 60-61 0.2953509547807158 2228 62-63 0.42517792064800375 2228 64-65 0.48033881409947554 2228 66-67 0.4779249165566526 2228 68-69 0.49012537539127976 2228 70-71 0.5249961552845619 2228 72-73 0.5892471835028843 2228 74-75 0.40052744913302263 2228 76-77 0.5630295337434603 2228 78-79 0.6094673558089525 2228 80-81 0.6087231879900799 2228 82-83 0.4564365098074603 2228 84-85 0.4127491289555536 2228 86-87 0.49336210647948064 2228 88-89 0.5458019336365325 2228 90-91 0.5474514294217272 2228 92-93 0.47707676288502654 2228 94-95 0.7853785304503376 2228 96-97 0.49749038793738265 2228 98-99 0.6966877779380667 2228 100 0.8338055358954151 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 33.0 13 38.0 14 161.0 15 633.0 16 2372.0 17 5902.0 18 9610.0 19 12348.0 20 14239.0 21 16783.0 22 18840.0 23 21685.0 24 24385.0 25 27876.0 26 31331.0 27 35319.0 28 39912.0 29 44966.0 30 51854.0 31 59852.0 32 69934.0 33 83192.0 34 102638.0 35 132524.0 36 185873.0 37 305732.0 38 750570.0 39 1402777.0 40 1552.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.624425035020593 18.428313627715404 20.676328691797863 35.27093264546614 2 20.035421246376057 50.83875657812803 12.528752610253335 16.597069565242574 3 40.2946076825746 15.404101616858256 23.631981061886265 20.66930963868088 4 17.63785607068314 17.0896840973654 21.90307307038571 43.36938676156576 5 16.659759491284362 34.91306371311793 26.668329022502913 21.7588477730948 6 17.5704350883351 28.907672930620393 29.86836400727382 23.65352797377069 7 20.116069507325804 26.027540081165828 36.296583974600125 17.559806436908236 8 21.028135227723922 28.223384712871468 33.42560277051583 17.322877288888776 9 21.172418446820977 28.12975411324466 26.967929564766862 23.729897875167502 10-11 19.551708389191674 30.389587860284493 28.528183737236567 21.530520013287262 12-13 24.069869916311678 26.554787802015156 27.360653890853886 22.014688390819277 14-15 22.034396285358728 27.538213187578904 28.403463608163616 22.023926918898756 16-17 20.709739059367244 29.991665052096323 28.256849036369392 21.041746852167044 18-19 21.395735970397322 29.570819689127877 29.746568929410984 19.286875411063818 20-21 22.589533355864916 29.083016718260517 29.924113745684462 18.403336180190106 22-23 21.705588672348217 30.411178213523527 28.63567212898259 19.24756098514566 24-25 22.253919930632847 28.44305316266094 26.38804540258696 22.914981504119254 26-27 20.09937354670568 26.64020798562149 29.97294182826127 23.28747663941156 28-29 19.31431338446013 28.260846585287197 30.575294493629084 21.849545536623584 30-31 19.990604208208314 30.109081261579103 28.30440280025876 21.59591172995382 32-33 20.599137370541143 30.192827484823763 28.63510739137272 20.572927753262373 34-35 21.300975895550767 29.119970830578428 30.23597923039875 19.343074043472054 36-37 21.903941897477825 28.21469644195033 28.644968578868212 21.236393081703632 38-39 21.51618305882503 29.286562226950615 29.048762185767853 20.148492528456508 40-41 20.911092138210503 29.755844255140207 29.875440140080244 19.45762346656905 42-43 20.50452840379649 30.000962951003167 29.239884127309363 20.254624517890978 44-45 20.612213218277457 30.13403685159072 28.764968080740683 20.48878184939114 46-47 19.861966521646707 28.21752299109897 28.85592167416094 23.064588813093383 48-49 20.846318678247552 28.062970270764172 30.362712142235104 20.72799890875317 50-51 21.986205342649477 30.77922785019452 28.07529313502065 19.159273672135356 52-53 20.386450582722603 30.407298357475693 28.175513443607255 21.03073761619445 54-55 22.231142308723406 28.64109104228435 28.022988429168905 21.10477821982334 56-57 21.354994107235285 28.905075366273923 29.426879754290976 20.313050772199823 58-59 21.286722126130524 28.206613223173292 28.90575815914088 21.6009064915553 60-61 19.559112925997525 30.42697133589638 29.640594568373192 20.373321169732908 62-63 20.52336058255512 31.23679857101252 27.315482672122016 20.92435817431035 64-65 19.791316269653425 30.236942351977458 28.869074468237617 21.1026669101315 66-67 20.043450042876618 30.40626934045308 29.512260743119395 20.038019873550905 68-69 20.963378648458367 29.82250441726174 29.22465001472662 19.989466919553273 70-71 19.40105377141912 31.030231997106227 29.38536854631616 20.183345685158493 72-73 20.14051250951728 30.644834779496026 28.669469502865823 20.545183208120868 74-75 19.939292154983693 29.33024146732153 30.383969445088827 20.34649693260595 76-77 19.64047355710265 30.46996884675657 28.745216744846623 21.14434085129416 78-79 19.511105203086885 29.530303385732292 28.442343620535716 22.516247790645107 80-81 20.659872331447637 29.750511558966448 28.39929503257455 21.19032107701136 82-83 19.87091546283433 28.602019559614718 30.060736805919376 21.46632817163158 84-85 20.30793549016763 29.654444296743836 28.100995936495693 21.93662427659284 86-87 21.375535740505676 28.70141337895255 29.26745422946476 20.655596651077012 88-89 21.498083801848342 28.139108484936422 29.61470993773116 20.748097775484077 90-91 21.53118611405788 28.452856428350294 29.00024645728513 21.0157110003067 92-93 21.110224907477154 29.187478695635676 29.24912197782116 20.453174419066006 94-95 21.405785403762774 29.28089208848946 29.04386157730925 20.26946093043852 96-97 20.508388380769844 29.269061559585175 29.4125917952024 20.809958264442585 98-99 22.37364430392614 29.185422471517676 28.250376274533146 20.19055695002304 100 21.83327729398589 31.280179071055862 27.441932665321144 19.4446109696371 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5.0 1 10.0 2 17.5 3 25.5 4 38.0 5 39.5 6 38.0 7 58.0 8 72.5 9 80.5 10 96.0 11 127.0 12 150.5 13 161.5 14 208.0 15 274.0 16 335.0 17 426.5 18 626.0 19 986.0 20 1530.5 21 2361.5 22 3647.0 23 5655.5 24 8776.0 25 13197.0 26 18987.0 27 25368.5 28 32328.0 29 40744.5 30 50922.5 31 62227.5 32 74719.5 33 88744.0 34 103717.5 35 120458.5 36 137222.0 37 149531.0 38 156989.0 39 162416.5 40 166245.5 41 167646.5 42 166354.0 43 163933.0 44 162464.0 45 157802.5 46 145762.5 47 132198.0 48 124046.0 49 118862.0 50 112503.5 51 103588.5 52 91350.5 53 77848.5 54 65406.0 55 53630.0 56 41750.0 57 30889.5 58 22049.0 59 15647.0 60 12013.5 61 10076.0 62 8273.5 63 6198.5 64 4485.5 65 3767.0 66 4073.0 67 3900.5 68 2851.0 69 2689.5 70 3501.0 71 3383.0 72 1952.5 73 761.5 74 330.5 75 192.5 76 130.0 77 98.0 78 82.0 79 74.5 80 60.5 81 48.5 82 37.5 83 35.0 84 33.5 85 27.5 86 21.5 87 17.5 88 18.0 89 18.0 90 21.0 91 21.0 92 15.0 93 14.5 94 18.5 95 18.0 96 19.0 97 27.0 98 52.0 99 109.0 100 122.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.349053890738043 2 0.005821141517163245 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.620112883807988E-4 30-31 0.009310930337154145 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.688270921139172E-4 40-41 0.001534927862734587 42-43 1.0136316074662367E-4 44-45 0.0 46-47 0.0021141459241438647 48-49 0.0 50-51 0.0 52-53 0.001288760186635644 54-55 0.019476207314886976 56-57 0.01135267400362185 58-59 0.014465971083696719 60-61 1.7376541842278341E-4 62-63 0.012062216128848216 64-65 7.240225767615976E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.5928496688755148E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3452931.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.05461912367068 #Duplication Level Percentage of deduplicated Percentage of total 1 85.0670671441599 61.29475123040162 2 9.140930452827892 13.172925244289523 3 2.595181497348527 5.609844430447366 4 1.1456424888874999 3.301953327547316 5 0.5651139418880973 2.03595349221115 6 0.36250251028081715 1.5671988185795276 7 0.245746410151615 1.239501478913878 8 0.17193687753704012 0.9911076979395708 9 0.11165141795117328 0.7240500355580602 >10 0.5538435314447012 6.844423157928289 >50 0.02820712712573073 1.3809940404151821 >100 0.011358637366675908 1.413002280084898 >500 7.361667286327106E-4 0.34649658749564327 >1k 8.179630188259141E-5 0.07779817818794882 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 1.4480451535231953E-5 0.0 0.0 0.0 0.0 20-21 2.8960903070463906E-5 0.0 0.0 0.0 0.0 22-23 2.8960903070463906E-5 1.4480451535231953E-5 0.0 0.0 0.0 24-25 2.8960903070463906E-5 2.8960903070463906E-5 0.0 0.0 0.0 26-27 2.8960903070463906E-5 2.8960903070463906E-5 0.0 0.0 0.0 28-29 2.8960903070463906E-5 2.8960903070463906E-5 0.0 0.0 0.0 30-31 2.8960903070463906E-5 2.8960903070463906E-5 0.0 0.0 0.0 32-33 2.8960903070463906E-5 2.8960903070463906E-5 0.0 0.0 0.0 34-35 2.8960903070463906E-5 2.8960903070463906E-5 0.0 0.0 0.0 36-37 2.8960903070463906E-5 2.8960903070463906E-5 0.0 0.0 0.0 38-39 2.8960903070463906E-5 2.8960903070463906E-5 0.0 1.4480451535231953E-5 0.0 40-41 2.8960903070463906E-5 2.8960903070463906E-5 0.0 2.8960903070463906E-5 0.0 42-43 2.8960903070463906E-5 2.8960903070463906E-5 0.0 2.8960903070463906E-5 0.0 44-45 2.8960903070463906E-5 2.8960903070463906E-5 0.0 2.8960903070463906E-5 0.0 46-47 2.8960903070463906E-5 2.8960903070463906E-5 0.0 2.8960903070463906E-5 0.0 48-49 2.8960903070463906E-5 2.8960903070463906E-5 0.0 2.8960903070463906E-5 0.0 50-51 2.8960903070463906E-5 2.8960903070463906E-5 0.0 2.8960903070463906E-5 0.0 52-53 2.8960903070463906E-5 2.8960903070463906E-5 0.0 5.792180614092781E-5 0.0 54-55 5.7921806140927805E-5 2.8960903070463906E-5 0.0 5.792180614092781E-5 0.0 56-57 1.1584361228185562E-4 2.8960903070463906E-5 1.5928496688755148E-4 5.792180614092781E-5 0.0 58-59 1.1584361228185562E-4 2.8960903070463906E-5 3.1856993377510297E-4 5.792180614092781E-5 0.0 60-61 1.7376541842278341E-4 2.8960903070463906E-5 3.1856993377510297E-4 5.792180614092781E-5 0.0 62-63 1.8824586995801537E-4 2.8960903070463906E-5 3.1856993377510297E-4 5.792180614092781E-5 0.0 64-65 2.6064812763417514E-4 2.8960903070463906E-5 3.1856993377510297E-4 5.792180614092781E-5 0.0 66-67 2.6064812763417514E-4 5.792180614092781E-5 3.1856993377510297E-4 5.792180614092781E-5 0.0 68-69 3.1856993377510297E-4 5.792180614092781E-5 3.1856993377510297E-4 5.792180614092781E-5 0.0 70-71 4.344135460569586E-4 5.792180614092781E-5 3.1856993377510297E-4 5.792180614092781E-5 0.0 72-73 5.068158037331183E-4 5.792180614092781E-5 3.1856993377510297E-4 5.792180614092781E-5 0.0 74-75 5.647376098740461E-4 5.792180614092781E-5 3.1856993377510297E-4 5.792180614092781E-5 0.0 76-77 5.792180614092781E-4 5.792180614092781E-5 3.1856993377510297E-4 5.792180614092781E-5 0.0 78-79 7.095421252263656E-4 5.792180614092781E-5 3.1856993377510297E-4 5.792180614092781E-5 0.0 80-81 8.109052859729893E-4 5.792180614092781E-5 3.1856993377510297E-4 5.792180614092781E-5 0.0 82-83 0.0010715534136071644 5.792180614092781E-5 3.1856993377510297E-4 5.792180614092781E-5 0.0 84-85 0.0013466819927765716 5.792180614092781E-5 3.1856993377510297E-4 5.792180614092781E-5 0.0 86-87 0.0014190842504527314 8.688270921139171E-5 3.1856993377510297E-4 5.792180614092781E-5 0.0 88 0.0014190842504527314 8.688270921139171E-5 3.1856993377510297E-4 5.792180614092781E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 13400 0.0 81.64945 8 CACCGGC 13540 0.0 78.10132 9 CTTCACC 15195 0.0 74.01197 6 CCTTCAC 16615 0.0 71.98051 5 CCGCAGC 45 9.672658E-8 70.58714 1 GTGTGGC 5565 0.0 69.66757 7 CGGGGCA 1425 0.0 67.52348 94 GGGCCCG 1110 0.0 66.38844 8 TTCACCG 17015 0.0 66.316 7 CGCTGCT 80 0.0 66.17544 1 CGGGCCC 1130 0.0 65.213425 7 CGTAGTG 2565 0.0 65.1446 4 AACGGGC 1115 0.0 64.4069 5 CAGCTGC 125 0.0 63.52842 1 GGCCCGA 1180 0.0 62.05238 9 ACCTTCA 6685 0.0 61.997635 4 ATGCAGC 70 2.1827873E-11 60.350258 2 GGCTGCC 80 2.3646862E-11 59.557896 1 TGTGGCA 6575 0.0 59.465496 8 ACGGGCC 1225 0.0 58.623425 6 >>END_MODULE