FastQCFastQC Report
Tue 7 Mar 2017
1NF_2A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_2A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3452931
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG134000.081.649458
CACCGGC135400.078.101329
CTTCACC151950.074.011976
CCTTCAC166150.071.980515
CCGCAGC459.672658E-870.587141
GTGTGGC55650.069.667577
CGGGGCA14250.067.5234894
GGGCCCG11100.066.388448
TTCACCG170150.066.3167
CGCTGCT800.066.175441
CGGGCCC11300.065.2134257
CGTAGTG25650.065.14464
AACGGGC11150.064.40695
CAGCTGC1250.063.528421
GGCCCGA11800.062.052389
ACCTTCA66850.061.9976354
ATGCAGC702.1827873E-1160.3502582
GGCTGCC802.3646862E-1159.5578961
TGTGGCA65750.059.4654968
ACGGGCC12250.058.6234256
Produced by FastQC (version 0.11.5)